Bioremediation of diesel fuel contaminated soil

2001 ◽  
Vol 28 (S1) ◽  
pp. 131-140 ◽  
Author(s):  
R G Zytner ◽  
A Salb ◽  
T R Brook ◽  
M Leunissen ◽  
W H Stiver
Keyword(s):  
Diesel Fuel ◽  
Contaminated Soil ◽  
Degradation Rate ◽  
Laboratory Data ◽  
Cold Climate ◽  
Total Petroleum Hydrocarbons ◽  
Northern Ontario ◽  
Spiked Soil ◽  
Degradation Rates ◽  
Rate Correlation

Field and laboratory studies were used to study the influence of temperature and O2on the bioremediation of diesel-fuel contaminated soil. Field data were obtained from a landfarm located in Northern Ontario, whereas laboratory experiments were conducted using bioreactors containing diesel-spiked soil and contaminated soil from the field site. Laboratory and field degradation rates were quantified based on changes in the total petroleum hydrocarbons concentration and some individual components, as well as by monitoring O2consumption and CO2evolution. A degradation rate correlation was developed from the laboratory data. Based on comparison with the laboratory data, the slow rate observed in the field was likely due to low O2concentrations at the site.Key words: bioremediation, diesel fuel, unsaturated soil, cold climate, degradation rate.

Bioremediation of Soil Contaminated by Crude Oil in Daqing, China

2008 ◽  
Author(s):  
Yiling Zhang ◽  
Dehong Kong ◽  
Sheng Sun ◽  
Zhiguo Luo ◽  
Peng Luo
Keyword(s):  
Moisture Content ◽  
Crude Oil ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Degradation Rate ◽  
Total Petroleum Hydrocarbons ◽  
Optimum Conditions ◽  
Optimum Ratio ◽  
Treatment Unit ◽  
Nutrient Ratio

Bioremediation of soil contaminated by pipeline spills in the Daquin region of China is presented in this paper. The objective of the study was to determine the nutrient ratio required for the bioremediation of soil contaminated by Daqing crude oil. Several experiments were conducted using different proportions of C:N:P:K to choose the optimum ratio of nutrients to be applied for bioremediation, C is supplied from the crude oil in the contaminated soil. The moisture content, porosity, PH and temperature of the contaminated soil were adjusted for optimum conditions. The experimental bioremediation technology was conducted in a treatment unit that was 0.5 m in length, 0.7 m in width, and 1.5 m in height. The results showed that the degradation rate of Total Petroleum Hydrocarbons (TPH) increased with increased concentrations of crude oil in the soil. At the beginning of the bioremediation experiments, the degradation rate of THP is high, but it gradually slowed over the course of the experiment. The degradation rate of TPH averaged 98% over 8 months.

scholarly journals Enhanced Natural Biodegradation of Diesel Fuel Contaminants in Soil by Addition of Whey and Nutrients

2017 ◽  
pp. 1001-1008
Author(s):  
Ana Paola Vilches ◽  
Dan Bylund ◽  
Anders Jonsson
Keyword(s):  
Diesel Fuel ◽  
Contaminated Soil ◽  
Organic Amendments ◽  
Soil Samples ◽  
Contaminated Site ◽  
Special Treatment ◽  
Positive Effects ◽  
The North ◽  
Degradation Rates

The contamination of soils by petroleum hydrocarbons, such as diesel fuel, has since many years been a serious environmental problem. Treatment of contaminated areas is a concern for governments and environmental authorities in several countries and efforts have been done with the purpose to eliminate this problem. Different methods have been tested and today the most common technique involves the excavation and transportation of contaminated soil to special treatment facilities. In earlier studies we have demonstrated the effect of adding organic amendments, such as fermented whey, on the biodegradation of n-alkanes in diesel contaminated soil. Non-fermented sweet whey also proved significantly to enhance the biodegradation of an aromatic substance (phenanthrene) in contaminated soil. The current paper presents the results of an in-situ field test at a former gas station in the north of Sweden. In parallel to the field study, biodegradation profiles were monitored under controlled laboratory conditions by taking soil samples from the contaminated site and spike them with diesel fuel. The experiments were carried out by adding whey and mineral nutrients (NPK) to the test area and to the laboratory samples, and monitor the degradation of hydrocarbons by gas chromatographic analysis of extracted soil samples. Significant effects on the degradation rates were achieved in the laboratory tests. For the in-situ test, however, no such positive effects could be registered.

