Microbial electrosynthesis of butyrate from carbon dioxide

2015 ◽  
Vol 51 (15) ◽  
pp. 3235-3238 ◽  
Author(s):  
R. Ganigué ◽  
S. Puig ◽  
P. Batlle-Vilanova ◽  
M. D. Balaguer ◽  
J. Colprim
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Microbial Electrosynthesis ◽  
First Time

This work proves for the first time the bioelectrochemical production of butyrate from CO2as a sole carbon source.

scholarly journals Genome Sequence of Carbon Dioxide-Sequestering Serratia sp. Strain ISTD04 Isolated from Marble Mining Rocks

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Manish Kumar ◽  
Rajesh Kumar Gazara ◽  
Sandhya Verma ◽  
Madan Kumar ◽  
Praveen Kumar Verma ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sodium Bicarbonate ◽  
Genome Sequence ◽  
Sole Carbon Source

The Serratia sp. strain ISTD04 has been identified as a carbon dioxide (CO 2 )-sequestering bacterium isolated from marble mining rocks in the Umra area, Rajasthan, India. This strain grows chemolithotrophically on media that contain sodium bicarbonate (NaHCO 3 ) as the sole carbon source. Here, we report the genome sequence of 5.07 Mb Serratia sp. ISTD04.

Production of Polyhydroxyalkanoates Copolymers by Recombinant Pseudomonas in Plasmid- and Antibiotic-Free Cultures

2018 ◽  
Vol 28 (5) ◽  
pp. 225-235
Author(s):  
Edmar Ramos Oliveira-Filho ◽  
Linda P. Guamán ◽  
Thatiane Teixeira Mendonça ◽  
Paul F. Long ◽  
Marilda Keico Taciro ◽  
...  
Keyword(s):  
Carbon Source ◽  
Aeromonas Hydrophila ◽  
Sole Carbon Source ◽  
Ralstonia Eutropha ◽  
Pha Synthase ◽  
Medium Chain Length ◽  
Marker Free ◽  
Pha Copolymers ◽  
First Time ◽  
Induced Mutant

Three different polyhydroxyalkanoate (PHA) synthase genes (<i>Ralstonia eutropha</i> H16, <i>Aeromonas</i> sp. TSM81 or <i>Aeromonas hydrophila</i> ATCC7966 <i>phaC</i>) were introduced into the chromosome of two <i>Pseudomonas</i> strains: a native medium-chain-length 3-polyhydroxyalkanoate (PHA<sub>MCL</sub>) producer (<i>Pseudomonas</i> sp. LFM046) and a UV-induced mutant strain unable to produce PHA (<i>Pseudomonas</i> sp. LFM461). We reported for the first time the insertion of a chromosomal copy of <i>phaC</i> using the transposon system mini-Tn<i>7</i>. Stable antibiotic marker-free and plasmid-free recombinants were obtained. Subsequently, P(3HB-<i>co</i>-3HA<sub>MCL</sub>) was produced by these recombinants using glucose as the sole carbon source, without the need for co-substrates and under antibiotic-free conditions. A recombinant harboring <i>A. hydrophila phaC</i> produced a terpolyester composed of 84.2 mol% of 3-hydroxybutyrate, 6.3 mol% of 3-hydroxyhexanoate, and 9.5 mol% of 3-hydroxydecanoate from only glucose. Hence, we were successful in increasing the industrial potential of <i>Pseudomonas</i> sp. LFM461 strain by producing PHA copolymers containing 3HB and 3HA<sub>MCL</sub> using an unrelated carbon source, for the first time in a plasmid- and antibiotic-free bioprocess.

Carbon dioxide as a green carbon source for the synthesis of carbon cages encapsulating porous silicon as high performance lithium-ion battery anodes

Nanoscale ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 5626-5633 ◽  
Author(s):  
Yaguang Zhang ◽  
Ning Du ◽  
Yifan Chen ◽  
Yangfan Lin ◽  
Jinwei Jiang ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Porous Silicon ◽  
Carbon Source ◽  
Lithium Ion Battery ◽  
High Performance ◽  
Lithium Ion ◽  
Porous Si ◽  
First Time

CO2, for the first time, was used as a green carbon source for synthesizing carbon cages encapsulating porous Si as high performance lithium-ion battery anodes.

BACTERIAL METABOLISM OFD-ASPARTATE INVOLVING RACEMIZATION AND DECARBOXYLATION

1966 ◽  
Vol 12 (4) ◽  
pp. 745-751 ◽  
Author(s):  
A. J. Markovetz ◽  
W. J. Cook ◽  
A. D. Larson
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Bacterial Metabolism ◽  
Alanine Racemase ◽  
Whole Cells

A pseudomonad was isolated from soil with D-aspartate serving as sole carbon source. Whole cells and cellular extracts produced carbon dioxide and DL-alanine from D- and L-aspartate. The addition of oxamycin to cellular extracts inhibited the alanine racemase found to be present and it was ascertained that L-alanine was formed from both D- and L-aspartate, indicating that D-aspartate had been racemized to the L-isomer with subsequent decarboxylation to L-alanine.

A Flavobacterium sp. that degrades diazinon and parathion

1973 ◽  
Vol 19 (7) ◽  
pp. 873-875 ◽  
Author(s):  
N. Sethunathan ◽  
T. Yoshida
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Mineral Medium ◽  
Paddy Water

A Flavobacterium sp., isolated from paddy water by enrichment culture technique, decomposed diazinon in a mineral medium as sole carbon source. The bacterium readily hydrolyzed diazinon to 2-isopropyl-6-methyl-4-hydroxy-pyrimidine which was then converted to carbon dioxide. The bacterium also converted parathion to p-nitrophenol. The enzyme involved in the hydrolysis was constitutive.

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp

scholarly journals Assessment of Voltage Influence in Carbon Dioxide Fixation Process by a Photo-Bioelectrochemical System under Photoheterotrophy

2021 ◽  
Vol 9 (3) ◽  
pp. 474
Author(s):  
Sara Díaz-Rullo Edreira ◽  
Silvia Barba ◽  
Ioanna A. Vasiliadou ◽  
Raúl Molina ◽  
Juan Antonio Melero ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Future Development ◽  
Metabolic Pathways ◽  
Co2 Fixation ◽  
Oxygen Demand ◽  
Carbon Uptake ◽  
Bioelectrochemical System ◽  
Electron Sink ◽  
Soluble Carbon

Bioelectrochemical systems are a promising technology capable of reducing CO2 emissions, a renewable carbon source, using electroactive microorganisms for this purpose. Purple Phototrophic Bacteria (PPB) use their versatile metabolism to uptake external electrons from an electrode to fix CO2. In this work, the effect of the voltage (from −0.2 to −0.8 V vs. Ag/AgCl) on the metabolic CO2 fixation of a mixed culture of PPB under photoheterotrophic conditions during the oxidation of a biodegradable carbon source is demonstrated. The minimum voltage to fix CO2 was between −0.2 and −0.4 V. The Calvin–Benson–Bassham (CBB) cycle is the main electron sink at these voltages. However, lower voltages caused the decrease in the current intensity, reaching a minimum at −0.8 V (−4.75 mA). There was also a significant relationship between the soluble carbon uptake in terms of chemical oxygen demand and the electron consumption for the experiments performed at −0.6 and −0.8 V. These results indicate that the CBB cycle is not the only electron sink and some photoheterotrophic metabolic pathways are also being affected under electrochemical conditions. This behavior has not been tested before in photoheterotrophic conditions and paves the way for the future development of photobioelectrochemical systems under heterotrophic conditions.

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