BACTERIAL METABOLISM OFD-ASPARTATE INVOLVING RACEMIZATION AND DECARBOXYLATION

1966 ◽  
Vol 12 (4) ◽  
pp. 745-751 ◽  
Author(s):  
A. J. Markovetz ◽  
W. J. Cook ◽  
A. D. Larson
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Bacterial Metabolism ◽  
Alanine Racemase ◽  
Whole Cells

A pseudomonad was isolated from soil with D-aspartate serving as sole carbon source. Whole cells and cellular extracts produced carbon dioxide and DL-alanine from D- and L-aspartate. The addition of oxamycin to cellular extracts inhibited the alanine racemase found to be present and it was ascertained that L-alanine was formed from both D- and L-aspartate, indicating that D-aspartate had been racemized to the L-isomer with subsequent decarboxylation to L-alanine.

scholarly journals Genome Sequence of Carbon Dioxide-Sequestering Serratia sp. Strain ISTD04 Isolated from Marble Mining Rocks

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Manish Kumar ◽  
Rajesh Kumar Gazara ◽  
Sandhya Verma ◽  
Madan Kumar ◽  
Praveen Kumar Verma ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sodium Bicarbonate ◽  
Genome Sequence ◽  
Sole Carbon Source

The Serratia sp. strain ISTD04 has been identified as a carbon dioxide (CO 2 )-sequestering bacterium isolated from marble mining rocks in the Umra area, Rajasthan, India. This strain grows chemolithotrophically on media that contain sodium bicarbonate (NaHCO 3 ) as the sole carbon source. Here, we report the genome sequence of 5.07 Mb Serratia sp. ISTD04.

β-Xylosidases in the yeast Cryptococcus albidus var. aerius

1976 ◽  
Vol 22 (2) ◽  
pp. 312-315 ◽  
Author(s):  
V. Notario ◽  
T. G. Villa ◽  
J. R. Villanueva
Keyword(s):  
Carbon Source ◽  
Cell Walls ◽  
Acid Treatment ◽  
Sole Carbon Source ◽  
Total Activity ◽  
Periplasmic Space ◽  
Cryptococcus Albidus ◽  
Whole Cells ◽  
Mild Acid ◽  
Xylosidase Activity

β-Xylosidase activity has been detected in cell-free extracts and in culture fluids when Cryptococcus albidus var. aerius was grown on glucose as the sole carbon source. The enzyme appears to be constitutive. Mild acid treatment of whole cells suggested that the total activity is located in the periplasmic space and some experiments indicated that it is partially associated with the cell walls. DEAE-Sephadex A50 chromatography has shown that there are two different forms of β-xylosidase in the cell-free extracts, but only one form is present in the supernatants of culture.

scholarly journals Bacterial metabolism of 2,4-dichlorophenoxyacetate

1971 ◽  
Vol 122 (4) ◽  
pp. 543-551 ◽  
Author(s):  
W. C. Evans ◽  
B. S. W. Smith ◽  
H. N. Fernley ◽  
J. I. Davies
Keyword(s):  
Carbon Source ◽  
Liquid Medium ◽  
Aromatic Ring ◽  
Sole Carbon Source ◽  
Major Metabolite ◽  
Bacterial Metabolism ◽  
Mineral Salts ◽  
Ring Fission ◽  
Unstable Compound

