Functional activation of a novel R2R3-MYB protein gene, GmMYB68, confers salt-alkali resistance in soybean (Glycine max L.)

Genome ◽  
2020 ◽  
Vol 63 (1) ◽  
pp. 13-26 ◽  
Author(s):  
Yuxuan He ◽  
Yingshan Dong ◽  
Xiangdong Yang ◽  
Dongquan Guo ◽  
Xueyan Qian ◽  
...  
Keyword(s):  
Glycine Max ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Alkali Resistance ◽  
Myb Transcription Factor ◽  
Pcr Analysis ◽  
Predicted Amino Acid Sequence ◽  
Wild Type ◽  
Glycine Max L ◽  
R2r3 Myb

Soil salinity significantly reduces soybean (Glycine max L.) production worldwide. Plants resistance to stress conditions is a complex characteristic regulated by multiple signaling pathways. The v-Myb avian myeloblastosis viral oncogene homolog (MYB) transcription factor (TF) plays a crucial role in plant development, secondary metabolism, and abiotic stress responses. GmMYB68-overexpression and RNA interference (RNAi) lines were established for examining the function of G. max GmMYB68 in plant responses to abiotic stresses. The predicted amino acid sequence of GmMYB68 was similar to that of R2R3-MYB proteins. Quantitative real-time PCR analysis revealed that GmMYB68 expression varied in response to abiotic stresses. GmMYB68-overexpression lines showed enhanced resistance to salt and alkali stresses and their osmotic adjustment and photosynthetic rates were also stronger than that of GmMYB68-RNAi and wild type plants. Although wild type and transgenic plants showed no significant differences in agronomic traits under normal conditions, the overexpression of GmMYB68 increased grain number and 100-grain weights under salt stress. Our study identified a valuable TF associated with stress response in soybean, as its overexpression might help improve salt and alkali tolerance in soybean and other crops.

scholarly journals Drought Tolerance Conferred in Soybean (Glycine max. L) by GmMYB84, a Novel R2R3-MYB Transcription Factor

2017 ◽  
Vol 58 (10) ◽  
pp. 1764-1776 ◽  
Author(s):  
Nan Wang ◽  
Wenxiao Zhang ◽  
Mengyin Qin ◽  
Shuo Li ◽  
Meng Qiao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Drought Tolerance ◽  
Myb Transcription Factor ◽  
Glycine Max L ◽  
R2r3 Myb

scholarly journals A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6 (Ames1)

2021 ◽  
Author(s):  
Junping Yu ◽  
Guolong Zhao ◽  
Wei Li ◽  
Ying Zhang ◽  
Peng Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Male Sterility ◽  
Bulked Segregant Analysis ◽  
Soybean Protein ◽  
Anther Development ◽  
Hybrid Breeding ◽  
Myb Transcription Factor ◽  
Male Sterile ◽  
R2r3 Myb

Abstract Key message Identification and functional analysis of the male sterile gene MS6 in Glycine max. Abstract Soybean (Glycine max (L.) Merr.) is an important crop providing vegetable oil and protein. The male sterility-based hybrid breeding is a promising method for improving soybean yield to meet the globally growing demand. In this research, we identified a soybean genic male sterile locus, MS6, by combining the bulked segregant analysis sequencing method and the map-based cloning technology. MS6, highly expressed in anther, encodes an R2R3 MYB transcription factor (GmTDF1-1) that is homologous to Tapetal Development and Function 1, a key factor for anther development in Arabidopsis and rice. In male sterile ms6 (Ames1), the mutant allele contains a missense mutation, leading to the 76th leucine substituted by histidine in the DNA binding domain of GmTDF1-1. The expression of soybean MS6 under the control of the AtTDF1 promoter could rescue the male sterility of attdf1 but ms6 could not. Additionally, ms6 overexpression in wild-type Arabidopsis did not affect anther development. These results evidence that GmTDF1-1 is a functional TDF1 homolog and L76H disrupts its function. Notably, GmTDF1-1 shows 92% sequence identity with another soybean protein termed as GmTDF1-2, whose active expression also restored the fertility of attdf1. However, GmTDF1-2 is constitutively expressed at a very low level in soybean, and therefore, not able to compensate for the MS6 deficiency. Analysis of the TDF1-involved anther development regulatory pathway showed that expressions of the genes downstream of TDF1 are significantly suppressed in ms6, unveiling that GmTDF1-1 is a core transcription factor regulating soybean anther development.

