Alteration of the rate of cell division independent of the rate of DNA synthesis in a mutant of Salmonella typhimurium

1972 ◽  
Vol 115 (2) ◽  
pp. 122-125 ◽  
Author(s):  
K. P. Shannon ◽  
R. J. Rowbury
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Salmonella Typhimurium

Inhibition of DNA synthesis and cell division in Salmonella typhimurium by azide

1974 ◽  
Vol 135 (4) ◽  
pp. 339-348 ◽  
Author(s):  
Z. Cieśla ◽  
Krystyna Mardarowicz ◽  
T. Klopotowski
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Salmonella Typhimurium ◽  
Inhibition Of Dna Synthesis

Overproduction of hisH and hisF gene products leads to inhibition of cell division in Salmonella

1972 ◽  
Vol 18 (5) ◽  
pp. 671-681 ◽  
Author(s):  
Michael L. Murray ◽  
Philip E. Hartman
Keyword(s):  
Cell Division ◽  
Carbon Source ◽  
Dna Synthesis ◽  
Salmonella Typhimurium ◽  
Nuclear Division ◽  
Direct Interaction ◽  
Pleiotropic Effects ◽  
Biosynthetic Enzymes ◽  
Gene Products ◽  
Low Carbon

Salmonella typhimurium derepressed for the histidine biosynthetic enzymes form wrinkled colonies at 37 °C on media containing a high (2%) content of metabolizable carbon source. "Wrinkledness" reflects accumulation of multinucleate filamentous cells caused by impaired cell division. At 42 °C, DNA synthesis and nuclear division are impaired. Addition of methionine at 42 °C permits DNA synthesis, nuclear division, and, under some conditions, cell division. However, multinucleate filaments are formed both on high and on low carbon source at 42 °C. The filaments contain nuclei that are peculiarly hypersensitive to inactivation with ultraviolet (uv.) light.Overproduction of both the hisH and hisF gene products is required for these pleiotropic effects of derepression. The hisH and hisF proteins, and even some of their enzymologically inactive forms, may cooperate to inhibit cell division by direct interaction with (a) sensitive cellular site(s).

Inhibition of DNA synthesis and cell division by a cell surface sialoglycopeptide

1989 ◽  
Vol 139 (2) ◽  
pp. 269-274 ◽  
Author(s):  
Heideh Fattaey ◽  
Terry C. Johnson ◽  
Hsin-Hwei Chou
Keyword(s):  
Cell Division ◽  
Cell Surface ◽  
Dna Synthesis ◽  
A Cell ◽  
Inhibition Of Dna Synthesis

scholarly journals Dem Anfang auf der Spur — die Suche nach DNA-Replikationsursprüngen

BIOspektrum ◽  
2021 ◽  
Vol 27 (3) ◽  
pp. 246-249
Author(s):  
Elisabeth Kruse ◽  
Stephan Hamperl
Keyword(s):  
Cell Division ◽  
Dna Synthesis ◽  
Genomic Dna ◽  
Genetic Material ◽  
Uniform Method ◽  
Replication Origins ◽  
Daughter Cells ◽  
Origins Of Replication ◽  
Mammalian Genomes ◽  
Comprehensive Manner

AbstractTimely and accurate duplication of DNA prior to cell division is a prerequisite for propagation of the genetic material to both daughter cells. DNA synthesis initiates at discrete sites, termed replication origins, and proceeds in a bidirectional manner until all genomic DNA is replicated. Despite the fundamental nature of these events, a uniform method that identifies origins of replication in a comprehensive manner is still missing. Here, we present currently available and discuss new approaches to map replication origins in mammalian genomes.

Changes of the DNA content of differentiating and adult macronuclei of the ciliate Loxodes magnus (Karyorelictida)

1980 ◽  
Vol 44 (1) ◽  
pp. 375-394
Author(s):  
N.N. Bobyleva ◽  
B.N. Kudrjavtsev ◽  
I.B. Raikov
Keyword(s):  
Cell Division ◽  
Dna Replication ◽  
Hydrochloric Acid ◽  
Dna Synthesis ◽  
Acid Hydrolysis ◽  
Gene Amplification ◽  
Dna Content

The DNA content of isolated micronuclei, differentiating macronuclei (macronuclear Anlagen), and adult macronuclei of Loxodes magnus was measured cytofluorimetrically in preparations stained with a Schiff-type reagent, auramine-SO2, following hydrochloric acid hydrolysis. The DNA content of the youngest macronuclear Anlagen proved to be the same as that of telophasic micronuclei (2 c). The Anlagen thus differentiate from micronuclei which are still in G1. The quantity of DNA in the macronuclear Anlagen thereafter rises to the 4-c level, simultaneously with DNA replication in the micronuclei which immediately follows mitosis. In non-dividing animals most micronuclei are already in G2. Adult macronuclei here contain on average 1.5 times more DNA than the micronuclei; their DNA content is about 5–6 c (in some individual nuclei, up to 10 c). These data are consistent with autoradiographic evidence indicating a weak DNA synthesis in the macronuclei of Loxodes and make likely the existence of partial DNA replication (e.g. gene amplification) in the macronuclei. The DNA content of adult macronuclei isolated from dividing animals proved to be significantly smaller than that of macronuclei isolated from non-dividing specimens of the same clone. In 3 clones studied, the former value amounted on average to 71–79, 78 and 95% of the latter, respectively. This drop of DNA content cannot be explained by ‘dilution’ of the old macronuclei with newly formed ones. The quantity of DNA in adult macronuclei thus seems to undergo cyclical changes correlated with cytokinesis, despite the fact that, in Loxodes magnus, the macronuclei themselves never divide and are simply segregated at every cell division. The macronuclei of Loxodes can be termed paradiploid or hyperdiploid.

Sign in / Sign up

Export Citation Format

Share Document