Removal of resin acids from culture media and kraft-mill effluents by yeasts and some other fungi

1974 ◽  
Vol 20 (9) ◽  
pp. 1288-1290 ◽  
Author(s):  
J. F. T. Spencer ◽  
G. D. Sinclair ◽  
N. R. Gardner
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acid Content ◽  
Culture Media ◽  
Mutagenic Activity ◽  
Resin Acid ◽  
Resin Acids ◽  
Trichosporon Cutaneum ◽  
Sole Nitrogen Source ◽  
Kraft Mill Effluent ◽  
Kraft Mill

Epicoccum nigrum, Rhinocladiella mansonii, Rhodotorula minuta, and Trichosporon cutaneum were tested for their ability to remove resin acids from supplemented kraft-mill effluent and from a medium containing yeast extract as sole nitrogen source. Up to 39% of the resin acid content was removed by all four organisms. The mutagenic activity of the resin acid preparation, as measured by the induction of gene conversion in a strain of Saccharomyces cerevisiae, was low or non-existent.

scholarly journals Karakteristik Asam Resin Kopal Agathis loranthifolia Sukabumi (Characteristic Resin Acid of Kopal Agathis loranthifolia Sukabumi)

1970 ◽  
Vol 4 (2) ◽  
pp. 13-17
Author(s):  
Ika Resmeiliana ◽  
Kurnia Sofyan ◽  
Suminar S. Achmadi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Acid Content ◽  
Raw Materials ◽  
Resin Acid ◽  
Resin Acids ◽  
Gas Chromatography Mass Spectrometry ◽  
Alpha Pinene ◽  
Limonene Oxide

Copal is originated from the exudate of Agathis loranthifolia tree, which flows out from tapping. copal is one of Indonesia's export comodities. Unfortunately, since the export was still in the form of raw materials, it was necessary to study the identification of resin acid content of the copal of Sukabumi. It was also necessary to test the quality of the copal. Extraction was done by using acetone: MeOH (9:1) solvent. Then the extraction of resin acid content was identified by using Gas Chromatography Mass Spectrometry (CGMS). The quality of copal was evaluated based on SNI 7634-2011. Resin acids of the copal which were identified by CGMS are limonene (44%), ethylene oxide hexamer, cis-limonene oxide, toluene, trans-carveol, 2-siclohexane-1-one, trans-limonene oxide, and alpha pinene. Copal of Sukabumi belong to the first class, because the obtained parameters are not all included in the premier class ranges. However it is still possible to be upgraded to their premier class by removing the dirt.Keywords : Agathis loranthifolia, copal, limonene, resin acids.

The Occurrence and Bioavailability of Retene and Resin Acids in Sediments of a Lake Receiving BKME (Bleached Kraft Mill Effluent)

1999 ◽  
Vol 40 (11-12) ◽  
pp. 131-138 ◽  
Author(s):  
H. J. T. Leppänen ◽  
A. O. J. Oikari
Keyword(s):  
Activated Sludge ◽  
Waste Water Treatment ◽  
Paper Mill ◽  
Pulp And Paper ◽  
Resin Acids ◽  
Background Concentration ◽  
Pulp And Paper Mill ◽  
Paper Mill Effluents ◽  
Kraft Mill Effluent ◽  
Kraft Mill

Retene (7-isopropyl-1-methylphenantrene) is an alkyl substituted PAH derived primarily from bacterial aromatization of abietic type resin acids. Retene has been shown to induce cytochrome P450 1A in rainbow trout whereas e.g. dehydroabietic acid does not induce it. We analysed resin acids and retene in sediments from seven sites receiving pulp and paper mill effluents from 4 mills, and from two reference sites. All mills have employed treatment of waste waters by activated sludge. The highest concentration of retene measured in sediment was 1600 μg/g d.w. (11 700 μg/g organic carbon, OC) while the highest concentration of resin acids was 1500 μg/g d.w. (9 300 μg/g OC). Downstream from the point (12 km) of bleached kraft mill effluent discharge, the concentration of retene at the depth of 5-10 cm in sediment was still 16 μg/g d.w. (650 μg/g OC) and the concentration of resin acids 139 μg/g d.w. (1700 μg/g OC). Background concentration for retene on the upstream reference site was below 0.1 μg/g d.w and for resin acids below 70 μg/g d.w. Substantial concentrations of retene (54 μg/g d.w.) and resin acids (1470 μg/g d.w.) were also detected in sedimenting particles collected at the sediment sampling sites. When the concentrations and sedimentation rates before and after the installation of activated sludge systems were compared, the effect of improved waste water treatment on total amount of sedimenting resin acids and retene (μg/m2/day) is clear. To assess the bioavailability of retene we analysed its concentration in the bile of feral fish caught 1-2 km downstream of pulp and paper mill. Data on roach shows that retene in sediments can be bioavailable to fish feeding on benthic food chain.

