ON THE MORPHOLOGY AND DEVELOPMENT OF INSECT CYTOPLASMIC-POLYHEDROSIS VIRUS PARTICLES

1965 ◽  
Vol 11 (3) ◽  
pp. 497-501 ◽  
Author(s):  
F. T. Bird
Keyword(s):  
Virus Particle ◽  
Inner Core ◽  
Cytoplasmic Polyhedrosis Virus ◽  
Virus Particles ◽  
Early Stages ◽  
Polyhedrosis Virus

The cytoplasmic-polyhedrosis virus particle is an icosahedron about 68 mμ in diameter. It consists of a capsid, made up of two concentric rings, and an inner core. The capsid has 12 projections.Large masses consisting mostly of the capsids of virus particles develop in the cytoplasm of gut cells infected with cytoplasmic-polyhedrosis virus. Cores of the virus particles are found on the outer surfaces of these masses or in material adjacent to them. The complete virus particle is assembled just before it is enveloped by protein and incorporated into a developing polyhedron.Granules, tentatively identified as lysosomes, increase in number and size during the early stages of infection. Other granules and bodies observed in the gut cells are described.

A CYTOPLASMIC POLYHEDROSIS OF HYALOPHORA CECROPIA (LINNAEUS)

1965 ◽  
Vol 11 (4) ◽  
pp. 703-707 ◽  
Author(s):  
W. A. Smirnoff
Keyword(s):  
Electron Microscope ◽  
Viral Infection ◽  
Cytoplasmic Polyhedrosis Virus ◽  
Virus Particles ◽  
Protein Mass ◽  
Viral Particles ◽  
Polyhedrosis Virus ◽  
Hyalophora Cecropia

Hyalophora cecropia (Linnaeus) is susceptible to infection by a cytoplasmic polyhedrosis virus. Electron microscope studies showed spherical viral particles known as "cores" which remain in groups of 12 to 17 subunits. The virus particles were embedded in a protein mass located in craters of the polyhedra. Larvae of the first, second, and third instars were more susceptible to viral infection than later instars. However, up to 50% of the larvae of later instars sometimes survived. The larvae of subsequent instars and the pupa were very resistant and periodic injections of strong dosages of virus material produced no ill effect. In general, larvae infected by this cytoplasmic virus shrank to less than half normal size before death.

THE DEVELOPMENT OF A CYTOPLASMIC POLYHEDROSIS VIRUS IN THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (CLEMENS)

1966 ◽  
Vol 12 (2) ◽  
pp. 337-339 ◽  
Author(s):  
F. T. Bird
Keyword(s):  
Choristoneura Fumiferana ◽  
Spruce Budworm ◽  
Similar Type ◽  
Dense Material ◽  
Envelop Virus ◽  
Cytoplasmic Polyhedrosis Virus ◽  
Virus Particles ◽  
Polyhedral Shape ◽  
Polyhedrosis Virus

Virus particles and polyhedra develop in patches of viroplasm in the cytoplasm of mid-gut cells of larvae of the spruce bud worm, Choristoneura fumiferana (Clemens). Normal components of the cell disintegrate as the disease spreads, and either they completely disappear or mitochondria, ribosomes, and other components form rings in the outer regions of the cell.Polyhedra develop from dense material which appears to attract and envelop virus particles. Only complete particles with large prominent cores are incorporated into growing polyhedra. As the polyhedra mature, they assume a smooth outline and polyhedral shape and either cease to attract virus particles, or their size is limited by the amount of polyhedron protein and numbers of complete virus particles produced in the cell. Although most of the virus particles not incorporated into polyhedra appear incomplete, the large masses of incomplete particles formed in a similar type of disease of another insect were not found in bud worm larvae.

Spontaneous Production of Type C Virus Particles in a Culture Derived from Rat Embryo Cells

1972 ◽  
Vol 30 ◽  
pp. 288-289
Author(s):  
Elizabeth S. Priori ◽  
T. Shigematsu ◽  
B. Myers ◽  
L. Dmochowski
Keyword(s):  
Virus Particle ◽  
Mouse Embryo ◽  
Calf Serum ◽  
Embryo Cell ◽  
Virus Particles ◽  
Extracellular Virus ◽  
Mouse Embryo Cell ◽  
Mature Virus Particle ◽  
Embryo Cells ◽  
Type C

Spontaneous release of type C virus particles in long-term cultures of mouse embryo cells as well as induction of similar particles in mouse embryo cell cultures with IUDR or BUDR have been reported. The presence of type C virus particles in cultures of normal rat embryos has not been reported.NB-1, a culture derived from embryos of a New Zealand Black (NB) rat (rats obtained from Mr. Samuel M. Poiley, N.C.I., Bethesda, Md.) and grown in McCoy's 5A medium supplemented with 20% fetal calf serum was passaged weekly. Extracellular virus particles similar to murine leukemia particles appeared in the 22nd subculture. General appearance of cells in passage 23 is shown in Fig. 1. Two budding figures and one immature type C virus particle may be seen in Fig. 2. The virus particles and budding were present in all further passages examined (currently passage 39). Various stages of budding are shown in Figs. 3a,b,c,d. Appearance of a mature virus particle is shown in Fig. 4.

Sign in / Sign up

Export Citation Format

Share Document