THE MICROBIAL METABOLISM OF CINNAMIC ACID

1964 ◽  
Vol 10 (2) ◽  
pp. 175-185 ◽  
Author(s):  
E. R. Blakley ◽  
F. J. Simpson
Keyword(s):  
Carbon Source ◽  
Cinnamic Acid ◽  
Sole Carbon Source ◽  
Culture Medium ◽  
Microbial Metabolism ◽  
Phenylpropionic Acid ◽  
Sequential Induction

A strain of Pseudomanas isolated from soil with cinnamic acid as a sole carbon source was found to be simultaneously adapted to the utilization of cinnamic acid and phenylpropionic acid. During growth on either of these compounds, o-hydroxyphenylpropionic acid and 2,3-dihydroxyphenylpropionic acid were produced in the culture medium. The organism, when grown on either cinnamic acid or phenylpropionic acid, was adapted to the utilization of m-hydroxyphenylpropionic acid and 2,3-dihydroxyphenylpropionic acid, but not to the utilization of o-hydroxyphenylpropionic acid. According to the principle of sequential induction introduced by Stanier, the initial steps in the metabolism of cinnamic acid appear to involve the intermediates phenylpropionic acid, m-hydroxyphenylpropionic acid, and 2,3-dihydroxyphenylpropionic acid.

scholarly journals The origin of urinary aromatic compounds excreted by ruminants 2. The metabolism of phenolic cinnamic acids to benzoic acid

1982 ◽  
Vol 47 (1) ◽  
pp. 155-164 ◽  
Author(s):  
A. K. Martin
Keyword(s):  
Benzoic Acid ◽  
Cinnamic Acid ◽  
Phenylacetic Acid ◽  
Microbial Metabolism ◽  
The Body ◽  
Hydroxy Acids ◽  
Cinnamic Acids ◽  
Phenylpropionic Acid ◽  
Phenolic Derivatives ◽  
Derivatives Of

1. The extent to which phenolic derivatives of benzoic acid (seven); of phenylacetic acid (one); of 3-phenylpropionic acid (one) and of cinnamic acid (six) served as precursors of the urinary benzoic acid excreted by sheep was determined after administration as continuous drips via rumen or abomasal cannulas.2. Phenolic derivatives of benzoic or of phenylacetic acid were not dehydroxylated to yield aromatic acids following administration via either route.3. Rumen infusion of phenolic derivatives of both 3-phenylpropionic and cinnamic acids gave enhanced rumen concentrations of 3-phenylpropionic acid with negligible amounts of benzoic acid. Between 63 and 106% of the 2-, 3- or 4-hydroxy acids, of the 3,4-dihydroxy acids or of the 3-methoxy, 4-hydroxy acids infused were excreted in the urine as benzoic acid and a variable proportion, characteristic of the individual animal, of up to 20% of the dose as cinnamic acid.4. Abomasal infusion of monohydroxy 3-phenylpropionic and cinnamic acids did not yield urinary benzoic acid increments. However, between 11 and 34% of abomasally-infused disubstituted phenolic cinnamic acids infused were excreted in the urine as benzoic acid due, it is postulated, to entero-hepatic circulation and microbial metabolism of the infused acids in the large intestine.5. It is concluded that rumen microbial metabolism of dietary phenolic cinnamic acids to 3-phenylpropionic acid followed by its absorption and oxidation in the body tissues is responsible for the greater part of the benzoic and cinnamic acids found in ruminant urine.

Isolation of One Strain for Biodegradation of Diesel Fuel from Sea Water

2013 ◽  
Vol 726-731 ◽  
pp. 2424-2427
Author(s):  
Pei Pei Han ◽  
Ping Zhe Jiang ◽  
Xiang He Liu ◽  
Hong Yi Huang ◽  
Wen Qian Duan ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Carbon Source ◽  
Phylogenetic Tree ◽  
Diesel Fuel ◽  
Sole Carbon Source ◽  
Culture Medium ◽  
Sea Water ◽  
Rhodococcus Erythropolis ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

