In vitro measurement of cytotoxic antibodies in mouse-immune sera against mouse ascites tumor cells

1974 ◽  
Vol 20 (12) ◽  
pp. 1681-1688 ◽  
Author(s):  
C. P. Eng ◽  
W. R. Tolbert ◽  
J. B. Harnaha ◽  
J. P. Concannon
Keyword(s):  
Tissue Culture ◽  
Tumor Cells ◽  
Specific Antigen ◽  
Rabbit Serum ◽  
In Vitro Test ◽  
Ascites Tumor ◽  
Serum Samples ◽  
Culture Cells ◽  
Tissue Culture Cells

Previous studies reported that nontumorigenic 6C3HED tissue-culture cells induced strong tumor-specific immunoprotection against 6C3HED tumors. However, in the in vitro test, sera from C3H mice immunized with nontumorigenic 6C3HED tissue-culture cells failed to lyse 6C3HED ascites tumor cells when fresh guinea pig serum was incorporated as the complement source. A number of serum samples from different animal species were assayed as a complement source. With the exception of fresh rabbit serum, all other sera were either by themselves toxic to 6C3HED ascites tumor cells, or did not function as a complement source. The rabbit serum, by itself, was not toxic to 6C3HED tumor cells, but when incorporated with the isogeneic mouse-immune serum, killed more than 95% tumor cells. The facilitating activity exhibited by rabbit serum was characterized as classical complement and was not due to the presence of heterophile antibodies in the rabbit serum. By using this immunolytic testing system, the sera from mice bearing 6C3HED, S-180, TA-3, and Ehrlich solid tumors for an extensive time were found toxic to homologous ascites tumor cells. The cross-reactivity of the sera from tumor-bearing animals suggests that these four murine tumors, 6C3HED, S-180, TA-3, and Ehrlich, share some common tumor-specific antigen(s).

Starch-enhanced synthesis and release of amylolytic enzymes from normal and crown gall tumor tobacco tissue culture cells

1982 ◽  
Vol 60 (8) ◽  
pp. 1474-1478 ◽  
Author(s):  
Charles Voliva ◽  
Gustave W. Moessen ◽  
Ann G. Matthysse
Keyword(s):  
Tissue Culture ◽  
Carbon Source ◽  
Tumor Cells ◽  
Crown Gall ◽  
Amylolytic Activity ◽  
Amylolytic Enzymes ◽  
Crown Gall Tumor ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Tobacco Tissue Culture

The response of tobacco crown gall tumor cells growing in tissue culture to a substitution of starch for sucrose as the carbon source in the medium was compared with the response of normal tobacco tissue culture cells. In both cases amylolytic activity was secreted into the medium. The increase in extracellular amylolytic activity was preceded by an increase in intracellular activity. The increase in intracellular amylolytic enzymes was sensitive to cycloheximide and to actinomycin D indicating that enzyme induction at the level of mRNA synthesis was required. No significant difference between the responses of normal and tumor cells was observed. Thus crown gall tumor cells were as capable as normal cells of sensing and responding to an alteration in the carbon source in the external medium.

scholarly journals Cultivation of Pathogenic and Opportunistic Free-Living Amebas

2002 ◽  
Vol 15 (3) ◽  
pp. 342-354 ◽  
Author(s):  
Frederick L. Schuster
Keyword(s):  
Tissue Culture ◽  
Clinical Samples ◽  
Human Pathogens ◽  
Free Living ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Feeder Layers ◽  
Important Means ◽  
Human Infections

SUMMARY Free-living amebas are widely distributed in soil and water, particularly members of the genera Acanthamoeba and Naegleria. Since the early 1960s, they have been recognized as opportunistic human pathogens, capable of causing infections of the central nervous system (CNS) in both immunocompetent and immunocompromised hosts. Naegleria is the causal agent of a fulminant CNS condition, primary amebic meningoencephalitis; Acanthamoeba is responsible for a more chronic and insidious infection of the CNS termed granulomatous amebic encephalitis, as well as amebic keratitis. Balamuthia sp. has been recognized in the past decade as another ameba implicated in CNS infections. Cultivation of these organisms in vitro provides the basis for a better understanding of the biology of these amebas, as well as an important means of isolating and identifying them from clinical samples. Naegleria and Acanthamoeba can be cultured axenically in cell-free media or on tissue culture cells as feeder layers and in cultures with bacteria as a food source. Balamuthia, which has yet to be isolated from the environment, will not grow on bacteria. Instead, it requires tissue culture cells as feeder layers or an enriched cell-free medium. The recent identification of another ameba, Sappinia diploidea, suggests that other free-living forms may also be involved as causal agents of human infections.

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