Viral escape from the neutralizing antibody response: the lymphocytic choriomeningitis virus model

2001 ◽  
Vol 53 (3) ◽  
pp. 185-189 ◽  
Author(s):  
Adrian Ciurea ◽  
Lukas Hunziker ◽  
Rolf M. Zinkernagel ◽  
Hans Hengartner
Keyword(s):  
Antibody Response ◽  
Neutralizing Antibody ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Viral Escape ◽  
Virus Model

A rapid fluorescent focus-inhibition test for determining the neutralizing-antibody response to lymphocytic choriomeningitis virus

1977 ◽  
Vol 23 (5) ◽  
pp. 522-526 ◽  
Author(s):  
W. Lanier Thacker ◽  
Vester J. Lewis ◽  
Gary J. Haller ◽  
George M. Baer
Keyword(s):  
Antibody Response ◽  
Complement Fixation ◽  
Fluorescent Antibody ◽  
Neutralizing Antibody ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Inhibition Test ◽  
Indirect Fluorescent Antibody ◽  
Virus Infections ◽  
Fluorescent Focus

Levels of neutralizing antibody to lymphocytic choriomeningitis (LCM) virus in the sera of 66 infected persons were assayed by a rapid fluorescent focus-inhibition test (RFFIT). The test was more sensitive than the mouse-neutralization (MN) test and could be completed in less than 24 h. The RFFIT titers were compared with titers obtained by the indirect fluorescent-antibody (IFA) and complement-fixation (CF) tests. Neutralizing antibody detected by the RFFIT remained positive after IFA, CF, and MN antibodies had disappeared. The RFFIT for detection of LCM antibody is specific and reproducible and seems especially useful for determining the incidence and epidemiology of LCM virus infections.

scholarly journals Enhanced Virus Clearance by Early Inducible Lymphocytic Choriomeningitis Virus-Neutralizing Antibodies in Immunoglobulin-Transgenic Mice

1998 ◽  
Vol 72 (3) ◽  
pp. 2253-2258 ◽  
Author(s):  
Peter Seiler ◽  
Ulrich Kalinke ◽  
Thomas Rülicke ◽  
Etienne M. Bucher ◽  
Christian Böse ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Antibody Response ◽  
Acute Phase ◽  
Neutralizing Antibodies ◽  
Neutralizing Antibody ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Immunoglobulin M ◽  
Neutralizing Monoclonal Antibody ◽  
Lcmv Infection

ABSTRACT Following infection of mice with lymphocytic choriomeningitis virus (LCMV), virus-neutralizing antibodies appear late, after 30 to 60 days. Such neutralizing antibodies play an important role in protection against reinfection. To analyze whether a neutralizing antibody response which developed earlier could contribute to LCMV clearance during the acute phase of infection, we generated transgenic mice expressing LCMV-neutralizing antibodies. Transgenic mice expressing the immunoglobulin μ heavy chain of the LCMV-neutralizing monoclonal antibody KL25 (H25 transgenic mice) mounted LCMV-neutralizing immunoglobulin M (IgM) serum titers within 8 days after infection. This early inducible LCMV-neutralizing antibody response significantly improved the host’s capacity to clear the infection and did not cause an enhancement of disease after intracerebral (i.c.) LCMV infection. In contrast, mice which had been passively administered LCMV-neutralizing antibodies and transgenic mice exhibiting spontaneous LCMV-neutralizing IgM serum titers (HL25 transgenic mice expressing the immunoglobulin μ heavy and the κ light chain) showed an enhancement of disease after i.c. LCMV infection. Thus, early-inducible LCMV-neutralizing antibodies can contribute to viral clearance in the acute phase of the infection and do not cause antibody-dependent enhancement of disease.

scholarly journals Viruses Teaching Immunology: Role of LCMV Model and Human Viral Infections in Immunological Discoveries

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 106 ◽  
Author(s):  
Mohamed Abdel-Hakeem
Keyword(s):  
Major Histocompatibility Complex ◽  
Immune System ◽  
Essential Role ◽  
Viral Infections ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Viral Escape ◽  
Escape Mutation ◽  
Histocompatibility Complex

Virology has played an essential role in deciphering many immunological phenomena, thus shaping our current understanding of the immune system. Animal models of viral infection and human viral infections were both important tools for immunological discoveries. This review discusses two immunological breakthroughs originally identified with the help of the lymphocytic choriomeningitis virus (LCMV) model; immunological restriction by major histocompatibility complex and immunotherapy using checkpoint blockade. In addition, we discuss related discoveries such as development of tetramers, viral escape mutation, and the phenomenon of T-cell exhaustion.

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