Isolation of Petroleum Hydrocarbon Degrading Bacteria from the Liaohe Estuary at Cold Climate

2015 ◽  
Vol 1092-1093 ◽  
pp. 878-881
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Diesel Oil ◽  
Cold Climate ◽  
Hydrocarbon Degradation ◽  
Oil Degradation ◽  
Degrading Bacteria ◽  
Cold Tolerant ◽  
Diesel Oil Degradation

Two cold-tolerant petroleum hydrocarbon degrading bacteria strain named CHD1 and CHD2 were isolated from oil-contaminated soil at cold climate. The isolated strains were able use diesel oil as sole carbon. The petroleum hydrocarbon degradation rate was analyzed using UV-spectrometry-based methods. The results showed that the diesel oil degradation rate of CHD1 and CHD2 were 22% and 25%, respectively.

scholarly journals Degradation of chlorpyrifos in contaminated soil by immobilized laccase

2016 ◽  
Vol 81 (10) ◽  
pp. 1215-1224 ◽  
Author(s):  
Xin Wang ◽  
Meng Yao ◽  
Li Liu ◽  
Yu Cao ◽  
Jia Bao
Keyword(s):  
Sodium Alginate ◽  
Contaminated Soil ◽  
Environmental Conditions ◽  
Degradation Rate ◽  
Immobilized Laccase ◽  
Fungal Laccase ◽  
Immobilization Time ◽  
Degradation Rates

In the present study, the embedding-crosslinking method was used to immobilize fungal laccase, and to determine the suitable conditions for immobilization by measuring various activities of the immobilized laccase. In addition, the immobilized laccase was further employed to repair chlorpyrifos-contaminated soil, and then the degradation rates of chlorpyrifos were measured under different conditions. Based on the results, the appropriate conditions for the method of mbedding-crosslinking were found to be as follows: concentration of sodium alginate ? 3 %, concentration of glutaraldehyde ? 1 %, crosslinking time ? 6h, volume of crude laccase ? 60 ml, and immobilization time ? 4 h. After 48 h, the degradation rate of chlorpyrifos in soil could reach at least 70 % by the use of immobilized laccase that was prepared by the method of embedding-crosslinking under different environmental conditions.

Composting Study of Petroleum Contaminated Soil

2013 ◽  
Vol 864-867 ◽  
pp. 67-70
Author(s):  
Xue Ying Song ◽  
Ru Jing Liang ◽  
Yu Shuang Li ◽  
Xin Xin Li ◽  
Xiao Jun Hu
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Organic Amendments ◽  
Dry Weight ◽  
Diesel Oil ◽  
Weight Basis ◽  
Total Petroleum Hydrocarbons ◽  
Major Objective ◽  
Degradation Rates ◽  
Petroleum Contaminated Soil

Composting has been shown to be an effective bioremediation technique for the treatment of hydrocarbon-contaminated soil. In this research, the major objective of this research was to find the appropriate mix ratio of organic amendments for enhancing the degradation of petroleum hydrocarbons during diesel oil contaminated soil composting. The spent mushroom was added as an amendment for supplementing organic matter for composting of contaminated soil. The volumn ratios of contaminated soil to organic amendments were 1:1, 1.5:1 and 2:1. Target contaminant of this research was diesel oil, which was spiked at 16240 mg/kg sample on a dry weight basis. The degradation of diesel oil was significantly enhanced by the addition of these organic amendments relative to straight soil control. Degradation rates of total petroleum hydrocarbons (TPH) were the greatest at the ratio of 1:1 of contaminated soil to organic amendments on the volumn ratio. The abiotic loss of TPH was only about 6.83% of initial TPH.

scholarly journals Performance Ratio and Degradation Rate Analysis of 10-Year Field Exposed Residential Photovoltaic Installations in the UK and Ireland

2020 ◽  
Vol 2 (2) ◽  
pp. 170-183
Author(s):  
Mahmoud Dhimish
Keyword(s):  
Degradation Rate ◽  
Cold Climate ◽  
Power Delivery ◽  
Performance Ratio ◽  
Accurate Analysis ◽  
Climate Conditions ◽  
Pv Systems ◽  
Rate Analysis ◽  
Degradation Rates ◽  
The Uk

As photovoltaic (PV) penetration of the power grid increases, accurate predictions of return on investment require accurate analysis of decreased operational power output over time. The degradation rate in PV module performance must be known in order to predict power delivery. This article presents the degradation rates over 10 years for seven different PV systems located in England, Scotland, and Ireland. The lowest PV degradation rates of −0.4% to −0.6%/year were obtained at the Irish PV sites. Higher PV degradation rates of −0.7% to −0.9%/year were found in England, whereas the highest degradation rate of −1.0%/year was observed in relatively cold areas including Aberdeen and Glasgow, located in Scotland. The main reason that the PV systems affected by cold climate conditions had the highest degradation rates was the frequent hoarfrost and heavy snow affecting these PV systems, which considerably affected the reliability and durability of the PV modules and their performance. Additionally, in this article, we analyse the monthly mean performance ratio (PR) for all examined PV systems. It was found that PV systems located in Ireland and England were more reliable compared to those located in Scotland.

