A Decrease in Soleus Muscle Force Generation in Rats After Downhill Running

2001 ◽  
Vol 26 (4) ◽  
pp. 323-335 ◽  
Author(s):  
Antonios Kyparos ◽  
Chrysoula Matziari ◽  
Maria Albani ◽  
Georgios Arsos ◽  
Sofia Sotiriadou ◽  
...  
Keyword(s):  
Soleus Muscle ◽  
Muscle Injury ◽  
Low Frequency ◽  
Eccentric Contraction ◽  
Contractile Properties ◽  
Tetanic Force ◽  
Time To Peak ◽  
Downhill Running ◽  
Male Wistar Rats ◽  
Isometric Twitch

The purpose of the present study was to investigate the immediate and 48-hr post-exercise effects of eccentric contraction-biased exercise on the contractile properties of the soleus muscle in situ. Adult male Wistar rats were categorised into sedentary control rats (n = 10), rats studied immediately (n = 10), and rats studied 48 hours after the exercise (n = 10). The exercise protocol consisted of a 90-min intermittent downhill running (-16°, 16 m/min) on a motor-driven treadmill. The contractile properties of the soleus muscle were recorded following i.p. chloral hydrate anaesthesia. Isometric twitch force (Pt), time-to-peak tension (TPT), half-relaxation time (1/2 RT), and tetanic force at stimulation frequencies of 40, 80, and 100 Hz were recorded. A low-frequency muscle fatigue protocol (stimulation at 4 Hz for 5 min) was applied to test for fatigability. The main findings indicated that Pt generation dropped both immediately and 48 hr after the exercise, while tetanic force was partially restored after 48 hr. Exercise-induced E-C coupling failure and contractile machinery disorganisation due to muscle injury are put forward as the main force reduction causes. Key words: eccentric exercise, muscle injury, SR, E-C coupling, tension recording

Isometric contractile properties and velocity of shortening during avian myogenesis

1982 ◽  
Vol 243 (3) ◽  
pp. C177-C183 ◽  
Author(s):  
P. J. Reiser ◽  
B. T. Stokes ◽  
J. A. Rall
Keyword(s):  
Atpase Activity ◽  
Myosin Atpase ◽  
Contractile Properties ◽  
In Ovo ◽  
Tetanic Force ◽  
Time To Peak ◽  
Myosin Atpase Activity ◽  
Velocity Of Shortening ◽  
Isometric Twitch ◽  
Force Time

Isometric twitch and tetanic contractile properties and velocity of unloaded shortening (V0) of whole avian posterior latissimus dorsi muscle (PLD) were examined between embryonic day 15 and the first 2 wk after hatching. The time to peak twitch force, time to half-relaxation of the twitch response, and time to half-peak tetanic force all change significantly during the final week in ovo but do not change during the first 2 wk ex ovo. Comparisons with previously published reports by others indicate that the twitch half-relaxation time at hatching is approximately the same as that of the adult PLD. The velocity of unloaded shortening increases 2.3-fold during the period studied. It has previously been shown by other that the velocity of shortening is well correlated with a muscle's myosin ATPase activity. Therefore, the observed changes in V0 suggest that the myosin ATPase activity of the avian PLD increases between embryonic day 15 and the first 2 wk posthatching, and this change could account, at least in part, for some of the changes in the isometric properties that were measured.

Soleus muscle force following downhill running in ovariectomized rats treated with estrogen

2006 ◽  
Vol 31 (4) ◽  
pp. 449-459 ◽  
Author(s):  
Sofia Sotiriadou ◽  
Antonios Kyparos ◽  
Maria Albani ◽  
Georgios Arsos ◽  
Mark S.F. Clarke ◽  
...  
Keyword(s):  
Soleus Muscle ◽  
Muscle Injury ◽  
Ovariectomized Rats ◽  
Tetanic Force ◽  
Downhill Running ◽  
Ovx Rats ◽  
17Β Estradiol ◽  
Maximal Tetanic Force ◽  
40 Hz

