The taxonomy of some new isolates of dissimilatory sulfate-reducing bacteria

1977 ◽  
Vol 23 (10) ◽  
pp. 1415-1425 ◽  
Author(s):  
G. W. Skyring ◽  
H. E. Jones ◽  
D. Goodchild
Keyword(s):  
Papua New Guinea ◽  
Buoyant Density ◽  
Principal Component ◽  
Sulfate Reducing Bacteria ◽  
Similarity Coefficient ◽  
Sulfite Reductase ◽  
Principal Component Factor Analysis ◽  
Sulfate Reducing ◽  
Aps Reductase ◽  
Reducing Bacteria

The interrelationships between 92 isolates of sporing and non-sporing sulfate-reducing bacteria were determined. Of the 116 biochemical and physiological characteristics examined, only 25 were useful for discrimination of groups. Responses to most of the tests were negative. A similarity coefficient and a principal component factor analysis of these data were made. The deoxyribonucleic acids buoyant densities (DNA) from all strains and the electrophoretic properties of adenylylsulfotransferase (ATP-sulfurylase), adenylphosphosulfate (APS)-reductase, and sulfite reductase of selected isolates were determined. On the basis of these various data eight groups were recognized. Isolates of seven of these groups appeared to be similar to one or more named strains. Isolates of group E (DNA buoyant density, 1.708) were different from previously named strains. Sporing strains were not isolated from the Papua New Guinea location. Halophilic and non-halotolerant strains were isolated from highly saline locations in Australia.Results pertinent to the taxonomy and ecology of the sulfate-reducing bacteria are discussed.

A comparison of the electrophoretic properties of the ATP-sulfurylases, APS-reductases, and sulfite reductases from cultures of dissimilatory sulfate-reducing bacteria

1973 ◽  
Vol 19 (3) ◽  
pp. 375-380 ◽  
Author(s):  
G. W. Skyring ◽  
P. A. Trudinger
Keyword(s):  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Disc Electrophoresis ◽  
Sulfite Reductase ◽  
Electrophoretic Variation ◽  
Sulfate Reducing ◽  
Aps Reductase ◽  
Reducing Bacteria ◽  
Atp Sulfurylases

ATP-sulfurylases, APS-reductases, and sulfite reductases (SO3−2 → S−2) have been detected by gel disc electrophoresis in 13 cultures of dissimilatory sulfate-reducing bacteria and their electrophoretic properties have been compared. With respect to these three enzymes only, the results were indicative of some interspecies and intergenus homologies. In the Desulfovibrio strains (except Desulfovibrio desulfuricans 8301 which does not contain desulfoviridin), the major sulfite reductase was electrophoretically coincident with desulfoviridin and, in the Desulfotomaculum strains, with a brown protein. Some distinct patterns of electrophoretically distinguishable forms of APS-reductase were found. Considerable electrophoretic variation was found among the ATP-sulfurylases.

scholarly journals Molecular Ecological Analysis of the Succession and Diversity of Sulfate-Reducing Bacteria in the Mouse Gastrointestinal Tract

2000 ◽  
Vol 66 (5) ◽  
pp. 2166-2174 ◽  
Author(s):  
B. Deplancke ◽  
K. R. Hristova ◽  
H. A. Oakley ◽  
V. J. McCracken ◽  
R. Aminov ◽  
...  
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Sulfate Reducing Bacteria ◽  
Pcr Analysis ◽  
Dot Blot ◽  
Sulfate Reducing ◽  
Aps Reductase ◽  
Mouse Intestine ◽  
Reducing Bacteria ◽  
Old Mice

