scholarly journals Molecular cytogenetics identification of a wheat – Leymus mollis double disomic addition line with stripe rust resistance

Genome ◽  
2017 ◽  
Vol 60 (5) ◽  
pp. 375-383 ◽  
Author(s):  
Aicen Zhang ◽  
Wanyue Li ◽  
Changyou Wang ◽  
Xiaofei Yang ◽  
Chunhuan Chen ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Common Wheat ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Fish Analysis ◽  
Addition Line ◽  
Disomic Addition Line ◽  
Leymus Mollis

Leymus mollis (Trin.) Pilg. (2n = 4x = 28, NsNsXmXm) possesses a number of valuable genes against biotic and abiotic stress, which could be transferred into common wheat background for wheat improvement. In the present study, we determined the karyotypic constitution of a wheat – L. mollis double disomic addition line, M11003-4-4-1-1, selected from the F5 progeny of a stable wheat – L. mollis derivative M39 (2n = 56) × Triticum aestivum cultivar 7182, by morphological and cytogenetic identification, GISH (genomic in situ hybridization), FISH (fluorescent in situ hybridization), molecular markers analysis, and stripe rust resistance evaluation. Cytological studies demonstrated that M11003-4-4-1-1 had a chromosome karyotype of 2n = 46 with 23 bivalents, while GISH and FISH analysis indicated that this line contained 42 common wheat chromosomes and two pairs of L. mollis chromosomes. DNA markers showed that the alien chromosomes from L. mollis belonged to homoeologous groups 5 and 6. Evaluation of the agronomic traits revealed that M11003-4-4-1-1 was resistant to stripe rust at the adult stage, while the plant height was reduced and the 1000-grain weight was increased significantly. Therefore, the new line M11003-4-4-1-1 could be exploited as an important bridge material in chromosome engineering and wheat breeding.

Isolation and molecular cytogenetic characterization of a wheat – Leymus mollis double monosomic addition line and its progenies with resistance to stripe rust

Genome ◽  
2017 ◽  
Vol 60 (12) ◽  
pp. 1029-1036 ◽  
Author(s):  
Xiaofei Yang ◽  
Xin Li ◽  
Changyou Wang ◽  
Chunhuan Chen ◽  
Zengrong Tian ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Addition Line ◽  
Cytogenetic Characterization ◽  
Monosomic Addition ◽  
Monosomic Addition Line ◽  
Leymus Mollis

A common wheat – Leymus mollis (2n = 4x = 28, NsNsXmXm) double monosomic addition line, M11003-4-3-8/13/15 (2n = 44 = 42T.a + L.m2 + L.m3), with stripe rust resistance was developed (where T.a represents Triticum aestivum chromosome, L.m represents L. mollis chromosome, and L.m2/3 represents L. mollis chromosome of homoeologous groups 2 and 3). The progenies of line M11003-4-3-8/13/15 were characterized by cytological observation, specific molecular markers, fluorescence in situ hybridization (FISH), and genomic in situ hybridization (GISH). Among the progenies, there existed five different types (I, II, III, IV, and V) of chromosome constitution, the formulas of which were 2n = 44 = 42T.a + 1L.m2 + 1L.m3, 2n = 43 = 42T.a + 1L.m2, 2n = 43 = 42T.a + 1L.m3, 2n = 42 = 42T.a, and 2n = 44 = 42T.a + 2L.m2, respectively. Field disease screening showed that types I and III showed high resistance to stripe rust, while types II, IV, and V were susceptible. Leymus mollis was almost immune to stripe rust, whereas the wheat parent, cultivar 7182, was susceptible. Therefore, we concluded that the stripe rust resistance originated from L. mollis. These various lines could be further fully exploited as important disease resistance materials to enrich wheat genetic resources.

scholarly journals Molecular cytogenetic identification of a wheat – Thinopyrum ponticum substitution line with stripe rust resistance

Genome ◽  
2017 ◽  
Vol 60 (10) ◽  
pp. 860-867 ◽  
Author(s):  
Chen Zhu ◽  
Yanzhen Wang ◽  
Chunhuan Chen ◽  
Changyou Wang ◽  
Aicen Zhang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Stripe Rust ◽  
Expressed Sequence Tag ◽  
Wheat Breeding ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Fish Analysis ◽  
Thinopyrum Ponticum ◽  
Expressed Sequence

