Exigences en sels minéraux de Corynebacterium sepedonicum (Spiek. & Kott.) Skapt. & Burkh.

1968 ◽  
Vol 14 (4) ◽  
pp. 467-473
Author(s):  
Lucien M. Bordeleau ◽  
Robert-A. Lachance
Keyword(s):  
Amino Acids ◽  
Potassium Phosphate ◽  
Ammonium Phosphate ◽  
Tris Buffer ◽  
Good Growth ◽  
Free Medium ◽  
Corynebacterium Sepedonicum ◽  
Potassium Phosphate Monobasic ◽  
Nickel Cobalt ◽  
Potassium Phosphate Dibasic

The mineral requirements of Corynebacterium sepedonicum (Spiek. & Kott.) Skapt. & Burkh., were studied in a medium containing seven amino acids, three vitamins, dextrose, and tris buffer. The organism does not grow in a mineral-free medium. Potassium phosphate monobasic and potassium phosphate dibasic are required in concentrations of 1.2 × 10−3 and 2.8 × 10−3 mole per liter respectively. Ammonium phosphate monobasic inhibits the growth. Potassium can be replaced by calcium when H3PO4 is used as a source of PO4---. Magnesium as MgCl2∙6H2O in concentration of 2 × 10−3 mole per liter is required for good growth but does not affect the morphology of the organism as reported for other bacteria. Manganese as MnSO4>∙H2O and iron as Fe(SO4)2(NH4)2∙6H2O, in concentrations of 10−3 and 10−6 mole per liter respectively, are not essential but stimulate the growth when added to potassium phosphate (KH2PO4 and K2HPO4) and magnesium. Copper, nickel, cobalt, aluminum, and zinc were found to be inhibitory.

Des acides aminés stimulateurs et inhibiteurs à la croissance de Corynebacterium sepedonicum (Spieck. et Kott., Skapt. et Burkh.) dans des milieux synthétiques

1978 ◽  
Vol 24 (9) ◽  
pp. 1087-1092
Author(s):  
G. J. Ikin ◽  
H. J. Hope ◽  
R. A. Lachance
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Ammonium Phosphate ◽  
Methionine Sulfoxide ◽  
High Rate ◽  
Molar Ratio ◽  
Corynebacterium Sepedonicum ◽  
Ring Rot ◽  
Acides Aminés ◽  
Synthetic Media

Some aspects of the growth and amino acid metabolism of Corynebacterium sepedonicum, the organism responsible for potato ring rot, have been studied in synthetic media. It has been demonstrated that organic sulfur is required for growth. Methionine supports growth and can be replaced by methionine sulfoxide and cystathionine. Methionine is a micrometabolite for this species as indicated by the fact that optimum growth can be obtained in an asparagines–methionine (asn-met) containing medium when the molar ratio of these amino acids is 56:1. Increasing the proportion of methionine does not increase the growth. Both asparagine and glutamine are metabolized very quickly and provide for equivalent rapid growth unlike aspartic and glutamic acids. In the case of the last two amino acids, growth can be increased if dibasic ammonium phosphate is added to the medium although this compound alone will not support growth in the culture medium. The intracellular soluble asparagine level is extremely low in cells from the asn-met medium indicating a high rate of metabolism compared to aspartic acid. Cystine and cysteine were found to be inhibitory to the organism: they do not affect the rate of uptake of asn or met but do alter the organism's metabolism as reflected by changes in the free amino acid pool. The concentrations of cystine and cysteine required for measurable inhibition are much higher than those found in soluble amino acids of potato tubers.

In vitro production of struvite by Bacillus pumilus

1991 ◽  
Vol 37 (12) ◽  
pp. 978-983 ◽  
Author(s):  
Berlin Nelson ◽  
James Struble ◽  
Gregory McCarthy
Keyword(s):  
Bacillus Pumilus ◽  
Potassium Phosphate ◽  
Ammonium Phosphate ◽  
Maximum Size ◽  
In Vitro Production ◽  
Magnesium Ammonium Phosphate ◽  
X Ray ◽  
Magnesium Ammonium ◽  
Vitro Production

A strain of Bacillus pumilus, isolated from the soilborne sclerotia of a fungus, produced crystals of the mineral struvite (magnesium ammonium phosphate hexahydrate) on Difco nutrient agar and a yeast extract agar containing magnesium sulphate and potassium phosphate. Crystals were macroscopically observed after 6–20 days of bacterial growth and reached a maximum size of 3 × 0.5 mm. Crystals were identified by X-ray powder diffractometry. These findings support the hypothesis that bacteria are involved in the biogenic formation of struvite in nature. Key words: struvite, magnesium ammonium phosphate hexahydrate, Bacillus pumilus, biomineral.

