A rapid fluorescent focus-inhibition test for determining the neutralizing-antibody response to lymphocytic choriomeningitis virus

1977 ◽  
Vol 23 (5) ◽  
pp. 522-526 ◽  
Author(s):  
W. Lanier Thacker ◽  
Vester J. Lewis ◽  
Gary J. Haller ◽  
George M. Baer
Keyword(s):  
Antibody Response ◽  
Complement Fixation ◽  
Fluorescent Antibody ◽  
Neutralizing Antibody ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Inhibition Test ◽  
Indirect Fluorescent Antibody ◽  
Virus Infections ◽  
Fluorescent Focus

Levels of neutralizing antibody to lymphocytic choriomeningitis (LCM) virus in the sera of 66 infected persons were assayed by a rapid fluorescent focus-inhibition test (RFFIT). The test was more sensitive than the mouse-neutralization (MN) test and could be completed in less than 24 h. The RFFIT titers were compared with titers obtained by the indirect fluorescent-antibody (IFA) and complement-fixation (CF) tests. Neutralizing antibody detected by the RFFIT remained positive after IFA, CF, and MN antibodies had disappeared. The RFFIT for detection of LCM antibody is specific and reproducible and seems especially useful for determining the incidence and epidemiology of LCM virus infections.

scholarly journals Para-influenza 2 virus infections in adult volunteers

1963 ◽  
Vol 61 (4) ◽  
pp. 407-417 ◽  
Author(s):  
D. Taylor-Robinson ◽  
M. L. Bynoe
Keyword(s):  
Antibody Response ◽  
Technical Assistance ◽  
Clinical Evidence ◽  
Complement Fixation ◽  
Haemagglutination Inhibition ◽  
Neutralizing Antibody ◽  
Virus Infections ◽  
Johns Hopkins University ◽  
Antibody Titres ◽  
Adult Volunteers

Twenty-eight adult volunteers were inoculated intranasally with para influenza 2 virus and eight developed illnesses; twenty-eight volunteers were given flanks' saline and one became ill. The illnesses occurred in volunteers given between 2 × 104 and 2 × 106 TCD 50 of virus. The most prominent symptoms were sore throat, nasal stuffiness and coryza; four of the eight volunteers had sufficient coryza to be regarded as having mild colds. Although only eight volunteers had clinical evidence of infection, twenty-four had laboratory evidence of infection as judged by virus re-isolation or antibody response. Neutralization, haemagglutination-inhibition and complement-fixation tests on paired sera showed that sixteen individuals had a fourfold or greater antibody response by one or more tests including five of the eight volunteers who were ill. Twenty volunteers, including seven who were ill, had reciprocal neutralizing antibody titres of eight or more before inoculation of virus so it seems that the illnesses were due to re infection in the presence of antibody. Evidence is presented which suggests that although illnesses occurred in the presence of antibody they were due to the para influenza 2 virus and not some other agent in the inoculum. The results of these experiments seem to fulifi the third of Koch's postulates for para-influenza 2 virus as a cause of respiratory disease in adults.We wish to thank Dr P. A. J. Tyrreil for his advice during the course of this work and in the preparation of the manuscript. We also thank Dr K. V. Shah (The Johns Hopkins University, Baltimore, U.S.A.) for help in some of the early experiments and Miss B. Ridgwell for valuable technical assistance.

Viral escape from the neutralizing antibody response: the lymphocytic choriomeningitis virus model

2001 ◽  
Vol 53 (3) ◽  
pp. 185-189 ◽  
Author(s):  
Adrian Ciurea ◽  
Lukas Hunziker ◽  
Rolf M. Zinkernagel ◽  
Hans Hengartner
Keyword(s):  
Antibody Response ◽  
Neutralizing Antibody ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Viral Escape ◽  
Virus Model

A rapid fluorescent focus-inhibition test for determining dengue neutralizing antibody and for identifying prototype dengue viruses

1978 ◽  
Vol 24 (12) ◽  
pp. 1553-1556 ◽  
Author(s):  
W. Lanier Thacker ◽  
Vester J. Lewis ◽  
George M. Baer ◽  
Gladys E. Sather
Keyword(s):  
Dengue Virus ◽  
Hemagglutination Inhibition ◽  
Neutralizing Antibodies ◽  
Complement Fixation ◽  
Neutralizing Antibody ◽  
Inhibition Test ◽  
Dengue Viruses ◽  
Reduction Test ◽  
Antibody Titers ◽  
Fluorescent Focus

