Oxidation of manganous salts of manganese by soil fungi

1972 ◽  
Vol 18 (6) ◽  
pp. 793-799 ◽  
Author(s):  
M. I. Timonin ◽  
W. I. Illman ◽  
T. Hartgerink

The microbiological analysis of soils from Ontario, Yukon, and Northwest Territories showed the presence of manganese-oxidizing bacteria, Actinomycetes, and fungi. Bacteria and Actinomycetes were predominant in boreal and tundra soils whereas fungi were predominant in the Ontario samples. The 18 species of fungi studied were able to oxidize 10 different compounds of manganese including a chelated form as well as an organic Mn2+ complex. Coniothyrium fuckelii, Periconia sp., P. circinata, P. macrospinosa, Phoma sp., and Sporothrix sp. oxidized all 10 Mn2+ compounds tested. One bacterial culture isolated from Arctic soil was able to oxidize only Mn2+ acetate and tartrate salts.The addition to Mn2+ sulfate medium of either calcium salts of several organic acids or various carbohydrates affected the growth of fungi as well as the nature of the end product of Mn2+ oxidation. Thus, the manganic granules produced by Cephalosporium sp. in the medium in the presence of glucose or starch were resistant to solubilization, dissolving only after 6 h in 1 N ammonium acetate (pH 7.0) containing 0.2% of hydroquinone. Granules produced by P. circinata in the presence of cellulose were insoluble in this solvent. On dissolution of the granules with 30% hydrochloric acid, the residue did not show the presence of bacteria under microscopic examination.

2012 ◽  
Vol 5 (4) ◽  
pp. 419-429 ◽  
Author(s):  
Ina Timling ◽  
D. Lee Taylor
Keyword(s):  

2019 ◽  
Vol 1 (2) ◽  
pp. 37-40
Author(s):  
Shinta Asarina ◽  
Nuni Haeruni

Glycerol is one of the ingredients for preserving bacterial culture. Glycerol has hygroscopic properties so that it can absorb water molecules from the environment and prevent wet mount preparations. The use of glycerol in making semipermanent worm egg preparations for microscopic examination was evaluated in this study. This research was conducted at the Microbiology and Parasitology Laboratory of the Faculty of Medicine, Padjadjaran University, Bandung. From the results of the study it was found that worm egg preparations with the addition of glycerol can last longer than those without glycerol. This method can be recommended for making semipermanent worm egg preparations for the purpose of parasitological examination in the laboratory and student laboratory


2019 ◽  
Vol 38 (04) ◽  
Author(s):  
Tahmina Begum ◽  
Zakirul K Islam ◽  
Mohammad Shohel Rana Siddiki ◽  
Raihan Habib ◽  
Harun Ur Rashid

The study was aimed to develop a probiotic beverage using Chhana whey and watermelon juice. The blends of whey and watermelon juice were optimized based on physicochemical and sensory evaluation. The bacterial culture was used as per 1% Lactobacillus acidophilus NIAI L-54 as a probiotic culture. The 75:25 blend of whey and watermelon fermented for 5 h at 37 oC obtained the highest sensory scores for overall acceptability. The shelf-life of the selected blend was examined using titratable acidity, pH, microbiological analysis as well as sensory evaluation. The results suggested that probiotic beverages can be preserved for 21 days in refrigerated conditions (4 oC) with higher acceptability along with probiotic bacterial count 4.00 Log10CFU /mL. It could be concluded that the whey-based watermelon beverage enabled by-product utilization with excellent nutritional and functional value.


Author(s):  
R. Kalirajan ◽  
R. Uma Rani ◽  
Vijay L. Parmar ◽  
R.R. Singh ◽  
N.B. Jadav

Six pluriparous Holstein Friesian cross bred cows of age between 4 years to 6 years with dermatophilosis were examined and treated with Homeopathic medicines.Direct microscopic examination of the impression smears of skin lesions and bacterial culture confirmed the organisms D. congolensis. The animals were treated with homeopathic medicines, Rhus tox 30 C, Sepia 30 C, Thuja 200X and Psorinum 30 C. Treated animals showed cessation of itching, oozing and shedding off scabs from 3rd day to 5th day and the crusts started falling off from the lesions between 3rd to 4th day of treatment. Hair regrowth started from 12 days and complete healing without any scarring was observed within 25 to 28 days . The disease did not recur on the treated animals during the follow up period of one year even during the next rainy season.


