Intracellular development of a bacteriophage of Clostridium perfringens

1970 ◽  
Vol 16 (10) ◽  
pp. 983-988 ◽  
Author(s):  
D. E. Mahony ◽  
K. B. Easterbrook
Keyword(s):  
Clostridium Perfringens ◽  
Growth Cycle ◽  
Indicator Strain ◽  
Thin Sections ◽  
Head Diameter ◽  
Complete Lysis ◽  
One Step ◽  
Step Growth ◽  
Growth Experiments ◽  
Fluid Culture

A new bacteriophage of Clostridium perfringens was isolated which could cause complete lysis of its indicator strain in fluid culture. The adsorption characteristics and one-step growth experiments revealed that the phage rapidly adsorbed to the indicator strain and had a latent period of 45 min. Electron microscopy was performed on both thin sections and partially lysed preparations of infected bacteria at intervals throughout the growth cycle of the phage. This phage, named CPT4, has a head diameter of 64 nm and a striated tail 170 × 10 nm. In addition to these complete phages a number of "petit" phage forms was apparent in lysates. These forms were 55 nm in diameter, possessed no tail, and probably contained no nucleic acid.Morphogenic studies revealed that head forms appeared 30 min postinfection and that tails were present by 35 min. Assembly of mature phage particles seemed complete 40 min postinfection.

scholarly journals A temperate bacteriophage of Clostridium perfringens

1968 ◽  
Vol 14 (10) ◽  
pp. 1085-1093 ◽  
Author(s):  
D. E. Mahony ◽  
G. G. Kalz
Keyword(s):  
Electron Microscopy ◽  
Clostridium Perfringens ◽  
Deoxyribonucleic Acid ◽  
Solid Medium ◽  
Temperate Bacteriophage ◽  
Acridine Orange Staining ◽  
Lysogenic Strain ◽  
Step Growth ◽  
Growth Experiments ◽  
Diphenylamine Reaction

A temperate bacteriophage was isolated from a lysogenic strain of Clostridium perfringens. This lysogenic strain was inducible by ultraviolet light. Plaques produced on solid medium were small with turbid centers. Electron microscopy revealed that the phage has a polyhedral head, 80 × 70 mμ, and a striated tail, 200 mμ long.One-step growth experiments revealed latent and release periods of 45 minutes each. Phage yields were variable but, in general, quite low. The adsorption rate and temperature and pH stability of the phage were determined.The diphenylamine reaction and acridine orange staining suggested that the phage nucleic acid is deoxyribonucleic acid.

scholarly journals Replication of Simian Virus 40 Deoxyribonucleic Acid: Analysis of the One-Step Growth Cycle

1973 ◽  
Vol 11 (1) ◽  
pp. 98-106 ◽  
Author(s):  
Simone Manteuil ◽  
Jacqueline Pages ◽  
Dominique Stehelin ◽  
Marc Girard
Keyword(s):  
Deoxyribonucleic Acid ◽  
Simian Virus 40 ◽  
Growth Cycle ◽  
Simian Virus ◽  
One Step ◽  
Step Growth ◽  
The One

One-step growth experiments (cyanophages) v1

protocols.io ◽  
2015 ◽  
Author(s):  
Mathias Middelboe ◽  
Amy M ◽  
and Sif
Keyword(s):  
One Step ◽  
Step Growth ◽  
Growth Experiments

scholarly journals CHARACTERIZATION OF T4 MUTANTS THAT PARTIALLY SUPPRESS THE INABILITY OF T4rII TO GROW IN LAMBDA LYSOGENS

Genetics ◽  
1976 ◽  
Vol 83 (3) ◽  
pp. 477-487
Author(s):  
Theodore Homyk ◽  
Angel Rodriguez ◽  
Jon Weil
Keyword(s):  
Burst Size ◽  
Personal Communication ◽  
Growth Cycle ◽  
Plaque Morphology ◽  
One Step ◽  
Step Growth ◽  
The One ◽  
Phage Growth ◽  
L1 And L2

ABSTRACT In the course of isolating viable T4 deletions that affect plaque morphology (Homyk and Weil 1974), two closely linked point mutants, sip1 and sip2, were obtained. They map between genes t and 52, cause a reduction in plaque size and burst size, and partially suppress the lethality of rII mutants for growth in lambda lysogens. These characteristics demonstrate that sip1 and sip2 are similar to mutants previously reported by Freedman and Brenner (1972). In addition, D. Hall (personal communication) has shown that sip1 and sip2 are similar to the mutant farP85, which affects the regulation of a number of early genes (Chace and Hall 1975).—Sip suppression of rII mutants can be demonstrated in one-step growth experiments, even when both rII genes are completely deleted. This indicates that sip mutants do not simply reduce the level of rII gene products required for growth in a lambda lysogen. Instead, they alter the growth cycle so as to partially circumvent the need for any rII products.—Mutations at two other sites, designated L1 and L2, reverse the poor phage growth caused by sip and, in the one case tested, reverse the rII-suppressing ability of sip.

On-line monitoring of changes in host cell physiology during the one-step growth cycle of Bacillus phage Bam35

2007 ◽  
Vol 69 (1) ◽  
pp. 174-179 ◽  
Author(s):  
Rimantas Daugelavičius ◽  
Aušra Gaidelytė ◽  
Virginija Cvirkaitė-Krupovič ◽  
Dennis H. Bamford
Keyword(s):  
Host Cell ◽  
Growth Cycle ◽  
Cell Physiology ◽  
On Line ◽  
One Step ◽  
Bacillus Phage ◽  
Step Growth ◽  
The One

One-step growth experiments (bacteriophages) v1

protocols.io ◽  
2015 ◽  
Author(s):  
Mathias Middelboe ◽  
Amy M ◽  
and Sif
Keyword(s):  
One Step ◽  
Step Growth ◽  
Growth Experiments

Isolation and characterization of two bacteriophages of a stem-nodulating Rhizobium strain from Sesbania rostrata

1984 ◽  
Vol 30 (5) ◽  
pp. 521-525 ◽  
Author(s):  
Philippe de Lajudie ◽  
Didier Bogusz
Keyword(s):  
Burst Size ◽  
Growth Cycle ◽  
Sesbania Rostrata ◽  
Isolation And Characterization ◽  
Rhizobium Strains ◽  
One Step ◽  
Average Burst Size ◽  
Step Growth ◽  
Phage Growth

Two rhizobiophages, RS1 and RS2, were isolated in Senegal from a soil sample and dry stem nodules of Sesbania rostrata, a tropical legume that is infected by two categories of Rhizobium strains: "stem strains," which nodulate both roots and stems (type strain, ORS571), and "root strains," which induce effective nodules only on roots. Both phages were found to have a host range restricted to ORS571; all root strains were found to be resistant. By electron microscopy, phage RS1 showed an hexagonal head 63 nm wide and a tail 87 nm long; phage RS2 revealed an hexagonal head 60 nm wide. Characterization of phage growth cycle by one-step growth experiments showed that the latent period was ca. 75 min for RS1 and ca. 4 h for RS2, that the rise period lasted ca. 2 h for both RS1 and RS2, and that the average burst size was ca. 100 for RS1 and 130 for RS2. Temperature denaturation occurred at 60–65 °C (RS1) and 45–50 °C (RS2). Serum neutralization tests revealed that the phages were not serologically related. In contrast to RS1, RS2 appeared to be temperate, since stable lysogens were isolated.

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