Neutralizing antibody to dengue virus in human and animal sera was assayed by the rapid fluorescent focus-inhibition test (RFFIT). Neutralizing-antibody titers could be detected after only 24 h compared to 5–6 days required by the plaque-reduction test. The RFFIT is more definitive than the conventional complement fixation (CF) or hemagglutination-inhibition (HI) test in identifying prototype dengue viruses, is reproducible, and is applicable to the routine detection of neutralizing antibodies to dengue viruses.

scholarly journals Comparison of three tests for the serological diagnosis of lymphocytic choriomeningitis virus infection

1975 ◽  
Vol 2 (3) ◽  
pp. 193-197
Author(s):  
V J Lewis ◽  
P D Walter ◽  
W L Thacker ◽  
W G Winkler
Keyword(s):  
Complement Fixation ◽  
Neutralization Test ◽  
Fluorescent Antibody ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
The Other ◽  
Virus Antibody ◽  
Infected Cells ◽  
Lymphocytic Choriomeningitis Virus Infection ◽  
High Level

Levels of lymphocytic choriomeningitis virus antibody were assayed in 62 infected persons. The three tests used were indirect fluorescent antibody (IFA), complement fixation, and neutralization in mice. The sera first became positive by the IFA test, and IFA titers rapidly rose to a relatively high level, with the sera remaining positive long after the antibody detectable by complement fixation had disappeared. The IFA test appeared to be specific. The sera became positive last by the mouse neutralization test; with this test, antibody first appeared several weeks after infection. Virus-infected cells were stable when stored at -60 C, allowing diagnostic sera to be tested promptly by the IFA test. The IFA test for lymphocytic choriomeningitis antibody should increase the number of serological diagnoses, since it is not only rapid and specific, but detects cases not diagnosed by the other methods.

scholarly journals Enhanced Virus Clearance by Early Inducible Lymphocytic Choriomeningitis Virus-Neutralizing Antibodies in Immunoglobulin-Transgenic Mice

1998 ◽  
Vol 72 (3) ◽  
pp. 2253-2258 ◽  
Author(s):  
Peter Seiler ◽  
Ulrich Kalinke ◽  
Thomas Rülicke ◽  
Etienne M. Bucher ◽  
Christian Böse ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Antibody Response ◽  
Acute Phase ◽  
Neutralizing Antibodies ◽  
Neutralizing Antibody ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Immunoglobulin M ◽  
Neutralizing Monoclonal Antibody ◽  
Lcmv Infection

ABSTRACT Following infection of mice with lymphocytic choriomeningitis virus (LCMV), virus-neutralizing antibodies appear late, after 30 to 60 days. Such neutralizing antibodies play an important role in protection against reinfection. To analyze whether a neutralizing antibody response which developed earlier could contribute to LCMV clearance during the acute phase of infection, we generated transgenic mice expressing LCMV-neutralizing antibodies. Transgenic mice expressing the immunoglobulin μ heavy chain of the LCMV-neutralizing monoclonal antibody KL25 (H25 transgenic mice) mounted LCMV-neutralizing immunoglobulin M (IgM) serum titers within 8 days after infection. This early inducible LCMV-neutralizing antibody response significantly improved the host’s capacity to clear the infection and did not cause an enhancement of disease after intracerebral (i.c.) LCMV infection. In contrast, mice which had been passively administered LCMV-neutralizing antibodies and transgenic mice exhibiting spontaneous LCMV-neutralizing IgM serum titers (HL25 transgenic mice expressing the immunoglobulin μ heavy and the κ light chain) showed an enhancement of disease after i.c. LCMV infection. Thus, early-inducible LCMV-neutralizing antibodies can contribute to viral clearance in the acute phase of the infection and do not cause antibody-dependent enhancement of disease.

OUTBREAK OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS INFECTIONS IN MEDICAL CENTER PERSONNEL1

1975 ◽  
Vol 101 (2) ◽  
pp. 103-110 ◽  
Author(s):  
ALAN R. HINMAN ◽  
DAVID W. FRASER ◽  
R. GORDON DOUGLAS ◽  
G. STEPHEN BOWEN ◽  
ALAN L. KRAUS ◽  
...  
Keyword(s):  
Medical Center ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Virus Infections
Sign in / Sign up

Export Citation Format

Share Document