AN ELECTRON MICROSCOPIC COMPARISON OF BOVINE INFECTIOUS PUSTULAR VULVOVAGINITIS VIRUS AND INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS IN TISSUE CULTURE

1962 ◽  
Vol 8 (3) ◽  
pp. 373-378 ◽  
Author(s):  
I. Grinyer ◽  
M. Savan ◽  
M. J. Studdert
Keyword(s):  
Tissue Culture ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Phase Contrast ◽  
Kidney Cells ◽  
Infectious Bovine Rhinotracheitis ◽  
Electron Microscopic ◽  
Foetal Kidney ◽  
Electron Microscopes ◽  
Infectious Bovine Rhinotracheitis Virus

An electron microscopic study of IPV and IBR viruses isolated from cattle in Canada is presented. A technique is described with which it is possible to observe the cytopathic changes occurring in the same area of a monolayer, of bovine foetal kidney cells, infected with IPV or IBR virus, with both the phase contrast and electron microscopes. The morphology of these viruses was found to be identical and no difference was observed in their intracellular development.

Histochemical, phase contrast microscopic and electron microscopic study on the parietal cells

1966 ◽  
Vol 1 (2) ◽  
pp. 37-37
Author(s):  
Y. Okuda ◽  
T. Miyake ◽  
S. Ohkawa ◽  
Y. Iwasaki ◽  
M. Ohbayashi ◽  
...  
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Phase Contrast ◽  
Parietal Cells ◽  
Electron Microscopic

An Electron Microscopic Study of Kidney Cells in the Lizard Treated with Mercuric Chloride

1982 ◽  
Vol 40 ◽  
pp. 60-61
Author(s):  
E. C. V. Ooi ◽  
E. C. Chew
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Mercuric Chloride ◽  
Kidney Cells ◽  
Electron Microscopic ◽  
Cellular Effects ◽  
Tissue Alterations

The nephrotoxicity of mercury is well established, especially in mammals and some fishes. However, no information on tissue alterations caused by mercurial compounds in reptiles is available. The present work was to study by using electron microscopy the cellular effects of mercury on kidney cells in the lizard, Gekko gekko. Twelve lizards were given single intraperitoneal injections of 12 mg/kg of mercuric chloride in saline. They were sacrificed after various intervals from 2 to 13 days. Four control animals received only saline injections.

Electron Microscopic Study of the Morphogenesis of Echovirus 23

1973 ◽  
Vol 31 ◽  
pp. 590-591
Author(s):  
C. M. Trant ◽  
R. M. Jamison
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Post Infection ◽  
Glass Surface ◽  
Kidney Cells ◽  
Electron Microscopic ◽  
Monolayer Cultures ◽  
Infected Cells ◽  
Infectious Cycle

The cytopathology which accompanies the replication of Echovirus 23 in monkey kidney cells has been studied. Cells grown in monolayer cultures in glass bottles were infected with Echovirus 23 and incubated at 37°C. At varying intervals, cells were harvested for electron microscopy. Multiple embeddings of specimens at each interval post-infection were performed to confirm and extend the observations. It was noted that as the interval postinfection lengthened, individual infected cells rounded and detached from the surface of the monolayer. As the infectious cycle continued, virtually all cells in the bottle became detached. Accordingly, each infected bottle provided two specimens for electron microscopy; a) those free in the supernatent. fluid and b) those remaining on.the glass surface.

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