1. Two Pseudomonas strains isolated from soil metabolized 2,4-dichlorophenoxyacetate (2,4-D) as sole carbon source in mineral salts liquid medium. 2. 2,4-Dichlorophenoxyacetate cultures of Pseudomonas I (Smith, 1954) contained 2,4-dichlorophenol, 2-chlorophenol, 3,5-dichlorocatechol and α-chloromuconate, the last as a major metabolite. 3. Dechlorination at the 4(p)-position of the aromatic ring must therefore take place at some stages before ring fission. 4. Pseudomonas N.C.I.B. 9340 (Gaunt, 1962) cultures metabolizing 2,4-dichlorophenoxyacetate contained 2,4-dichloro-6-hydroxyphenoxyacetate, 2,4-dichlorophenol, 3,5-dichlorocatechol and an unstable compound, probably αγ-dichloromuconate. 5. Cell-free extracts of the latter organism grown in 2,4-dichlorophenoxyacetate cultures contained an oxygenase that converted 3,5-dichlorocatechol into αγ-dichloromuconate, a chlorolactonase that in the presence of Mn2+ ions converted the dichloromuconate into γ-carboxymethylene-α-chloro-Δαβ-butenolide, and a delactonizing enzyme that gave α-chloromaleylacetate from this lactone. 6. Pathways of metabolism of 2,4-dichlorophenoxyacetate are discussed.

Microbial electrosynthesis of butyrate from carbon dioxide

2015 ◽  
Vol 51 (15) ◽  
pp. 3235-3238 ◽  
Author(s):  
R. Ganigué ◽  
S. Puig ◽  
P. Batlle-Vilanova ◽  
M. D. Balaguer ◽  
J. Colprim
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Microbial Electrosynthesis ◽  
First Time

This work proves for the first time the bioelectrochemical production of butyrate from CO2as a sole carbon source.

A Flavobacterium sp. that degrades diazinon and parathion

1973 ◽  
Vol 19 (7) ◽  
pp. 873-875 ◽  
Author(s):  
N. Sethunathan ◽  
T. Yoshida
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Mineral Medium ◽  
Paddy Water

A Flavobacterium sp., isolated from paddy water by enrichment culture technique, decomposed diazinon in a mineral medium as sole carbon source. The bacterium readily hydrolyzed diazinon to 2-isopropyl-6-methyl-4-hydroxy-pyrimidine which was then converted to carbon dioxide. The bacterium also converted parathion to p-nitrophenol. The enzyme involved in the hydrolysis was constitutive.

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp

scholarly journals Assessment of Voltage Influence in Carbon Dioxide Fixation Process by a Photo-Bioelectrochemical System under Photoheterotrophy

2021 ◽  
Vol 9 (3) ◽  
pp. 474
Author(s):  
Sara Díaz-Rullo Edreira ◽  
Silvia Barba ◽  
Ioanna A. Vasiliadou ◽  
Raúl Molina ◽  
Juan Antonio Melero ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Future Development ◽  
Metabolic Pathways ◽  
Co2 Fixation ◽  
Oxygen Demand ◽  
Carbon Uptake ◽  
Bioelectrochemical System ◽  
Electron Sink ◽  
Soluble Carbon

Bioelectrochemical systems are a promising technology capable of reducing CO2 emissions, a renewable carbon source, using electroactive microorganisms for this purpose. Purple Phototrophic Bacteria (PPB) use their versatile metabolism to uptake external electrons from an electrode to fix CO2. In this work, the effect of the voltage (from −0.2 to −0.8 V vs. Ag/AgCl) on the metabolic CO2 fixation of a mixed culture of PPB under photoheterotrophic conditions during the oxidation of a biodegradable carbon source is demonstrated. The minimum voltage to fix CO2 was between −0.2 and −0.4 V. The Calvin–Benson–Bassham (CBB) cycle is the main electron sink at these voltages. However, lower voltages caused the decrease in the current intensity, reaching a minimum at −0.8 V (−4.75 mA). There was also a significant relationship between the soluble carbon uptake in terms of chemical oxygen demand and the electron consumption for the experiments performed at −0.6 and −0.8 V. These results indicate that the CBB cycle is not the only electron sink and some photoheterotrophic metabolic pathways are also being affected under electrochemical conditions. This behavior has not been tested before in photoheterotrophic conditions and paves the way for the future development of photobioelectrochemical systems under heterotrophic conditions.

scholarly journals Study of a plugging microbial consortium using crude oil as sole carbon source

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Microbial Consortium
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