The ovariectomized (OVX) rat model was used to investigate the effects of estrogen treatment on soleus muscle functionality in situ following muscle injury induced by downhill running. Fifty immature, 24- to 26-d-old, OVX rats were randomly assigned to 5 separate experimental groups: sedentary controls (OVX-Sed), placebo-treated and studied immediately after exercise (OVX-Pb0), placebo-treated and studied 72 h after exercise (OVX-Pb72), estradiol-treated and studied immediately after exercise (OVX-Ed0), and estradiol-treated and studied 72 h after exercise (OVX-Ed72). At the age of 9 weeks, under ketamine and xylazine anesthesia i.p., the rats were subcutaneously implanted with either placebo or 17β-estradiol-impregnated pellets (0.05 mg/pellet, 3 week release). Treatment with 17β-estradiol increased the estradiol plasma levels in OVX animals to those normally seen during the proestrous cycle of normal animals. Three weeks after the implantation the rats were subjected to a 90 min intermittent downhill running protocol. Our results indicate that the exercise protocol used in the study induced injury in the soleus muscle, as it was detected by the significant reduction in unfused (stimulation at 10, 20, and 40 Hz) and maximal (Po) tetanic force, as well as the decreased ability of the soleus muscle to maintain tension (stimulation at 40 Hz for 3 min) in OVX-Pb0 and OVX-Pb72 placebo-treated animals subjected to downhill running (injured muscles) as compared with OVX-Sed control rats (uninjured muscle). Estradiol replacement in OVX rats partially protected the soleus muscle from the injury normally induced by downhill running. As compared with the OVX-Pb0 and OVX-Pb72 placebo-treated groups, the soleus muscles of OVX-Ed0 and OVX-Ed72 estradiol-treated rats were capable of producing significantly greater unfused tetanic force and had an increased ability to maintain tension after fatigue. However, estrogen at the dose administered did not prevent the decrease in maximal tetanic force. We postulate that the protective effect of estrogens on muscle strength may be related to the ability of estrogen hormones to attenuate the E–C coupling failure and (or) the disorganization of the contractile apparatus associated with eccentric exercise through a mechanism or mechanisms yet to be fully understood.

Functional significance of compensatory overloaded rat fast muscle

1982 ◽  
Vol 52 (2) ◽  
pp. 473-478 ◽  
Author(s):  
R. R. Roy ◽  
I. D. Meadows ◽  
K. M. Baldwin ◽  
V. R. Edgerton
Keyword(s):  
Biochemical Properties ◽  
Slow Muscle ◽  
Repetitive Stimulation ◽  
Contractile Properties ◽  
Sectional Area ◽  
Shortening Velocity ◽  
Cross Sectional ◽  
Time To Peak ◽  
Isometric Twitch

Chronic overload of a skeletal muscle by removing its synergists produces hypertrophy and marked changes in its metabolic and biochemical properties. In this study alterations in the contractile properties of the plantaris 12–14 wk after bilateral removal of the soleus and gastrocnemius were investigated. In situ isometric and isotonic contractile properties of overloaded plantaris (OP), normal plantaris (NP), and normal soleus (NS) were tested at 33 +/- 1 degree C. Op were 97% heavier than NP and produced 43 and 46% higher twitch (Pt) and tetanic (Po) tensions. However, NP produced more tension per cross-sectional area than OP (mean 26.2 vs. 21.6 N/cm2; P less than 0.001). Isometric twitch time to peak tension (TPT) and half-relaxation time (1/2RT) were significantly longer in OP (mean 36.4 vs. 32.5 ms and 23.9 vs. 18.4 ms). Mean maximum shortening velocity (Vmax, mm/s per 1,000 sarcomeres) were 34.1 for NP and 18.1 for OP (P less than 0.001). The degree of conversion toward the Vmax of NS was 74% compared with only 19 and 14% for TPT and 1/2RT. OP produced a higher proportion of Po at a given stimulation frequency than NP and showed less fatigue than NP after repetitive stimulation. Chronic overload of the fast plantaris modified to varying degrees the contractile properties studied toward that resembling a slow muscle. Although the maximum tension of OP was markedly enhanced it was not in proportion to the increase in muscle mass.

