Corynebacterium cyclohexanicum n. sp.: a cyclohexanecarboxylic acid utilizing bacterium

1973 ◽  
Vol 19 (8) ◽  
pp. 937-942 ◽  
Author(s):  
Tai Tokuyama ◽  
Toshi Kaneda
Keyword(s):  
Benzoic Acid ◽  
Yeast Extract ◽  
Gram Positive ◽  
Separate Species ◽  
Biochemical Characteristics ◽  
Cyclohexanecarboxylic Acid ◽  
Bacterium Strain

A cyclohexanecarboxylic acid utilizing bacterium, strain MU, has been isolated from soil and its taxonomic and physiological nature studied. The organism requires biotin or an unidentified factor present in yeast extract for growth, and can use one of various acids, alcohols, and carbohydrates as the sole carbon source.As the organism is a non-motile rod, gram-positive, non-sporing, not acid-fast, catalase-positive, and aerobic, it is identified as a Corynebacterium. Various growth and biochemical characteristics show that the organism is closely related to Corynebacterium equi but differs in several characteristics. Hence, a separate species, Corynebacterium cyclohexanicum is proposed for strain MU because of its unique use of cyclohexanecarboxylic acid. Results from manometric experiments indicate that its metabolism of cyclohexanecarboxylic acid is closely related to the well-established metabolic scheme of benzoic acid.

Isolation and identification of N2-fixing Pseudomonas associated with wetland rice

1983 ◽  
Vol 29 (8) ◽  
pp. 867-873 ◽  
Author(s):  
W. L. Barraquio ◽  
J. K. Ladha ◽  
I. Watanabe
Keyword(s):  
New Species ◽  
Yeast Extract ◽  
Heterotrophic Bacteria ◽  
Fluorescent Antibody ◽  
Wetland Rice ◽  
Isolation And Identification ◽  
Biochemical Characteristics ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
A New Species

Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.

The metabolism of cyclohexanecarboxylic acid and 3-cyclohexenecarboxylic acid by Pseudomonas putida

1982 ◽  
Vol 28 (12) ◽  
pp. 1324-1329 ◽  
Author(s):  
E. R. Blakley ◽  
B. Papish
Keyword(s):  
Benzoic Acid ◽  
Pseudomonas Putida ◽  
Sole Source ◽  
Oxidation Pathway ◽  
Cyclohexanecarboxylic Acid

A strain of Pseudomonas putida grew rapidly on cyclohexanecarboxylic acid as a sole source of carbon. A CoA-mediated β-oxidation pathway was induced for the metabolism of the compound. The organism could not utilize 3-cyclohexenecarboxylic acid as a sole source of carbon for growth, but cells grown on gluconate in the presence of 3-cyclohexenecarboxylic acid were induced to metabolize cyclohexanecarboxylic acid, benzoic acid, and catechol. Evidence is presented that 3-cyclohexenecarboxylic acid was slowly metabolized by a β-oxidation pathway and by a pathway involving benzoic acid as an intermediate. For this strain of Pseudomonas putida, 3-cyclohexenecarboxylic acid acts as an oxidizable, nongrowth substrate and induces the metabolism of cyclohexanecarboxylic acid and benzoic acid.

scholarly journals A selective medium for the isolation of the acinetobacter genus bacteria

1980 ◽  
Vol 75 (3-4) ◽  
pp. 11-21 ◽  
Author(s):  
Altair A. Zebral
Keyword(s):  
Yeast Extract ◽  
Crystal Violet ◽  
Sodium Citrate ◽  
Agar Medium ◽  
Selective Medium ◽  
Phenol Red ◽  
Gram Positive ◽  
Gram Negative ◽  
Fermentative Activity

A selective and differencial medium was developed for the isolation of Acinetobacter genus bacteria. This Acinobacter Agar Medium (p.H + 7.4) contains in grams per litre: thiotone, 10; yeast extract, 3; naC1, 5; saccharose, 10; mannitol, 10; sodium citrate, 0.5; sodium desoxycholate, 0.1; crystal violet, 0.00025; phenol red, 0.04 and agar-agar 15. This medium has the advantage of inhibiting the growth of cocci and Gram-positive bacilli, by the use of sodium citrate and sodium desoxycholate associated with the crystal violet; and of differentiating the Gram-negative bacilli from the Enterobacteriaceae, through the fermentative activity upon the saccharose and/or mannitol, contrasting with the complete inactivity of the Acinetobacter genus bacteria over those substances.

