scholarly journals Drought Tolerance Conferred in Soybean (Glycine max. L) by GmMYB84, a Novel R2R3-MYB Transcription Factor

2017 ◽  
Vol 58 (10) ◽  
pp. 1764-1776 ◽  
Author(s):  
Nan Wang ◽  
Wenxiao Zhang ◽  
Mengyin Qin ◽  
Shuo Li ◽  
Meng Qiao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Drought Tolerance ◽  
Myb Transcription Factor ◽  
Glycine Max L ◽  
R2r3 Myb

scholarly journals A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6 (Ames1)

2021 ◽  
Author(s):  
Junping Yu ◽  
Guolong Zhao ◽  
Wei Li ◽  
Ying Zhang ◽  
Peng Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Male Sterility ◽  
Bulked Segregant Analysis ◽  
Soybean Protein ◽  
Anther Development ◽  
Hybrid Breeding ◽  
Myb Transcription Factor ◽  
Male Sterile ◽  
R2r3 Myb

Abstract Key message Identification and functional analysis of the male sterile gene MS6 in Glycine max. Abstract Soybean (Glycine max (L.) Merr.) is an important crop providing vegetable oil and protein. The male sterility-based hybrid breeding is a promising method for improving soybean yield to meet the globally growing demand. In this research, we identified a soybean genic male sterile locus, MS6, by combining the bulked segregant analysis sequencing method and the map-based cloning technology. MS6, highly expressed in anther, encodes an R2R3 MYB transcription factor (GmTDF1-1) that is homologous to Tapetal Development and Function 1, a key factor for anther development in Arabidopsis and rice. In male sterile ms6 (Ames1), the mutant allele contains a missense mutation, leading to the 76th leucine substituted by histidine in the DNA binding domain of GmTDF1-1. The expression of soybean MS6 under the control of the AtTDF1 promoter could rescue the male sterility of attdf1 but ms6 could not. Additionally, ms6 overexpression in wild-type Arabidopsis did not affect anther development. These results evidence that GmTDF1-1 is a functional TDF1 homolog and L76H disrupts its function. Notably, GmTDF1-1 shows 92% sequence identity with another soybean protein termed as GmTDF1-2, whose active expression also restored the fertility of attdf1. However, GmTDF1-2 is constitutively expressed at a very low level in soybean, and therefore, not able to compensate for the MS6 deficiency. Analysis of the TDF1-involved anther development regulatory pathway showed that expressions of the genes downstream of TDF1 are significantly suppressed in ms6, unveiling that GmTDF1-1 is a core transcription factor regulating soybean anther development.

scholarly journals A R2R3-MYB Transcription Factor Gene, BpMYB123, Regulates BpLEA14 to Improve Drought Tolerance in Betula platyphylla

2021 ◽  
Vol 12 ◽  
Author(s):  
Kaiwen Lv ◽  
Hairong Wei ◽  
Guifeng Liu
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Crop Production ◽  
Myb Transcription Factor ◽  
Betula Platyphylla ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Negative Impacts ◽  
R2r3 Myb

Drought stress causes various negative impacts on plant growth and crop production. R2R3-MYB transcription factors (TFs) play crucial roles in the response to abiotic stress. However, their functions in Betula platyphylla haven’t been fully investigated. In this study, a R2R3 MYB transcription factor gene, BpMYB123, was identified from Betula platyphylla and reveals its significant role in drought stress. Overexpression of BpMYB123 enhances tolerance to drought stress in contrast to repression of BpMYB123 by RNA interference (RNAi) in transgenic experiment. The overexpression lines increased peroxidase (POD) and superoxide dismatase (SOD) activities, while decreased hydrogen peroxide (H2O2), superoxide radicals (O2–), electrolyte leakage (EL) and malondialdehyde (MDA) contents. Our study showed that overexpression of BpMYB123 increased BpLEA14 gene expression up to 20-fold due to BpMYB123 directly binding to the MYB1AT element of BpLEA14 promoter. These results indicate that BpMYB123 acts as a regulator via regulating BpLEA14 to improve drought tolerance in birch.

scholarly journals Loss-of-function mutations affecting a specific Glycine max R2R3 MYB transcription factor result in brown hilum and brown seed coats

2011 ◽  
Vol 11 (1) ◽  
pp. 155 ◽  
Author(s):  
Jason D Gillman ◽  
Ashley Tetlow ◽  
Jeong-Deong Lee ◽  
J Shannon ◽  
Kristin Bilyeu
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Myb Transcription Factor ◽  
Loss Of Function ◽  
R2r3 Myb ◽  
Seed Coats

scholarly journals R2R3-MYB Transcription Factor PlMYB108 Confers Drought Tolerance in Herbaceous Peony (Paeonia lactiflora Pall.)