Some aspects of the nitrogen metabolism of Nodularia spumigena (Cyanophyceae)

1974 ◽  
Vol 52 (4) ◽  
pp. 719-726 ◽  
Author(s):  
E. L. Camm ◽  
J. R. Stein
Keyword(s):  
Amino Acids ◽  
Growth Rate ◽  
Growth Inhibition ◽  
Glutamic Acid ◽  
Nitrogen Metabolism ◽  
Free Medium ◽  
Nodularia Spumigena ◽  
Whole Cells ◽  
Acid Dehydrogenase ◽  
Reducing Ability

Nitrate-reducing ability, NO2−-reducing ability, and glutamic acid dehydrogenase levels were measured in two clones of Nodularia spumigena Mertens. Measurements with whole cells of both clones show that NO3− reduction is stimulated by NO3−, and that NO2− reduction is probably stimulated by NO2−. The NO3−-reducing system is stimulated by light and inhibited by NH4+. These controls and possible control over NH4+ incorporation into amino acids are compared to systems operative in other organisms.Differences in growth and physiology of the clones in growth rate on N-free medium, growth inhibition by urea, and NO2− accumulation in the medium are discussed.

Platanus Fruit-Like Nickel Cobalt Ammonium Phosphate/MWCNTs Composite Grown on Nickel Foam for High-Performance Supercapacitors

NANO ◽  
2020 ◽  
Vol 15 (04) ◽  
pp. 2050044 ◽  
Author(s):  
Yong Zhang ◽  
Han-Xin Mei ◽  
Jing Yang ◽  
Shi-Wen Wang ◽  
Hai-Li Gao ◽  
...  
Keyword(s):  
High Performance ◽  
Raw Materials ◽  
Nickel Foam ◽  
Specific Capacity ◽  
Ammonium Phosphate ◽  
The Other ◽  
Structure And Properties ◽  
Nickel Cobalt ◽  
Target Sample ◽  
The Ideal

Platanus fruit-like nickel cobalt ammonium phosphate/MWCNTs composites for high-performance supercapacitors were successfully prepared by a two-step hydrothermal method using NiSO[Formula: see text]6H2O, CoSO[Formula: see text]7H2O, (NH4)H2PO4 and MWCNTs as raw materials, respectively. The effects of MWCNTs addition on the structure and properties of the composites were investigated. The results showed that when the doped amount of MWCNTs is 7.3[Formula: see text]wt.%, the as-prepared NCAP/M7.3 composite presented an obvious platanus fruit likeness, its surface is rough and porous with a diameter of 3–5[Formula: see text][Formula: see text]m. Compared with the other MWCNTs contents, the NCAP/M7.3 electrode obtained under this condition showed the best capacitive performance. The discharge specific capacity was up to 1270[Formula: see text]F[Formula: see text]g[Formula: see text] at current density of 1[Formula: see text]A[Formula: see text]g[Formula: see text], which remains to be 902[Formula: see text]F[Formula: see text]g[Formula: see text] even at a high density of 10[Formula: see text]A[Formula: see text]g[Formula: see text]. The results show that the target sample can be the ideal electrode material for supercapacitor.

INHIBITION OF GROWTH OF PSEUDOMONAS DENITRIFICANS BY AMINO ACIDS

1966 ◽  
Vol 12 (6) ◽  
pp. 1095-1098 ◽  
Author(s):  
Horace J. Daniels
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Basal Medium ◽  
Ammonium Phosphate ◽  
Complete Medium ◽  
Mineral Salts ◽  
Culture Studies ◽  
Pseudomonas Denitrificans ◽  
Inhibition Of Growth ◽  
Amino Acid Mixtures

A large number of amino acids failed to support growth of Pseudomonas denitrificans in a basal medium composed of glucose, ammonium phosphate, and other mineral salts. Inability of an amino acid to support growth correlated well with its inhibitory action in a complete medium made up by adding L-glutamic acid to the basal medium. D-Amino acids were more toxic than the corresponding L-forms, and neutral amino acids were more toxic than acidic amino acids. Basic amino acids which were least toxic supported the best growth. The danger of the indiscriminate use of amino acid mixtures for culture studies is discussed.

Effets des purines et des pyrimidines sur la croissance de Corynebacterium sepedonicum (Spiek. & Kott.) Skapt. & Burkh.

1968 ◽  
Vol 14 (4) ◽  
pp. 475-478 ◽  
Author(s):  
Lucien M. Bordeleau ◽  
Robert-A. Lachance
Keyword(s):  
Amino Acids ◽  
Nucleic Acids ◽  
Synthetic Medium ◽  
Limiting Factor ◽  
Mineral Salts ◽  
Corynebacterium Sepedonicum ◽  
Purines And Pyrimidines

The effects of purines and pyrimidines on the growth of Corynebacterium sepedonicum were studied in a synthetic medium containing amino acids, vitamins, mineral salts, and glucose. Among four purines tested, only adenine inhibited the growth of the organism. Hypoxanthine and guanine reversed the inhibition caused by adenine. None of four pyrimidines tested affected the growth. In the presence of pyrimidines, only hypoxanthine reversed the inhibition caused by adenine. It appears that purines and pyrimidines do not stimulate the growth of C. sepedonicum and that the synthesis of nucleic acids from these bases is not a limiting factor in the growth of the organism.

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