A RAPID MICRO TISSUE CULTURE ASSAY IN BS-C-1 CELLS FOR THE TITRATION AND NEUTRALIZATION OF MEASLES VIRUS

1967 ◽  
Vol 13 (3) ◽  
pp. 313-319 ◽  
Author(s):  
Pierre Moreau ◽  
John Furesz
Keyword(s):  
Tissue Culture ◽  
Measles Virus ◽  
Constant Pressure ◽  
Fetal Bovine Serum ◽  
Mineral Oil ◽  
Tissue Cultures ◽  
Kidney Cell Line ◽  
Virus Titration ◽  
Inverted Microscope ◽  
Fetal Bovine

A simple, rapid and reproducible micro tissue culture assay in the BS-C-1 (Cercopithecus monkey kidney) cell line was developed for the titration and neutralization of measles virus. Serial dilutions of virus (or serum) were made with spiral loops in disposable microplates and 8 × 103 BS-C-1 cells suspended in 0.05 ml of medium 199 with 2% fetal bovine serum were added to each dilution. After the addition of 0.1 ml of sterile mineral oil to each microculture, the plates were covered with a plexiglass sheet and placed in a humidified incubator (37 °C) under a constant pressure of 5% CO2 for S days. Micro tissue cultures were examined with the aid of an inverted microscope for viral cytopathic changes on the fourth and fifth day.The micro assay was applied to the virus titration of various live, attenuated measles vaccines, as well as to the antibody titration of human and animal sera following vaccination with either live or inactivated measles vaccines.

A micro tissue culture test for the titration and neutralization of rubella virus

1969 ◽  
Vol 15 (1) ◽  
pp. 67-71 ◽  
Author(s):  
John Furesz ◽  
Pierre Moreau ◽  
Walter Yarosh
Keyword(s):  
Tissue Culture ◽  
Rubella Virus ◽  
Fetal Calf Serum ◽  
Constant Pressure ◽  
Calf Serum ◽  
Rabbit Kidney ◽  
Tissue Cultures ◽  
Virus Titration ◽  
One Step ◽  
Microneutralization Test

A simple and reproducible micro tissue culture assay has been devised in RK13 and LLC-RK1 rabbit kidney cells for the titration and neutralization of rubella virus. In this "one-step" assay all virus and serum dilutions were prepared with spiral loops in disposable microplates and tissue cultures suspended in medium 199 and 3% horse or fetal calf serum were added to the microcups simultaneously. Micro tissue cultures were kept in a humidified incubator (36 °C) under a constant pressure of 5% CO2 for 8 days and were read microscopically for viral cytopathic changes on the seventh and eighth day. The microneutralization test performed in LLC-RK1 cell cultures was shown to be a reliable method for the detection of small amounts of rubella antibodies in human sera.The micro assay may be also applied to the virus titration of live, attenuated rubella vaccines.

Fetal bovine serum and other sera used in tissue culture increase epithelial permeability

1993 ◽  
Vol 29 (3) ◽  
pp. 235-238 ◽  
Author(s):  
Kathleen H. Mortell ◽  
Alan D. Marmorstein ◽  
Eva B. Cramer
Keyword(s):  
Tissue Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Epithelial Permeability ◽  
Fetal Bovine

Binding of mycoplasmas to solid phase adsorbents

2005 ◽  
Vol 53 (3) ◽  
pp. 299-307 ◽  
Author(s):  
Susan Szathmáry ◽  
Nandani Rajapakse ◽  
Ibolya Székely ◽  
E. Pitlik ◽  
Judit Bíró ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Adhesion Molecules ◽  
Bovine Serum ◽  
Solid Phase ◽  
Fetal Bovine Serum ◽  
Vaccine Production ◽  
Fetal Bovine ◽  
Phosphate Buffered Saline ◽  
Lipid Structures

The capture of mycoplasmas (M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticum and M. arthritidis) based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, and for concentrating mycoplasmas in vaccine production.

scholarly journals Особливості клітинного циклу мезенхімальних стовбурових клітин з жирової тканини собаки за різних пасажів культивування

2017 ◽  
Vol 19 (78) ◽  
pp. 36-40
Author(s):  
L.V. Kladnytska
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Culture Medium ◽  
Fetal Bovine Serum ◽  
Inverted Microscope ◽  
Proliferative Pool ◽  
Fetal Bovine ◽  
Diploid Cells

