Lysosomes and the "toxicity" of Rickettsials. III. Response of L cells infected with egg-attenuated C. psittaci 6BC strain

1972 ◽  
Vol 18 (8) ◽  
pp. 1343-1348 ◽  
Author(s):  
Nonna Kordová ◽  
Linda Poffenroth ◽  
John C. Wilt
Keyword(s):  
Cell Culture ◽  
Acid Phosphatase ◽  
Comparative Studies ◽  
Post Infection ◽  
Marked Contrast ◽  
Lysosomal Acid ◽  
L Cells ◽  
L Cell ◽  
Infected Cells

During the infection of L cells (clone 60 and 929) with egg-grown C. psittaci 6BC strain, lyosomes retained their integrity and L cells were not injured. Infected cells proliferated more intensely and showed higher mitotic indices from 24 h post infection than noninfected control cells. Chlamydial inclusions were released from the cytoplasm of infected L cells by pseudopodia-like extrusions. These events were in marked contrast to the effect of the L cell grown C. psittaci 6BC strain which caused activation of lysosomal acid phosphatase and damage of L cells. Previous comparative studies of the host lysosomal response to egg-grown as compared to cell culture grown C. psittaci 6BC strain in cultured macrophages and L cells are discussed.

Lysosomes and the "toxicity" of Rickettsials. II. Non-cytocidal interactions of egg-grown C. psittaci 6BC and in vitro macrophages

1972 ◽  
Vol 18 (6) ◽  
pp. 869-873 ◽  
Author(s):  
Nonna Kordová ◽  
Linda Poffenroth ◽  
John C. Wilt
Keyword(s):  
Acid Phosphatase ◽  
Cell Damage ◽  
Giant Cells ◽  
Host Cells ◽  
Marked Contrast ◽  
Lysosomal Acid ◽  
Cytoplasmic Vacuoles ◽  
L Cell

During the infection of cultured mouse peritoneal phagocytes with egg-grown C. psittaci 6BC strain, lysosomes retained their integrity and host cells were not damaged. Infected monocytes showed greater ability than uninfected monocytes to spread and transform into giant cells containing enlarged nuclei and masses of cytoplasm with clear cytoplasmic vacuoles. Chlamydial particles were released from the cytoplasm of infected phagocytes by pseudopodia-like extrusions. These events were in marked contrast to the effect of L cell grown C. psittaci 6BC strain which caused early leakage of lysosomal acid phosphatase into the cytoplasm of macrophages and induced a rapidly progressive irreversible cell damage (14).

The Demonstration of Human Prostatic Acid Phosphatase (Pap) With Phosphorylcholine

1976 ◽  
Vol 34 ◽  
pp. 88-89
Author(s):  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Prostate Gland ◽  
Prostatic Acid Phosphatase ◽  
Human Prostate ◽  
Rat Tissues ◽  
Specific Substrate ◽  
Acid Phosphatases ◽  
Lysosomal Acid ◽  
Cacodylate Buffer

As previously reported (1, 2) phosphorylcholine (PC) is a specific substrate for prostatatic acid phosphatase (PAP) as opposed to other acid phosphatases, e.g., lysosomal acid phosphatase. The specificity of PC for PAP is due to the pentavalent nitrogen in PC, a feature that renders PC resistant to hydrolysis by all other acid phosphatases. Detailed comparative cytochemical results in rat tissues are in press. This report deals with ultracytochemical results applying the method to normal and pathological human prostate gland.Fresh human prostate was obtained from 7 patients having transurethral resections or radical prostatectomies. The tissue was fixed in 3% glutaraldehyde- 0.1 M cacodylate buffer (pH 7.4) for 15 min, sectioned at 50 μm on a Sorvall TC-2 tissue sectioner, refixed for a total of 2 hr, and rinsed overnight in 0.1 M cacodylate buffer (pH 7.4)-7.5% sucrose.