scholarly journals Genome-wide identification and expression analysis of the VQ gene family in soybean (Glycine max)

2019 ◽  
Author(s):  
Yongbin Wang ◽  
Zhenfeng Jiang ◽  
Zhenxiang Li ◽  
Yuanling Zhao ◽  
Weiwei Tan ◽  
...  
Keyword(s):  
Glycine Max ◽  
Plant Growth ◽  
Gene Family ◽  
Stress Responses ◽  
Pcr Analysis ◽  
Rna Seq ◽  
Biological Functions ◽  
Conserved Domain ◽  
Genome Wide ◽  
Development Processes

Background. VQ proteins, the plant-specific transcription factors, are involved in the regulation of plant growth, development, and stress responses; however, few articles systematic reported VQ genes in the soybean. Methods. In total, we identified 75 GmVQ genes, which were classified into 7 groups (Ⅰ-Ⅶ). Conserved domain analysis indicated that VQ gene family members all contained the VQ domains. The VQ genes from the same evolutionary branches of soybean shared similar motifs and structures. Promoter analysis revealed cis-elements related to stress responses, phytohormone responses and controlling physical and reproductive growth. Based on the RNA-seq and qRT-PCR analysis, GmVQ genes were expressed in nine tissues suggested their putative function in many aspects of plant growth and development, and response to stresses in Glycine max. Results. The present study provided basic information for further analysis of the biological functions of GmVQ proteins in various development processes.

scholarly journals Genome-Wide Analysis of the MYB Transcription Factor Superfamily in Physcomitrella patens

2020 ◽  
Vol 21 (3) ◽  
pp. 975 ◽  
Author(s):  
Xiaojun Pu ◽  
Lixin Yang ◽  
Lina Liu ◽  
Xiumei Dong ◽  
Silin Chen ◽  
...  
Keyword(s):  
Stress Responses ◽  
Physcomitrella Patens ◽  
Myb Transcription Factor ◽  
Rna Seq ◽  
Genome Wide ◽  
Terrestrial Environments ◽  
Gene Structures ◽  
Myb Genes ◽  
Myb Proteins ◽  
R2r3 Myb

MYB transcription factors (TFs) are one of the largest TF families in plants to regulate numerous biological processes. However, our knowledge of the MYB family in Physcomitrella patens is limited. We identified 116 MYB genes in the P. patens genome, which were classified into the R2R3-MYB, R1R2R3-MYB, 4R-MYB, and MYB-related subfamilies. Most R2R3 genes contain 3 exons and 2 introns, whereas R1R2R3 MYB genes contain 10 exons and 9 introns. N3R-MYB (novel 3RMYB) and NR-MYBs (novel RMYBs) with complicated gene structures appear to be novel MYB proteins. In addition, we found that the diversity of the MYB domain was mainly contributed by domain shuffling and gene duplication. RNA-seq analysis suggested that MYBs exhibited differential expression to heat and might play important roles in heat stress responses, whereas CCA1-like MYB genes might confer greater flexibility to the circadian clock. Some R2R3-MYB and CCA1-like MYB genes are preferentially expressed in the archegonium and during the transition from the chloronema to caulonema stage, suggesting their roles in development. Compared with that of algae, the numbers of MYBs have significantly increased, thus our study lays the foundation for further exploring the potential roles of MYBs in the transition from aquatic to terrestrial environments.

scholarly journals Nitrate Reductases from Wild-Type and nr1-Mutant Soybean (Glycine max [L.] Merr.) Leaves

1985 ◽  
Vol 78 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Luc Streit ◽  
Richard S. Nelson ◽  
James E. Harper
Keyword(s):  
Glycine Max ◽  
Wild Type ◽  
Glycine Max L ◽  
Nitrate Reductases

scholarly journals Nitrate Reductases from Wild-Type and nr1-Mutant Soybean (Glycine max [L.] Merr.) Leaves