ABSTRACT Intestinal sulfate-reducing bacteria (SRB) growth and resultant hydrogen sulfide production may damage the gastrointestinal epithelium and thereby contribute to chronic intestinal disorders. However, the ecology and phylogenetic diversity of intestinal dissimilatory SRB populations are poorly understood, and endogenous or exogenous sources of available sulfate are not well defined. The succession of intestinal SRB was therefore compared in inbred C57BL/6J mice using a PCR-based metabolic molecular ecology (MME) approach that targets a conserved region of subunit A of the adenosine-5′-phosphosulfate (APS) reductase gene. The APS reductase-based MME strategy revealed intestinal SRB in the stomach and small intestine of 1-, 4-, and 7-day-old mice and throughout the gastrointestinal tract of 14-, 21-, 30-, 60-, and 90-day-old mice. Phylogenetic analysis of APS reductase amplicons obtained from the stomach, middle small intestine, and cecum of neonatal mice revealed that Desulfotomaculum spp. may be a predominant SRB group in the neonatal mouse intestine. Dot blot hybridizations with SRB-specific 16S ribosomal DNA (rDNA) probes demonstrated SRB colonization of the cecum and colon pre- and postweaning and colonization of the stomach and small intestine of mature mice only. The 16S rDNA hybridization data further demonstrated that SRB populations were most numerous in intestinal regions harboring sulfomucin-containing goblet cells, regardless of age. Reverse transcriptase PCR analysis demonstrated APS reductase mRNA expression in all intestinal segments of 30-day-old mice, including the stomach. These results demonstrate for the first time widespread colonization of the mouse intestine by dissimilatory SRB and evidence of spatial-specific SRB populations and sulfomucin patterns along the gastrointestinal tract.

scholarly journals Phylogeography of Sulfate-Reducing Bacteria among Disturbed Sediments, Disclosed by Analysis of the Dissimilatory Sulfite Reductase Genes (dsrAB)

2005 ◽  
Vol 71 (2) ◽  
pp. 1004-1011 ◽  
Author(s):  
J. R. Pérez-Jiménez ◽  
L. J. Kerkhof
Keyword(s):  
New York ◽  
Distribution Patterns ◽  
Sulfate Reducing Bacteria ◽  
Sulfite Reductase ◽  
Geographic Proximity ◽  
Sulfate Reducing ◽  
Dissimilatory Sulfite Reductase ◽  
Polluted Sites ◽  
Eukaryotic Organisms ◽  
Reducing Bacteria

ABSTRACT Sediment samples were collected worldwide from 16 locations on four continents (in New York, California, New Jersey, Virginia, Puerto Rico, Venezuela, Italy, Latvia, and South Korea) to assess the extent of the diversity and the distribution patterns of sulfate-reducing bacteria (SRB) in contaminated sediments. The SRB communities were examined by terminal restriction fragment (TRF) length polymorphism (TRFLP) analysis of the dissimilatory sulfite reductase genes (dsrAB) with NdeII digests. The fingerprints of dsrAB genes contained a total of 369 fluorescent TRFs, of which <20% were present in the GenBank database. The global sulfidogenic communities appeared to be significantly different among the anthropogenically impacted (petroleum-contaminated) sites, but nearly all were less diverse than pristine habitats, such as mangroves. A global SRB indicator species of petroleum pollution was not identified. However, several dsrAB gene sequences corresponding to hydrocarbon-degrading isolates or consortium members were detected in geographically widely separated polluted sites. Finally, a cluster analysis of the TRFLP fingerprints indicated that many SRB microbial communities were most similar on the basis of close geographic proximity (tens of kilometers). Yet, on larger scales (hundreds to thousands of kilometers) SRB communities could cluster with geographically widely separated sites and not necessarily with the site with the closest proximity. These data demonstrate that SRB populations do not adhere to a biogeographic distribution pattern similar to that of larger eukaryotic organisms, with the greatest species diversity radiating from the Indo-Pacific region. Rather, a patchy SRB distribution is encountered, implying an initially uniform SRB community that has differentiated over time.

scholarly journals Basic Bioelement Contents in Anaerobic Intestinal Sulfate-Reducing Bacteria