Four diesel fuel degrading microorganisms were isolated from soil and sea water from Crude Oil Terminal at Tianjin Port using diesel fuel as sole carbon source, and they all could degrade diesel fuel. The initial diesel fuel concentration in the culture medium was optimized and was 1.5 g/L. The ability of the four strains to degrade diesel was compared by spectrophotometer with the initial diesel concentration at 1.5 g/L. The results showed that the strain C1 had the highest degrading activity, which could degrade 32.59% of diesel in 7 days. The strain C1 was further identified by 16S rDNA sequence analysis as Rhodococcus erythropolis, and the phylogenetic tree of the strain C1 was constructed.

scholarly journals Production of laccases, cellulases and xylanases of Pleurotus ostreatus grown in liquid-state fermentation

2017 ◽  
Vol 2 (2) ◽  
pp. 169-176
Author(s):  
Edna María Hernández-Domínguez ◽  
Carmen Sánchez ◽  
Gerardo Díaz-Godínez
Keyword(s):  
Carbon Source ◽  
Kinetic Parameters ◽  
Liquid State ◽  
Pleurotus Ostreatus ◽  
Carboxymethyl Cellulose ◽  
Sole Carbon Source ◽  
Culture Medium ◽  
Carbon Sources ◽  
The Third

In this study, activities of laccases, xylanases and cellulases produced by Pleurotus ostreatus in liquid-state fermentation were evaluated. Three fermentations were done by triplicate where the carbon source was changed, one was made with glucose, in another was used carboxymethylcellulose and xylan and in the third the three carbon sources were added, in all cases, copper was added as inducer of laccases. The kinetic parameters of growth of the fungus were obtained. It was observed that this fungus produced the three enzymes evaluated; laccases showed the highest values (34,240 U/L) in the culture medium with glucose as sole carbon source. Cellulases showed their highest activity in the culture medium with xylan and carboxymethylcellulose (12,858 U/L) and xylanases in medium with glucose, carboxymethyl cellulose and xylan (27,153 U/L). Up to 4 isoform of laccases, 2 of xylanase and 2 of cellulases were observed by zymography.

Extracellular polysaccharide of Aspergillus flavus

1981 ◽  
Vol 46 (10) ◽  
pp. 2435-2440 ◽  
Author(s):  
Alžbeta Kardošová ◽  
Jozef Rosík
Keyword(s):  
Carbon Source ◽  
Aspergillus Flavus ◽  
Sole Carbon Source ◽  
Culture Medium ◽  
Main Chain ◽  
Extracellular Polysaccharide ◽  
Water Soluble ◽  
Molar Weight ◽  
Soluble Polysaccharide ◽  
Hydrolysis Of

A crude extracellular polysaccharide was isolated by precipitation with ethanol from the culture medium of Aspergillus flavus, where the sole carbon source was D-galactose; purification afforded a homogeneous water-soluble polysaccharide in 0.05% yield on the weight of the employed carbon source. This polysaccharide had the relative molar weight 55 000 and [α]D22 -4.2° (c 0.5, H2O); upon total hydrolysis it afforded D-mannose and D-galactose in a 1 : 0.44 ratio. The products of hydrolysis of the methylated polysaccharide and also the course of partial acid and enzymic hydrolyses of the polysaccharide showed that the main chain was formed by (1 → 2) β-linked D-mannose units, of which each second, on average, was substituted by monomeric D-galactose units at C(6).

scholarly journals Cinnamic Acid, an Autoinducer of Its Own Biosynthesis, Is Processed via Hca Enzymes in Photorhabdus luminescens

2008 ◽  
Vol 74 (6) ◽  
pp. 1717-1725 ◽  
Author(s):  
Sabina Chalabaev ◽  
Evelyne Turlin ◽  
Sylvie Bay ◽  
Christelle Ganneau ◽  
Emma Brito-Fravallo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Source ◽  
Cinnamic Acid ◽  
Aromatic Compounds ◽  
Sole Carbon Source ◽  
Biosynthetic Pathway ◽  
Phenylalanine Ammonia Lyase ◽  
Carbon Sources ◽  
Degradation Pathway ◽  
Photorhabdus Luminescens

