Genetic and biochemical variability among Moringa oleifera Lam. accessions collected from different agro-ecological zones

Genome ◽  
2020 ◽  
Vol 63 (3) ◽  
pp. 169-177
Author(s):  
Apurva Panwar ◽  
Jyoti Mathur
Keyword(s):  
Hplc Analysis ◽  
Dna Polymorphism ◽  
Moringa Oleifera ◽  
Biologically Active ◽  
Active Components ◽  
Ecological Zones ◽  
Rapd Pcr ◽  
Biochemical Variability ◽  
Moringa Oleifera Lam ◽  
Agro Ecological Zones

Genomic DNA polymorphism and variation in biologically active components of Moringa oleifera were investigated by two different techniques: RAPD-PCR and HPLC analysis. The concentrations of phenolic compounds (cinnamic, caffeic, ferulic, and coumaric acids) and the content of flavonoids (rutin) were quantified by HPLC analysis. Among 20 RAPD primers, 13 were selected to generate polymorphic amplicons producing an average of 5028 bands, of which 83.7% were found to be polymorphic among 57 accessions of M. oleifera (MO 1 to MO 57) and one outgroup (ACB 58) from Banasthali region, India. In total, 57 accessions were clustered into five major groups within the dendrogram. The results of this analysis were further confirmed by principal coordinate analysis (PCoA). There was also high diversity in the concentration of active compounds in the collected samples as revealed by HPLC analysis. The data revealed that the content of polyphenolic compounds varied between 0.06 (sample KVKB) and 210.5 mg/kg (sample BG). The results suggest that there is a strong correlation between phytochemical variables and DNA polymorphism. The study concludes that the results of the genetic, morphological, and phytochemical diversity could be used to select the best accessions of M. oleifera for agricultural cultivation and breeding.

HPLC analysis, cytotoxicity, and safety study of Moringa oleifera Lam. (wild type) leaf extract

2020 ◽  
Vol 44 (10) ◽  
Author(s):  
Ammara Saleem ◽  
Mohammad Saleem ◽  
Muhammad Furqan Akhtar ◽  
Mirza Muhammad Faran Ashraf Baig ◽  
Azhar Rasul
Keyword(s):  
Hplc Analysis ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Wild Type ◽  
Safety Study ◽  
Moringa Oleifera Lam

scholarly journals Nutrient composition of Moringa oleifera leaves from two agro ecological zones in Ghana

2014 ◽  
Vol 8 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Jasper Asante William ◽  
Latif Nasare Iddrisu ◽  
Tom-Dery Damian ◽  
Ochire-Boadu Kwame ◽  
Bernard Kentil Kwami
Keyword(s):  
Moringa Oleifera ◽  
Nutrient Composition ◽  
Ecological Zones ◽  
Moringa Oleifera Leaves ◽  
Agro Ecological Zones

PRODUCTION OF BLENDED JUICE FROM PUMPKIN, QUINCE, PERSIMMON

2018 ◽  
pp. 59-62
Author(s):  
I. A. Kyazimova ◽  
А. А. Kasumova ◽  
А. А. Nabiev
Keyword(s):  
Phenolic Compounds ◽  
Organic Acids ◽  
New Technology ◽  
Mineral Elements ◽  
Biologically Active ◽  
Active Components ◽  
Aliphatic Acids ◽  
Free Glucose ◽  
Dietary Value ◽  
Quantitative Indicators

Production of plant products, including juices around the world increases continuously. In the fruit and vegetable juices contain a significant amount of monosaccharides (glucose and fructose), organic acids, vitamins, phenolic compounds, mineral substances and other biologically active components that determine the nutritional and dietary value. For the prevention of various diseases associated with impaired metabolic processes, we developed a new technology of preparation of food by blending juice of pumpkin, quince and persimmon. Thus prepared organic blended juice contains a substantial amount of free glucose and fructose, different phenolic compounds, a sufficient amount of organic acids, mineral elements, including iodine and other components that determine its nutritional and biological value. In prepared juices were evaluated the quantitative indicators of β-carotene, vitamin C, glucose and fructose, sucrose, starch, pectin substances. Also in the atomic absorbtion spectrometer Analyst 400 (PerkinElmer, USA) was analyzed content of the organic acids and phenolic compounds. Prepared juices were tested in accordance with 10 point scoring scale. It is established that all juices contain a sufficient amount of the minerals. In pumpkin and quince juices not contain iodine while it presents in sufficient amount in persimmon juice that’s why in the blended juice mineral in addition to mineral elements iodine are contained. In pumpkin and persimmon aliphatic acids are contained in small amount. For this reason during the blending process was used quince juice which is rich in aliphatic acids. The blended juice is light straw color, with delicious flavor, a slight astringent property and a balanced taste.

Monthly variation and impact of pollution on the nutritional value of Moringa oleifera (Lam.) Leaves

2017 ◽  
Vol 6 (10) ◽  
pp. 5535
Author(s):  
Sachin Umesh Dubey ◽  
Madhu Kanta Kapoor
Keyword(s):  
Vitamin C ◽  
Nutritional Value ◽  
Moringa Oleifera ◽  
Reducing Sugars ◽  
Essential Amino Acids ◽  
Monthly Variation ◽  
Vehicular Pollution ◽  
Total Sugars ◽  
Urban Sewage ◽  
Moringa Oleifera Lam

Moringa oleifera Lam., commonly found tree in sub-Himalayan tracts of India, Pakistan, Bangladesh and Afghanistan has a high nutrition value. The leaves, flowers and immature pods of Moringa are used as a vegetable in many countries. The leaves are highly nutritious and medicinal in nature. They are a rich source of iron, calcium, vitamin A, vitamin C, proteins, and essential amino acids. Hence Moringa leaves can be a good source of protein for the vegetarians and the under-nourished population. Present investigation deals with the study of monthly variation in the nutritional value of Moringa leaves from the month of June 2015 to January 2016. Impact of urban sewage pollution and roadside vehicular pollution on the amount of reducing and total sugars, chlorophylls (a, b and total), proteins, vitamin-C and pH of the leaves, was also studied. Results revealed that the highest amounts of reducing sugars, total sugars, chlorophyll b and total chlorophyll during October 2015 whereas proteins, chlorophyll a, vitamin C and pH were highest during January 2016. Leaf samples collected from all the study sites exhibited minimum amounts of reducing sugars, chlorophylls (a, b and total) and pH during July 2015 whereas total sugars were lowest during December 2015. Proteins and vitamin C values were lowest during August and June 2015 respectively. Sewage and vehicular pollution showed an adverse effect on the nutritional contents of Moringa oleifera leaves. Of the two polluted sites, samples from near the sewage flow showed higher impact.

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