Quinate metabolism in Pseudomonas aeruginosa

1972 ◽  
Vol 18 (12) ◽  
pp. 1817-1824 ◽  
Author(s):  
W. M. Ingledew ◽  
C. C. Tai
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Dehydrogenase Activity ◽  
Acinetobacter Calcoaceticus ◽  
Degradation Pathway ◽  
Constant Volume ◽  
Enzyme Analysis ◽  
Single Defect ◽  
Relationship Of

Data obtained with the Warburg constant-volume respirometer and from enzyme analysis have demonstrated that the method of regulation of the "hydroaromatic" degradation pathway found in Pseudomonas aeruginosa is vastly different from that previously discovered in Acinetobacter calcoaceticus and perhaps also significantly different from P. putida. Two mutants have been analyzed: the first with multiple impairment of degradative abilities; the second with a single defect, i.e. a lack of quinic dehydrogenase activity. Quinic dehydrogenase has also been shown to function for shikimate utilization in P. aeruginosa, even though 5-dehydroshikimate reductase in vitro catalyzes the same reaction. The possible relationship of these findings to pyocyanine formation is discussed.

scholarly journals From in vitro to in cellulo: structure–activity relationship of (2-nitrophenyl)methanol derivatives as inhibitors of PqsD in Pseudomonas aeruginosa

2014 ◽  
Vol 12 (32) ◽  
pp. 6094-6104 ◽  
Author(s):  
Michael P. Storz ◽  
Giuseppe Allegretta ◽  
Benjamin Kirsch ◽  
Martin Empting ◽  
Rolf W. Hartmann
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Relationship Of ◽  
Derivatives Of

More than 60 derivatives of (2-nitrophenyl)methanol were synthesized and evaluated regarding their potency to inhibit PqsD. In vitro and in cellulo structure–activity relationships were derived.

scholarly journals In vitro and in vivo activities of LB10522, a new catecholic cephalosporin.

1996 ◽  
Vol 40 (8) ◽  
pp. 1825-1831 ◽  
Author(s):  
M Y Kim ◽  
J I Oh ◽  
K S Paek ◽  
Y Z Kim ◽  
I C Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Klebsiella Oxytoca ◽  
Acinetobacter Calcoaceticus ◽  
Beta Lactamase ◽  
Salmonella Spp

In vitro activity of LB10522 was compared with those of cefpirome, ceftazidime, ceftriaxone, and cefoperazone against clinical isolates. Against gram-positive bacteria, LB10522 was most active among the compounds tested. It was fourfold more active than cefpirome against methicillin-susceptible Staphylococcus aureus and Enterococcus faecalis. LB10522 was highly effective against most members of the family Enterobacteriaceae tested. Ninety percent of isolates of Escherichia coli, Klebsiella oxytoca, Proteus vulgaris, Proteus mirabilis, and Salmonella spp. were inhibited at a concentration of < or = 0.5 micrograms/ml. These activities were comparable to those of cefpirome. Against Pseudomonas aeruginosa, LB10522 with a MIC at which 90% of the isolates are inhibited of 2 micrograms/ml was 16- and 32-fold more active than ceftazidime and ceftazidime against systemic infections caused by Staphylococcus aureus giorgio, Streptococcus pneumoniae III, Pseudomonas aeruginosa 1912E, Escherichia coli 851E, Proteus mirabilis 1315E, Serratia marcescens 1826E, and Acinetobacter calcoaceticus Ac-54. LB10522 was very resistant to hydrolysis by various beta-lactamases such as TEM-3, TEM-7, SHV-1, FEC-1, and P-99. LB10522 did not induce beta-lactamase in Enterobacter cloacae 1194E, although most of the reference cephalosporins acted as inducers of beta-lactamase in this strain. Time-kill study showed that LB10522, at concentrations of two or four times the MIC, had a rapid bactericidal activity against Staphylococcus aureus 6538p, Escherichia coli 851E, and Pseudomonas aeruginosa 1912E.

scholarly journals Evaluation of the Bactericidal Activity of Fosfomycin in Combination with Selected Antimicrobial Comparison Agents Tested against Gram-Negative Bacterial Strains by Using Time-Kill Curves

2019 ◽  
Vol 63 (5) ◽  
Author(s):  
Robert K. Flamm ◽  
Paul R. Rhomberg ◽  
Jill M. Lindley ◽  
Kim Sweeney ◽  
E. J. Ellis-Grosse ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Species Complex ◽  
Antimicrobial Agents ◽  
Acinetobacter Calcoaceticus ◽  
Synergistic Activity ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Content Type ◽  
Time Kill

ABSTRACT The effects of combining fosfomycin with various antimicrobial agents were evaluated in vitro by broth microdilution checkerboard and time-kill kinetic studies. Checkerboard analyses were used to evaluate the following 30 Gram-negative isolates: 5 Pseudomonas aeruginosa, 5 Acinetobacter baumannii-Acinetobacter calcoaceticus species complex, and 20 Enterobacteriaceae isolates. No isolate exhibited antagonism when fosfomycin was tested in combination, and synergy was observed in more than 25% of the drug combinations tested. The most frequent instances of synergy occurred when testing fosfomycin with β-lactams. Two isolates of Pseudomonas aeruginosa, 2 of Klebsiella pneumoniae, and 1 of the A. baumannii-A. calcoaceticus species complex that exhibited synergy when fosfomycin was tested in combination were subjected to time-kill kinetic analyses for confirmation. Time-kill assays confirmed synergistic activity. These data indicated that combination therapy with fosfomycin may be beneficial.

