A marine isolate of Pseudomonas nigrifaciens. I. Classification and nutrition

1968 ◽  
Vol 14 (12) ◽  
pp. 1333-1340 ◽  
Author(s):  
Cynthia F. Norton ◽  
Galen E. Jones
Keyword(s):  
Rhode Island ◽  
Culture Fluid ◽  
Chloride Ion ◽  
Water Soluble ◽  
Redox Activity ◽  
Blue Pigment ◽  
Ph Indicator ◽  
Ph Change ◽  
Deep Blue ◽  
Solid Media

A bacterium producing a deep blue pigment was isolated from Narragansett Bay, Rhode Island, and identified as Pseudomonas nigrifaciens. It requires at least 6.4 p.p.t. salinity for growth, 9.6 p.p.t. for pigmentation, and grows from 4 to 30 C, optimally at 15–18 C. An organic nitrogen source is necessary for growth but no specific amino acids or growth factors are required. Two major seawater ions are essential, potassium (10−3 M minimum, 8 × 10−3 M optimum) and magnesium (growth occurring in 1 week on 10−5 M, 10−2 M optimum at 20 h). Growth occurs without addition of sodium or chloride ion. Optimum pigmentation occurs on solid media, but pigmentation does occur in shaken vitamin-free casamino acids medium of 35 p.p.t. salinity. Water-soluble blue pigment appears during log phase and breaks down to a brownish coloration during stationary phase, coinciding with a pH change from 7.4 to 8.3. The blue pigment in sterile filtered culture fluid exhibits redox and pH indicator properties. Redox activity is observed in culture fluid, where standing causes reduction and shaking reoxidation.

scholarly journals New Blue Pigment Produced byPantoea agglomeransand Its Production Characteristics at Various Temperatures

2010 ◽  
Vol 77 (1) ◽  
pp. 172-178 ◽  
Author(s):  
Hiroshi Fujikawa ◽  
Ryo Akimoto
Keyword(s):  
Initial Density ◽  
Agar Plate ◽  
Water Soluble ◽  
Pigment Production ◽  
Blue Pigment ◽  
Bacterial Cells ◽  
Initial Cell ◽  
Low Temperature Storage ◽  
Deep Blue ◽  
Production Characteristics

ABSTRACTA bacterium capable of producing a deep blue pigment was isolated from the environment and identified asPantoea agglomerans. The pigment production characteristics of the bacterium under various conditions were studied. The optimal agar plate ingredients for pigment production by the bacterium were first studied: the optimal ingredients were 5 g/liter glucose, 10 g/liter tryptic soy broth, and 40 g/liter glycerol at pH 6.4. Bacterial cells grew on the agar plate during the incubation, while the pigment spread into the agar plate, meaning that it is water soluble. Pigment production was affected by the initial cell density. Namely, at higher initial cell densities ranging from 106.3to 108.2CFU/cm2on the agar plate, faster pigment production was observed, but no blue pigment was produced at a very high initial density of 109.1CFU/cm2. Thus, the cell population of 108.2CFU/cm2was used for subsequent study. Although the bacterium was capable of growing at temperatures above and below 10°C, it could produce the pigment only at temperatures of ≥10°C. Moreover, the pigment production was faster at higher temperatures in the range of 10 to 20°C. Pigment production at various temperature patterns was well described by a new logistic model. These results suggested that the bacterium could be used in the development of a microbial temperature indicator for the low-temperature-storage management of foods and clinical materials. To our knowledge, there is no otherP. agglomeransstrain capable of producing a blue pigment and the pigment is a new one of microbial origin.

scholarly journals Electronic and Assembly Properties of a Water-Soluble Blue Naphthalene Diimide

2021 ◽  
Author(s):  
Thomas Welsh ◽  
Olga Matsarskaia ◽  
Ralf Schweins ◽  
Emily Rose Draper
Keyword(s):  
Careful Consideration ◽  
Water Soluble ◽  
Mild Conditions ◽  
Deep Blue ◽  
Naphthalene Diimide

Herein we report on the synthesis and characterisation of a water soluble deep blue naphthalene diimide, (iPrNH)2NDI‒V. The synthesis is performed under mild conditions and careful consideration of the purification...