Average but not continuous speed match between motoneurons and muscle units of rat tibialis anterior

1993 ◽  
Vol 70 (4) ◽  
pp. 1300-1306 ◽  
Author(s):  
R. Bakels ◽  
D. Kernell
Keyword(s):  
Tibialis Anterior ◽  
Time Course ◽  
Muscle Fibers ◽  
Total Duration ◽  
Medial Gastrocnemius ◽  
Tetanic Force ◽  
Time To Peak ◽  
Time Courses ◽  
Isometric Twitch ◽  
Fatigue Sensitivity

1. Properties of single motoneuron/muscle-unit combinations were determined for tibialis anterior (TA) in rats anesthetized with pentobarbital. The TA observations were systematically compared with those obtained earlier by the use of the same techniques from rat medial gastrocnemius (MG). 2. TA motoneurons were investigated with regard to afterhyperpolarization (AHP; total duration 32-74 ms, amplitude 0.39-4.96 mV) and axonal conduction velocity (41-79 m/s). TA muscle-unit measurements included the time course of the isometric twitch (time-to-peak force 10.8-18.0 ms; total duration 42-92 ms), the maximum tetanic force (22-217 mN), and a measure of fatigue sensitivity (fatigue index 5-100%). The range of twitch and AHP durations ("speed range") was markedly smaller in the present TA material than for MG. 3. The mean duration of the TA motoneuronal AHP (49 +/- 8 ms, mean +/- SD) was close to that of its muscle-unit twitch (56 +/- 12 ms). Thus an "average" speed match existed between TA motoneurons and their muscle fibers. 4. For TA there was no correlation between the time courses of AHP and twitch. Thus there was for TA no "continuous" speed match between the motoneurons and their muscle fibers. 5. For TA twitches or AHPs studied separately, there was a significant correlation between different time course measures. Furthermore, compared with TA units having relatively fast twitches, those with slower twitches tended to show 1) a smaller maximum tetanic force and 2) a greater AHP amplitude. Fatigue-resistant units tended to have slower twitches than fatigue-sensitive ones.(ABSTRACT TRUNCATED AT 250 WORDS)

Isometric contractile properties and instantaneous stiffness of amphibian skeletal muscle in the temperature range from 0 to 20 °C

1981 ◽  
Vol 59 (6) ◽  
pp. 548-554 ◽  
Author(s):  
Bernard H. Bressler
Keyword(s):  
Relaxation Time ◽  
Elevated Temperatures ◽  
Bufo Bufo ◽  
Contractile Properties ◽  
Temperature Range ◽  
Time To Peak ◽  
Intracellular Ca ◽  
Cross Bridges ◽  
Isometric Twitch ◽  
Toad Bufo

The isometric contractile properties of frog (Rana pipiens) and toad (Bufo bufo) sartorii have been studied over the temperature range from 0 to 20 °C. The isometric twitch tension was found to vary considerably between these two species and between muscles in the same species. Between 0 and 4 °C ther was very little change in maximum isometric twitch tension. Between 4 and 12 °C several muscles from frog or toad showed a potentiation of twitch tension whereas others showed a decline. Over this temperature range the toad sartorii consistently demonstrated a greater potentiation. By 12 °C a steady decline in twitch tension in both muscles was seen as the temperature approached 20 °C. The maximum isometric tetanic tension recorded between 18 and 20 °C increased fractionally to an average of 1.504 ± 0.029 (n = 4) for frog sartorii and to 1.377 ± 0.008 (n = 5) for toad sartorii. The time to peak twitch tension and the half-relaxation time decreased markedly with an increase in temperature. Moreover, the half-relaxation time was reduced by a greater proportion than the time to peak twitch tension. Measurements of instantaneous stiffness by controlled velocity releases from the plateau of isometric tetani revealed that the large increase in isometric tetanus tension as the muscle was warmed was not accompanied by a corresponding increase in the total number of active cross-bridges. The possibility that a decreased availability of intracellular Ca2+ ions at the contractile sites contributing to the fall of isometric twitch tension at elevated temperatures is discussed. The possibility exists that at elevated temperatures a change in the intrinsic-contractile ability of the muscle occurs which produces an increased tension per cross-bridge.