Prostatic Acid Phosphatase (PAP) Differentiated Ultracytochemically from Lysosomal Acid Phosphate in the Rat with a PAP/Specific Substrate, Phosphorylcholine

1975 ◽  
Vol 33 ◽  
pp. 450-451
Author(s):  
W. Allen Shannon ◽  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Phosphate Buffer ◽  
Prostatic Carcinoma ◽  
Prostatic Acid Phosphatase ◽  
Chemotherapeutic Agents ◽  
Specific Substrate ◽  
Acid Phosphate ◽  
Lysosomal Acid ◽  
Design And Synthesis ◽  
New Chemotherapeutic Agents

During the design and synthesis of new chemotherapeutic agents for prostatic carcinoma based on phosphorylated agents which might be enzyme-activated to cytotoxicity, phosphorylcholine, [(CH3)3+NCH2CH2OPO3Ca]Cl-, has been indicated to be a very specific substrate for prostatic acid phosphatase (PAP). This phenomenon has led to the development of specific histochemical and ultracytochemical methods for PAP using modifications of the Gomori lead method for acid phosphatase. Comparative histochemical results in prostate and kidney of the rat have been published earlier with phosphorylcholine (PC) and β-glycerophosphate (βGP). We now report the ultracytochemical results.Minced tissues were fixed in 3% glutaraldehyde-0.1 M phosphate buffered (pH 7.4) for 1.5 hr and rinsed overnight in several changes of 0.05 M phosphate buffer (pH 7.0) containing 7.5% sucrose. Tissues were incubated 30 min to 2 hr in Gomori acid phosphatase medium (2) containing 0.1 M substrate, either PC or βGP.

Tartrate-resistant acid phosphatase is not an exclusive marker for mouse osteoclasts in cell culture

Bone ◽  
1991 ◽  
Vol 12 (2) ◽  
pp. 81-87 ◽  
Author(s):  
W.E. Modderman ◽  
A.C. Tuinenburg-Bol Raap ◽  
P.J. Nijweide
Keyword(s):  
Cell Culture ◽  
Acid Phosphatase ◽  
Tartrate Resistant Acid Phosphatase

Generation and Maintenance of Immune Interferon-Producing Cells Induced by Allogeneic Stimulation in Mice

1980 ◽  
Vol 29 (2) ◽  
pp. 383-389
Author(s):  
Yasuhiko Ito ◽  
Hiizu Aoki ◽  
Yoshinobu Kimura ◽  
Michiko Takano ◽  
Koichiro Maeno ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Culture Fluid ◽  
Suppressive Effect ◽  
Interferon Production ◽  
Spleen Cells ◽  
Immune Interferon ◽  
L Cells ◽  
L Cell ◽  
Interferon Activity ◽  
Allogeneic Stimulation

When spleen cells derived from C57BL/6 mice immunized with L cells 7 days previously were cocultured with antigenic cells, immune interferon appeared in the culture fluid. We analyzed the tissue distribution of the immune interferon-producing cells (IIPC) which appeared in various lymphoid organs after allogeneic stimulation. Although fluid from cocultures of L-cell-sensitized thymocytes and L-cells could not detect interferon activity consistently, small numbers of IIPC could be detected by using the enumeration method of IIPC. The generation, maintenance, and nature of IIPC emerging in the spleen were different depending on how the host mice were immunized. Multiple antigenic stimulations were more effective and induced longer-lasting immune interferon production than a single stimulation. IIPC induced by a single stimulation appeared to be sensitive to cortisone, vinblastine, and cyclophosphamide and were relatively short lived. In contrast, IIPC induced by multiple stimulations seemed to be partially resistant to these drugs and long lived. When mice were immunized with intact L-cells, carrageenan, a known antimacrophage agent, had no effect on immune interferon production. However, when mice were immunized with solubilized L-cell antigen, this drug displayed a suppressive effect on immune interferon production.

scholarly journals Efficient Plant Regeneration by Smashing Callus in Rice(Oriza sativa L.) Cell Culture.

1996 ◽  
Vol 13 (3) ◽  
pp. 291-295
Author(s):  
Akihiro OKAMOTO ◽  
Shinobu KISHINE ◽  
Takayasu HIROSAWA
Keyword(s):  
Cell Culture ◽  
Plant Regeneration ◽  
L Cell ◽  
Oriza Sativa

High Degree of Homology Between Primary Structure of Human Lysosomal Acid Phosphatase and Human Prostatic Acid Phosphatase

1989 ◽  
Vol 370 (1) ◽  
pp. 177-182 ◽  
Author(s):  
Christoph PETERS ◽  
Carola GEIER ◽  
Regina POHLMANN ◽  
Abdul WAHEED ◽  
Kurt VON FIGURA ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Primary Structure ◽  
Prostatic Acid Phosphatase ◽  
Lysosomal Acid ◽  
High Degree
Sign in / Sign up

Export Citation Format

Share Document