Dependence of extracellular chitinase activity of Serratia marcescens QMB1466 on continuous culture dilution rate

1981 ◽  
Vol 27 (1) ◽  
pp. 142-144 ◽  
Author(s):  
Manuel E. Young ◽  
Paul A. Carroad
Keyword(s):  
Enzyme Activity ◽  
Serratia Marcescens ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Culture Filtrate ◽  
Catabolite Repression ◽  
Chitinase Activity

The production of chitinase by Serratia marcescens QMB1466 was studied in continuous culture with N-acetylglucosamine as limiting substrate. Enzyme activity in the culture filtrate exhibited a maximum with respect to dilution rate, a phenomenon that can be explained as a balance between induction and catabolite repression.

CONTINUOUS CULTURE OF BRUCELLA ABORTUS S.19

1961 ◽  
Vol 7 (4) ◽  
pp. 491-505 ◽  
Author(s):  
Andreas H. W. Hauschild ◽  
Hilliard Pivnick
Keyword(s):  
Growth Rate ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Brucella Abortus ◽  
Specific Growth ◽  
Limiting Factor ◽  
Continuous Growth ◽  
Viable Cells ◽  
Metabolic Products ◽  
Growth Of Bacteria

An apparatus is described for the continuous growth of bacteria. Brucella abortus S.19 has been grown in continuous culture for periods up to 3 weeks with populations up to 2 × 1011viable cells per ml and without the establishment of nonsmooth variants.Concentrations between 3 × 109and 2 × 1011cells per ml could be maintained as a function of the dilution rate without the requirement of a known limiting factor in the medium. In a series of steady-state conditions, the specific growth rate increased steadily up to 0.28 hour−1with decreasing population levels.Incidence of mutants was governed by the dilution rate and could also be reduced by various chelating substances.In continuous growth combined with continuous dialysis, population levels were approximately twice those obtained in continuous growth without dialysis. The effect of dialysis appears to be the continuous removal of growth-limiting metabolic products.

Amylase hyper-producing haploid recombinant strains of Thermomyces lanuginosus obtained by intraspecific protoplast fusion

2000 ◽  
Vol 46 (7) ◽  
pp. 669-673 ◽  
Author(s):  
K Rubinder ◽  
B S Chadha ◽  
S Singh ◽  
H S Saini
Keyword(s):  
Protoplast Fusion ◽  
Culture Filtrate ◽  
Catabolite Repression ◽  
Type Strain ◽  
Wild Type Strain ◽  
Thermomyces Lanuginosus ◽  
Wild Type ◽  
Haploid Strain ◽  
Recombinant Strains ◽  
Specific Activities

Amylase hyper-producing, catabolite-repression-resistant, recombinant strains were produced by intraspecific protoplast fusion of thermophilic fungus Thermomyces lanuginosus strains, using well-characterized, morphological, and 2-deoxy-D-glucose resistant markers. The fusant heterokaryons exhibited enhanced amylase activities as compared to the amylase hyper-producing parental strain (T2). Diploids derived from heterokaryons segregated to stable haploid recombinant strains. In the haploid strain (Tlh 4q), approximately 5-fold higher specific activities of α-amylase and glucoamylase in the culture filtrate were observed as compared to the wild-type strain (W0).Key words: Thermomyces lanuginosus, protoplast fusion, amylase hyper-producing strain, catabolite repression.

scholarly journals Effect of dilution rate on lipopolysaccharide and serum resistance of Neisseria gonorrhoeae grown in continuous culture.

1983 ◽  
Vol 41 (1) ◽  
pp. 74-82 ◽  
Author(s):  
S A Morse ◽  
C S Mintz ◽  
S K Sarafian ◽  
L Bartenstein ◽  
M Bertram ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Continuous Culture ◽  
Dilution Rate ◽  
Serum Resistance

scholarly journals Optimization of Agitation Rate in Bioreactor Increases Chitinase Activity of Serratia marcescens PT6

2020 ◽  
Vol 147 ◽  
pp. 03019
Author(s):  
Amara Faiz Wriahusna ◽  
Niswah Umhudloh Dzakiyya ◽  
Indun Dewi Puspita ◽  
Sri Pudjiraharti
Keyword(s):  
Serratia Marcescens ◽  
Bacterial Growth ◽  
Agar Medium ◽  
Chitinase Activity ◽  
Colloidal Chitin ◽  
Gram Negative Bacteria ◽  
Agitation Rate ◽  
Anova Analysis ◽  
Gram Negative ◽  
Shrimp Pond

Serratia marcescens PT6 is a Gram-negative bacteria isolated from shrimp pond sediment that capable of producing chitinase. This study aimed to observe the effect of agitation rate on growth and chitinase activity of S. marcescens PT-6 in a bioreactor. The production of chitinase was done in 1.5 l bioreactor using colloidal chitin broth at the condition of pH 7, the temperature of 30°C, aeration of 0.04 vvm, and variation of agitation rate (200, 350, 500 rpm). Bacterial growth was measured by colonies counting in agar medium, while chitinase activity was measured by means of colorimetric every day for four days incubation. The results of ANOVA analysis show that the agitation rate had no effect on bacterial growth, but a significant effect (P<0.05) was observed on chitinase activity. The highest growth and chitinase activity were obtained at 200 rpm, with the highest chitinase activity of 0.006 ± 0.001 U/ml was at day-2. This study implies that the optimized agitation rate in the bioreactor increased the chitinase activity produced by S. marcescens PT-6.

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