The features of the cell cycle of culture of adipose-derived mesenchymal stem cells from the for different cultivating passages were studied. Mesenchymal stem cells were obtained from the adipose tissue of the dog under a laminar flow hood by an explant method in our modification. Cell cultivation was carried out at 37 °C, 100% moisture and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic. The culture medium was changed 2–3 times per week and the cells were selected by their capacity to attach to the flask surface. When culture flasks became 80% confluence, cells were detached with 0.25% trypsin containing 1 mmol/L EDTA and subsequently replayed at a concentration of 104 cells/cm2 for next passaging. A cells culture of adipose derived mesenchymal stem cells was obtained on the 2nd, 7th and 12th passages. The method of flow cytometry determined the level of aneuploid cells and the distribution in the cell cycle phases. The morphology of cells of different passages was studied using an inverted microscope Axiovert 40. It was investigated that the culture of mesenchymal stem cells from adipose tissue in the 2nd passage contains a significant number of the proliferative pool (S + G2/M) cells and it was 29.51 ± 3.56% of the total number of diploid cells. The number of aneuploid cells was 1.55 ± 0.43%. All cells had fibroblast-like morphology. It was established that in the middle passages (7th) in the culture of mesenchymal stem cells from the adipose tissue of the dog no significant changes were found in the distribution of cells in the phases of the cell cycle. The number of diploid cells of the proliferative pool S + G2/M and the G0/G1 pre-synthetic period remains unchanged. The level of aneuploidy increases only within the tendency. Morphologically, cells had fibroblast-like form. It was determined on 12th passage of cultivation, a significant decrease in the number of cells of the proliferative pool (S+G2/M), which was 18.93 ± 0.66% of the total number of diploid cells compared to the 2nd passage. The number of aneuploid cells increased and it was 3.49 ± 0.38%. Morphologically, separate cells had processes. The indicator of the effect of cells cultivation on the content of diploid cells of the proliferative pool (S+G2/M) in culture is ɳ2x = 70% (P < 0.05). So, first characteristic properties of the aging of the culture of canine adipose-derived mesenchymal stem cells appear on the 12th passage of cultivation.

Effect of fractions of horse and fetal bovine serum on neoplastic conversion of C3H mouse cells in tissue culture

In Vitro ◽  
1971 ◽  
Vol 6 (6) ◽  
pp. 437-440 ◽  
Author(s):  
Floyd M. Price ◽  
R. Raymond Gantt ◽  
Virginia J. Evans
Keyword(s):  
Tissue Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Mouse Cells ◽  
Fetal Bovine

scholarly journals The humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture

2004 ◽  
Vol 18 (1) ◽  
pp. 1-12 ◽  
Author(s):  
J. van der Valk ◽  
D. Mellor ◽  
R. Brands ◽  
R. Fischer ◽  
F. Gruber ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Free Cell ◽  
Serum Free ◽  
Cell And Tissue Culture ◽  
Fetal Bovine

In vitro cultivation of hemocytes of Malacosoma disstria Hübner (Lepidoptera: Lasiocampidae)

1971 ◽  
Vol 49 (10) ◽  
pp. 1355-1358 ◽  
Author(s):  
S. S. Sohi
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Fetal Bovine Serum ◽  
Primary Cultures ◽  
Malacosoma Disstria ◽  
In Vitro Cultivation ◽  
Original Source ◽  
Insect Tissue Culture ◽  
Fetal Bovine

Prolonged culturing of the hemocytes of Malacosoma disstria has been accomplished using Grace's insect tissue culture medium supplemented with fetal bovine serum (5%) and Bombyx mori hemolymph (3%). The cultures started to grow after 3–6 months. These cells have now been in vitro for over 16 months, and have been subcultured 35 times. Three types of cells were present in primary cultures, but only one type, prohemocytes, persisted and grew after subculturing. The M. disstria larvae that were used as the original source of hemocytes were naturally infected with the microsporidian Glugea disstriae. The microsporidian also grew in [he cell cultures, and the cells are still infected.

scholarly journals Fortified Sera and Their Use in Environmental Virology

2000 ◽  
Vol 66 (5) ◽  
pp. 2259-2262 ◽  
Author(s):  
Jonathan L. Hoyt ◽  
Aaron B. Margolin
Keyword(s):  
Kidney Cell ◽  
Fetal Bovine Serum ◽  
Most Probable Number ◽  
Kidney Cell Line ◽  
Probable Number ◽  
Environmental Virology ◽  
Green Monkey ◽  
Fetal Bovine ◽  
Wastewater Samples ◽  
Virus Strains

ABSTRACT Four commercially available fortified sera were compared to fetal bovine serum (FBS) with regard to their ability to maintain or increase the sensitivity of the Buffalo green monkey (BGM) kidney cell line to viral infection. Nine virus strains and five wastewater samples were used. Fortified sera were comparable to FBS for the enumeration of some viruses by the plaque method and for the detection of virus in wastewater by the most-probable-number assay.

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