1986 ◽  
Vol 80 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Richard S. Nelson ◽  
Luc Streit ◽  
James E. Harper
Keyword(s):  
Glycine Max ◽  
Wild Type ◽  
Glycine Max L ◽  
Nitrate Reductases

scholarly journals MdMYB4, an R2R3-Type MYB Transcription Factor, Plays a Crucial Role in Cold and Salt Stress in Apple Calli

2017 ◽  
Vol 142 (3) ◽  
pp. 209-216 ◽  
Author(s):  
Ruigang Wu ◽  
Yi Wang ◽  
Ting Wu ◽  
Xuefeng Xu ◽  
Zhenhai Han
Keyword(s):  
Salt Stress ◽  
Cold Stress ◽  
Stress Responses ◽  
Transcriptional Activation ◽  
Myb Transcription Factor ◽  
Abiotic Stress Response ◽  
Physiological Processes ◽  
In The Wild ◽  
Polymerase Chain ◽  
R2r3 Myb

MYB (v-myb avian myeloblastosis viral oncogene homologs) transcription factors (TFs) are involved in diverse physiological processes, including cell shape determination, cell differentiation, and secondary metabolism, as well as abiotic stress response. In the present study, MdMYB4, an R2R3-MYB protein that is a homolog of Arabidopsis thaliana MYB4, was identified and characterized. Quantitative real-time polymerase chain reaction (qRT-PCR) expression analysis demonstrated that MdMYB4 is extensively expressed in various apple (Malus domestica) tissues and that its expression is induced by cold, osmotic, and salt stress. An MdMYB4-GFP fusion protein was localized in the nucleus of transformed onion (Allium cepa) epidermal cells and had a certain transcriptional activation activity by yeast one-hybrid assay. Overexpression of the MdMYB4 gene remarkably enhanced the tolerance of stably transgenic apple calli to severe salt and cold stress, and both the relative conductivity and malondialdehyde (MDA) accumulation of transgenic calli under salt and cold stress were significantly lower than in the wild type control. Taken together, these results suggest that MdMYB4 may play a positive regulatory role in both cold and salt stress responses.

scholarly journals An R2R3-MYB Transcription Factor PgFLP Directly Activates PgPIN10 to Regulate the GSA of Adventitious Root in Pomegranate

2020 ◽  
Author(s):  
Zenghui Wang ◽  
Jialin Li ◽  
Xuemei Yang ◽  
Haixia Tang ◽  
Lijuan Feng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Adventitious Root ◽  
Molecular Mechanisms ◽  
Lateral Roots ◽  
Myb Transcription Factor ◽  
Wild Type ◽  
Set Point ◽  
Root Gravitropism ◽  
Gravity Signal ◽  
R2r3 Myb

Abstract Background: The self-rooted seedling is widely used in pomegranate planting industry currently; However, the root system of self-rooted seedling is shallow and poor cold resistance. Therefore, the study of the molecular mechanisms of pomegranate adventitious root gravitropism is very important for developing deep-rooted pomegranate cultivars.Results: We report the pomegranate FOUR LIPS (PgFLP) that play an key role in regulating the gravitropic set-point angle of pomegranate adventitious root in response to gravity signal. In our study, PgFLP directly regulates the transcriptional expression of PgPIN10 by binding to its promoter, thus regulating the GSA of adventitious root in pomegranate. Additionally, the 35S::PgFLP show stronger gravitational response than wild-type, leading to a smaller GSA in Arabidopsis lateral roots, indicating that PgFLP participates in regulating the GSA of adventitious root via PgPIN10 in pomegranate. Conclusion: Our results confirm that the transcriptional regulation of PgPIN10 by R2R3-MYB transcription factor PgFLP in setting the gravitropic set-point angle of pomegranate adventitious root in response to gravity signal.

scholarly journals Heat Stress Tolerance Gene FpHsp104 Affects Conidiation and Pathogenicity of Fusarium pseudograminearum