Significant effect of base on the improvement of selectivity in the hydrogenation of benzoic acid over NiZrB amorphous alloy supported on γ-Al2O3

2015 ◽  
Vol 5 (6) ◽  
pp. 3281-3287 ◽  
Author(s):  
Xin Wen ◽  
Yingying Cao ◽  
Xianliang Qiao ◽  
Libo Niu ◽  
Li Huo ◽  
...  
Keyword(s):  
Amorphous Alloy ◽  
Benzoic Acid ◽  
Cyclohexanecarboxylic Acid

The selectivity for cyclohexanecarboxylic acid was significantly enhanced by adding small amounts of bases in benzoic acid hydrogenation.

scholarly journals Biodegradation ofn-Alkylcycloalkanes and n-Alkylbenzenes via New Pathways in Alcanivorax sp. Strain MBIC 4326

2001 ◽  
Vol 67 (4) ◽  
pp. 1970-1974 ◽  
Author(s):  
Tapan K. Dutta ◽  
Shigeaki Harayama
Keyword(s):  
Carboxylic Acid ◽  
Benzoic Acid ◽  
Side Chain ◽  
Alkyl Side Chain ◽  
Cyclohexanecarboxylic Acid ◽  
Long Chain

ABSTRACT The degradation of long-chain n-alkylbenzenes andn-alkylcyclohexanes by Alcanivorax sp. strain MBIC 4326 was investigated. The alkyl side chain of these compounds was mainly processed by β-oxidation. In the degradation ofn-alkylcyclohexanes, cyclohexanecarboxylic acid was formed as an intermediate. This compound was further transformed to benzoic acid via 1-cyclohexene-1-carboxylic acid.

Depression of ruminal digestion in adult sheep by aureomycin.

1952 ◽  
Vol 3 (4) ◽  
pp. 453 ◽  
Author(s):  
AW Turner ◽  
VE Hodgetts
Keyword(s):  
Body Weight ◽  
Organic Acids ◽  
Yeast Extract ◽  
Ruminal Fermentation ◽  
Gram Positive ◽  
Ruminal Fluid ◽  
Adult Sheep ◽  
Low Level ◽  
Ruminal Ph ◽  
Ruminal Digestion

After the consumption by adult sheep of an amount of aureomycin hydrochloride corresponding to 23.6-27.1 mg. per kg. body weight, the ruminal flora had fallen about 75 per cent. within two hours and remained a t this low level for at least two days, the survivors being predominantly Gram-positive rods and cocci. Ruminal fermentation was greatly depressed; the yield of organic acids reached only about 30 per cent. of the normal and was insufficient to lower the ruminal pH significantly. Appetite was lessened and body weight fell, but responded favourably to administration of ruminal fluid and yeast extract.

scholarly journals Isolation and Identification of Lactic Acid Bacteria on Boiler Chicken

Elkawnie ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 287
Author(s):  
Roisu Eny Mudawaroch ◽  
Setiyono Setiyono ◽  
Lies Mira Yusiati ◽  
Edi Suryanto
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Broiler Chicken ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Biochemical Characteristics ◽  
Genus Lactobacillus ◽  
Main Material

