Large-scale production of staphylococcal delta hemolysin by the dialysis membrane technique

1974 ◽  
Vol 20 (7) ◽  
pp. 1061-1063 ◽  
Author(s):  
Richard A. Murphy ◽  
Riaz-Ul Haque
Keyword(s):  
Large Scale ◽  
Culture Medium ◽  
Scale Production ◽  
Dialysis Membrane ◽  
Large Scale Production ◽  
Membrane Technique

A procedure has been developed which uses the dialysis membrane technique for producing and rapidly harvesting staphylococcal delta hemolysin from several hundred plates of culture medium.

scholarly journals Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

Revista CERES ◽  
2013 ◽  
Vol 60 (2) ◽  
pp. 152-160 ◽  
Author(s):  
Leticia Mascarenhas Pereira Barbosa ◽  
Vespasiano Borges de Paiva Neto ◽  
Leonardo Lucas Carnevalli Dias ◽  
Reginaldo Alves Festucci-Buselli ◽  
Rodrigo Sobreira Alexandre ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Culture Medium ◽  
Shoot Proliferation ◽  
Cellular Level ◽  
Fragaria X Ananassa ◽  
Scale Production ◽  
Ms Medium ◽  
Large Scale Production

In vitro propagation has become an effective practice for large-scale production of strawberry plants. The objective of this study was to evaluate the hyperhydricity and the multiplication capacity of two strawberry varieties (Fragaria x ananassa Duch. 'Dover' and 'Burkley') propagated in vitro. Plants maintained in MS medium supplemented with 1.0 mg L-1 BA were individualized and transferred to the same medium solidified with Agar (6.5 g L-1) or Phytagel® (2.5 g L-1) and BA at different concentrations (0; 0.5; 1.0; 2.0 and 3.0 mg L-1). Biochemical and anatomical analyses were carried out, as well as the analysis of the morphological hyperhydricity characteristics. The analysis of data showed: a) the increase in cytokinin concentration increased hyperhydricity frequency in both varieties; b) at concentrations up to 2.0 mg L-1 BA, the replacement of Agar by Phytagel® induced a higher formation of hyperhydric shoots; and c) the addition of BA induced oxidative stress, which is characterized by increased antioxidant activity and lipid peroxidation, as well as alterations at the cellular level, such as malformation of stomata and epidermal cells. In conclusion, the culture medium containing 0.5 mg L-1 BA solidified with Agar provided lower hyperhydricity percentages in association with higher rates of shoot proliferation in strawberry.

scholarly journals A Novel Approach Using Conventional Methodologies to Scale up BNC Production Using Komagataeibacter medellinensis and Rotten Banana Waste as Alternative

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1469
Author(s):  
Carlos Molina-Ramírez ◽  
Juan Álvarez ◽  
Robin Zuluaga ◽  
Cristina Castro ◽  
Piedad Gañán
Keyword(s):  
Production Process ◽  
Large Scale ◽  
Culture Medium ◽  
Scale Up ◽  
Glucose Consumption ◽  
Human Consumption ◽  
Scale Production ◽  
Bacterial Nanocellulose ◽  
Large Scale Production ◽  
Glucose Diffusion

Currently, cellulose nanostructures are among the most promising structures, and extensive work in materials and biotechnology industries is aimed at identifying an efficient process of production. Even when production at the laboratory scale is successful, crucial aspects of increased commercial applications for cellulose nanostructures are linked to large-scale production. Large-scale production requires a balance between the cost of the culture medium and product value. Therefore, in this work, for the optimization and scaling up of bacterial nanocellulose, a culture medium consisting of rotten banana unsuitable for human consumption was used for the first time as an inexpensive feedstock. Initially, the bacterial nanocellulose (BNC) culture medium conditions were optimized, and it was established that a glucose concentration of 26.4 g/L and a V/A ratio of 2.2 cm were the optimal conditions for production reaching a BNC yield of 5 g/L, which was 42.4% higher than the best result initially obtained. Finally, the scale-up process was performed, implementing a regime analysis methodology by comparing the characteristic times of the critical mechanisms involved in BNC production, namely, microbial growth, glucose consumption, BNC production, and glucose diffusion into the BNC membrane, as the first approach for this type of BNC production process. The mechanism underlying the BNC production process is glucose diffusion into the BNC membrane (characteristic time, 675.47 h). Thus, the V/A ratio was selected as the scale-up criterion most suitable for producing BNC under static culture conditions, allowing the production of 16 g of BNC after 12 d of fermentation in a plastic bioreactor, which was 3378% higher than that produced in glass vessels. The results obtained in this study may initiate further improvements in BNC commercial production by exploiting different feedstocks.

scholarly journals Adaptation of Mycobacterium smegmatis to an Industrial Scale Medium and Isolation of the Mycobacterial PorinMspA

2013 ◽  
Vol 7 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Sebastian O Wendel ◽  
Ayomi S Perera ◽  
Peter H Pfromm ◽  
Peter Czermak ◽  
Stefan H Bossmann
Keyword(s):  
Growth Medium ◽  
Mycobacterium Smegmatis ◽  
Large Scale ◽  
Culture Medium ◽  
Cell Mass ◽  
Cost Effective ◽  
Scale Production ◽  
Large Scale Production ◽  
Simple Growth ◽  
Effective Growth

