Histochemical, phase contrast microscopic and electron microscopic study on the parietal cells

1966 ◽  
Vol 1 (2) ◽  
pp. 37-37
Author(s):  
Y. Okuda ◽  
T. Miyake ◽  
S. Ohkawa ◽  
Y. Iwasaki ◽  
M. Ohbayashi ◽  
...  
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Phase Contrast ◽  
Parietal Cells ◽  
Electron Microscopic

AN ELECTRON MICROSCOPIC COMPARISON OF BOVINE INFECTIOUS PUSTULAR VULVOVAGINITIS VIRUS AND INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS IN TISSUE CULTURE

1962 ◽  
Vol 8 (3) ◽  
pp. 373-378 ◽  
Author(s):  
I. Grinyer ◽  
M. Savan ◽  
M. J. Studdert
Keyword(s):  
Tissue Culture ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Phase Contrast ◽  
Kidney Cells ◽  
Infectious Bovine Rhinotracheitis ◽  
Electron Microscopic ◽  
Foetal Kidney ◽  
Electron Microscopes ◽  
Infectious Bovine Rhinotracheitis Virus

An electron microscopic study of IPV and IBR viruses isolated from cattle in Canada is presented. A technique is described with which it is possible to observe the cytopathic changes occurring in the same area of a monolayer, of bovine foetal kidney cells, infected with IPV or IBR virus, with both the phase contrast and electron microscopes. The morphology of these viruses was found to be identical and no difference was observed in their intracellular development.

scholarly journals An Electron Microscopic Study of the Changes in Platelets during Viscous Metamorphosis

Blood ◽  
1962 ◽  
Vol 20 (5) ◽  
pp. 566-580 ◽  
Author(s):  
P. A. CASTALDI ◽  
B. G. FIRKIN ◽  
P. M. BLACKWELL ◽  
K. I. CLIFFORD
Keyword(s):  
Electron Microscope ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Light Microscope ◽  
Phase Contrast ◽  
Intact Cell ◽  
Complete Loss ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Sectioned Material

Abstract Viscous metamorphosis of platelets has been studied with the light microscope, and ultra-thin sections have been prepared at progressive stages for examination in the electron microscope. The phase contrast light microscope reveals rapid aggregation and distortion of platelets and gives the impression of their fusion into structureless aggregates during viscous metamorphosis. Sectioned material collected during viscous metamorphosis of platelets and examined in the electron microscope reveals a remarkable degree of retention of structure in a majority of the platelets. All become deficient in granules and devoid of vesicular spaces, but most retain intact cell membranes, and the structureless masses seen with the light microscope are found to consist of densely aggregated platelets. Fusion and complete loss of identity occurs in the minority. The retracted clot was found to contain densely aggregated, distorted and elongated platelets, empty of granules and intimately related to fibrin particles.

The Growth of Herpesvirus mulatto in Human Fibroblast

1974 ◽  
Vol 32 ◽  
pp. 244-245
Author(s):  
Glennelle Washington ◽  
Philip P. McGrath ◽  
Peter R. Graze ◽  
Ivor Royston
Keyword(s):  
Rhesus Monkey ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Peripheral Blood ◽  
Human Fibroblast ◽  
Rhesus Monkeys ◽  
Human Fibroblasts ◽  
Electron Microscopic ◽  
Specific Antisera ◽  
Peripheral Blood Leucocytes

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

1971 ◽  
Vol 29 ◽  
pp. 338-339
Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall
Keyword(s):  
Cell Wall ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Genetic Heterogeneity ◽  
Morphological Characteristics ◽  
Electron Microscopic ◽  
Dna Reassociation ◽  
Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

A New Technique for the Light and Electron Microscopic Study of Individual Cerebro-Spinal Fluid Cells in a Mona Plane Layer

1976 ◽  
Vol 34 ◽  
pp. 362-363
Author(s):  
L.A. Dell
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Plane Layer ◽  
Ultrastructural Level ◽  
Spinal Fluid ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
Easy Method ◽  
Cell Pellet ◽  
A New Technique

A new method has been developed which readily offers the microscopist a possibility for both light and electron microscopic study of selected cells from the cerebrospinal fluid. Previous attempts to examine these cells in the spinal fluid at the ultrastructural level were based on modifications of cell pellet techniques developed for peripheral blood. These earlier methods were limited in application by the number of cells in spinal fluid required to obtain a sufficient size pellet and by the lack of an easy method of cellular identification between the light and electron microscopic level. The newly developed method routinely employs microscope slides coated with Siliclad and tungsten oxide for duplicate cytocentrifuge preparations of diagnostic spinal fluid specimens. Work done by Kushida and Suzuki provided a basis for our use of the metal oxide.

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