Use of fluorescence microscopy for monitoring periodontal disease state

1981 ◽  
Vol 27 (7) ◽  
pp. 675-678 ◽  
Author(s):  
E. C. S. Chan ◽  
J. de Vries ◽  
R. F. Harvey ◽  
Y.-C. Tam
Keyword(s):  
New York ◽  
Periodontal Disease ◽  
Rapid Detection ◽  
Healthy Subjects ◽  
Bacteroides Fragilis ◽  
Dark Field ◽  
Disease State ◽  
Dark Field Microscopy ◽  
Significant Difference ◽  
And Control

Samples of subgingival plaque from patients with periodontal disease and control subjects were stained with the Fluoretec fluorescent test kits (Pfizer Inc., New York) developed for the rapid detection of members of the Bacteroides fragilis and B. melanino genicus groups of anaerobes. The same fluorescent fields were also examined by dark-field microscopy for the total count of bacteria. Bacteroides fragilis and B. melaninogenicus were found in plaque samples of healthy subjects and periodontally diseased patients with no significant difference in percent of total flora. Oral spirochetes also fluoresced with the antisera used. Samples from healthy sites showed virtually no spirochetes; spirochetes were present in diseased sites. Tests with other antisera also showed that fluorescein-labelled antibodies can be absorbed nonspecifically to the surface of spirochetes. Such a phenomenon can be used to monitor an individual's periodontal disease state.

scholarly journals Evaluation of the phagocytic activity of peripheral blood monocytes of patients with Jorge Lobo's disease

2004 ◽  
Vol 37 (2) ◽  
pp. 165-168 ◽  
Author(s):  
Fátima Regina Vilani-Moreno ◽  
Luciana Moreira Silva ◽  
Diltor Vladimir Araújo Opromolla
Keyword(s):  
Incubation Time ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Healthy Subjects ◽  
Mononuclear Cells ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Significant Difference ◽  
Host Parasite ◽  
And Control

Studies on host-parasite interaction in Jorge Lobo's disease are scarce, with no report in the literature on the phagocytosis of Lacazia loboi by phagocytic mononuclear cells. Thus, the objective of the present study was to assess the phagocytic activity of blood monocytes in the presence of L. loboi in patients with the disease and in healthy subjects (controls) over 3 and 24 hours of incubation. Statistical analyses of the results showed no significant difference in percent phagocytosis of the fungus between patient and control monocytes. With respect to incubation time, however, there was a significant difference, in that percent phagocytosis was higher at 3 hours than at 24 hours (p <0.01). These results suggest that monocytes from patients with the mycosis are able to phagocyte the fungus, as also observed in control individuals.

scholarly journals A COMPARATIVE STUDY TO ASSES THE EFFICACY OF NANOBIO FUSION GEL AS AN ADJUNCT TO SCALING AND POLISHING IN CHRONIC PERIODONTITIS. - A CLINICAL STUDY

2020 ◽  
pp. 1-3
Author(s):  
Patil A. Veena ◽  
Ansari T. Sobia ◽  
Agarwal Priyanka ◽  
Ayesha Ayesha ◽  
Sultana Shahnaaz
Keyword(s):  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Antimicrobial Agents ◽  
Test Group ◽  
Controlled Clinical Trial ◽  
Pocket Depth ◽  
Antioxidant Power ◽  
Probing Pocket Depth ◽  
Significant Difference ◽  
And Control

