Overproduction of hisH and hisF gene products leads to inhibition of cell division in Salmonella

1972 ◽  
Vol 18 (5) ◽  
pp. 671-681 ◽  
Author(s):  
Michael L. Murray ◽  
Philip E. Hartman
Keyword(s):  
Cell Division ◽  
Carbon Source ◽  
Dna Synthesis ◽  
Salmonella Typhimurium ◽  
Nuclear Division ◽  
Direct Interaction ◽  
Pleiotropic Effects ◽  
Biosynthetic Enzymes ◽  
Gene Products ◽  
Low Carbon

Salmonella typhimurium derepressed for the histidine biosynthetic enzymes form wrinkled colonies at 37 °C on media containing a high (2%) content of metabolizable carbon source. "Wrinkledness" reflects accumulation of multinucleate filamentous cells caused by impaired cell division. At 42 °C, DNA synthesis and nuclear division are impaired. Addition of methionine at 42 °C permits DNA synthesis, nuclear division, and, under some conditions, cell division. However, multinucleate filaments are formed both on high and on low carbon source at 42 °C. The filaments contain nuclei that are peculiarly hypersensitive to inactivation with ultraviolet (uv.) light.Overproduction of both the hisH and hisF gene products is required for these pleiotropic effects of derepression. The hisH and hisF proteins, and even some of their enzymologically inactive forms, may cooperate to inhibit cell division by direct interaction with (a) sensitive cellular site(s).

Oxygen uptake during the cell cycle of the fission yeast Schizosaccharomyces pombe

1978 ◽  
Vol 33 (1) ◽  
pp. 399-411
Author(s):  
J. Creanor
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Oxygen Uptake ◽  
Dna Synthesis ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Nuclear Division ◽  
Synchronous Culture ◽  
Synchronous Cultures ◽  
The Many

Oxygen uptake was measured in synchronous cultures of the fission yeast Schizosaccharomyces pombe. The rate of oxygen uptake was found to increase in a step-wise manner at the beginning of the cycle and again in the middle of the cycle. The increases in rate were such that overall, oxygen uptake doubled in rate once per cell cycle. Addition of inhibitors of DNA synthesis or nuclear division to a synchronous culture did not affect the uptake of oxygen. In an induced synchronous culture, in which DNA synthesis, cell division, and nuclear division, but not ‘growth’ were synchronized, oxygen uptake increased continuously in rate and did not show the step-wise rises which were shown in the selection-synchronized culture. These results were compared with previous measurements of oxygen uptake in yeast and an explanation is suggested for the many different patterns which have been reported.

Inhibition of DNA synthesis and cell division in Salmonella typhimurium by azide

1974 ◽  
Vol 135 (4) ◽  
pp. 339-348 ◽  
Author(s):  
Z. Cieśla ◽  
Krystyna Mardarowicz ◽  
T. Klopotowski
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Salmonella Typhimurium ◽  
Inhibition Of Dna Synthesis

scholarly journals A dependent pathway of gene functions leading to chromosome segregation in Saccharomyces cerevisiae.

1982 ◽  
Vol 94 (3) ◽  
pp. 718-726 ◽  
Author(s):  
J S Wood ◽  
L H Hartwell
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Chromosome Segregation ◽  
Nuclear Division ◽  
Mitotic Cell ◽  
Mitotic Cell Cycle ◽  
Gene Products ◽  
Dependent Pathway

Methyl-benzimidazole-2-ylcarbamate (MBC) inhibits the mitotic cell cycle of Saccharomyces cerevisiae at a stage subsequent to DNA synthesis and before the completion of nuclear division (Quinlan, R. A., C. I. Pogson, and K, Gull, 1980, J Cell Sci., 46: 341-352). The step in the cell cycle that is sensitive to MBC inhibition was ordered to reciprocal shift experiments with respect to the step catalyzed by cdc gene products. Execution of the CDC7 step is required for the initiation of DNA synthesis and for completion of the MBC-sensitive step. Results obtained with mutants (cdc2, 6, 8, 9, and 21) defective in DNA replication and with an inhibitor of DNA replication (hydroxyurea) suggest that some DNA replication required for execution of the MBC-sensitive step but that the completion of replication is not. Of particular interest were mutants (cdc5, 13, 14, 15, 16, 17, and 23) that arrest cell division after DNA replication but before nuclear division since previous experiments had not been able to resolve the pathway of events in this part of the cell cycle. Execution of the CDC17 step was found to be a prerequisite for execution of the MBC-sensitive step; the CDC13, 16 and 23 steps are executed independently of the MBC-sensitive step; execution of the MBC-sensitive step is prerequisite for execution of the MBC-sensitive step; execution of the MBC-sensitive step is prerequisite for execution of the CDC14 and 23 steps. These results considerably extend the dependent pathway of events that constitute the cell cycle of S. cerevisiae.

scholarly journals Cell Division Inhibition in Salmonella typhimuriumHistidine-Constitutive Strains: an ftsI-Like Defect in the Presence of Wild-Type Penicillin-Binding Protein 3 Levels

1998 ◽  
Vol 180 (19) ◽  
pp. 5231-5234 ◽  
Author(s):  
David A. Cano ◽  
Chakib Mouslim ◽  
Juan A. Ayala ◽  
Francisco García-del Portillo ◽  
Josep Casadesús
Keyword(s):  
Cell Division ◽  
Carbon Source ◽  
Salmonella Typhimurium ◽  
Binding Protein ◽  
Penicillin Binding Protein ◽  
Wild Type ◽  
Septum Formation ◽  
Phenotypic Suppression ◽  
High Concentrations ◽  
Cell Division Inhibition

ABSTRACT Histidine-constitutive (Hisc) strains ofSalmonella typhimurium undergo cell division inhibition in the presence of high concentrations of a metabolizable carbon source. Filaments formed by Hisc strains show constrictions and contain evenly spaced nucleoids, suggesting a defect in septum formation. Inhibitors of penicillin-binding protein 3 (PBP3) induce a filamentation pattern identical to that of Hisc strains. However, the Hisc septation defect is caused neither by reduced PBP3 synthesis nor by reduced PBP3 activity. Gross modifications of peptidoglycan composition are also ruled out.d-Cycloserine, an inhibitor of the soluble pathway producing peptidoglycan precursors, causes phenotypic suppression of filamentation, suggesting that the septation defect of Hiscstrains may be caused by scarcity of PBP3 substrate.

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