Purification and Characterization of Laccase fromChaetomium thermophilium and Its Role in Humification

1998 ◽  
Vol 64 (9) ◽  
pp. 3175-3179 ◽  
Author(s):  
Benny Chefetz ◽  
Yona Chen ◽  
Yitzhak Hadar
Keyword(s):  
Municipal Solid Waste ◽  
Solid Waste ◽  
Laccase Activity ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Water Soluble ◽  
Type I ◽  
Light Spectrum ◽  
Solid Media ◽  
Wide Range

ABSTRACT Chaetomium thermophilium was isolated from composting municipal solid waste during the thermophilic stage of the process.C. thermophilium, a cellulolytic fungus, exhibited laccase activity when it was grown at 45°C both in solid media and in liquid media. Laccase activity reached a peak after 24 h in liquid shake culture. Laccase was purified by ultrafiltration, anion-exchange chromatography, and affinity chromatography. The purified enzyme was identified as a glycoprotein with a molecular mass of 77 kDa and an isoelectric point of 5.1. The laccase was stable for 1 h at 70°C and had half-lives of 24 and 12 h at 40 and 50°C, respectively. The enzyme was stable at pH 5 to 10, and the optimum pH for enzyme activity was 6. The purified laccase efficiently catalyzed a wide range of phenolic substrates but not tyrosine. The highest levels of affinity were the levels of affinity to syringaldazine and hydroxyquinone. The UV-visible light spectrum of the purified laccase had a peak at 604 nm (i.e., Cu type I), and the activity was strongly inhibited by Cu-chelating agents. When the hydrophobic acid fraction (the humic fraction of the water-soluble organic matter obtained from municipal solid waste compost) was added to a reaction assay mixture containing laccase and guaiacol, polymerization took place and a soluble polymer was formed. C. thermophilium laccase, which is produced during the thermophilic stage of composting, can remain active for a long period of time at high temperatures and alkaline pH values, and we suggest that this enzyme is involved in the humification process during composting.

scholarly journals A Theoretical and Experimental Study of Electrochemical pH Control at Gold Interdigitated Microband Arrays

2021 ◽  
Author(s):  
Bernardo Patella ◽  
Robert Daly ◽  
Ian Seymour ◽  
Pierre Lovera ◽  
James Rohan ◽  
...  
Keyword(s):  
Electrode Array ◽  
Ph Control ◽  
Reduction Peak ◽  
Solution Ph ◽  
Ph Indicator ◽  
Ph Change ◽  
Initial Ph ◽  
The Impact ◽  
Strong Acids

In electroanalysis, solution pH is a critical parameter that often needs to be adjusted and controlled for the detection of particular analytes. This is most commonly performed by the addition of chemicals, such as strong acids or bases. Electrochemical in-situ pH control offers the possibility for the local adjustment of pH at the point of detection, without additional reagents. FEA simulations have been performed to guide experimental design for both electroanalysis and in-situ control of solution pH. No previous model exists that describes the generation of protons at an interdigitated electrode array in buffered solution with one comb acting as a protonator, and the other as the sensor. In this work, FEA models are developed to provide insight into the optimum conditions necessary for electrochemical pH control. The magnitude of applied galvanostatic current has a direct relation to the flux of protons generated and subsequent change in pH. Increasing the separation between the electrodes increases the time taken for protons to diffuse across the gap. The final pH achieved at both, protonators and sensor electrodes, after 1 second, was shown to be largely uninfluenced by the initial pH of the solution. The impact of buffer concentration was modelled and investigated. In practice, the pH at the electrode surface was probed by means of cyclic voltammetry, i.e., by cycling a gold electrode in solution and identifying the potential of the gold oxide reduction peak. A pH indicator, methyl red, was used to visualise the solution pH change at the electrodes, comparing well with the model’s prediction

scholarly journals The-Proof-of-Concept of Biochar Floating Cover Influence on Water pH

Water ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1802 ◽  
Author(s):  
Zhanibek Meiirkhanuly ◽  
Jacek A. Koziel ◽  
Andrzej Białowiec ◽  
Chumki Banik ◽  
Robert C. Brown
Keyword(s):  
Buffer Capacity ◽  
Corn Starch ◽  
Tap Water ◽  
Spatial And Temporal Variation ◽  
Gaseous Emissions ◽  
Inorganic Contaminants ◽  
Ph Indicator ◽  
Ph Change ◽  
Air Interface ◽  
Water Ph