scholarly journals Bioremediation of Artificial Diesel-Contaminated Soil Using Bacterial Consortium Immobilized to Plasma-Pretreated Wood Waste

2019 ◽  
Vol 7 (11) ◽  
pp. 497 ◽  
Author(s):  
Ravit Farber ◽  
Alona Rosenberg ◽  
Shmuel Rozenfeld ◽  
Gabi Banet ◽  
Rivka Cahan
Keyword(s):  
Contaminated Soil ◽  
Degradation Rate ◽  
Treated Wood ◽  
Untreated Wood ◽  
Bacterial Consortium ◽  
Bacterial Attachment ◽  
Wood Waste ◽  
Planktonic Bacteria ◽  
Degradation Rates ◽  
Diesel Biodegradation

Bioaugmentation is a bioremediation option based on increasing the natural in-situ microbial population that possesses the ability to degrade the contaminating pollutant. In this study, a diesel-degrading consortium was obtained from an oil-contaminated soil. The diesel-degrading consortium was grown on wood waste that was plasma-pretreated. This plasma treatment led to an increase of bacterial attachment and diesel degradation rates. On the 7th day the biofilm viability on the plasma-treated wood waste reached 0.53 ± 0.02 OD 540 nm, compared to the non-treated wood waste which was only 0.34 ± 0.02. Biofilm attached to plasma-treated and untreated wood waste which was inoculated into artificially diesel-contaminated soil (0.15% g/g) achieved a degradation rate of 9.3 mg day−1 and 7.8 mg day−1, respectively. While, in the soil that was inoculated with planktonic bacteria, degradation was only 5.7 mg day−1. Exposing the soil sample to high temperature (50 °C) or to different soil acidity did not influence the degradation rate of the biofilm attached to the plasma-treated wood waste. The two most abundant bacterial distributions at the family level were Xanthomonadaceae and Sphingomonadaceae. To our knowledge, this is the first study that showed the advantages of biofilm attached to plasma-pretreated wood waste for diesel biodegradation in soil.

Phytoremediation of Petroleum-Contaminated Soil

2011 ◽  
Vol 356-360 ◽  
pp. 2737-2740
Author(s):  
Chun Rong Li ◽  
Abao Wei ◽  
Tao Chen
Keyword(s):  
Field Experiment ◽  
Contaminated Soil ◽  
Control Area ◽  
Degradation Kinetic ◽  
Degradation Rates ◽  
Petroleum Contaminated Soil

Corn, sunflower and alfalfa were taken as remediation plants. Their phytoremediation and degradation kinetic of petroleum were investigated under field experiment. The results indicated that petroleum degradation rates of corn, sunflower and alfalfa remediation areas reached 42.5%, 46.4% and 44.7% after 150 days of remediation, which were increased by 100.5%, 118.9% and 110.8% compared with that in control area, respectively. Petroleum degradation rates of sunflower remediation areas﹥alfalfa remediation areas’﹥corn remediation areas’, whose half-lifes were 165d, 182d and 193d, respectively, which were decreased by 297d, 279d and 269d compared with that in control area, respectively. The remediation effects of corn, sunflower and alfalfa were obvious.

Interaction with Endogenous Microorganisms, Comamonas testosteroni Enhanced the Degradation of Polycyclic Aromatic Hydrocarbon in Soil

2021 ◽  
Author(s):  
Xueting Sun ◽  
Xin Li ◽  
Yue Cui ◽  
Ziwei Jiang ◽  
Qiao Wang ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Structural Equation ◽  
Functional Role ◽  
Degradation Rate ◽  
Structural Equation Models ◽  
Low Molecular Weight ◽  
Comamonas Testosteroni ◽  
Functional Bacteria ◽  
Polycyclic Aromatic

Abstract This study was to explore the functional role of Comamonas testosteroni (Ct) on soil indigenous microorganisms, and analyze the effect of Ct on PAHs degradation in PAH-contaminated soil. Results showed that inoculation of Ct could degrade naphthalene (Nap), phenanthrene (Phe), and benzo [α] pyrene (BaP) significantly. The degradation rate of Nap, Phe and BaP was 81.18%, 63.38% and 37.98% on day 25, respectively, suggesting that the low molecular weight of Nap and Phe were easier to be degraded by microorganisms than BaP. Network analysis showed that inoculation of Ct significantly increased the bacteria closely related to PAHs. Structural equation models confirmed Steroidobacter as functional bacteria could affect the degradation of Nap and BaP. Inoculated Ct could effectively enhance the synergy among indigenous bacteria to degrade PAHs. This would be helpful to understand the function of inoculated strains in PAH-contaminated soil and identify functional microorganisms of PAHs remediation.

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