Diagnosis of rubella virus infections. II. A comparison between incident and transmitted light fluorescence for the detection of specific IgM antibodies

1975 ◽  
Vol 21 (3) ◽  
pp. 417-418 ◽  
Author(s):  
Pierre Payment ◽  
Lucille Roy ◽  
Jean-Claude Gilker ◽  
André Chagnon
Keyword(s):  
Rubella Virus ◽  
Incident Light ◽  
Transmitted Light ◽  
Virus Infections ◽  
Igm Antibodies ◽  
Light System ◽  
Examination Time ◽  
Light Fluorescence

The fluorescence obtained with an incident light system is higher than that obtained with a transmitted light system. Examination time is reduced and the strain on the eyes, after long periods, is reduced. However, the results are the same whatever the system used.

Diagnosis of rubella virus infections. I. A comparison between Vero and BHK-21 cells for the detection of specific IgG and IgM antibodies by immunofluorescence

1975 ◽  
Vol 21 (3) ◽  
pp. 289-292 ◽  
Author(s):  
Pierre Payment ◽  
Lucille Roy ◽  
Jean-Claude Gilker ◽  
André Chagnon
Keyword(s):  
Rubella Virus ◽  
Vero Cells ◽  
Virus Infections ◽  
Igm Antibodies ◽  
Specific Igg

Vero cells were more suitable then BHK-21 cells for the detection of rubella-specific 1gG antibodies because the nonspecific fluorescence was minimal. However, BHK-21 cells were found more sensitive than Vero cells for the detection of rubella-specific IgM antibodies.

CAROTID ARTERY THROMBOSIS, ENCEPHALITIS, MYELITIS AND OPTIC NEURITIS ASSOCIATED WITH RUBELLA VIRUS INFECTIONS

Brain ◽  
1975 ◽  
Vol 98 (4) ◽  
pp. 583-594 ◽  
Author(s):  
J. H. CONNOLLY ◽  
W. M. HUTCHINSON ◽  
INGRID V. ALLEN ◽  
J. A. LYTTLE ◽  
M. W. SWALLOW ◽  
...  
Keyword(s):  
Carotid Artery ◽  
Optic Neuritis ◽  
Rubella Virus ◽  
Virus Infections ◽  
Carotid Artery Thrombosis ◽  
Artery Thrombosis

scholarly journals Development of a Rapid and Convenient Method for Determination of Rubella Virus-Specific Immunoglobulin G Avidity

2007 ◽  
Vol 14 (11) ◽  
pp. 1416-1419 ◽  
Author(s):  
Christelle Vauloup-Fellous ◽  
Jessica Ursulet-Diser ◽  
Liliane Grangeot-Keros
Keyword(s):  
Immunoglobulin G ◽  
Rubella Virus ◽  
Primary Infection ◽  
Virus Infections ◽  
Serum Samples ◽  
Igg Avidity ◽  
Specific Immunoglobulin ◽  
Specific Igg ◽  
Semiautomated Method

ABSTRACT We describe here a rapid and semiautomated method for the determination of rubella virus immunoglobulin G (IgG) avidity with the VIDAS instrument. A total of 153 serum samples from persons with naturally acquired rubella virus infections (n = 98), from vaccinated persons (n = 44), and from patients with autoantibodies (n = 11) were included in this study. The rubella virus-specific IgG avidity assay we developed for the VIDAS instrument was evaluated by comparison with an in-house method. Results obtained with the VIDAS instrument allow considering this method valuable to help confirm or exclude acute primary infection or recent vaccination.

scholarly journals Cytokine changes in the aqueous humor in rubella-related Fuchs heterochromic iridocyclitis

2021 ◽  
Author(s):  
Shangkun ou ◽  
Yi Mao ◽  
Sijie Lin ◽  
Chengfang Zhu ◽  
Xiaodong Liu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Aqueous Humor ◽  
Rubella Virus ◽  
Negative Group ◽  
Virus Infections ◽  
Related Factors ◽  
Adhesion Protein ◽  
Positive Group ◽  
Significant Difference ◽  
Simplex Virus

Abstract This retrospective study aimed to determine the correlation between cytokine levels and virus status in the aqueous humor of 38 patients with Fuchs heterochromic iridocyclitis (FHI) with/without viral presence between May 2017 and January 2020. The levels of cytokines were analyzed in the groups with and without virus-related FHI. Among the patients, 50% had rubella virus, 5.26% had cytomegalovirus, and 2.63% had herpes simplex virus infections. The expression of interleukin-6 (IL-6) and IL-8 was significantly higher and that of basic fibroblast growth factor (bFGF) was significantly lower in the virus-positive group than in the virus-negative group (P = 0.015, P = 0.001, and P = 0.001, respectively). Although there was no significant difference in the mean expression of vascular cell adhesion protein 1 (VCAM-1), IL-10, and vascular endothelial growth factor (VEGF), those of VCAM-1 and IL-10 were higher (M = 1338, M = 1390, respectively; M = 6.225 and 10.600, respectively) and that of VEGF was lower (M = 134.5, M = 38.70, respectively) in the virus-positive group than in the virus-negative group. Similar findings were observed for the expressions of IL-6, IL-8, and bFGF in the rubella-positive and -negative groups. Viral presence was highly related to FHI, especially that of the rubella virus. High levels of inflammatory cytokines and low levels of neovascularization-related factors are involved in rubella-related FHI. These study findings could be helpful in the diagnosis and treatment of FHI.

scholarly journals Seroprevalence Of Rubella Virus Antibodies Among Pregnant Women Attending Antenatal Clinic in Ekiti State

2021 ◽  
Vol 3 (3) ◽  
pp. 18-21
Author(s):  
O. Ojo Bola ◽  
T. O. Korode ◽  
D. E. Oguntunnbi ◽  
F. B. Ajimojuowo ◽  
A. A. Aladejare ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Rubella Virus ◽  
Tertiary Education ◽  
Post Partum ◽  
Demographic Data ◽  
Occupational Status ◽  
Antenatal Clinic ◽  
Igm Antibodies ◽  
Congenital Rubella ◽  
Rubella Virus Infection

Rubella virus infection poses a great threat to the foetus whose mother acquires the infection. This study was therefore carried out to determine the seroprevalence of rubella virus IgM antibodies among the pregnant women attending Federal Teaching Hopsital, Ido Ekiti. One hundred and ninety two (192) sera were collected from pregnant women and screened for rubella virus IgM antibodies. A structured questionnaire was administered to subjects to obtain socio-demographic data. The sera samples were analysed using Enzymes Immunosorbent Assay (EIA) IgM rubella kit. Out of 192 pregnant women screened, 6(3.1%) subjects were sero-positive. Age group 31-35years recorded the highest prevalence 3(1.56%). Pregnant women with tertiary education had the highest prevalence of 4(2.08%) among different educational level; civil servants have a prevalence of 4(2.08%) compared with other occupational status. However, prenatal screening and post-partum is highly encouraged to detect congenital rubella syndrome. There is also a need to include rubella screening as part of the routine procedure for the expectant mother.

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