Resin acid variation in sun and shade needles of Scots pine (Pinussylvestris L.)

1987 ◽  
Vol 17 (4) ◽  
pp. 346-349 ◽  
Author(s):  
Rolf Gref ◽  
Olle Tenow
Keyword(s):  
Chemical Composition ◽  
Genetic Markers ◽  
Acid Content ◽  
Resin Acid ◽  
Resin Acids ◽  
Northern Sweden ◽  
Southern Sweden ◽  
Sun And Shade Needles ◽  
Sun And Shade

Bioclimatic and physiological differences between sun and shade needles suggest that their chemical composition should differ. An analysis of resin acid content demonstrated that although the same resin acids were found (i.e., levopimaric/palustric, dehydroabietic, abietic, neoabietic, 4-epiimbricatolic, pinifolic, and isopimaric acids), both individual and total resin concentrations were significantly higher in sun than in shade needles. These relationships were true for a dense 40-year-old pine stand in southern Sweden as well as for a dense 12-year-old stand in northern Sweden. The role of resin acids in defence against needle-eating insects and the relative susceptibilities of shade and sun needles to needle-attacking organisms are discussed. Variation in resin acid concentration in sun and shade needles should be considered when such acids are used as genetic markers.

Responses in a Fathead Minnow (Pimephales promelas) Lifecycle Test and in Wild White Sucker (Catostomus commersoni) Exposed to a Canadian Bleached Kraft Mill Effluent

2010 ◽  
Vol 45 (2) ◽  
pp. 187-200 ◽  
Author(s):  
Joanne L. Parrott ◽  
L. Mark Hewitt ◽  
Tibor G. Kovacs ◽  
Deborah L. MacLatchy ◽  
Pierre H. Martel ◽  
...  
Keyword(s):  
Egg Production ◽  
Fathead Minnow ◽  
Pimephales Promelas ◽  
Fish Reproduction ◽  
White Sucker ◽  
Catostomus Commersoni ◽  
Liver Size ◽  
Paper Mill Effluent ◽  
Kraft Mill Effluent ◽  
Kraft Mill

Abstract To evaluate currently available bioassays for their use in investigating the causes of pulp and paper mill effluent effects on fish reproduction, the responses of wild white sucker (Catostomus commersoni) collected from the receiving environment at the bleached kraft mill at La Tuque, Quebec, were compared with responses of fathead minnow (Pimephales promelas) exposed to effluent in a laboratory lifecycle test. White sucker collected at effluent exposed sites had increased liver size but none of the reproductive effects that had been documented in earlier field studies at this site. Exposure to 1, 3, 10, 30, and 100% bleached kraft mill effluent (BKME) in the lab led to significantly decreased length, but increased weight and liver size in male fathead minnow. Female length was also decreased and liver size was increased at high effluent exposures. Most effluent concentrations (1 to 30%) significantly increased egg production compared with controls. The fathead minnow lifecycle assay mirrored the effects seen in wild fish captured downstream of the BKME discharge. These results will be used to select short-term fish tests for investigating the causes of and solutions to the effects of mill effluents on fish reproduction.

Mutagenicity in Pulp and Paper Mill Effluents and in Recipient

1988 ◽  
Vol 20 (2) ◽  
pp. 143-152 ◽  
Author(s):  
A. Langi ◽  
M. Priha
Keyword(s):  
Activated Sludge ◽  
Mutagenic Activity ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Paper Mill ◽  
Pulp And Paper ◽  
Pulp And Paper Mill ◽  
Sludge Treatment ◽  
Paper Mill Effluents ◽  
Kraft Mill

The mutagenic properties of pulp and paper mill effluents were studied in three mills: bleached kraft mill with aerated lagoon treatment (Mill 1), bleached kraft mill with activated sludge treatment (Mill 2) and mechanical pulp/paper mill (Mill 3). Both treated and untreated effluents, process streams and molecular fractions were tested for mutagenicity (Ames test. Salmonella typhimurium TA100 and SCE sister chromatid exchange test, Chinese hamster ovary cells). To verify the potential environmental effects the mutagenic activity of concentrated recipient lake water (Mill 2) was also studied. The Ames mutagenicity of the bleached kraft mill effluent (BKME) originated from the first chlorination filtrate, SCE mutagenicity also occurred in the alkali extraction stage filtrate (Mill 1). No Ames mutagenicity was detected in the paper mill effluent, but it was SCE mutagenic. Activated sludge treatment of BKME removed both Ames and SCE mutagenicity, but the aerated lagoon treated BKME was still SCE mutagenic. No mutagenic activity was detected in the recipient water concentrates.