Abstract: The aims of this research were to isolate lactic acid bacteria from broiler chicken. The main material was a chicken boiler aged 30 days. The identification of LAB was carried out based on isolation and identification of morphological, phenotypic, and biochemical characteristics. Thirteen LAB isolates were obtained and 5 selected LAB isolates were identified based on growth levels, pH, and lactic acid levels. Five selected LAB isolates were identified as Lactobacillus genus which has Gram-positive, negative catalase, non-motility, hetero-fermentative, rod shapes,  rod-colony shapes, and cream colony color.Abstrak: Penelitian ini bertujuan untuk mengisolasi bakteri asam laktat dari ayam broiler. Bahan utamanya adalah ayam boiler berumur 30 hari. Identifikasi BAL dilakukan berdasarkan isolasi dan identifikasi karakteristik morfologi, fenotipik, dan biokimia. Tiga belas isolat BAL diperoleh dan 5 isolat BAL terpilih diidentifikasi berdasarkan tingkat pertumbuhan, pH, dan kadar asam laktat. Lima isolat BAL terpilih diidentifikasi sebagai genus Lactobacillus yang memiliki Gram-positif, katalase negatif, non-motilitas, hetero-fermentatif, bentuk batang, bentuk koloni batang, dan warna koloni krem.

scholarly journals SYNTHESIS OF SOME NOVEL TRIAZOLE DERIVATIVES AS SCHIFF BASES AND THEIR ANTIMICROBIAL EVALUATION

2016 ◽  
Vol 8 (9) ◽  
pp. 28
Author(s):  
Vaibhav Sharma ◽  
Dinesh Kumar Mehta ◽  
Rina Das ◽  
Preet Kawal Kaur
Keyword(s):  
Schiff Base ◽  
Benzoic Acid ◽  
Analytical Techniques ◽  
Aromatic Aldehydes ◽  
Gram Positive ◽  
Gram Negative ◽  
H Nmr ◽  
Schiff Base Derivatives ◽  
Weak Activity ◽  
Anti Fungal

<p><strong>Objective: </strong>This work involves the synthesis of some novel schiff base derivatives synthesized from <em>p-</em>amino benzoic acid.</p><p><strong>Methods: </strong>A series of 4-[4-(arylidene amino-5-mercapto-4H-[1, 2, 4] triazol-3-yl]-benzoic acid complexes were synthesized from 4-(4-amino-5-mercapto-4H-[1, 2, 4] triazol-3-yl)-benzoic acid by reaction with different aromatic aldehydes. All the synthesized schiff base derivatives were characterized by using analytical techniques (FT-IR, <sup>1</sup>H NMR and Mass spectroscopy). The title compounds were evaluated for antibacterial activity against Gram-positive bacteria (<em>Staphylococcus aureus </em>and <em>Streptococcus pneumoniae) </em>and Gram-negative bacteria (<em>Escherichia coli and Pseudomonas aeruginosa)</em> and anti-fungal activity against (<em>Candida albicans </em>and <em>Aspergillus niger).</em></p><p><strong>Results: </strong>Schiff base derivatives 5(a-h) were synthesized in good yields and showed antimicrobial activity, among them, compounds 5c, 5d, 5e and 5f were significantly active against gram positive, gram negative bacterial and fungal strains and rest of compounds showed moderate to weak activity.</p><p><strong>Conclusion: </strong>The Schiff base obtained showed variation in the antimicrobial and antifungal activity, based on the structure of the substituted aromatic aldehydes.</p>

Antagonism by a Gram-positive coccus

1970 ◽  
Vol 16 (8) ◽  
pp. 661-665 ◽  
Author(s):  
T. J. Trust
Keyword(s):  
Cell Wall ◽  
Biological Properties ◽  
Cell Wall Synthesis ◽  
Gram Positive ◽  
Biochemical Characteristics ◽  
Gram Positive Bacteria ◽  
Solid Media ◽  
Positive Coccus

A Sarcina sp. has been isolated which is antagonistic to the growth of a number of Gram-positive bacteria when grown on solid media. The morphological and biochemical characteristics of the organism are described. The biological properties of the inhibitor have been examined. The data suggest that the inhibitor may interfere with cell wall synthesis.

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