Electron microscopic study of Bluegill virus

1982 ◽  
Vol 28 (4) ◽  
pp. 398-402 ◽  
Author(s):  
Laurent Berthiaume ◽  
Jean Robin ◽  
Robert Alain
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Rna Viruses ◽  
Physical Appearance ◽  
Negative Staining ◽  
Electron Microscopic ◽  
Bunyaviridae Family ◽  
Infected Cells ◽  
Ultrathin Sections

Bluegill virus (BGV) grown in BF-2 cells was studied by negative staining and ultrathin sections of infected cells. Although BGV resembles bunyaviruses in gross physical appearance, it differs from this group in several important aspects. Thus, BGV cannot be classified as a member of the Bunyaviridae family and could be a representative of a novel family of enveloped RNA viruses.

Electron microscopic study of respiratory syncitial (RS) virus: A new approach

1988 ◽  
Vol 46 ◽  
pp. 312-313
Author(s):  
R. Alain ◽  
F. Bélanger ◽  
L. Berthiaume ◽  
M. Trudel
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Post Infection ◽  
Final Concentration ◽  
Negative Staining ◽  
Electron Microscopic ◽  
New Approach ◽  
Ok Cells ◽  
Ultrathin Sections

The respiratory syncytial (RS) virus is included in the Paramyxoviridae family, genus Pneumovirus. In the present study, three strains were compared: human (HRS), bovine (BRS) and caprine (CRS). The ultrastructure of HRS virus has been previously described according to conventional methods of negative staining and ultrathin sectioning (Berthiaume et al., 1974). Recently, bridges between BRS particles in ultrathin sections were noted (Bélanger et al., 1988). Bridges were also observed with CRS (unpublished results). We were interested in studying, by negative staining, what produced these bridges and why they were present on BRS and CRS particles and not on HRS.Human, bovine and caprine RS virus were propagated in ovine kidney (OK) cells. After 5 to 7 days post-infection, when viral titers were at a maximum, supernatants were collected. They were separated in two parts: the first aliquot was used without treatment immediately after collection; the second aliquot was immediately fixed with glutaraldehyde (final concentration of 0,5% (v/v)) and after ten minutes fixation, samples were prepared for electron microscopy.

Electron Microscopic Study of the Morphogenesis of Echovirus 23

1973 ◽  
Vol 31 ◽  
pp. 590-591
Author(s):  
C. M. Trant ◽  
R. M. Jamison
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Post Infection ◽  
Glass Surface ◽  
Kidney Cells ◽  
Electron Microscopic ◽  
Monolayer Cultures ◽  
Infected Cells ◽  
Infectious Cycle

The cytopathology which accompanies the replication of Echovirus 23 in monkey kidney cells has been studied. Cells grown in monolayer cultures in glass bottles were infected with Echovirus 23 and incubated at 37°C. At varying intervals, cells were harvested for electron microscopy. Multiple embeddings of specimens at each interval post-infection were performed to confirm and extend the observations. It was noted that as the interval postinfection lengthened, individual infected cells rounded and detached from the surface of the monolayer. As the infectious cycle continued, virtually all cells in the bottle became detached. Accordingly, each infected bottle provided two specimens for electron microscopy; a) those free in the supernatent. fluid and b) those remaining on.the glass surface.

Electron-microscopic study of Herpesvirus macaca in human fibroblasts

1976 ◽  
Vol 22 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Peter R. Graze ◽  
Phillip McGrath ◽  
Glenelle C. Washington ◽  
Ivor Royston
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Human Fibroblasts ◽  
Infectious Agent ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Enveloped Virus ◽  
Negative Stain ◽  
Infected Cells ◽  
Myelin Figures

An electron microscopic study of the morphology of Herpesvirus macaca, a serologically distinct infectious agent isolated from the leukocytes of rhesus monkeys, was performed. WI-38 fibroblast monolayers were infected with the virus and examined 18 days later. The morphology of Herpesvirus macaca was, in general, typical of the herpesvirus group. Enveloped virus particles observed via negative-stain technique had a diameter of 145–155 nm. An inner capsid composed of hexagonal capsomeres had a diameter of 100–110 nm and surrounded a central core. While enveloped forms appeared to be present within the nuclei of infected cells, they were not found in the cytoplasm except within vacuolar structures. Associated changes were found in the morphology of infected cells, including intracytoplasmic myelin figures.

Electron Microscopic Study of Hemadsorption on Vaccinia Virus Infected Cells

1977 ◽  
Vol 21 (10) ◽  
pp. 593-600
Author(s):  
Yasuo Saburi ◽  
Kenji Okuda ◽  
Toyozoh Takahashi ◽  
Ichiro Tadokoro
Keyword(s):  
Vaccinia Virus ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Electron Microscopic ◽  
Infected Cells
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