Coordinate downregulation of CaM kinase II and phospholamban accompanies contractile phenotype transition in the hyperthyroid rabbit soleus

2004 ◽  
Vol 287 (3) ◽  
pp. C622-C632 ◽  
Author(s):  
M. Jiang ◽  
A. Xu ◽  
D.L. Jones ◽  
N. Narayanan
Keyword(s):  
Soleus Muscle ◽  
Cam Kinase Ii ◽  
Cam Kinase ◽  
Sr Protein ◽  
Time To Peak ◽  
Contraction Duration ◽  
Plasmic Reticulum ◽  
Dependent Protein ◽  
Isometric Twitch ◽  
And Function

This study investigated the effects of l-thyroxine-induced hyperthyroidism on Ca2+/calmodulin (CaM)-dependent protein kinase (CaM kinase II)-mediated sarcoplasmic reticulum (SR) protein phosphorylation, SR Ca2+ pump (Ca2+-ATPase) activity, and contraction duration in slow-twitch soleus muscle of the rabbit. Phosphorylation of Ca2+-ATPase and phospholamban (PLN) by endogenous CaM kinase II was found to be significantly lower (30–50%) in soleus of the hyperthyroid compared with euthyroid rabbit. Western blotting analysis revealed higher levels of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) 1 (∼150%) Ca2+ pump isoform, unaltered levels of SERCA2 Ca2+ pump isoform, and lower levels of PLN (∼50%) and δ-, β-, and γ-CaM kinase II (40 ∼ 70%) in soleus of the hyperthyroid rabbit. SR vesicles from hyperthyroid rabbit soleus displayed approximately twofold higher ATP-energized Ca2+ uptake and Ca2+-stimulated ATPase activities compared with that from euthyroid control. The Vmax of Ca2+ uptake (in nmol Ca2+·mg SR protein−1·min−1: euthyroid, 818 ± 73; hyperthyroid, 1,649 ± 90) but not the apparent affinity of the Ca2+-ATPase for Ca2+ (euthyroid, 0.97 ± 0.02 μM, hyperthyroid, 1.09 ± 0.04 μM) differed significantly between the two groups. CaM kinase II-mediated stimulation of Ca2+ uptake by soleus muscle SR was ∼60% lower in the hyperthyroid compared with euthyroid. Isometric twitch force of soleus measured in situ was significantly greater (∼36%), and the time to peak force and relaxation time were significantly lower (∼30–40%), in the hyperthyroid. These results demonstrate that thyroid hormone-induced transition in contractile properties of the rabbit soleus is associated with coordinate downregulation of the expression and function of PLN and CaM kinase II and selective upregulation of the expression and function of SERCA1, but not SERCA2, isoform of the SR Ca2+ pump.

Development of contractile properties in avian embryonic skeletal muscle

1982 ◽  
Vol 242 (1) ◽  
pp. C52-C58 ◽  
Author(s):  
P. J. Reiser ◽  
B. T. Stokes
Keyword(s):  
Muscle Development ◽  
Force Production ◽  
Contractile Properties ◽  
In Ovo ◽  
Tetanic Force ◽  
Time To Peak ◽  
Time Differential ◽  
Fast Twitch Muscle ◽  
Fast Twitch ◽  
Kinetics Of

The development of the twitch and tetanic responses of the embryonic chick posterior latissimus dorsi muscle has been studied during the last week in ovo. Normalized twitch and tetanic forces increased 3- and 12-fold, respectively, during this period. The changes in the kinetics of the twitch and tetanic responses differed during this developmental period. The time to peak twitch force progressively decreased. The decrease in time to half-peak tetanic force and the increase in the time differential of force production of the tetanic response did not continue after day 18. A prolonged tonic contractile component was described for both the twitch and tetanic responses, particularly in muscles from the younger embryos (days 14-18). A large decrease in the time to one-half relaxation of the twitch response also takes place during the final week in ovo. This detailed description of the development of the contractile properties provides a model system of fast-twitch muscle development in which neurogenic and myogenic components of muscular differentiation can be studied from several approaches.