2012 ◽  
pp. 247-252
Author(s):  
Csaba Tamás Tóth ◽  
Zsuzsanna Szabó ◽  
Mária Csubák

In Hungary today is about 5 million hectares of agricultural land contaminated with ragweed. The ragweed problem a year is about 60 billion HUF to be paid, of which 30 billion are used to reduce the agricultural damage. Experiments with ragweed pollen has mainly been carried out in connection with terms of allergy. The other biochemical experiments and studies with this plant, have so far been the scientific horizons of public life, boosted the edge. We wanted to demonstrate that the ragweed, which is a weed, containsbiological active (for example: antifungal) compounds. For our experiments in the previous cycle of flowering, plants were collected manually, with its roots and with each plant part. The extraction of the substance from dry plant – meal was carried out using appropriate solvents. The biological activity of ragweed-extracts were tested against fungi isolated from soils and meadow with different mode of cultivation. Our results suggest that ragweed contains biologically active substances, which inhibit the growth of fungi, depending on the concentration of active ingredients of the plant.


1978 ◽  
Vol 24 (8) ◽  
pp. 909-914 ◽  
Author(s):  
Louise M. Nelson ◽  
D. Parkinson

Three bacterial isolates, a Pseudomonas sp., a Bacillus sp., and an Arthrobacter sp., commonly isolated from a hummocky sedge–moss meadow at Devon Island, N.W.T., Canada, were selected for further taxonomic characterization and for a study of the effects of temperature and limiting carbon source on growth. Pseudomonas M216 resembled P. putida and Bacillus M 153, B. carotarum. Arthrobacter M51 had growth-factor requirements which were more complex than those of any named species of that genus. The temperature ranges of growth indicated that Pseudomonas M216 and Arthrobacter M51 were psychrotrophic while Bacillus M153 was mesophilic. Growth in batch culture at limiting glucose concentrations enabled the calculation of Ks and Y values for each isolate. These were similar to those obtained for other organisms and Pseudomonas M216 and Bacillus M153 showed a high affinity for glucose. The nutritional versatility of Arthrobacter M51 and its ability to grow at low temperatures and the high growth rates and affinity of Pseudomonas M216 for low substrate concentrations may account for their competitive abilities in the natural environment, while the inability of Bacillus M153 to grow at low temperatures may limit its activity in tundra soils.


Author(s):  
Johan Werkelin ◽  
Maria Zevenhoven ◽  
Bengt-Johan Skrifvars ◽  
Mikko Hupa

The prediction of the ash behavior in combustion processes is enhanced by the determination of the ash-forming matter in the fuel. The scope of this work was to characterize Scandinavian wood fuels by studying the wood, bark and leaves from a spruce and an aspen. Chemical Fractionation, a step-wise leaching procedure that treat the fuel in water, ammonium acetate and hydrochloric acid, was used to determine the solubility of the ash elements. The resulting solutions were further analyzed on ionic species, which was coupled to the presence of salt compounds in the original biomass tissues. The leaching procedure dissolved nearly 100% of the ash-forming matter. The phosphorus (P), chlorine (Cl) and some of the sulfur (S) were readily leached with water and found present as HPO42−, SO42− and Cl− in the solutions. The simultaneous dissolving of potassium (K) in this step proved the presence of water soluble potassium salts in the biomasses. The remaining K after the water treatment and all magnesium (Mg) dissolved completely in the ammonium acetate step together with a large part of the tissues’ calcium (Ca) and manganese (Mn). The remaining Ca after the second step was leached in hydrochloric acid together with equivalent amounts of oxalate ions, C2O42−. This proved the presence of acid soluble calcium oxalate in the tissues, a salt with low solubility in neutral pH.


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