Reversal of succinate-mediated catabolite repression of alkylsulfatase in Pseudomonas aeruginosa by 2,4-dinitrophenol and by sodium malonate

1978 ◽  
Vol 24 (12) ◽  
pp. 1567-1573 ◽  
Author(s):  
J. W. Fitzgerald ◽  
L. C. Kight-Olliff ◽  
G. J. Stewart ◽  
N. F. Beauchamp
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Induction Period ◽  
Sodium Succinate ◽  
Cell Suspensions ◽  
Atp Content ◽  
Succinate Oxidation ◽  
Sodium Malonate ◽  
Atp Levels ◽  
Sodium Malate ◽  
Stimulation Of

The Kreb's cycle intermediates and related metabolites (e.g., acetate) repressed the induced synthesis of alkylsulfatase in resting cell suspensions of Pseudomonas aeruginosa. At concentrations which caused substantial repression, sodium succinate as well as sodium malate, fumarate, and α-ketoglutarate were oxidized to yield consistently high levels of ATP throughout the induction period. Sodium oxalacetate which was markedly less effective as a metabolite repressor generated high ATP levels only during the first 2 h of the induction period. The addition of 2,4-dinitrophenol or sodium malonate to cell suspensions containing the inducer (sodium hexan-1-yl sulfate) and succinate overcame repression of alkylsulfatase formation and resulted in a reduction in the ATP content to levels found in cells exposed only to inducer. An apparent stimulation of alkylsulfatase induction occurred in the absence of succinate when cells were incubated with 2,4-dinitrophenol and inducer. In this case, the ATP content of the cell suspension fell to levels substantially below those occurring as a result of inducer catabolism. Collectively, these data suggest that the effectiveness of succinate as a metabolite repressor is related to the ATP levels generated as a consequence of succinate oxidation.

Inhibition of enzyme induction in Pseudomonas aeruginosa by exogenous nucleotides

1978 ◽  
Vol 24 (7) ◽  
pp. 811-817 ◽  
Author(s):  
Lynda C. Kight-Olliff ◽  
J. W. Fitzgerald
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cyclic Amp ◽  
Enzyme Induction ◽  
Inhibitory Effect ◽  
Cell Suspensions ◽  
Resting Cell ◽  
Nucleoside Triphosphates ◽  
Stimulation Of

Alkylsulfatase induction in resting cell suspensions of P. aeruginosa was inhibited by exogenously supplied adenosine or by ATP (2 mM). Adenine phosphate had no effect while AMP or ADP caused a slight stimulation of induction. The inhibitory effect of ATP required the presence of added Mg2+, was not reversed by cyclic-AMP(2 mM), and was independent of the nature of the inducer. Of a number of other nucleoside triphosphates tested, only UTP (2 mM) acted as an inhibitor of induction. These nucleotides at external concentrations of 6 mM also inhibited alkylsulfatase induction in actively growing cells.

Effects of ATP precursors on ATP and free ADP content and functional recovery of postischemic hearts

1989 ◽  
Vol 256 (2) ◽  
pp. H560-H566 ◽  
Author(s):  
G. Ambrosio ◽  
W. E. Jacobus ◽  
M. C. Mitchell ◽  
M. R. Litt ◽  
L. C. Becker
Keyword(s):  
Functional Recovery ◽  
Adenine Nucleotide ◽  
Mean Value ◽  
Global Ischemia ◽  
Stunned Myocardium ◽  
Base Line ◽  
Atp Content ◽  
Atp Levels ◽  
Developed Pressure

It has been proposed that administration of adenine nucleotide precursors might accelerate replenishment of myocardial ATP and "free" ADP, thus improving recovery of depressed contractility of postischemic hearts. To test this hypothesis, Langendorff-perfused rabbit hearts were subjected to 20 min of global ischemia and reperfused for 2 h with normal perfusate (n = 8) or perfusate containing 100 mumol/l of the ATP precursors adenosine (n = 8) or 5-amino-4-imidazolecarboxamide riboside (AICAriboside; n = 8). After reperfusion, developed pressure in untreated hearts averaged 70-80% of base line, whereas ATP content was reduced to approximately 70% of preischemic values. AICAriboside administration did not increase tissue ATP levels or contractility. However, in every heart that received adenosine during reperfusion, ATP content increased from a mean value of 65 +/- 4% of base line to 84 +/- 5% at the end of reperfusion (P less than 0.001). Free ADP also increased in adenosine-treated hearts from 40 to 50% of base line at the beginning of reperfusion, to normal levels by 60 min. However, no improvement in contractility was observed in the hearts that received adenosine. These results support the hypothesis that decreased availability of nucleotide precursors is responsible for depressed ATP levels in postischemic hearts; however, reduced ATP and free ADP levels may not be directly responsible for the depressed function of stunned myocardium.

scholarly journals Comparison between Pseudomonas aeruginosa siderophores and desferrioxamine for iron acquisition from ferritin

2010 ◽  
Vol 4 (4) ◽  
pp. 631-635 ◽  
Author(s):  
Somporn Srifuengfung ◽  
Susan Assanasen ◽  
Malulee Tuntawiroon ◽  
Sumonrat Meejanpetch
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Iron Acquisition ◽  
Iron Chelator ◽  
Dialysis Membrane ◽  
In Vitro Experiment ◽  
Iron Mobilization ◽  
Ph Values ◽  
Minimum Medium ◽  
Stimulation Of