Contractile, biochemical, and histochemical properties of thyrotoxic rat soleus muscle

1980 ◽  
Vol 238 (1) ◽  
pp. C15-C20 ◽  
Author(s):  
R. H. Fitts ◽  
W. W. Winder ◽  
M. H. Brooke ◽  
K. K. Kaiser ◽  
J. O. Holloszy
Keyword(s):  
Relaxation Time ◽  
Sarcoplasmic Reticulum ◽  
Soleus Muscle ◽  
Biochemical Properties ◽  
Contractile Properties ◽  
Contraction Time ◽  
Shortening Velocity ◽  
Actomyosin Atpase ◽  
Atpase Reaction ◽  
Isometric Twitch

The effects of thyrotoxicosis on the contractile properties of soleus muscle were examined in rats given 3 mg of T4 and 1 mg of T3 per kg of diet for 6–8 wk. Thyrotoxicosis induced significant decreases in isometric twitch contraction time (CT), one-half relaxation time, and peak twitch tension. The Ca2+ uptake activity of the sarcoplasmic reticulum (SR) was increased in the thyrotoxic muscles; this adaptation in the SR provides a possible mechanism for the alterations in isometric contractile properties. Thyrotoxicosis induced a large increase in fibers classified as type 2, on the basis of an alkali-stable histochemical reaction for ATPase, in the soleus. Although this reaction is commonly interpreted as indicating that a muscle is fast, maximum shortening velocity (Vmax) and Mg2+ activated actomyosin ATPase activity were unaffected in the thyrotoxic soleus. Our findings provide evidence that CT and Vmax can vary independently and that the histochemical ATPase reaction may not always reflect the biochemical properties that make myosin fast or slow. actomyosin ATPase; histochemical ATPase reaction; isometric contraction time; maximum shortening velocity; one-half relaxation time; sarcoplasmic reticulum; skeletal muscle Submitted on April 13, 1979 Accepted on August 7, 1979

scholarly journals Ultrasound Imaging Evaluation of Textural Features in Athletes with Soleus Pathology—A Novel Case-Control Study

2021 ◽  
Vol 18 (4) ◽  
pp. 1983 ◽  
Author(s):  
Blanca De-la-Cruz-Torres ◽  
Emmanuel Navarro-Flores ◽  
Daniel López-López ◽  
Carlos Romero-Morales
Keyword(s):  
Ultrasound Imaging ◽  
Soleus Muscle ◽  
Muscle Injury ◽  
Intraclass Correlation ◽  
Muscle Thickness ◽  
Tendon Injury ◽  
Muscle Injuries ◽  
Imaging Evaluation ◽  
Rater Reliability ◽  
Group A

Background: the aim of this study was to compare the echotexture of patients with soleus muscle injury and age matched controls. Methods: a sample of 62 athletes was recruited at the private clinic and was divided in two group: a healthy group (n = 31) and a soleus pathology group whose athletes had soleus muscle injury, located in the central tendon (n = 31). The muscle thickness (MTh), echointensity (EI) and echovariation (EV) were analyzed. An intra-rater reliability test (Intraclass Correlation Coefficient-ICC) was performed in order to analyze the reliability of the values of the measurements. Results: Sociodemographic variables did not show statistically significant differences (p > 0.05). Ultrasound imaging measurements who reported statistically significant differences were EI (p = 0.001) and standard deviation (SD) (p = 0.001). MTh and EV variables did not show statistically significant differences (p = 0.381 and p = 0.364, respectively). Moreover, reliability values for the MTh (ICC = 0.911), EI (ICC = 0.982), SD (ICC = 0.955) and EV (ICC = 0.963). Based on these results the intra-rater reliability was considered excellent. Conclusion: Athletes with a central tendon injury of soleus muscle showed a lower EI when they were compared to healthy athletes. The echogenicity showed by the quantitative ultrasound imaging measurement may be a more objective parameter for the diagnosis and follow-up the soleus muscle injuries.

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