scholarly journals A Novel Approach Using Conventional Methodologies to Scale up BNC Production Using Komagataeibacter medellinensis and Rotten Banana Waste as Alternative

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1469
Author(s):  
Carlos Molina-Ramírez ◽  
Juan Álvarez ◽  
Robin Zuluaga ◽  
Cristina Castro ◽  
Piedad Gañán
Keyword(s):  
Production Process ◽  
Large Scale ◽  
Culture Medium ◽  
Scale Up ◽  
Glucose Consumption ◽  
Human Consumption ◽  
Scale Production ◽  
Bacterial Nanocellulose ◽  
Large Scale Production ◽  
Glucose Diffusion

Currently, cellulose nanostructures are among the most promising structures, and extensive work in materials and biotechnology industries is aimed at identifying an efficient process of production. Even when production at the laboratory scale is successful, crucial aspects of increased commercial applications for cellulose nanostructures are linked to large-scale production. Large-scale production requires a balance between the cost of the culture medium and product value. Therefore, in this work, for the optimization and scaling up of bacterial nanocellulose, a culture medium consisting of rotten banana unsuitable for human consumption was used for the first time as an inexpensive feedstock. Initially, the bacterial nanocellulose (BNC) culture medium conditions were optimized, and it was established that a glucose concentration of 26.4 g/L and a V/A ratio of 2.2 cm were the optimal conditions for production reaching a BNC yield of 5 g/L, which was 42.4% higher than the best result initially obtained. Finally, the scale-up process was performed, implementing a regime analysis methodology by comparing the characteristic times of the critical mechanisms involved in BNC production, namely, microbial growth, glucose consumption, BNC production, and glucose diffusion into the BNC membrane, as the first approach for this type of BNC production process. The mechanism underlying the BNC production process is glucose diffusion into the BNC membrane (characteristic time, 675.47 h). Thus, the V/A ratio was selected as the scale-up criterion most suitable for producing BNC under static culture conditions, allowing the production of 16 g of BNC after 12 d of fermentation in a plastic bioreactor, which was 3378% higher than that produced in glass vessels. The results obtained in this study may initiate further improvements in BNC commercial production by exploiting different feedstocks.

The Research of Production Process Statistical Steady-State Based on the Hypothesis Test

2011 ◽  
Vol 314-316 ◽  
pp. 2433-2438
Author(s):  
Wei Zhi Wang
Keyword(s):  
Quality Control ◽  
Steady State ◽  
Production Process ◽  
Quantitative Method ◽  
Large Scale ◽  
Hypothesis Test ◽  
Influence Factors ◽  
Normal Operation ◽  
Scale Production ◽  
Large Scale Production

By only applying a after the event exam in the quality control of the batch production is not enough to meet the needs of modern large-scale production. To a certain extent, modern quality control is a dynamic process of the steady-state judge and adjustment. A simple and reliable steady-state judge rule and method is the premise to guarantee the normal operation. This paper provides a quantitative method to evaluate production process steady-state by analyzing influence factors based on mathematical statistics. The method is both suitable for simple production process and complex production process with sub-processes.

scholarly journals Foaming of rhamnolipids fermentation: impact factors and fermentation strategies

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Zhijin Gong ◽  
Ge Yang ◽  
Chengchuan Che ◽  
Jinfeng Liu ◽  
Meiru Si ◽  
...  
Keyword(s):  
Production Process ◽  
Large Scale ◽  
Commercial Production ◽  
Impact Factors ◽  
Scale Production ◽  
Large Scale Production ◽  
Foaming Behavior ◽  
Production Strategies ◽  
Potential Applications ◽  
And Control

AbstractRhamnolipids have recently attracted considerable attentions because of their excellent biosurfactant performance and potential applications in agriculture, environment, biomedicine, etc., but severe foaming causes the high cost of production, restraining their commercial production and applications. To reduce or eliminate the foaming, numerous explorations have been focused on foaming factors and fermentation strategies, but a systematic summary and discussion are still lacking. Additionally, although these studies have not broken through the bottleneck of foaming, they are conducive to understanding the foaming mechanism and developing more effective rhamnolipids production strategies. Therefore, this review focuses on the effects of fermentation components and control conditions on foaming behavior and fermentation strategies responded to the severe foaming in rhamnolipids fermentation and systematically summarizes 6 impact factors and 9 fermentation strategies. Furthermore, the potentialities of 9 fermentation strategies for large-scale production are discussed and some further strategies are suggested. We hope this review can further facilitate the understanding of foaming factors and fermentation strategies as well as conducive to developing the more effective large-scale production strategies to accelerate the commercial production process of rhamnolipids.