Cytochrome P450 mono-oxygenases in conifer genomes: discovery of members of the terpenoid oxygenase superfamily in spruce and pine

2006 ◽  
Vol 34 (6) ◽  
pp. 1209-1214 ◽  
Author(s):  
B. Hamberger ◽  
J. Bohlmann
Keyword(s):  
Cytochrome P450 ◽  
Loblolly Pine ◽  
Large Scale ◽  
Expressed Sequence Tag ◽  
Resin Acid ◽  
Functional Characterization ◽  
Structural Diversity ◽  
Resin Acids ◽  
Full Length Sequence ◽  
Diterpene Resin Acids

Diterpene resin acids, together with monoterpenes and sesquiterpenes, are the most prominent defence chemicals in conifers. These compounds belong to the large group of structurally diverse terpenoids formed by enzymes known as terpenoid synthases. CYPs (cytochrome P450-dependent mono-oxygenases) can further increase the structural diversity of these terpenoids. While most terpenoids are characterized as specialized or secondary metabolites, some terpenoids, such as the phytohormones GA (gibberellic acid), BRs (brassinosteroids) and ABA (abscisic acid), have essential functions in plant growth and development. To date, very few CYP genes involved in conifer terpenoid metabolism have been functionally characterized and were limited to two systems, yew (Taxus) and loblolly pine (Pinus taeda). The characterized yew CYP genes are involved in taxol diterpene biosynthesis, while the only characterized pine terpenoid CYP gene is part of DRA (diterpene resin acid) biosynthesis. These CYPs from yew and pine are members of two apparently conifer-specific CYP families within the larger CYP85 clan, one of four plant CYP multifamily clans. Other CYP families within the CYP85 clan were characterized from a variety of angiosperms with functions in terpenoid phytohormone metabolism of GA, BR, and ABA. The recent development of EST (expressed sequence tag) and FLcDNA (where FL is full-length) sequence databases and cDNA collections for species of two conifers, spruce (Picea) and pine, allows for the discovery of new terpenoid CYPs in gymnosperms by means of large-scale sequence mining, phylogenetic analysis and functional characterization. Here, we present a snapshot of conifer CYP data mining, discovery of new conifer CYPs in all but one family within the CYP85 clan, and suggestions for their functional characterization. This paper will focus on the discovery of conifer CYPs associated with diterpene metabolism and CYP with possible functions in the formation of GA, BR, and ABA in conifers.

scholarly journals Electromagnetic Fields (0.04 to 0.39) mT effect on cellular growth cycles of Saccharomyces cerevisiae wine strains

2019 ◽  
Vol 17 (2) ◽  
pp. 196
Author(s):  
Eliseo Amado-González ◽  
Alveiro Álvarez Ovallos ◽  
Alfonso Quijano Parra
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Electromagnetic Fields ◽  
Biomass Production ◽  
Exposure Time ◽  
Culture Media ◽  
Dry Weight ◽  
Cellular Growth ◽  
Biomass Growth ◽  
Yeast Saccharomyces Cerevisiae ◽  
Growth Cycles

Low frecuency electromagnetic fields effect (EMF) on growth cycles of yeast Saccharomyces cerevisiae wine strains Rv1 and Rhône were studied.  A cylindrical coil induced magnetic fields with inductions up to 0,39 mT. Exposure time to EMF varied between (1 – 10) min at 30 °C.  The biomass growth were monitored in the reactor culture media (yeast extract + by measurement optical density from (0 to 32) h. The biomass was found by dry weight. After yeast expose to the different EMF, the number of growth cycles decreased from 4 cycles to 2 or 1. However, the biomass production increased almost 50 %.  The best biomass production was found at 0.39 mT and 10 min exposure time.  Keywords: Electromagnetic fields, Saccharomyces cerevisiae, biomass production, RV1

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