Isolation and molecular cytogenetic characterization of a wheat – Leymus mollis double monosomic addition line and its progenies with resistance to stripe rust

Genome ◽  
2017 ◽  
Vol 60 (12) ◽  
pp. 1029-1036 ◽  
Author(s):  
Xiaofei Yang ◽  
Xin Li ◽  
Changyou Wang ◽  
Chunhuan Chen ◽  
Zengrong Tian ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Addition Line ◽  
Cytogenetic Characterization ◽  
Monosomic Addition ◽  
Monosomic Addition Line ◽  
Leymus Mollis

A common wheat – Leymus mollis (2n = 4x = 28, NsNsXmXm) double monosomic addition line, M11003-4-3-8/13/15 (2n = 44 = 42T.a + L.m2 + L.m3), with stripe rust resistance was developed (where T.a represents Triticum aestivum chromosome, L.m represents L. mollis chromosome, and L.m2/3 represents L. mollis chromosome of homoeologous groups 2 and 3). The progenies of line M11003-4-3-8/13/15 were characterized by cytological observation, specific molecular markers, fluorescence in situ hybridization (FISH), and genomic in situ hybridization (GISH). Among the progenies, there existed five different types (I, II, III, IV, and V) of chromosome constitution, the formulas of which were 2n = 44 = 42T.a + 1L.m2 + 1L.m3, 2n = 43 = 42T.a + 1L.m2, 2n = 43 = 42T.a + 1L.m3, 2n = 42 = 42T.a, and 2n = 44 = 42T.a + 2L.m2, respectively. Field disease screening showed that types I and III showed high resistance to stripe rust, while types II, IV, and V were susceptible. Leymus mollis was almost immune to stripe rust, whereas the wheat parent, cultivar 7182, was susceptible. Therefore, we concluded that the stripe rust resistance originated from L. mollis. These various lines could be further fully exploited as important disease resistance materials to enrich wheat genetic resources.

scholarly journals Molecular cytogenetics identification of a wheat – Leymus mollis double disomic addition line with stripe rust resistance

Genome ◽  
2017 ◽  
Vol 60 (5) ◽  
pp. 375-383 ◽  
Author(s):  
Aicen Zhang ◽  
Wanyue Li ◽  
Changyou Wang ◽  
Xiaofei Yang ◽  
Chunhuan Chen ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Common Wheat ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Fish Analysis ◽  
Addition Line ◽  
Disomic Addition Line ◽  
Leymus Mollis

Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) possesses a number of valuable genes against biotic and abiotic stress, which could be transferred into common wheat background for wheat improvement. In the present study, we determined the karyotypic constitution of a wheat – L. mollis double disomic addition line, M11003-4-4-1-1, selected from the F5 progeny of a stable wheat – L. mollis derivative M39 (2n = 56) × Triticum aestivum cultivar 7182, by morphological and cytogenetic identification, GISH (genomic in situ hybridization), FISH (fluorescent in situ hybridization), molecular markers analysis, and stripe rust resistance evaluation. Cytological studies demonstrated that M11003-4-4-1-1 had a chromosome karyotype of 2n = 46 with 23 bivalents, while GISH and FISH analysis indicated that this line contained 42 common wheat chromosomes and two pairs of L. mollis chromosomes. DNA markers showed that the alien chromosomes from L. mollis belonged to homoeologous groups 5 and 6. Evaluation of the agronomic traits revealed that M11003-4-4-1-1 was resistant to stripe rust at the adult stage, while the plant height was reduced and the 1000-grain weight was increased significantly. Therefore, the new line M11003-4-4-1-1 could be exploited as an important bridge material in chromosome engineering and wheat breeding.

scholarly journals Molecular cytogenetic identification of a wheat – Thinopyrum ponticum substitution line with stripe rust resistance

Genome ◽  
2017 ◽  
Vol 60 (10) ◽  
pp. 860-867 ◽  
Author(s):  
Chen Zhu ◽  
Yanzhen Wang ◽  
Chunhuan Chen ◽  
Changyou Wang ◽  
Aicen Zhang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Stripe Rust ◽  
Expressed Sequence Tag ◽  
Wheat Breeding ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Fish Analysis ◽  
Thinopyrum Ponticum ◽  
Expressed Sequence

