Germination of macroconidia and growth of Sporidesmium sclerotivorum in vitro

1981 ◽  
Vol 27 (7) ◽  
pp. 664-669 ◽  
Author(s):  
W. A. Ayers ◽  
E. A. Barnett ◽  
P. B. Adams
Keyword(s):  
Organic Nitrogen ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Mineral Salts ◽  
Ethanolic Extracts ◽  
Carbon Substrates ◽  
Agar Media ◽  
Casamino Acids ◽  
Inorganic And Organic Nitrogen

Macroconidia of Sporidesmium sclerotivorum, a mycoparasite of Sclerotinia spp., were induced to germinte by aqueous and ethanolic extracts of sclerotia of Sclerotinia minor. Paper chromatography of sclerotial extracts indicated the presence of several amino acids and carbohydrates, chiefly glucose. Glucose was identified as the principal germination stimulant in ethanolic extracts. Glucose, fructose, mannose, cellobiose, sucrose, maltose, trehalose, soluble starch, and glycerol at 0.1% (w/v) stimulated macroconidia to germinate in 3–6 days at 25 °C. Crude sclerotial extracts, and glucose combined with inorganic and organic nitrogen sources, supported germination of greater numbers of macroconidia than glucose alone. Yeast extract, Casaminc acids, peptone, and several carbon substrates alone did not support germination. Macroconidia germinated well (> 30%) over the range of pH 3–7; maximum germination (> 80%) occurred at pH 5.0–5.5. Mycelial growth in a glucose – Casamino acids - mineral salts medium was also greatest in the range of pH 5.0–5.5, but growth fell off sharply below pH 4.5 and above pH 6.0. The fungus grew slowly on several complex agar media adjusted to pH 5.5.

OPTIMIZATION OF BIOVANILLIN PRODUCTION OF LEMONGRASS LEAVES HYDROLYSATES THROUGH PHANEROCHAETE CHRYSOSPORIUM

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Huszalina Hussin ◽  
Madihah Md Salleh ◽  
Chong Chun Siong ◽  
Muhammad Abu Naser ◽  
Suraini Abd- Aziz ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Nitrogen Source ◽  
Phanerochaete Chrysosporium ◽  
Organic Nitrogen ◽  
Nitrogen Sources ◽  
Optimum Conditions ◽  
Molar Yield ◽  
Primary Screening ◽  
Organic Nitrogen Source ◽  
Inorganic And Organic Nitrogen

The recent study has demonstrated the effects of different nitrogen sources on vanillin production by Phanerochaete chrysosporium. Primary screening supported maximum biotransformation of ferulic acid (from lemongrass leaves hydrolysate) to vanillin by using ammonium chloride and yeast extract as inorganic and organic nitrogen source, respectively. With the 2-level factorial analysis, the optimum conditions of vanillin production from ferulic acid by P. chrysosporium was achieved at 0.192g/L with a molar yield of 24.5%.

INTEGRATED EFFECT OF INORGANIC AND ORGANIC NITROGEN SOURCES ON SOIL FERTILITY AND PRODUCTIVITY OF MAIZE

2012 ◽  
Vol 35 (4) ◽  
pp. 524-537 ◽  
Author(s):  
Mohammad Shafi ◽  
Azam Shah ◽  
Jehan Bakht ◽  
Mahmood Shah ◽  
Wisal Mohammad
Keyword(s):  
Soil Fertility ◽  
Organic Nitrogen ◽  
Nitrogen Sources ◽  
Inorganic And Organic Nitrogen ◽  
Inorganic And Organic

scholarly journals Biodegradation of propiconazole by Pseudomonas putida isolated from tea rhizosphere

2009 ◽  
Vol 55 (No. 5) ◽  
pp. 196-201 ◽  
Author(s):  
S. Sarkar ◽  
S. Seenivasan ◽  
R. Premkumar
Keyword(s):  
Pseudomonas Putida ◽  
Ammonium Nitrate ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Nutritional Requirements ◽  
Mineral Salts ◽  
Tolerance Level ◽  
Triazole Fungicide ◽  
Growth Promoting

Biodegradation of triazole fungicide propiconazole was carried out <I>in vitro</I> by selected <I>Pseudomonas</I> strains isolated from tea rhizosphere. A total number of twelve strains were isolated and further screened based on their tolerance level to propiconazole. Four best strains were selected and further tested for their nutritional requirements. Among the different carbon sources tested glucose exhibited the highest growth promoting capacity and among nitrogen sources ammonium nitrate supported the growth to the maximum. The four selected <I>Pseudomonas</I> strains exhibited a range of degradation capabilities. Mineral salts medium (MSM) amended with glucose provided better environment for degradation with the highest degradation potential in strain MPR 4 followed by MPR 12 (72.8% and 67.8%, respectively).

Nutritional and environmental factors affecting growth and sporulation of Sporidesmium sclerotivorum

1981 ◽  
Vol 27 (7) ◽  
pp. 685-691 ◽  
Author(s):  
E. A. Barnett ◽  
W. A. Ayers
Keyword(s):  
Nitrogen Source ◽  
Agar Medium ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Mineral Salts ◽  
Factors Affecting ◽  
Carbon And Nitrogen ◽  
Naoh Solution ◽  
Casamino Acids ◽  
Nutritional Studies

Three of five isolates of Sporidesmium sclerotivorum, a mycoparasite of Sclerotinia spp., grew well on an agar medium containing mineral salts, glucose, thiamine, and glutamine or Casamino acids as the nitrogen source. The nitrogen requirement for two of the isolates was satisfied by NH4Cl, Casamino acids, or glutamine. Glutamine was the best single nitrogen source. Only one isolate, CS-1, was used in further nutritional studies. The optimum concentration of glutamine for growth was 5 g/L. Glucose, mannose, mannitol, and cellobiose were excellent carbon sources. A glucose concentration of 20 g/L was optimum. Mannitol supported greater growth than glucose with Casamino acids as the nitrogen source but glucose was the superior carbon source with glutamine as the nitrogen source. Greatest growth was achieved with a combination of these carbon and nitrogen sources. Sporidesmium sclerotivorum, isolate CS-1, required thiamine for growth and sporulation. Biotin stimulated growth. The fungus developed maximally within the range of pH 5.0–5.5 and growth was greatly reduced at a pH below 4.0 or above 6.0. Control of acidity by the periodic addition of NaOH solution permitted substantially increased growth. The optimum temperature for growth was 22.5–25.0 °C but production of macroconidia was greatest at 15–20 °C.

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