2021 ◽  
Vol 12 ◽  
Author(s):  
Huiqing Xia ◽  
Linlin Chen ◽  
Zhuo Fan ◽  
Mengya Peng ◽  
Jingya Zhao ◽  
...  
Keyword(s):  
Heat Shock ◽  
Stress Responses ◽  
Fungal Pathogens ◽  
Functional Characterization ◽  
Wall Stress ◽  
Pcr Analysis ◽  
Wild Type ◽  
Fusarium Pseudograminearum ◽  
Heat Stress Tolerance ◽  
Tolerance Gene

Heat shock protein Hsp104, a homolog of the bacterial chaperone ClpB and plant Hsp100, plays an essential part in the response to heat and various chemical agents in Saccharomyces cerevisiae. However, their functions remain largely unknown in plant fungal pathogens. Here, we report the identification and functional characterization of a plausible ortholog of yeast Hsp104 in Fusarium pseudograminearum, which we termed FpHsp104. Deletion mutant of FpHsp104 displayed severe defects in the resistance of heat shock during F. pseudograminearum mycelia and conidia when exposed to extreme heat. We also found that the protein showed dynamic localization to small particles under high temperature. However, no significant differences were detected in osmotic, oxidative, or cell wall stress responses between the wild-type and Δfphsp104 strains. Quantitative real-time PCR analysis showed that FpHsp104 was upregulated in the conidia, and disruption of FpHsp104 gene resulted in defects in conidia production, morphology, and germination. The transcript levels of conidiation-related genes of FpFluG, FpVosA, FpWetA, and FpAbaA were reduced in the Δfphsp104 mutant vs. the wild-type strain, but heat-shocked mRNA splicing repair was not affected in Δfphsp104. Moreover, Δfphsp104 mutant also showed attenuated virulence, but its DON synthesis was normal. These data from the first study of Hsp104 in F. pseudograminearum strongly suggest that FpHsp104 gene is an important element in the heat tolerance, development, and pathogenicity processes of F. pseudograminearum.

scholarly journals NtMYB3, an R2R3-MYB from Narcissus, Regulates Flavonoid Biosynthesis

2019 ◽  
Vol 20 (21) ◽  
pp. 5456 ◽  
Author(s):  
Muhammad Anwar ◽  
Weijun Yu ◽  
Hong Yao ◽  
Ping Zhou ◽  
Andrew C. Allan ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Flavonoid Biosynthesis ◽  
Pcr Analysis ◽  
Wild Type ◽  
Narcissus Tazetta ◽  
Over Expression ◽  
Qrt Pcr ◽  
Red Color ◽  
R2r3 Myb ◽  
R2r3 Myb Transcription Factors

R2R3-MYB transcription factors play important roles in the regulation of plant flavonoid metabolites. In the current study, NtMYB3, a novel R2R3-MYB transcriptional factor isolated from Chinese narcissus (Narcissus tazetta L. var. chinensis), was functionally characterized. Phylogenetic analysis indicated that NtMYB3 belongs to the AtMYB4-like clade, which includes repressor MYBs involved in the regulation of flavonoid biosynthesis. Transient assays showed that NtMYB3 significantly reduced red pigmentation induced by the potato anthocyanin activator StMYB-AN1 in agro-infiltrated leaves of tobacco. Over-expression of NtMYB3 decreased the red color of transgenic tobacco flowers, with qRT-PCR analysis showing that NtMYB3 repressed the expression levels of genes involved in anthocyanin and flavonol biosynthesis. However, the proanthocyanin content in flowers of transgenic tobacco increased as compared to wild type. NtMYB3 showed expression in all examined narcissus tissues; the expression level in basal plates of the bulb was highest. A 968 bp promoter fragment of narcissus FLS (NtFLS) was cloned, and transient expression and dual luciferase assays showed NtMYB3 repressed the promoter activity. These results reveal that NtMYB3 is involved in the regulation of flavonoid biosynthesis in narcissus by repressing the biosynthesis of flavonols, and this leads to proanthocyanin accumulation in the basal plate of narcissus.

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