Studies have shown that biochar has the potential to remove organic and inorganic contaminants from wastewater. pH is known to have a crucial role in the transformation of pollutants. In this research, we explore the feasibility of using biochars properties to control the pH near the water–air interface, so the gaseous emissions from water (or wastewater) could be mitigated. This study aimed to test the effects of a thin layer biochar addition on the spatial and temporal variation of water pH. Two types of biochar and water were tested. Highly alkaline porous (HAP; pH 9.2) biochars made of corn stover and red oak (RO; pH 7.5) were applied surficially to tap (pH 9.5) and deionized water (DI) (pH 5.4). The spatial pH of solutions was measured every 1 mm of depth on days 0, 2, and 4 after biochar application. The results showed that HAP biochar increased the pH of both tap and DI water, while RO decreased tap water pH and increased DI water pH. On day 0, there was no effect on tap water pH, while a pH change in DI water was observed due to its lower buffer capacity. In addition, the pH (temporal) migration from topically applied biochar into an aqueous solution was visualized using a colorimetric pH indicator and corn starch to increase viscosity (to prevent biochars from sinking). The results prove that the surficial application of biochar to water was able to change both the pH near the water–air interface and the pH of the solution with time. The pH change was dependent on the biochar pH and water buffer capacity. These results warrant further research into the floatability of biochars and into designing biochars with specific pH, which could be a factor influencing gaseous emissions from liquids that are sensitive to pH.

scholarly journals EXPERIMENTAL INVESTIGATION OF BIOGAS PRODUCTION USING FATTY WASTE/BIODUJŲ GAMYBOS EKSPERIMENTINIAI TYRIMAI PANAUDOJANT RIEBALINES ATLIEKAS/ЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ ПРОИЗВОДСТВА БИОГАЗА C ИСПОЛЬЗОВАНИЕМ ЖИРОВЫХ ОТХОДОВ

2008 ◽  
Vol 16 (4) ◽  
pp. 178-187 ◽  
Author(s):  
Pranas Baltrėnas ◽  
Mindaugas Kvasauskas
Keyword(s):  
Experimental Investigation ◽  
Sewage Sludge ◽  
Organic Waste ◽  
Biogas Production ◽  
Ph Indicator ◽  
Ph Change ◽  
Average Value ◽  
Temperature Mode ◽  
Biogas Output ◽  
Sulphuretted Hydrogen