2021 ◽  
Vol 11 (3) ◽  
pp. 1152
Author(s):  
Ivan Kushkevych ◽  
Daryna Abdulina ◽  
Dani Dordević ◽  
Monika Rozehnalová ◽  
Monika Vítězová ◽  
...  
Keyword(s):  
Trace Elements ◽  
Trace Metals ◽  
Inductively Coupled Plasma ◽  
Chemical Elements ◽  
Principal Component ◽  
Sulfate Reducing Bacteria ◽  
Bacterial Cells ◽  
Sulfate Reducing ◽  
Total Variability ◽  
Reducing Bacteria

The monitoring of trace metals in microbial cells is relevant for diagnosis of inflammatory bowel disease (IBD). Sulfate-reducing bacteria (SRB) represent an important factor in the IBD development. The content of trace metals in bacterial cells may reflect the functioning of the enzyme systems and the environmental impact on the occurrence of SRB. The aim of our research was to compare the content of trace elements in the cells of SRB cultures isolated from fecal samples of patients with IBD and healthy people. The contents of 11 chemical elements in the bacterial cells of SRB were analyzed by the inductively coupled plasma-mass-spectrometry (ICP-MS) method. Significant changes in the content of calcium, zinc, magnesium, potassium, and iron were observed in patients with IBD compared to healthy individuals. Through a principal component analysis (PCA), a total variability of 67.3% in the difference between the samples was explained. The main factors influencing the total variability in the bacterial cells of SRB isolated from patients suffering from IBD were the content of the micro- and trace elements, such as manganese (with power 0.87), magnesium and cobalt (0.86), calcium (0.84), molybdenum (0.81), and iron (0.78). Such changes in the elemental composition of SRB under different conditions of existence in the host may indicate adaptive responses of the microorganisms, including the inclusion of oxidative stress systems, which can lead to changes in SRB metabolism and the manifestation of parameters of IBD in humans. The use of PCA might make it possible in the future to predict the development and ratio of SRB in patients with various diseases.

scholarly journals Analysis of physiological parameters of Desulfovibrio strains from individuals with colitis

2019 ◽  
Vol 13 (1) ◽  
pp. 481-488 ◽  
Author(s):  
Ivan Kushkevych ◽  
Dani Dordević ◽  
Peter Kollár
Keyword(s):  
Ulcerative Colitis ◽  
Bowel Disease ◽  
Principal Component ◽  
Biomass Accumulation ◽  
Sulfate Reducing Bacteria ◽  
Dissimilatory Sulfate Reduction ◽  
Lactate Oxidation ◽  
Sulfate Reducing ◽  
Sulfide Production ◽  
Reducing Bacteria

AbstractIntestinal sulfate-reducing bacteria are often isolated from patients with inflammatory bowel disease, including ulcerative colitis, and can be involved in the development of gut inflammation. A comparison of the metabolism of intestinal sulfate-reducing bacteria isolated from individuals with colitis and healthy controls using statistical analysis has never been studied and described before. The aim of our research was to evaluate the parameters of dissimilatory sulfate reduction inDesulfovibriospecies that were isolated from the feces of healthy objects and individuals with colitis. Principal component analysis indicates that the strains that were isolated from individuals with colitis grouped in the same cluster by biomass accumulation and sulfide production, same as the strains isolated from healthy individuals. Sulfate and lactate consumption measured over time showed negative correlation (Pearson correlations,p<0.01), healthy: -0.760; colitis: -0.770; healthy: -0.828; colitis: -0.847, respectively. The calculated linear regression (R2) was lower in biomass accumulation and hydrogen sulfide production, 0.531; 0.625 respectively. Thus, biomass accumulation and sulfide production, together with measured kinetic parameters play an important factor in bowel inflammation, including ulcerative colitis. Additionally, acetate production can also synergize with H2S, while sulfate consumption and lactate oxidation likely represent minor factors in bowel disease.

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