Introduction: Various chemical agents such as nonsteroidal, anti-inflammatory drugs and antimicrobial agents has gained popularity in treatment of periodontal disease but simultaneously lead to condition such as drug resistance and drug allergy. Hence , the topical application of herbal agents such as propolis, aloevera, green tea extracts, Neem reduces the potency and effectiveness to prevent progression of periodontal disease. NanoBioFusion(NBF)gel contains the natural antioxidant power of propolis,vit C,vit E which allows the ultrafine antioxidant to surpase the moist intraoral environment to enter the cells and rejuvenate,revitalize,support,protect and optimize gum and soft oral tissue.Hence the present study is aimed to evaluate the clinical effect of locally delivered NBF gel as an adjunctive therapy to scaling and polishing in the treatment of Periodontitis. Materials and Methods: Chronic Periodontitis patients with 40 sites and probing pocket depth (PD) between 5 and 7 mm were selected in a randomized controlled clinical trial. SRP was performed in both control and test group followed by NBF gel application in 40 sites. The plaque index, gingival index and probing Pocket depth,were recorded at baseline, 6 weeks, and 3 months.The statistical analysis with paired t‑test was used to compare the test and control sites. Results: From baseline to a period of 3 months, a statistically significant difference was seen between both groups for Pocket probing depth and from baseline to 6 weeks the mean GI and PI score have a statistically significant result was obtained(P=0.01& 0.00). Conclusions: Locally delivered NBF gel exhibited a significant improvement compared with SRP alone in chronic periodontitis.

Increased NOX1 and DOUX2 Expression in the Colonic Mucosa of Patients with Chronic Functional Constipation

2021 ◽  
Author(s):  
Xiuqin Wei ◽  
Chunbo Kang ◽  
Lei Gao ◽  
Mengqiao Zhang ◽  
Mei Xue ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Healthy Subjects ◽  
Mrna Level ◽  
Functional Constipation ◽  
Inflammatory Cells ◽  
Histological Structure ◽  
Mrna Levels ◽  
Significant Difference ◽  
And Control ◽  
Colonic Biopsies

Abstract Aim To determine whether oxidative stress and inflammation are associated with constipation by examining the expression of the main producers of reactive oxygen species, NADPH oxidases, and pro-inflammatory cytokines in the colon of patients with chronic functional constipation. Methods The colonic biopsies were collected from 32 patients with chronic functional constipation and 30 healthy subjects who underwent colonoscopy. Colonic mucosal histology was observed. IL-1β, IL-6, IL-8 mRNA, and four members of NADPH oxidase (NOX1, NOX2, DOUX2 and NOX4) protein and mRNA were assessed by immunohistochemistry, western blotting and RT-PCR. Results The tissues from both patients and healthy subjects showed normal histological structure without increase of inflammatory cells. NOX1 protein and mRNA levels were significantly increased compared to controls (P<0.05). DOUX2 protein, but not mRNA, was increased by twofold compared to controls (P<0.05). The levels of NOX2 and NOX4 protein and mRNA demonstrated no significant difference between patients and control subjects. The levels of IL-1β and IL-6 mRNA were significantly higher in constipation patients (P<0.05), while IL-8 mRNA level was no different between the two groups. Conclusion NADPH oxidase and pro-inflammatory cytokine might be involved in the pathogeneses of chronic functional constipation.

Discolouration and decay associated with paraformaldehyde-treated tapholes in sugar maple

1978 ◽  
Vol 8 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Russell S. Walters ◽  
Alex L. Shigo
Keyword(s):  
New York ◽  
Sugar Maple ◽  
Rapid Development ◽  
Mature Trees ◽  
Total Length ◽  
Sugar Maples ◽  
Significant Difference ◽  
Final Measurement ◽  
Repeated Use ◽  
And Control

More decay (higher incidence and greater total length of column) was associated with tapholes in mature sugar maples (Acersaccharum Marsh.) treated with a 250-mg paraformaldehyde pill than with control tapholes. This was apparent 20 months after treatment and at each successive examination to the final measurement at 56 months. Discoloured columns associated with pill-treated tapholes were longer than those associated with control tapholes for the first 8 months. From that time until the final measurement there were no statistically significant differences between lengths of discoloured columns associated with pill-treated and control tapholes. Cambial dieback occurred adjacent to many tapholes but there was no significant difference in closure rates of treated and control tapholes. Results were obtained from dissections and studies of 180 mature trees over a 56-month period in six locations in Vermont in one experiment, and from 75 trees over a 20-month period in three locations each in Vermont, Maine, New York, Pennsylvania, and Michigan in another experiment. The results indicate that repeated use of paraformaldehyde will lead to rapid development of decay in sugar maple trees.