The adaptation of the organism to a simple and cost-effective growth medium is mandatory in developing a process for large scale production of the octamericporinMspA, which is isolated fromMycobacterium smegmatis. A fermentation optimization with the minimal nutrients required for growth has been performed. During the fermentation, the iron- and ammonium chloride concentrations in the medium were varied to determine their impact on the observed growth rates and cell mass yields. Common antibiotics to control contamination were eliminated in favor of copper sulfate to reduce costs. MspA has been successfully isolated from the harvestedM. smegmatisusingaqueous nOPOE (n-octyloligooxyethylene) at 65°C. Because of the extraordinary stability of MspA, it is possible to denature and precipitate virtually all other proteins and contaminants by following this approach. To further purify the product, acetone is used for precipitation. Gel electrophoresis confirmed the presence and purity of MspA. A maximum of 840µg (via Bradford assay) of pure MspA per liter of the optimized simple growth medium has been obtained. This is a 40% increase with respect to the previously reported culture medium for MspA.

scholarly journals Matrix-immobilized yeast for large-scale production of recombinant human lactoferrin

MedChemComm ◽  
2015 ◽  
Vol 6 (3) ◽  
pp. 486-491 ◽  
Author(s):  
Chun Loong Ho ◽  
In Young Hwang ◽  
Kathy Loh ◽  
Matthew Wook Chang
Keyword(s):  
Large Scale ◽  
Culture Medium ◽  
Human Lactoferrin ◽  
Scale Production ◽  
Improved Method ◽  
Immobilized Yeast ◽  
Recombinant Human Lactoferrin ◽  
Large Scale Production

An improved method of recombinant human lactoferrin (hLF) expression in rich culture medium is demonstrated using macroporous microencapsulation.

scholarly journals Composição do meio de cultivo para produção de microplantas de caju-de-árvore-do-Cerrado (Anacardium othonianum RIZZ.) - Composition of the cultivation medium for the production of microplants of Cerrado-tree cashew (Anacardium othonianum RIZZ.)

2017 ◽  
Vol 4 (1) ◽  
pp. 01 ◽  
Author(s):  
Ísis Danielle Sousa ◽  
Jackellyne Bruna Sousa ◽  
Flávia Dionísio Pereira ◽  
João Das Graças Santana ◽  
Aurélio Rubio Neto ◽  
...  
Keyword(s):  
Activated Charcoal ◽  
Large Scale ◽  
Culture Medium ◽  
Average Length ◽  
Scale Production ◽  
Cultivation Medium ◽  
Large Scale Production ◽  
Number Of Leaves ◽  
Short Time

RESUMOO Anacardium othonianum Rizz. é uma planta típica de regiões de clima tropical caracterizada pela aparência exótica e aroma agradável. Na busca de diversificar a produção e atividades que proporcione maior rentabilidade, a micropropagação tem sido uma alternativa para produção em grande escala em curto espaço de tempo. Por isso, objetivou-se com este trabalho determinar as melhores condições in vitro para micropropagação dessa espécie, para isso, avaliamos a adição de diferentes concentrações de AIB (Ácido Indolbutírico), sacarose e carvão ativado no meio de cultivo in vitro. Foram utilizadas cinco concentrações de AIB (0; 1; 2; 3; 4 mg L-1) e cinco concentrações de sacarose (0, 15, 30, 45, 60 g L-1) na ausência ou presença de carvão ativado (2 g L-1), em meio WPM 50%. Aos 30 e 60 dias foram feitas avaliações do número de explantes oxidados, comprimento médio e número de folhas por explante. Verificou-se que o meio de cultivo suplementado com carvão ativado e a adição de 4 mg L-1 de AIB, contribuiu para a o crescimento de raízes in vitro da espécie. Enquanto, que o meio de cultivo com 30 g sacarose e presença de carvão ativado proporcionou maior comprimento dos explantes e maior número de folhas.Palavras-chave: Frutífera nativa, cerrado, micropropagação.ABSTRACTThe Anacardium othonianum Rizz. It’s a typical plant of regions of tropical climate characterized by the exotic appearance and pleasant aroma. In the quest to diversify production and activities that provide greatest profitability, micropropagation has been an alternative for large-scale production in short time. Therefore, the objective of this work is to determine the best in vitro results for micropropagation of this species, for this, we evaluated the addition of different concentrations of AIB (Indolbutyric Acid), sucrose and activated charcoal in the in vitro culture medium. Five concentrations of AIB (0, 1, 2, 3, 4 mg L-1) and five sucrose concentrations (0, 15, 30, 45, 60 g L-1) were used in the absence or presence of activated charcoal, in WPM 50% medium. At 30 and 60 days, the number of oxidation, average length and number of leaves per explant were evaluated. It was found that the culture medium supplemented with activated charcoal and an addition of 4 mg L-1 of IBA, contributed to in vitro root growth. While the culture medium with 30 g L-1 sucrose and the presence of activated charcoal provided a longer length of the explants and a larger number of leaves.Key words: Native Fruit, Cerrado, Micropropagation.

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