Nitric oxide synthase inhibition delays low-frequency stimulation-induced satellite cell activation in rat fast-twitch muscle

2011 ◽  
Vol 36 (6) ◽  
pp. 996-1000 ◽  
Author(s):  
Karen J.B. Martins ◽  
Ian MacLean ◽  
Gordon K. Murdoch ◽  
Walter T. Dixon ◽  
Charles T. Putman
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Satellite Cell ◽  
Translational Control ◽  
Cell Activation ◽  
Mrna Level ◽  
Low Frequency ◽  
Low Frequency Stimulation ◽  
Frequency Stimulation ◽  
Oxide Synthase

This study examined the effect of nitric oxide synthase (NOS) inhibition via Nω-nitro-l-arginine methyl ester (l-NAME) administration on low-frequency stimulation-induced satellite cell (SC) activation in rat skeletal muscle. l-NAME only delayed stimulation-induced increases in SC activity. Also, stimulation-induced increases in hepatocyte growth factor (HGF) mRNA and protein expression were only abrogated at the mRNA level in l-NAME–treated animals. Therefore, early stimulation-induced SC activation appears to be NOS-dependent, while continued activation may involve NOS-independent HGF translational control mechanisms.

Retinoic acid suppression of endothelial nitric oxide synthase in porcine oocyte

2002 ◽  
Vol 80 (8) ◽  
pp. 777-782 ◽  
Author(s):  
Masa-aki Hattori ◽  
Yukio Kato ◽  
Noboru Fujihara
Keyword(s):  
Nitric Oxide ◽  
Retinoic Acid ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Mrna Level ◽  
Dependent Manner ◽  
Hormonal Stimulation ◽  
Enos Mrna ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

The presence of endothelial nitric oxide synthase (eNOS) has been found in porcine oocytes, but its mRNA and protein levels remain relatively constant during hormonal stimulation. The present study was designed to determine the effect of retinoic acid on eNOS regulation in porcine oocytes during follicle-stimulating hormone (FSH) stimulation. Cumulus–oocyte complexes (COCs), prepared from small antral follicles of immature porcine ovaries, were cultured for 15 h and treated with FSH for an additional 48 h. eNOS mRNA and its protein were analyzed by reverse transcription – polymerase chain reaction and Western blotting, respectively. Retinoic acid had an inhibitory effect on the level of oocyte eNOS mRNA in a dose-dependent manner if COCs were exposed to retinoic acid before FSH stimulation. The inhibition of FSH action was reflected in a decrease in expression of c-fos mRNA. eNOS protein also decreased to approximately 50% of the control after exposure to 10 μM retinoic acid. However, the ability of NO synthesis was abolished in the oocytes prepared from retinoic acid pretreated COCs. These results suggest that retinoic acid has a strong inhibitory action on eNOS mRNA level and NO synthesis in the porcine oocyte.Key words: oocyte, retinoic acid, NO synthesis, eNOS, RT–PCR.

scholarly journals A Comparison of the Dynamics of S100B, S100A1, and S100A6 mRNA Expression in Hippocampal CA1 Area of Rats during Long-Term Potentiation and after Low-Frequency Stimulation

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Pavel D. Lisachev ◽  
Mark B. Shtark ◽  
Olga O. Sokolova ◽  
Vladimir O. Pustylnyak ◽  
Mary Yu. Salakhutdinova ◽  
...  
Keyword(s):  
Mrna Level ◽  
Hippocampal Slices ◽  
Low Frequency ◽  
Fold Increase ◽  
Long Term Potentiation ◽  
Low Frequency Stimulation ◽  
Term Potentiation ◽  
Mrna Content ◽  
Frequency Stimulation ◽  
Ca1 Area

The interest in tissue- and cell-specific S100 proteins physiological roles in the brain remains high. However, necessary experimental data for the assessment of their dynamics in one of the most important brain activities, its plasticity, is not sufficient. We studied the expression of S100B, S100A1, and S100A6 mRNA in the subfield CA1 of rat hippocampal slices after tetanic and low-frequency stimulation by real-time PCR. Within 30 min after tetanization, a 2–4 fold increase of the S100B mRNA level was observed as compared to the control (intact slices) or to low-frequency stimulation. Subsequently, the S100B mRNA content gradually returned to baseline. The amount of S100A1 mRNA gradually increased during first hour and maintained at the achieved level in the course of second hour after tetanization. The level of S100A6 mRNA did not change following tetanization or low-frequency stimulation.