Effects of Culture Supernatants of Leukocytes from Periodontal Patients on Monocyte-Mediated Hydroxyapatite Resorption

1988 ◽  
Vol 2 (2) ◽  
pp. 368-371
Author(s):  
Y. Marumoto ◽  
I. Sato ◽  
K. Ikeda
Keyword(s):  
Periodontal Disease ◽  
Peripheral Blood ◽  
Healthy Subjects ◽  
Mononuclear Cells ◽  
Healthy Controls ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Significant Difference ◽  
Culture Supernatants

In this study, the effects of culture supernatants on various activities of the monocyte, as a bone-resorbing cell, were compared between peripheral blood leukocyte (PBL) cultures from patients with periodontal disease and those from subjects with a clinically healthy periodontium. We have reported that normal human monocytes in vitro induce the release of calcium from synthetic hydroxyapatite particles and that the activity is enhanced by supernatants from cultures of stimulated or non-stimulated peripheral blood leukocytes. Monocytes from both patients and healthy subjects induced the release of calcium from hydroxyapatite particles (HA) to an equal degree. This activity of monocytes from healthy subjects showed a statistically significant increase by addition of supernatants from stimulated or unstimulated cultures of peripheral blood leukocytes from periodontitis patients. This increase was greater than that seen with supernatants from cells of healthy controls. The Nitro Blue Tetrazolium reduction activity and [3H]-thymidine incorporation of monocytes were also increased by addition of the supernatants from leukocyte cultures from either patients or healthy controls, but no significant difference was noted in the increase. These results suggest that the HA-resorbing activity of monocytes was enhanced by factors from cultured leukocytes. Furthermore, these studies showed that production of these factors by peripheral mononuclear cells from patients with periodontal disease was greater than that seen with cells from normal subjects.

Vestibular click-evoked myogenic potentials: sensitivity and factors determining abnormality in patients with multiple sclerosis

2007 ◽  
Vol 13 (2) ◽  
pp. 193-198 ◽  
Author(s):  
T Patkó ◽  
M Simó ◽  
Z Arányi
Keyword(s):  
Multiple Sclerosis ◽  
Healthy Subjects ◽  
Disease Duration ◽  
Clinical Signs ◽  
Vestibular Evoked Myogenic Potential ◽  
Significant Difference ◽  
Electrophysiological Method ◽  
Spinal Pathway ◽  
And Control ◽  
Vestibular Pathways

Vestibular evoked myogenic potential (VEMP) assesses the sacculo-spinal pathway. The aim of our study was to examine sensitivity and factors determining abnormality of VEMP, indicative of brainstem dysfunction, in patients with multiple sclerosis (MS). Thirty healthy subjects and 30 MS patients were examined. All healthy subjects showed a normal biphasic response. Twelve of the 30 MS patients (40%) had abnormal recordings. There was a significant difference between MS patients and control subjects with respect to P13 latency (longer in the MS group) and P13-N23 amplitude (lower in the MS group). VEMP abnormalities were statistically significantly related to the presence of brainstem demyelinative lesions and a weaker correlation was found with disease duration. Clinical signs of vestibular dysfunction at any point during the course of the disease did not seem to affect the chances of obtaining abnormal VEMPs. Although the sensitivity of VEMP in detecting abnormality in MS patients is relatively low, its significance is evident in that it is the only electrophysiological method that is able to detect dysfunction in central vestibular pathways. Multiple Sclerosis 2007; 13: 193–198. http://msj.sagepub.com

scholarly journals Serum interleukin-17 (IL-17) in chronic periodontitis patients

2017 ◽  
Vol 29 (3) ◽  
Author(s):  
Erry Mochamad Arief ◽  
Marina Binti Mubin ◽  
Siti Lailatul Akmar Zainuddin ◽  
Nurul Asma Abdullah ◽  
Basaruddin Ahmad
Keyword(s):  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Healthy Subjects ◽  
Elisa Test ◽  
Treatment Center ◽  
Interleukin 17 ◽  
Chronic Periodontal Disease ◽  
Screening Assessment ◽  
And Control ◽  
Control Study