Microwave Curing and Drying of Cellular Concrete

1992 ◽  
Vol 269 ◽  
Author(s):  
Walter M. van Loock
Keyword(s):  
Production Process ◽  
Feasibility Study ◽  
Building Material ◽  
Large Scale ◽  
Scale Production ◽  
Volumetric Heating ◽  
Microwave Curing ◽  
Thermal Insulator ◽  
Large Scale Production ◽  
Cellular Concrete

ABSTRACTTraditionally the heating and curing of cellular concrete takes place in an autoclave. This building material is a thermal insulator so the curing and drying processes are energy and time consuming. Volumetric heating is an attractive method for improving the throughputs.A feasibility study leads to the conclusion that microwave processing is not likely to be applied in a large scale production process.

scholarly journals Upstream process optimization and micro- and macrocarrier screening for large-scale production of the oncolytic H-1 protoparvovirus

2021 ◽  
Author(s):  
Daniel Wohlfarth ◽  
Veronika Frehtman ◽  
Marcus Müller ◽  
Martin Vogel ◽  
Linh Minh Phuc Phan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Production Process ◽  
Large Scale ◽  
Culture Medium ◽  
Feeding Strategy ◽  
Virus Yield ◽  
Seeding Density ◽  
Scale Production ◽  
Cell Culture Medium ◽  
Transfer Capability

Abstract The oncolytic virus H-1PV is a promising candidate for various cancer treatments. Therefore, production process needs to be optimized and scaled up for future market release. Currently, the virus is produced with minimum essential medium in 10-layer CellSTACK® chambers with limited scalability, requiring a minimum seeding density of 7.9E3 cells/cm2. Production also requires a 5% fetal bovine serum (FBS) supplementation and has a virus yield up to 3.1E7 plaque-forming units (PFU)/cm2. Using the animal-free cell culture medium VP-SFM™ and a new feeding strategy, we demonstrate a yield boost by a mean of 0.3 log while reducing seeding density to 5.0E3 cells/cm2 and cutting FBS supplementation by up to 40% during the production process. Additionally, FBS is completely removed at the time of harvest. Eleven commercial micro- and macrocarriers were screened regarding cell growth, bead-to-bead transfer capability, and virus yield. We present a proof-of-concept study for producing H-1PV on a large scale with the microcarrier Cytodex® 1 in suspension and a macrocarrier for a fixed-bed iCELLis® bioreactor. A carrier-based H-1PV production process combined with an optimized cell culture medium and feeding strategy can facilitate future upscaling to industrial-scale production. Key points • Virus yield increase and FBS-free harvest after switching to cell culture medium VP-SFM™. • We screened carriers for cell growth, bead-to-bead transfer capability, and H-1PV yield. • High virus yield is achieved with Cytodex® 1 and macrocarrier for iCellis® in Erlenmeyer flasks.

scholarly journals Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

Revista CERES ◽  
2013 ◽  
Vol 60 (2) ◽  
pp. 152-160 ◽  
Author(s):  
Leticia Mascarenhas Pereira Barbosa ◽  
Vespasiano Borges de Paiva Neto ◽  
Leonardo Lucas Carnevalli Dias ◽  
Reginaldo Alves Festucci-Buselli ◽  
Rodrigo Sobreira Alexandre ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Culture Medium ◽  
Shoot Proliferation ◽  
Cellular Level ◽  
Fragaria X Ananassa ◽  
Scale Production ◽  
Ms Medium ◽  
Large Scale Production

In vitro propagation has become an effective practice for large-scale production of strawberry plants. The objective of this study was to evaluate the hyperhydricity and the multiplication capacity of two strawberry varieties (Fragaria x ananassa Duch. 'Dover' and 'Burkley') propagated in vitro. Plants maintained in MS medium supplemented with 1.0 mg L-1 BA were individualized and transferred to the same medium solidified with Agar (6.5 g L-1) or Phytagel® (2.5 g L-1) and BA at different concentrations (0; 0.5; 1.0; 2.0 and 3.0 mg L-1). Biochemical and anatomical analyses were carried out, as well as the analysis of the morphological hyperhydricity characteristics. The analysis of data showed: a) the increase in cytokinin concentration increased hyperhydricity frequency in both varieties; b) at concentrations up to 2.0 mg L-1 BA, the replacement of Agar by Phytagel® induced a higher formation of hyperhydric shoots; and c) the addition of BA induced oxidative stress, which is characterized by increased antioxidant activity and lipid peroxidation, as well as alterations at the cellular level, such as malformation of stomata and epidermal cells. In conclusion, the culture medium containing 0.5 mg L-1 BA solidified with Agar provided lower hyperhydricity percentages in association with higher rates of shoot proliferation in strawberry.

Effects of various planting and harvesting times on the yield, HCN, dry-matter accumulation and starch content of four cassava varieties in a tropical rainforest region

1983 ◽  
Vol 101 (3) ◽  
pp. 633-636 ◽  
Author(s):  
G. O. Kayode
Keyword(s):  
Dry Matter ◽  
Large Scale ◽  
Tropical Rainforest ◽  
Starch Content ◽  
Human Consumption ◽  
Dry Matter Accumulation ◽  
Scale Production ◽  
Large Scale Production ◽  
Increasing Demand ◽  
Cassava Varieties

SUMMARYResults of a 2-year study in a rainforest zone of south-western Nigeria to determine the effects of various planting and harvesting times on the yield, HCN, dry-matter accumulation and starch contents of cassava showed that times of planting and harvesting significantly affected all the variables studied. May planting gave highest yield arid starch contents, which suggests that large-scale production of cassava for industrial starch and human consumption should be planted in the month of May.The harvesting time experiment shows that cassava tuber will continue to increase until the 24th month, although, because of increasing demand for land, it will not be economic to leave cassava on the field for more than 15 months. For optimum starch and dry-matter accumulation, cassava should be harvested between 12 and 15 months.

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