scholarly journals Sensibilidade bacteriana a antimicrobianos usados na prática médica - Ribeirão Preto (SP) - 1994

1996 ◽  
Vol 29 (2/3) ◽  
pp. 278-284
Author(s):  
Roberto Martinez ◽  
Rosa Helena A. Romanello Gironi ◽  
Vanda R. Dos Santos
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Acinetobacter Calcoaceticus ◽  
De Medicina

Apresenta-se a situação da susceptibilidade bacteriana a antimicrobianos, em amostras isoladas em 1994, no Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto-USP. A resistência do Staphylococcus aureus à oxacilina foi verificada em 17% das amostras de infecções da comunidade, e em 51% dos casos hospitalares. Os valores correspondentes para o Staphylococcus epidermidis foram, respectivamente, 19% e 39%. A resistência in vitro à penicilina foi observada em 7% das amostras de pneumococo, e em 20% das amostras de enterococo. Com exceção de poucas amostras, todos os cocos Gram-positivos eram sensíveis à vancomicina e teicoplanina. Com relação aos bacilos Gram-negativos, a sensibilidade in vitro das enterobactérias foi baixa para a ampicilina, carbenicilina e cotrimoxazole (26% a 53%), intermediária para cefalotina, cloranfenicol e cefoxitina (64% a 82%) e alta para as cefalosporinas de terceira geração, amicacina, fluoroquinolonas, aztreonam e imipenem (93% a 99%). Acima de 90% das amostras de Pseudomonas aeruginosa foram sensíveis à cefatazidina, aztreonam e imipenem. O Acinetobacter calcoaceticus, causa freqüente de infecção hospitalar, mostrou alta sensibilidade (98%) ao imipenem, porém resistência acentuada a outros antiinfeciosos. Conclui-se comentando sobre a escolha de antimicrobianos para o tratamento de infecções comunitárias e hospitalares.

STEROID HORMONE METABOLISM IN RESPONSIVE TISSUES IN VITRO

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S279-S294 ◽  
Author(s):  
Paul Robel
Keyword(s):  
Steroid Hormone ◽  
Physiological Activity ◽  
Physiological State ◽  
Target Cells ◽  
Target Tissue ◽  
Hormone Metabolism ◽  
Metabolizing Enzymes ◽  
Relationship Of

ABSTRACT Of the information available on steroid hormone metabolism in responsive tissues, only that relating hormone metabolism to physiological activity is reviewed, i. e. metabolite activity in isolated in vitro systems, binding of metabolites to target tissue receptors, specific steroid hormone metabolizing enzymes and relationship of hormone metabolism to target organ physiological state. Further, evidence is presented in the androgen field, demonstrating 5α-reduced metabolites, formed inside the target cells, as active compounds. This has led to a consideration of testosterone as a »prehormone«. The possibility that similar events take place in tissues responding to progesterone is discussed. Finally, the role of hormone metabolism in the regulation of hormone availability and/or renewal in target cells is discussed. In this context, reference is made to the potential role of plasma binding proteins and cytosol receptors.

Bioactivity of Phenolic Compounds Extracted from Strawberry against Formation of Pseudomonas aeruginosa Biofilm

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Baydaa Hussein ◽  
Zainab A. Aldhaher ◽  
Shahrazad Najem Abdu-Allah ◽  
Adel Hamdan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phenolic Compounds ◽  
Biofilm Formation ◽  
Opportunistic Infections ◽  
Formation Rate ◽  
Hydrocarbon Chain ◽  
Health Concern ◽  
Gas Chromatography Mass Spectrometry ◽  
Phenolic Contents

Background: Biofilm is a bacterial way of life prevalent in the world of microbes; in addition to that it is a source of alarm in the field of health concern. Pseudomonas aeruginosa is a pathogenic bacterium responsible for all opportunistic infections such as chronic and severe. Aim of this study: This paper aims to provide an overview of the promotion of isolates to produce a biofilm in vitro under special circumstances, to expose certain antibiotics to produce phenotypic evaluation of biofilm bacteria. Methods and Materials: Three diverse ways were used to inhibited biofilm formation of P.aeruginosa by effect of phenolic compounds extracts from strawberries. Isolates produced biofilm on agar MacConkey under certain circumstances. Results: The results showed that all isolates were resistant to antibiotics except sensitive to azithromycin (AZM, 15μg), and in this study was conducted on three ways to detect the biofilm produced, has been detected by the biofilm like Tissue culture plate (TCP), Tube method (TM), Congo Red Agar (CRA). These methods gave a clear result of these isolates under study. Active compounds were analyzed in both extracts by Gas Chromatography-mass Spectrometry which indicate High molecular weight compound with a long hydrocarbon chain. Conclusion: Phenolic compounds could behave as bioactive material and can be useful to be used in pharmaceutical synthesis. Phenolic contents which found in leaves and fruits extracts of strawberries shows antibacterial activity against all strains tested by the ability to reduce the production of biofilm formation rate.

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