Adsorption of Pseudomonas pseudomallei cytophilic antibodies onto rabbit alveolar macrophages

Richard A. Kishimoto; Warren C. Eveland

doi:10.1139/m76-230

Adsorption of Pseudomonas pseudomallei cytophilic antibodies onto rabbit alveolar macrophages

Canadian Journal of Microbiology ◽

10.1139/m76-230 ◽

1976 ◽

Vol 22 (10) ◽

pp. 1577-1579

Author(s):

Richard A. Kishimoto ◽

Warren C. Eveland

Keyword(s):

Alveolar Macrophages ◽

Rosette Formation ◽

Pseudomonas Pseudomallei ◽

Cytophilic Antibody

Cytophilic antibody has been demonstrated in sera from rabbits immunized with Pseudomonas pseudomallei. This antibody passively adsorbed onto normal rabbit alveolar macrophages rendered the macrophages agglutinable by P. pseudomallei antigens and capable of displaying 'rosette' formation with polysaccharide-sensitized erythrocytes.

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The effects of lead ion on immune function of rabbit alveolar macrophages: Quantitation of immune phagocytosis and rosette formation by 51Cr in vitro

Toxicology and Applied Pharmacology ◽

10.1016/0041-008x(85)90256-x ◽

1985 ◽

Vol 78 (3) ◽

pp. 484-487 ◽

Cited By ~ 8

Author(s):

Zhou Jian ◽

Xu Ying-Han ◽

Chang Hong-Fu

Keyword(s):

Immune Function ◽

Alveolar Macrophages ◽

Rosette Formation ◽

Lead Ion

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Induction of microbial variants of Pseudomonas pseudomallei in cultured rabbit alveolar macrophages

Canadian Journal of Microbiology ◽

10.1139/m75-303 ◽

1975 ◽

Vol 21 (12) ◽

pp. 2112-2115 ◽

Cited By ~ 2

Author(s):

Richard A. Kishimoto ◽

Warren C. Eveland

Keyword(s):

Alveolar Macrophages ◽

Peritoneal Macrophages ◽

Pseudomonas Pseudomallei

Cultured alveolar and peritoneal macrophages obtained from normal and immunized rabbits were used to induce wall-defective microbial variants of Pseudomonas pseudomallei. Variants were induced only by normal alveolar macrophages. The variants reverted to typical Pseudomonas forms either spontaneously or upon transfer into broth or agar.

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Quantification of Silica Uptake by Alveolar Macrophages - An Emperical Scanning Electron Microprobe Method

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100054297 ◽

1982 ◽

Vol 40 ◽

pp. 332-333

Author(s):

V. V. Damiano ◽

R. P. Daniele ◽

H. T. Tucker ◽

J. H. Dauber

Keyword(s):

Quantitative Analysis ◽

Alveolar Macrophages ◽

Bulk Sample ◽

Silica Particles ◽

Empirical Method ◽

Phagocytic Cells ◽

Calibration Standards ◽

X Ray ◽

Accurate Quantitative Analysis ◽

Scanning Electron

An important example of intracellular particles is encountered in silicosis where alveolar macrophages ingest inspired silica particles. The quantitation of the silica uptake by these cells may be a potentially useful method for monitoring silica exposure. Accurate quantitative analysis of ingested silica by phagocytic cells is difficult because the particles are frequently small, irregularly shaped and cannot be visualized within the cells. Semiquantitative methods which make use of particles of known size, shape and composition as calibration standards may be the most direct and simplest approach to undertake. The present paper describes an empirical method in which glass microspheres were used as a model to show how the ratio of the silicon Kα peak X-ray intensity from the microspheres to that of a bulk sample of the same composition correlated to the mass of the microsphere contained within the cell. Irregular shaped silica particles were also analyzed and a calibration curve was generated from these data.

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