ABSTRACT Photorhabdus luminescens, an entomopathogenic bacterium and nematode symbiont, has homologues of the Hca and Mhp enzymes. In Escherichia coli, these enzymes catalyze the degradation of the aromatic compounds 3-phenylpropionate (3PP) and cinnamic acid (CA) and allow the use of 3PP as sole carbon source. P. luminescens is not able to use 3PP and CA as sole carbon sources but can degrade them. Hca dioxygenase is involved in this degradation pathway. P. luminescens synthesizes CA from phenylalanine via a phenylalanine ammonia-lyase (PAL) and degrades it via the not-yet-characterized biosynthetic pathway of 3,5-dihydroxy-4-isopropylstilbene (ST) antibiotic. CA induces its own synthesis by enhancing the expression of the stlA gene that codes for PAL. P. luminescens bacteria release endogenous CA into the medium at the end of exponential growth and then consume it. Hca dioxygenase is involved in the consumption of endogenous CA but is not required for ST production. This suggests that CA is consumed via at least two separate pathways in P. luminescens: the biosynthesis of ST and a pathway involving the Hca and Mhp enzymes.

scholarly journals Phytotoxin produced by Bipolaris euphorbiae in-vitro is effective against the weed Euphorbia heterophylla

2002 ◽  
Vol 45 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Aneli M. Barbosa ◽  
Cristina G. M. Souza ◽  
Robert F. H. Dekker ◽  
Rafael C. Fonseca ◽  
Dalva T. Ferreira
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Sole Carbon Source ◽  
Host Plants ◽  
Culture Medium ◽  
Virulent Strain ◽  
Fungal Strain ◽  
Malt Extract ◽  
Mato Grosso

Four virulent strain isolates of the fungus, Bipolaris euphorbiae (previously identified as a Helminthosporium sp.), isolated from host plants in four states within Brazil were screened for the production of phytotoxins that promoted wilting and defoliation of the Brazilian weed, Euphorbia heterophylla, commonly found growing among soyabean crops. Only one isolate, B. euphorbiae Strain I (EUPH petropar from Mato Grosso state), produced phytotoxin in-vitro when grown in stationary culture for 7 d at 28 ° C on minimum salts medium supplemented with 1.5 % glucose as the sole carbon source. Phytotoxin was also produced when the fungal strain was grown on fructose, galactose, mannose, xylose and sucrose. The addition of nitrogen source (yeast extract, peptone or malt extract) to the culture medium did not influence phytotoxin production. The phytotoxin produced by Strain I was most active at pH 6.0, stable between pH 3-9, and was highly thermostable, remaining fully active when heated at 90 ° C for 1 h.

The Cultivation of Methylobactreium rhodesianum H13 for Dichloromethane Degradation

2012 ◽  
Vol 482-484 ◽  
pp. 1390-1393
Author(s):  
Run Ye Zhu ◽  
Hong Xia Liu ◽  
Dong Zhi Chen ◽  
Jian Meng Chen ◽  
Zhe Ning Sun
Keyword(s):  
Carbon Source ◽  
Yeast Extract ◽  
Sole Carbon Source ◽  
Large Scale ◽  
Culture Medium ◽  
Sodium Pyruvate ◽  
Toxic Pollutant ◽  
Degradation Activity ◽  
Comparison And Analysis ◽  
Large Scale Cultivation

Dichloromethane (DCM) is a toxic pollutant with prolonged persistence in air and water. The work focused on the cultivation of Methylobacterium rhodesiaum H13 which could utilize DCM as the sole carbon source. By comparison and analysis, R2A was chosen as the suitable culture medium for large-scale cultivation. Furthermore, the optimized composition of medium was established on the basis of biomass obtained and the DCM degradation activity of M. rhodesiaum H13, which contained yeast extract 1.5 g/L, peptone 1.5 g/L, glucose 1.5 g/L, sodium pyruvate 0.9 g/L, K2HPO4 0.9 g/L and MgSO4•H2O 0.15 g/L.

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp
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