scholarly journals Short-Term Amygdala Low-Frequency Stimulation Does not Influence Hippocampal Interneuron Changes Observed in the Pilocarpine Model of Epilepsy

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 520
Author(s):  
István Mihály ◽  
Tímea Molnár ◽  
Ádám-József Berki ◽  
Réka-Barbara Bod ◽  
Károly Orbán-Kis ◽  
...  
Keyword(s):  
Cell Density ◽  
Basolateral Amygdala ◽  
Low Frequency ◽  
Low Frequency Stimulation ◽  
Male Wistar Rats ◽  
Pilocarpine Model ◽  
Frequency Stimulation ◽  
Stimulation Period ◽  
Oxide Synthase ◽  
Deep Brain

Temporal lobe epilepsy (TLE) is characterized by changes in interneuron numbers in the hippocampus. Deep brain stimulation (DBS) is an emerging tool to treat TLE seizures, although its mechanisms are not fully deciphered. We aimed to depict the effect of amygdala DBS on the density of the most common interneuron types in the CA1 hippocampal subfield in the lithium-pilocarpine model of epilepsy. Status epilepticus was induced in male Wistar rats. Eight weeks later, a stimulation electrode was implanted to the left basolateral amygdala of both pilocarpine-treated (Pilo, n = 14) and age-matched control rats (n = 12). Ten Pilo and 4 control animals received for 10 days 4 daily packages of 50 s 4 Hz regular stimulation trains. At the end of the stimulation period, interneurons were identified by immunolabeling for parvalbumin (PV), neuropeptide Y (NPY), and neuronal nitric oxide synthase (nNOS). Cell density was determined in the CA1 subfield of the hippocampus using confocal microscopy. We found that PV+ cell density was preserved in pilocarpine-treated rats, while the NPY+/nNOS+ cell density decreased significantly. The amygdala DBS did not significantly change the cell density in healthy or in epileptic animals. We conclude that DBS with low frequency applied for 10 days does not influence interneuron cell density changes in the hippocampus of epileptic rats.

Changes in satellite cell content and myosin isoforms in low-frequency-stimulated fast muscle of hypothyroid rat

1999 ◽  
Vol 86 (1) ◽  
pp. 40-51 ◽  
Author(s):  
Charles T. Putman ◽  
Sabine Düsterhöft ◽  
Dirk Pette
Keyword(s):  
Satellite Cell ◽  
Contractile Activity ◽  
Low Frequency ◽  
Thick Filament ◽  
Myosin Isoforms ◽  
Cell Content ◽  
Mhc I ◽  
Low Frequency Stimulation ◽  
Frequency Stimulation ◽  
Mhc Iia

Chronic low-frequency stimulation was used to study the effects of enhanced contractile activity on satellite cell content and myosin isoform expression in extensor digitorum longus muscles from hypothyroid rats. As verified by immunohistochemical staining for desmin, vimentin, and myosin heavy chain (MHC) isoforms and by histological analysis, stimulation induced a transformation of existing fast fibers toward slower fibers without signs of fiber deterioration or regeneration. Immunohistochemically detected increases in MHC I and MHC IIa isoforms, as well as reduced numbers of fibers expressing the faster MHC isoforms, mirrored the rearrangement of the thick-filament composition. These changes, especially the upregulation of MHC IIa, were accompanied by an induction of developmental MHC isoforms in the transforming adult fibers. Satellite cell content rose 2.6-, 3.0-, and 3.7-fold over that of corresponding controls ( P < 0.05 in all cases) in 5-, 10-, and 20-day-stimulated muscles, respectively. Hypothyroidism alone had no effect on satellite cell content but resulted in a significant reduction in fiber size. The relative satellite cell contents increased ( P< 0.05) from 3.8% in euthyroid control muscles to 7.9, 11.5, and 13.8% in the 5-, 10-, and 20-day-stimulated hypothyroid muscles, respectively. In 20-day-stimulated muscles, the relative satellite cell content reached an almost twofold higher level than that of normal slow-twitch soleus muscle. This increase occurred concomitantly with a rise in myonuclear density, most probably because of the fusion of satellite cells with existing fibers.

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