Introduction: Explorations into the periodontal medicine relationship have discovered interleukin-17 (IL-17) cytokine as one of the pro-inflammatory mediators that play a crucial role in the initiation and progression of the chronic periodontal disease. This case and control study aimed to compare the serum IL-17 concentration between chronic periodontitis and healthy subjects and to assess the relationship between the IL-17 serum and the clinical periodontal parameters in chronic periodontitis patients. Methods: This study was a case-control study. The subjects were selected using purposive sampling method. The periodontal screening assessment in the Family Treatment Center and Postgraduate Dental Clinic, Universiti Sains Malaysia Hospital. A total of 55 samples who fit with the inclusion and exclusion study criteria of full-mouth clinical periodontal records together with blood serums were obtained from 28 chronic periodontitis patients and from 27 healthy subjects. The concentration of IL-17 cytokine in serum was measured using ELISA test. Results: Subjects with periodontal disease presented significantly worse clinical parameters (p<0.001) compared to control. The level of serum IL-17 concentration was significantly higher (p=0.026) in chronic periodontitis subjects 3.6 (1.03) pg/mL compared to the control 3.1 (0.70) pg/mL. Conclusions: There was no correlation between the level of IL-17 concentration in the serum and clinical periodontal parameters.

scholarly journals CLU Polymorphisms in Patients with Pseudoexfoliation Syndrome in Polish Population

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Hanna Lesiewska ◽  
Katarzyna Linkowska ◽  
Joanna Stafiej ◽  
Tomasz Grzybowski ◽  
Jacek Swobodziński ◽  
...  
Keyword(s):  
Allele Frequency ◽  
Disease State ◽  
Pseudoexfoliation Syndrome ◽  
Polish Population ◽  
Control Groups ◽  
Control Subjects ◽  
Genotype Frequencies ◽  
Significant Difference ◽  
And Control

Purpose. To evaluate CLU polymorphisms in patients with pseudoexfoliation syndrome. Materials and Methods. We studied 81 patients (23 males and 58 females, the median age 76 years) and 91 control subjects (27 males and 64 females, the median age 75 years). Genotypes of the CLU polymorphisms (SNPs), rs3087554 and rs2279590, were determined using a commercially available validated genotyping assays. The χ2 test was performed to compare patient and control groups for possible associations between SNP genotype/allele frequency and disease state. Results. There were no significant differences for both allele and genotype frequencies between PEX patients and controls for rs3087554 and rs2279590 polymorphisms. The haplotypes distribution shows statistically significant difference between groups p=0.03. The haplotype (CT) more often was found in controls than in PEX patients, conferring an 18-fold decreased risk to the disease. Conclusion. Our results indicate that CLU variants may contribute to the risk of PEX in the Polish population.

A rapid approach to assess cardiac contractility by ballistocardiogram and electrocardiogram

2018 ◽  
Vol 63 (2) ◽  
pp. 113-122 ◽  
Author(s):  
Xianwen Zhang ◽  
Liyan Zhang ◽  
Kun Wang ◽  
Chao Yu ◽  
Tiangang Zhu ◽  
...  
Keyword(s):  
New York ◽  
Correlation Coefficient ◽  
Healthy Subjects ◽  
Rapid Assessment ◽  
Cardiac Contractility ◽  
Heart Association ◽  
Time Interval ◽  
Significant Difference ◽  
New York Heart Association ◽  
Systolic Time

AbstractIn this paper, we propose a rapid assessment on cardiac contractility by using the time interval between the I wave of ballistocardiogram (BCG) and the R wave of electrocardiogram (ECG) which is referred to as the RI interval. The whole work can be divided into two parts. First, the correlation between the RI interval and the ejection fraction (EF), which is a clinical index to assess systolic performance, was computed. For 39 subjects, the correlation coefficient is −0.54 (p<0.001). Moreover, RI intervals of heart failure (HF) patients and healthy subjects were measured, and a significant difference was found among different New York Heart Association (NYHA) classes and the healthy group. Second, the beat-to-beat correlation analysis between the RI interval and the pre-ejection period (PEP), which is a parameter of systolic time interval to evaluate the cardiac contractility, was calculated. For 4578 heart beats across eight healthy subjects, the correlation coefficient is 0.85 (p<0.001). As a conclusion, these results indicate that the RI interval can be used as a noninvasive assessment of cardiac contractility.

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