Thinopyrum ponticum (Th. ponticum) (2n = 10x = 70) is an important breeding material with excellent resistance and stress tolerance. In this study, we characterized the derivative line CH1113-B13-1-1-2-1 (CH1113-B13) through cytological, morphological, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), expressed sequence tag (EST), and PCR-based landmark unique gene (PLUG) marker analysis. The GISH analysis revealed that CH1113-B13 contained 20 pairs of common wheat chromosomes and one pair of JSt genomic chromosomes. Linkage analysis of Th. ponticum using seven EST and seven PLUG markers indicated that the pair of alien chromosomes belonged to the seventh homeologous group. Nulli-tetrasomic and FISH analysis revealed that wheat 7B chromosomes were absent in CH1113-B13; thus, CH1113-B13 was identified as a 7JSt (7B) substitution line. Finally, adult-stage CH1113-B13 exhibited immunity to wheat stripe rust. This substitution line is therefore a promising germplasm resource for wheat breeding.

scholarly journals Development and Cytomolecular Identification of Monosomic Alien Addition and Substitution Lines of Triticale (×Triticosecale Wittmack) With 2Sk Chromosome Conferring Leaf Rust Resistance Derived From Aegilops kotschyi Boiss

2020 ◽  
Vol 11 ◽  
Author(s):  
Michał T. Kwiatek ◽  
Waldemar Ulaszewski ◽  
Jolanta Belter ◽  
Dylan Phillips ◽  
Roksana Skowrońska ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Stripe Rust Resistance ◽  
Chromosome Engineering ◽  
Substitution Lines ◽  
Starting Point ◽  
Monosomic Addition ◽  
Triticosecale Wittmack

Alien chromosome introgression has become a valuable tool to broaden the genetic variability of crop plants via chromosome engineering. This study details the procedure to obtain monosomic addition and monosomic substitution lines of the triticale carrying 2Sk chromosome from Aegilops kotchyi Boiss., which harbors Lr54 + Yr37 leaf and stripe rust-resistant gene loci, respectively. Initially, A. kotschyi × Secale cereale artificial amphiploids (2n = 6x = 42 chromosomes, UUSSRR) were crossed with triticale cv. “Sekundo” (2n = 6x = 42, AABBRR) in order to obtain fertile offspring. Cyto-molecular analyses of five subsequent backcrossing generations revealed that 2Sk chromosome was preferentially transmitted. This allowed for the selection of monosomic 2Sk addition (MA2Sk) lines of triticale. Finally, the 2Sk(2R) substitution plants were obtained by crossing MA2Sk with the nullisomic (N2R) plants of triticale. The presence of 2Sk chromosome in subsequent generations of plants was evaluated using SSR markers linked to Lr54 + Yr37 loci. Disease evaluation of the monosomic 2Sk(2R) substitution plants for the reaction to leaf and stripe rust infection were carried out under controlled conditions in a growth chamber. The results showed significant improvement of leaf rust resistance severity of monosomic substitution plants compared with control (“Sekundo”). In contrast, the introgression of the Lr54 + Yr37 loci did not lead to improvement of stripe rust resistance. In summary, the creation of monosomic addition and monosomic substitution lines of triticale is the starting point for the precise and guided transfer of Lr54 + Yr37 loci. The results showed that the developed materials could be exploited for the development of triticale varieties with resistance to leaf rust.

scholarly journals Molecular cytogenetics and St-chromosome-specific molecular markers development of novel stripe rust resistant wheat–Thinopyrum intermedium as well as wheat–Thinopyrum ponticum substitution lines

2021 ◽  
Author(s):  
Siwen Wang ◽  
Changyou Wang ◽  
Xianbo Feng ◽  
Jixin Zhao ◽  
Pingchuan Deng ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Snp Array ◽  
Kernel Weight ◽  
Stripe Rust Resistance ◽  
Substitution Lines ◽  
Fish Genome ◽  
Thinopyrum Ponticum

