Unconventional microarray design reveals the response to obesity is largely tissue specific: analysis of common and divergent responses to diet-induced obesity in insulin-sensitive tissues

2012 ◽  
Vol 37 (2) ◽  
pp. 257-268 ◽  
Author(s):  
Robyn K. Lee ◽  
Dustin S. Hittel ◽  
Vongai Z. Nyamandi ◽  
Li Kang ◽  
Jung Soh ◽  
...  
Keyword(s):  
Microarray Design ◽  
Tissue Specific ◽  
Diet Induced Obesity ◽  
Specific Analysis

scholarly journals Tissue-specific expression of Sprouty1 in mice protects against high-fat diet-induced fat accumulation, bone loss and metabolic dysfunction

2011 ◽  
Vol 108 (6) ◽  
pp. 1025-1033 ◽  
Author(s):  
Sumithra Urs ◽  
Terry Henderson ◽  
Phuong Le ◽  
Clifford J. Rosen ◽  
Lucy Liaw
Keyword(s):  
Adipose Tissue ◽  
Bone Loss ◽  
Bone Mass ◽  
Body Fat ◽  
High Fat Diet ◽  
Conditional Knockout ◽  
Whole Body ◽  
High Fat ◽  
Tissue Specific ◽  
Diet Induced Obesity

We recently characterised Sprouty1 (Spry1), a growth factor signalling inhibitor as a regulator of marrow progenitor cells promoting osteoblast differentiation at the expense of adipocytes. Adipose tissue-specific Spry1 expression in mice resulted in increased bone mass and reduced body fat, while conditional knockout of Spry1 had the opposite effect with decreased bone mass and increased body fat. Because Spry1 suppresses normal fat development, we tested the hypothesis that Spry1 expression prevents high-fat diet-induced obesity, bone loss and associated lipid abnormalities, and demonstrate that Spry1 has a long-term protective effect on mice fed a high-energy diet. We studied diet-induced obesity in mice with fatty acid binding promoter-driven expression or conditional knockout of Spry1 in adipocytes. Phenotyping was performed by whole-body dual-energy X-ray absorptiometry, microCT, histology and blood analysis. In conditional Spry1-null mice, a high-fat diet increased body fat by 40 %, impaired glucose regulation and led to liver steatosis. However, overexpression of Spry1 led to 35 % (P < 0·05) lower body fat, reduced bone loss and normal metabolic function compared with single transgenics. This protective phenotype was associated with decreased circulating insulin (70 %) and leptin (54 %; P < 0·005) compared with controls on a high-fat diet. Additionally, Spry1 expression decreased adipose tissue inflammation by 45 %. We show that conditional Spry1 expression in adipose tissue protects against high-fat diet-induced obesity and associated bone loss.

Mechanisms of glycolytic control during facultative anaerobiosis in a marine mollusc: tissue-specific analysis of glycogen phosphorylase and fructose-2,6-bisphosphate

1988 ◽  
Vol 66 (8) ◽  
pp. 1767-1771 ◽  
Author(s):  
Kenneth B. Storey
Keyword(s):  
Glycogen Phosphorylase ◽  
Retractor Muscle ◽  
Dramatic Reduction ◽  
Metabolic Rate Depression ◽  
Tissue Specific ◽  
Marine Mollusc ◽  
Energy Needs ◽  
Specific Analysis ◽  
Anaerobic Energy ◽  
Glycolytic Control

Changes in the activity of glycogen phosphorylase and the content of fructose-2,6-bisphosphate (F-2, 6-P2) were monitored in tissues of the whelk, Busycotypus canaliculatum, over a 21-h course of environmental anoxia. Tissue-specific responses to anoxia were seen with respect to phosphorylase content: in the radular retractor muscle and foot, the content of phosphorylase a expressed rose rapidly over the initial hours of anoxia (maximal increases were 4.3- and 2.5-fold, respectively) while in the gill, content dropped 2-fold during anoxia. Phosphorylase content was modulated by two mechanisms, changes in the percentage of enzyme in the active a form and changes in the total amount (a + b) of enzyme expressed. Anoxia stimulated a dramatic reduction in F-2,6-P2 content in five tissues. In the ventricle, content fell by 224-fold with a t1/2 of only 35 min. Levels in gill, radular retractor, hepatopancreas, and kidney fell to 2.5–3.5% of control values within the first 8 h of anoxia. F-2,6-P2 content in foot muscle was not altered during anoxia. Changes in glycogen phosphorylase activities and F-2,6-P2 contents help to produce tissue-specific responses of glycolysis to environmental anoxia that acknowledge competing metabolic demands including metabolic rate depression, changes in fuel use, anaerobic energy needs, and carbohydrate use for anabolic purposes.

Tissue specific analysis of bioconversion traits in the bioenergy grass Sorghum bicolor

2013 ◽  
Vol 50 ◽  
pp. 118-130 ◽  
Author(s):  
Joshua P. Vandenbrink ◽  
Ryan E. Hammonds ◽  
Roger N. Hilten ◽  
K.C. Das ◽  
J. Michael Henson ◽  
...  
Keyword(s):  
Sorghum Bicolor ◽  
Tissue Specific ◽  
Specific Analysis

Tissue-Specific Changes in Genes Regulating Glucocorticoid Metabolism in Mice with Diet-Induced Obesity

2011 ◽  
pp. P2-462-P2-462
Author(s):  
Erika Harno ◽  
Alice Yu ◽  
Andrew V Turnbull ◽  
Brendan Leighton ◽  
Anne White
Keyword(s):  
Tissue Specific ◽  
Diet Induced Obesity ◽  
Glucocorticoid Metabolism

scholarly journals Tissue‐Specific Analysis of Pharmacological Pathways

2018 ◽  
Vol 7 (7) ◽  
pp. 453-463 ◽  
Author(s):  
Yun Hao ◽  
Kayla Quinnies ◽  
Ronald Realubit ◽  
Charles Karan ◽  
Nicholas P. Tatonetti
Keyword(s):  
Tissue Specific ◽  
Specific Analysis

Tissue-specific distribution of uncoupling proteins in normal rats and rats with high-fat-diet-induced obesity

2009 ◽  
Vol 37 (7) ◽  
pp. 3177-3182 ◽  
Author(s):  
Chun-Mei Zhang ◽  
Ying Gu ◽  
Da-ni Qing ◽  
Jin-gai Zhu ◽  
Chun Zhu ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Uncoupling Proteins ◽  
High Fat ◽  
Tissue Specific ◽  
Diet Induced Obesity ◽  
Specific Distribution

Adipose-Tissue-Specific Expression of Pig ApoR Protects Mice from Diet-Induced Obesity

2020 ◽  
Vol 68 (7) ◽  
pp. 2256-2262
Author(s):  
Miao Ji ◽  
Ke Xu ◽  
Dawei Zhang ◽  
Tingting Chen ◽  
Liangcai Shen ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Diet Induced Obesity
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