The use of the negative staining method for the electron-microscopic study of subcellular particles from animal tissues

1962 ◽  
Vol 58 (1) ◽  
pp. 1-16 ◽  
Author(s):  
R. W. Horne ◽  
V. P. Whittaker
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Staining Method ◽  
Negative Staining ◽  
Animal Tissues ◽  
Electron Microscopic

Electron microscopic study of respiratory syncitial (RS) virus: A new approach

1988 ◽  
Vol 46 ◽  
pp. 312-313
Author(s):  
R. Alain ◽  
F. Bélanger ◽  
L. Berthiaume ◽  
M. Trudel
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Post Infection ◽  
Final Concentration ◽  
Negative Staining ◽  
Electron Microscopic ◽  
New Approach ◽  
Ok Cells ◽  
Ultrathin Sections

The respiratory syncytial (RS) virus is included in the Paramyxoviridae family, genus Pneumovirus. In the present study, three strains were compared: human (HRS), bovine (BRS) and caprine (CRS). The ultrastructure of HRS virus has been previously described according to conventional methods of negative staining and ultrathin sectioning (Berthiaume et al., 1974). Recently, bridges between BRS particles in ultrathin sections were noted (Bélanger et al., 1988). Bridges were also observed with CRS (unpublished results). We were interested in studying, by negative staining, what produced these bridges and why they were present on BRS and CRS particles and not on HRS.Human, bovine and caprine RS virus were propagated in ovine kidney (OK) cells. After 5 to 7 days post-infection, when viral titers were at a maximum, supernatants were collected. They were separated in two parts: the first aliquot was used without treatment immediately after collection; the second aliquot was immediately fixed with glutaraldehyde (final concentration of 0,5% (v/v)) and after ten minutes fixation, samples were prepared for electron microscopy.

Electron microscopic study of Bluegill virus

1982 ◽  
Vol 28 (4) ◽  
pp. 398-402 ◽  
Author(s):  
Laurent Berthiaume ◽  
Jean Robin ◽  
Robert Alain
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Rna Viruses ◽  
Physical Appearance ◽  
Negative Staining ◽  
Electron Microscopic ◽  
Bunyaviridae Family ◽  
Infected Cells ◽  
Ultrathin Sections

Bluegill virus (BGV) grown in BF-2 cells was studied by negative staining and ultrathin sections of infected cells. Although BGV resembles bunyaviruses in gross physical appearance, it differs from this group in several important aspects. Thus, BGV cannot be classified as a member of the Bunyaviridae family and could be a representative of a novel family of enveloped RNA viruses.

The Growth of Herpesvirus mulatto in Human Fibroblast

1974 ◽  
Vol 32 ◽  
pp. 244-245
Author(s):  
Glennelle Washington ◽  
Philip P. McGrath ◽  
Peter R. Graze ◽  
Ivor Royston
Keyword(s):  
Rhesus Monkey ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Peripheral Blood ◽  
Human Fibroblast ◽  
Rhesus Monkeys ◽  
Human Fibroblasts ◽  
Electron Microscopic ◽  
Specific Antisera ◽  
Peripheral Blood Leucocytes

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

1971 ◽  
Vol 29 ◽  
pp. 338-339
Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall
Keyword(s):  
Cell Wall ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Genetic Heterogeneity ◽  
Morphological Characteristics ◽  
Electron Microscopic ◽  
Dna Reassociation ◽  
Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

A New Technique for the Light and Electron Microscopic Study of Individual Cerebro-Spinal Fluid Cells in a Mona Plane Layer

1976 ◽  
Vol 34 ◽  
pp. 362-363
Author(s):  
L.A. Dell
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Plane Layer ◽  
Ultrastructural Level ◽  
Spinal Fluid ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Easy Method ◽  
Cell Pellet ◽  
A New Technique

A new method has been developed which readily offers the microscopist a possibility for both light and electron microscopic study of selected cells from the cerebrospinal fluid. Previous attempts to examine these cells in the spinal fluid at the ultrastructural level were based on modifications of cell pellet techniques developed for peripheral blood. These earlier methods were limited in application by the number of cells in spinal fluid required to obtain a sufficient size pellet and by the lack of an easy method of cellular identification between the light and electron microscopic level. The newly developed method routinely employs microscope slides coated with Siliclad and tungsten oxide for duplicate cytocentrifuge preparations of diagnostic spinal fluid specimens. Work done by Kushida and Suzuki provided a basis for our use of the metal oxide.

Vesicular system associated with spermiogenesis of bullfrog

1987 ◽  
Vol 45 ◽  
pp. 818-819
Author(s):  
K. C. Liu ◽  
S. F. Tsay
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Reproductive System ◽  
Seminiferous Tubule ◽  
Rana Catesbeiana ◽  
Routine Procedure ◽  
Electron Microscopic ◽  
Twisted Tubules ◽  
Electron Lucent

In the histologic and electron microscopic study of the male reproductive system of bullfrog, Rana catesbeiana, a vesicular system associated with spermiogenesis was observed. It appeared in the lumenal space of the seminiferous tubule (Fig. 1), in the heads of spermatids (Fig. 2), associated with the chromatins of the spermatid (Fig. 4). As deduced from sections, this vesicular system consisted of vesicles of various size or a large group of waving and twisted tubules (Fig. 3), After routine procedure of treatment for electron microscopy, the lumens of both of the vesicles and tubules were electron lucent.In human, vesicles and vesicular system associated with reproductive cell and tissue were reported. In abnormal spermiogenesis, flower-like body, actually vesicles, and giant vesicle associated with the head of spermatid were observed. In both cases the number of vesicle was limited from a single one to a few.

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