Abstract Background Owing to the excellent resistance to abiotic and biotic stress, Thionpyrum intermedium (2n = 6x = 42, JJJsJsStSt) and Thinopyrum ponticum (2n = 10x = 70) are both widely utilized in wheat germplasm innovation programs. Disomic substitution lines (DSLs) carrying one pair of alien chromosomes are valuable bridge materials for novel genes transmission, FISH karyotype construction and specific molecular marker development. Results Six wheat–Thinopyrum DSLs derived from crosses between Abbondanza nullisomic lines (2n = 40) and two octoploid Trititrigia lines (2n = 8x = 56), were characterized by a sequential fluorescence in situ hybridization (FISH)–genome in situ hybridization (GISH), a multicolor GISH (mc-GISH), and an analysis of wheat 15K SNP array combined with molecular marker selection. ES-9 (DS2St (2A)) and ES-10 (DS3St (3D)) are wheat–Th. ponticum DSLs, while ES-23 (DS2St (2A)), ES-24 (DS3St (3D)), ES-25(DS2St (2B)), and ES-26 (DS2St (2D)) are wheat–Th. intermedium DSLs. ES-9, ES-23, ES-25 and ES-26 conferred higher thousand-kernel weight and stripe rust resistance at adult stages, while ES-10 and ES-24 performed highly resistant to stripe rust at all stages. Furthermore, cytological analysis showed that the alien chromosomes (2St/3St) belonging to the same homoeologous group derived from different donors carried the same FISH karyotype and could normally form a bivalent. Based on specific-locus amplified fragment sequencing (SLAF-seq), two 2St-chromosome-specific markers (PTH-005 and PTH-013) and two 3St-chromosome-specific markers (PTH-113 and PTH-135) were developed. Conclusions The six wheat–Thinopyrum disomic substitution lines conferring stripe rust resistance will be used as bridging parents for valuable resistant genes transmission. And the utility of PTH-113 and PTH-135 in a BC1F2 population showed the newly developed markers could be useful tools for efficient identification of St chromosomes in a common wheat background.

Characterization of New Wheat-Dasypyrum breviaristatum Introgression Lines with Superior Gene(s) for Spike Length and Stripe Rust Resistance

2018 ◽  
Vol 156 (2) ◽  
pp. 117-125 ◽  
Author(s):  
Hongjin Wang ◽  
Zhihui Yu ◽  
Bin Li ◽  
Tao Lang ◽  
Guangrong Li ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Field Evaluation ◽  
Spike Length ◽  
Stripe Rust Resistance ◽  
Stripe Rust Resistance Gene ◽  
Fraction Length ◽  
Dasypyrum Breviaristatum

Dasypyrum breviaristatum (genome VbVb) contains potentially important traits for commercial wheat production. Chromosome 2Vb of D. breviaristatum carries several desirable agronomic characters, including long spike length as well as enhanced resistance to stripe rust, which are expressed in a common wheat background. In this study, wheat-D. breviaristatum 2Vb deletion lines were produced and identified by fluorescence in situ hybridization (FISH), and 74 molecular markers specific to D. breviaristatum chromosome 2Vb were physically localized in 4 distinct chromosomal regions. New wheat-D. breviaristatum 2Vb translocation lines were also characterized by FISH. The breakpoint of the translocation T3AS.3AL-2VbS was determined by physically mapped molecular markers. Field evaluation revealed that genes affecting plant height and spike length are located on fraction length (FL) 0.65-1.00 of 2VbS, while the stripe rust resistance gene(s) are located on FL 0.40-1.00 of D. breviaristatum chromosome 2VbL. The newly characterized wheat-Dasypyrum chromosomal introgressions are of potential value for the improvement of the yield and disease resistance of wheat.

scholarly journals Mining Centuries Old In situ Conserved Turkish Wheat Landraces for Grain Yield and Stripe Rust Resistance Genes

2016 ◽  
Vol 7 ◽  
Author(s):  
Deepmala Sehgal ◽  
Susanne Dreisigacker ◽  
Savaş Belen ◽  
Ümran Küçüközdemir ◽  
Zafer Mert ◽  
...  
Keyword(s):  
Grain Yield ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Wheat Landraces ◽  
Rust Resistance Genes
Sign in / Sign up

Export Citation Format

Share Document