The paper presents the findings of experimental investigation of biogas production using hen manure and sewage sludge with fatty waste (from margarine production). The following mixtures were made using this organic waste: hen manure and sewage sludge were mixed with fatty waste at 75% : 25%, 50% : 50% and 25% : 75% in volume. The paper analyses quantitative and qualitative composition of biogas: gas emission content, the concentrations of methane, sulphuretted hydrogen and oxygen in it as well as temperature and pH change during the experiment. Mixing hen manure with fatty waste at 75% : 25% and maintaining psychrophilic temperature mode, the highest biogas output (0.09 m3) from 0.2 m3 substrate in 32 days was recorded. When using the mixture of sewage sludge and fatty waste at 75% : 25% and maintaining psychrophilic temperature mode, the highest biogas output (0.10 m) from 0.2 m substrate in 32 days was recorded. When using these mixtures, the concentration of methane was also the highest, and the average values reached 58.5% (with hen manure) and 61.7% (with sewage sludge). The evaluation of gas emissions and methane concentration in gas shows that the most efficient use of biogas could be obtained using biogas emitted from the mixture of hen manure and sewage sludge with fatty waste mixed at 75% : 25%. The concentration of sulphuretted hydrogen in biogas did not exceed 3%, the concentration of oxygen decreased from 4.9 % to 1.8%. pH indicator was decreasing when more fatty waste was added to the mixture. When using hen manure, the average value of pH changed from 6.4 to 6.3, when using sewage sludge ‐ from 6.6 to 6.3. Santrauka Pateikiami eksperimentinių biodujų gamybos tyrimų, naudojant vištų mėšlą ir nuotekų valymo dumblą su riebalinėmis (margarino gamybos) atliekomis, rezultatai. Iš šių organinių atliekų buvo paruošti mišiniai: vištų mėšlas bei nuotekų valymo dumblas buvo maišomas su riebalinėmis atliekomis 75 % : 25 %, 50 % : 50 % ir 25 % : 75 %. Darbe nagrinėjama biodujų kiekybinė ir kokybinė sudėtis: išsiskyrusių dujų kiekis, metano, sieros vandenilio ir deguonies koncentracija jose, temperatūros bei pH pokytis eksperimento metu. Eksperimentų metu bioreaktoriuose buvo palaikomas psichrofilinis temperatūrinis režimas. Nustatyta, kad didžiausias biodujų kiekis (0,09 m3 ) išsiskiria vištų mėšlą maišant su riebalinėmis atliekomis santykiu 75 % : 25 %. Naudojant nuotekų valymo dumblo ir riebalinių atliekų mišinį (75 % : 25 %), taip pat gauta didžiausia biodujų išeiga per visą eksperimento laikotarpį – 0,10 m3 . Metano koncentracija naudojant šiuos mišinius taip pat buvo didžiausia. Vidutinė reikšmė siekė 58,5 % (su vištų mėšlu) bei 61,7 % (su nuotekų dumblu). Įvertinus išsiskyrusių dujų kiekį ir metano koncentraciją jose, nustatyta, kad efektyviausiai būtų galima naudoti biodujas, išsiskyrusias iš vištų mėšlo ir nuotekų valymo dumblo su riebalinėmis atliekomis, kurių santykis 75 % : 25 %. Sieros vandenilio koncentracija biodujose neviršijo 3 %, deguonies koncentracija mažėjo nuo 4,9 % iki 1,8 %. pH rodiklis mažėjo į biomasės mišinį įdedant daugiau riebalinių atliekų. Naudojant vištų mėšlą vidutinė pH reikšmė kito nuo 6,4 iki 6,3, naudojant nuotekų valymo dumblą – nuo 6,6 iki 6,3. Резюме Представлены результаты экспериментальных исследований по производству биогаза с использованием куриного помета и ила сточных вод с жировыми отходами (от производства маргарина). Были исследованы следующие смеси из органических отходов: куриный помет и ил сточных вод были смешаны с жировыми отходами в объеме 75% : 25%, 50% : 50% и 25% : 75%. Проанализирован количественный и качественный состав биогаза: газовое содержание эмиссии, концентрации метана, сероводорода и кислорода, а также изменение температуры и фактора pH в течение эксперимента. Во время эксперимента в биореакторе поддерживался психрофильный температурный режим. Было установлено, что при смешивании куриного помета с жировыми отходами в соотношении 75% : 25% и поддерживании психрофильного температурного режима наибольший выпуск биогаза (от 0,09 м3 до 0,2 м3 субстрата) обнаружен спустя 32 дня. При использовании смеси из ила сточных вод и жировых отходов в соотношении 75% : 25% и поддерживании психрофильного температурного режима был установлен наибольший выпуск биогаза (от 0,10 м3 до 0,2 м3 субстрата) спустя 32 дня. При использовании этих смесей концентрация метана также была самая большая, ее средние значения составляли 58,5% (с куриным пометом) и 61,7% (с илом сточных вод). В результате оценки газовой эмиссии и концентрации метана в эмиссии установлено, что наиболее эффективным будет использование биогаза, получаемого от смеси куриного помета и ила сточных вод с жировыми отходами в соотношении 75% : 25%. Концентрация сероводорода в биогазе не превышала 3%, концентрация кислорода уменьшилась от 4,9% до 1,8%. Индикатор фактора pH уменьшался, когда к смеси добавлялось больше жировых отходов. При использовании только куриного помета среднее значение фактора pH менялось от 6,4 до 6,3, при использовании ила сточных вод – от 6,6 до 6,3.

Protolytic Reactions in Photosystem II: a New Model for the Release of Protons Accompanying the Photooxidation of Water

1977 ◽  
Vol 32 (7-8) ◽  
pp. 617-626 ◽  
Author(s):  
Satham Saphon ◽  
Antony R. Crofts
Keyword(s):  
Electron Acceptor ◽  
Neutral Red ◽  
Glass Electrode ◽  
Ph Indicator ◽  
Ph Change ◽  
Benzyl Viologen ◽  
Ph Changes ◽  
The Third ◽  
Cresol Red ◽  
Indicator Dye