Abstract Background: Siderophore is an iron chelator produced by microorganism. Pseudomonas aeruginosa produces two siderophores (pyoverdin and pyochelin). Desferrioxamine is a siderophore used in thalassemia patients to treat an iron overload of vital organs. Objective: Compare the ability of pyoverdin, pyochelin, and desferrioxamine for iron mobilization from ferritin. Materials and Methods: In vitro experiment, the ability of P. aeruginosa siderophores and desferrioxamine for iron mobilization from ferritin was compared by using a dialysis membrane assay at pH values of 7.4 and 6.0. Stimulation of P. aeruginosa PAO1 growth by all siderophores was studied in glucose minimum medium. Results: All three compounds were capable of iron mobilization at both pHs. At pH 6.0, the most effectiveness compound was desferrioxamine (31.6%), followed by pyoverdin (21.5%) and pyochelin (13.7%) compared on weight basis, each at 10 μg/mL. At equimolar concentration, their activities were desferrioxamine (38.5±1.2%), followed by pyoverdin (32.0±4.8%) and pyochelin (26.7±1.9%), respectively. Conclusion: The most effective compound in iron mobilization from ferritin was desferrioxamine, followed by pyoverdin and pyochelin respectively.

Ion Pairing and Solvent Relaxation Processes in Aqueous Solutions of Sodium Malonate and Sodium Succinate

2004 ◽  
Vol 108 (36) ◽  
pp. 13789-13795 ◽  
Author(s):  
Andrew Tromans ◽  
Peter M. May ◽  
Glenn Hefter ◽  
Takaaki Sato ◽  
Richard Buchner
Keyword(s):  
Aqueous Solutions ◽  
Sodium Succinate ◽  
Ion Pairing ◽  
Relaxation Processes ◽  
Solvent Relaxation ◽  
Sodium Malonate

scholarly journals The characteristics and site of inhibition of gluconeogenesis in rat liver cells by bacterial endotoxin. Stimulation of phosphofructokinase-1

1987 ◽  
Vol 242 (3) ◽  
pp. 721-728 ◽  
Author(s):  
R G Knowles ◽  
J P McCabe ◽  
S J Beevers ◽  
C I Pogson
Keyword(s):  
Enzyme Activities ◽  
Pyruvate Carboxylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Liver Cells ◽  
Atp Content ◽  
Rat Liver Cells ◽  
Endogenous Substrates ◽  
And Control ◽  
Control Coefficients ◽  
Stimulation Of

The characteristics and site of inhibition of gluconeogenesis by endotoxin were investigated in liver cells isolated from control and endotoxin-treated rats. Endotoxin treatment was associated with inhibition (40-50%) of gluconeogenesis from lactate plus pyruvate over a range of concentrations of substrate and of oleate and with or without glucose or glucagon. Similar inhibition was observed with asparagine, proline, glutamine, alanine and a substrate mixture, but not with glycerol, glyceraldehyde, dihydroxyacetone or endogenous substrates. There was no change in cellular ATP content or in the rates of ketogenesis or ureogenesis from asparagine, proline or glutamine. Other effects on isotopic fluxes, metabolite contents, enzyme activities and control coefficients were consistent with the suggestion that the effects of endotoxin on gluconeogenesis are exerted at the level of phosphofructokinase-1, and not at phosphoenolpyruvate carboxykinase, pyruvate kinase, pyruvate carboxylase or glucokinase.

scholarly journals Stimulation of β2-adrenergic receptor increases CFTR function and decreases ATP levels in murine hematopoietic stem/progenitor cells

2015 ◽  
Vol 14 (1) ◽  
pp. 26-33 ◽  
Author(s):  
Teresa Trotta ◽  
Lorenzo Guerra ◽  
Donatella Piro ◽  
Maria d'Apolito ◽  
Claudia Piccoli ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Adrenergic Receptor ◽  
Hematopoietic Stem ◽  
Atp Levels ◽  
Β2 Adrenergic Receptor ◽  
Stimulation Of

SUBSTRATE-DEPENDENT PHOSPHORYLATION IN RESTING CELLS OF PSEUDOMONAS AERUGINOSA

1961 ◽  
Vol 7 (1) ◽  
pp. 91-97
Author(s):  
G. A. Strasdine ◽  
J. J. R. Campbell ◽  
H. P. C. Hogenkamp ◽  
J. N. Campbell
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Electron Transport Chain ◽  
Phosphate Uptake ◽  
Growth Yield ◽  
Resting Cells ◽  
Resting Cell ◽  
Transport Chain ◽  
Oxidation Of Glucose ◽  
Phosphate Incorporation ◽  
Stimulation Of

Resting cell suspensions of Pseudomonas aeruginosa exhibited substrate-dependent phosphorylation and most of the phosphate appeared in the nucleic acid fraction. The amount of P32 incorporated was a function of substrate concentration. When equivalent amounts of glucose, gluconate, or 2-ketogluconate were used as substrate, it was found that the more oxidized substrates supported appreciably less P32 incorporation, thus indicating that phosphorylation is coincident with the passage of electrons to oxygen by way of the electron transport chain. These data serve to illustrate that the practice of determining the amount of energy available from the dissimilation of a substrate by measuring growth yield can be in error since equivalent quantities of glucose, gluconate, and 2-ketogluconate supported equal amounts of growth. The P:O ratios obtained with glucose as substrate were of the order of 0.01. Phosphorylation was not sensitive to dinitrophenol or sodium fluoride but was completely inhibited by cyanide. Chloramphenicol, at a concentration which inhibited protein synthesis, caused a twofold stimulation of phosphate incorporation. Pyocyanine, which stops the oxidation of glucose at the 2-ketogluconate stage, completely inhibited phosphate uptake. The action of pyocyanine on both oxidation and phosphorylation could be reversed by magnesium. When extracts of this organism were studied, it was found that under all conditions the addition of oxidizable substrates decreased P32 incorporation.

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