2021 ◽  
Vol 22 (21) ◽  
pp. 11884
Author(s):  
Yanqing Wu ◽  
Tingting Li ◽  
Zhuoya Cheng ◽  
Daqiu Zhao ◽  
Jun Tao
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Myb Transcription Factor ◽  
Leaf Water Content ◽  
Antioxidant Enzyme Activities ◽  
Paeonia Lactiflora ◽  
Ros Scavenging ◽  
Herbaceous Peony ◽  
Tf Gene ◽  
R2r3 Myb

The MYB transcription factor (TF) is crucial for plant growth, development, and response to abiotic stress, but it is rarely reported in the herbaceous peony (Paeonia lactiflora Pall.). Here, an MYB TF gene was isolated, and based on our prior mRNA data from P. lactiflora samples, it was treated with drought stress (DS). Its complete cDNA structure was 1314 bp, which encoded 291 amino acids (aa). Furthermore, using sequence alignment analysis, we demonstrated that PlMYB108 was an R2R3-MYB TF. We also revealed that PlMYB108 was primarily localized in the nucleus. Its levels rose during DS, and it was positively correlated with drought tolerance (DT) in P. lactiflora. In addition, when PlMYB108 was overexpressed in tobacco plants, the flavonoid content, antioxidant enzyme activities, and photosynthesis were markedly elevated. Hence, the transgenic plants had stronger DT with a higher leaf water content and lower H2O2 accumulation compared to the wild-type (WT) plants. Based on these results, PlMYB108 is a vital gene that serves to increase flavonoid accumulation, reactive oxygen species (ROS), scavenging capacity, and photosynthesis to confer DT. The results would provide a genetic resource for molecular breeding to enhance plant DT.

Functional activation of a novel R2R3-MYB protein gene, GmMYB68, confers salt-alkali resistance in soybean (Glycine max L.)

Genome ◽  
2020 ◽  
Vol 63 (1) ◽  
pp. 13-26 ◽  
Author(s):  
Yuxuan He ◽  
Yingshan Dong ◽  
Xiangdong Yang ◽  
Dongquan Guo ◽  
Xueyan Qian ◽  
...  
Keyword(s):  
Glycine Max ◽  
Stress Responses ◽  
Abiotic Stresses ◽  
Alkali Resistance ◽  
Myb Transcription Factor ◽  
Pcr Analysis ◽  
Predicted Amino Acid Sequence ◽  
Wild Type ◽  
Glycine Max L ◽  
R2r3 Myb

Soil salinity significantly reduces soybean (Glycine max L.) production worldwide. Plants resistance to stress conditions is a complex characteristic regulated by multiple signaling pathways. The v-Myb avian myeloblastosis viral oncogene homolog (MYB) transcription factor (TF) plays a crucial role in plant development, secondary metabolism, and abiotic stress responses. GmMYB68-overexpression and RNA interference (RNAi) lines were established for examining the function of G. max GmMYB68 in plant responses to abiotic stresses. The predicted amino acid sequence of GmMYB68 was similar to that of R2R3-MYB proteins. Quantitative real-time PCR analysis revealed that GmMYB68 expression varied in response to abiotic stresses. GmMYB68-overexpression lines showed enhanced resistance to salt and alkali stresses and their osmotic adjustment and photosynthetic rates were also stronger than that of GmMYB68-RNAi and wild type plants. Although wild type and transgenic plants showed no significant differences in agronomic traits under normal conditions, the overexpression of GmMYB68 increased grain number and 100-grain weights under salt stress. Our study identified a valuable TF associated with stress response in soybean, as its overexpression might help improve salt and alkali tolerance in soybean and other crops.

scholarly journals Different Mechanisms Participate in the R-dependent Activity of the R2R3 MYB Transcription Factor C1

2004 ◽  
Vol 279 (46) ◽  
pp. 48205-48213 ◽  
Author(s):  
J. Marcela Hernandez ◽  
George F. Heine ◽  
Niloufer G. Irani ◽  
Antje Feller ◽  
Min-Gab Kim ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Myb Transcription Factor ◽  
Dependent Activity ◽  
R2r3 Myb
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