Using pH indicator dye techniques we have investigated the pH changes in dark-adapted chloro- plasts following excitation by short flashes. Two types of pH indicator, cresol red and neutral red, were used, to follow the pH changes either inside or outside the thylakoids, or the net change when the membrane was made permeable to protons by uncoupling agents. (1)With cresol red which showed the net pH changes inside and outside the thylakoids, an oscillation of the flash yield of H+ occurred with a periodicity of 4 (minima on the first and fifth flashes, the yield on the third being not significantly different from the yields on the second and fourth flashes). The pH changes did not occur in synchrony with O2-evolution. (2)The net flash yields without addition of electron acceptor were similar to those with benzyl- viologen. The results were comparable with those obtained with the glass electrode technique by Fowler and Kok (C. F. Fowler and B. Kok, Biochim. Biophys. Acta 357, 299 - 307 [1974]). (3)The net flash yields with ferricyanide as electron acceptor of photosystem I were higher than those in the absence of acceptor, or with benzylviologen. On the first and fifth flashes a net acidification was always observed. (4)In the presence of 3- (3,4-dichlorphenyl) -1,1-dimethylurea (DCMU) a rapid acidification also occured on the first flash, while the pH changes induced by subsequent flashes were inhibited. (5)The uncoupler methylamine did not inhibit the proton uptake outside the thylakoids. (6)With neutral red as indicator for the net pH change inside and outside the thylakoids, the same oscillation of the flash yield occured as with cresol red. (7)With neutral red in the precense of an external buffer, as a pH indicator for the internal aqueous phase alone, an oscillation of the flash yield with a periodicity of 4 also occured. The first and second flash yields were higher compared with the third than the equivalent yields of oxygen. (8)We discuss the results with respect to a model for the release of protons in the water- splitting enzyme reactions, in which protons are not released in synchrony with O2 , but in the transitions of all the states of the watersplitting enzyme with the exception of S1 → S2 . Our results are consistent with this model when account is taken of the release of protons inside the thylakoids with a periodicity of 2, associated with electron transfer from reduced plastoquinone.

Water soluble porphyrin-fluorescein triads: Design, DFT calculation and pH-change-triggered fluorescence response

2020 ◽  
Vol 402 ◽  
pp. 112832
Author(s):  
Dmitriy A. Lazovskiy ◽  
Galina M. Mamardashvili ◽  
Il’ya A. Khodov ◽  
Nugzar Z. Mamardashvili
Keyword(s):  
Dft Calculation ◽  
Water Soluble ◽  
Ph Change ◽  
Fluorescence Response ◽  
Water Soluble Porphyrin

scholarly journals A New Water-Soluble Near-Neutral Ratiometric Fluorescent pH Indicator

2008 ◽  
Author(s):  
Sheng Yao ◽  
Katherine J. Schafer-Hales ◽  
Kevin D. Belfield
Keyword(s):  
Water Soluble ◽  
Ph Indicator

scholarly journals Immunocytochemical localization of protein antigens in large sections of tissues embedded in water-soluble embedding media.

1986 ◽  
Vol 34 (4) ◽  
pp. 535-538 ◽  
Author(s):  
T M Duello ◽  
F C Gumkowski
Keyword(s):  
Water Soluble ◽  
Placental Lactogen ◽  
Protein Antigens ◽  
Ph Change ◽  
Binucleate Cells ◽  
Tissue Sections ◽  
Pre Treatment ◽  
Ethanol Pretreatment ◽  
Large Sections ◽  
Absolute Etoh

JB4 and Immunobed are water-soluble embedding media used for embedding large blocks of tissue. Immunobed was specifically designed for immunocytochemistry because ethanol extraction of an additive in the monomer of the resin is reported to render tissue sections permeable to immunoglobulins. We have modified the manufacturer's protocol to accomplish localization of two protein antigens in tissues embedded in either JB4 or Immunobed. Luteinizing hormone-beta (LH beta) was localized in sections of rat and bovine pituitary tissues and bovine placental lactogen (bPL) was localized in sections of placentomes from bovine placentas. Sections received one of the following pre-treatments: absolute EtOH; NaHCO3 buffer, pH 6-10; EtOH followed by NaHCO3 buffer; one of several enzymes; EtOH followed by enzyme; NaHCO3 buffer followed by enzyme. Anti-LH beta stained only pituitary gonadotrophs and anti-bPL stained only placental binucleate cells, as assessed by absorption controls. Pre-treatment with enzyme was required for staining of sections, but an alkaline pH change (NaHCO3) had little or no effect. Ethanol pretreatment had little or no effect alone or in conjunction with NaHCO3 or enzyme. Sections were sufficiently thin (1.5 micron) to afford resolution of structure, but suitably large (approximately 2 cm2) to minimize problems of sampling.

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