Thinopyrum ponticum (Th. ponticum) (2n = 10x = 70) is an important breeding material with excellent resistance and stress tolerance. In this study, we characterized the derivative line CH1113-B13-1-1-2-1 (CH1113-B13) through cytological, morphological, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), expressed sequence tag (EST), and PCR-based landmark unique gene (PLUG) marker analysis. The GISH analysis revealed that CH1113-B13 contained 20 pairs of common wheat chromosomes and one pair of JSt genomic chromosomes. Linkage analysis of Th. ponticum using seven EST and seven PLUG markers indicated that the pair of alien chromosomes belonged to the seventh homeologous group. Nulli-tetrasomic and FISH analysis revealed that wheat 7B chromosomes were absent in CH1113-B13; thus, CH1113-B13 was identified as a 7JSt (7B) substitution line. Finally, adult-stage CH1113-B13 exhibited immunity to wheat stripe rust. This substitution line is therefore a promising germplasm resource for wheat breeding.

scholarly journals Stripe rust resistance gene Yr34 (synonym Yr48) is located within a distal translocation of Triticum monococcum chromosome 5AmL into common wheat

2021 ◽  
Author(s):  
Shisheng Chen ◽  
Joshua Hegarty ◽  
Tao Shen ◽  
Lei Hua ◽  
Hongna Li ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Common Wheat ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Triticum Monococcum ◽  
Rust Resistance Gene ◽  
Stripe Rust Resistance ◽  
Polyploid Wheat ◽  
Stripe Rust Resistance Gene ◽  
A Genome

AbstractKey messageThe stripe rust resistance geneYr34 was transferred to polyploid wheat chromosome 5AL from T. monococcumand has been used for over two centuries.Wheat stripe (or yellow) rust, caused by Puccinia striiformis f. sp. tritici (Pst), is currently among the most damaging fungal diseases of wheat worldwide. In this study, we report that the stripe rust resistance gene Yr34 (synonym Yr48) is located within a distal segment of the cultivated Triticum monococcum subsp. monococcum chromosome 5AmL translocated to chromosome 5AL in polyploid wheat. The diploid wheat species Triticum monococcum (genome AmAm) is closely related to T. urartu (donor of the A genome to polyploid wheat) and has good levels of resistance against the stripe rust pathogen. When present in hexaploid wheat, the T. monococcum Yr34 resistance gene confers a moderate level of resistance against virulent Pst races present in California and the virulent Chinese race CYR34. In a survey of 1,442 common wheat genotypes, we identified 5AmL translocations of fourteen different lengths in 17.5% of the accessions, with higher frequencies in Europe than in other continents. The old European wheat variety “Mediterranean” was identified as a putative source of this translocation, suggesting that Yr34 has been used for over 200 years. Finally, we designed diagnostic CAPS and sequenced-based markers that will be useful to accelerate the deployment of Yr34 in wheat breeding programs to improve resistance to this devastating pathogen.

scholarly journals Genome-Wide Association Mapping of Adult-Plant Resistance to Stripe Rust in Common Wheat (Triticum aestivum)

Plant Disease ◽  
2020 ◽  
Vol 104 (8) ◽  
pp. 2174-2180 ◽  
Author(s):  
Fangping Yang ◽  
Jindong Liu ◽  
Ying Guo ◽  
Zhonghu He ◽  
Awais Rasheed ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Genome Wide Association ◽  
Stripe Rust Resistance ◽  
Economic Losses ◽  
Genome Wide ◽  
A Genome

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a globally devastating disease of common wheat (Triticum aestivum L.), resulting in substantial economic losses. To identify effective resistance genes, a genome-wide association study was conducted on 120 common wheat lines from different wheat-growing regions of China using the wheat 90K iSelect SNP array. Seventeen loci were identified, explaining 9.5 to 21.8% of the phenotypic variation. Most of these genes were detected in the A (seven) and B (seven) genomes, with only three in the D genome. Among them, 11 loci were colocated with known resistance genes or quantitative trait loci reported previously, whereas the other six are likely new resistance loci. Annotation of flanking sequences of significantly associated SNPs indicated the presence of three important candidate genes, including E3 ubiquitin-protein ligase, F-box repeat protein, and disease resistance RPP13-like protein. This study increased our knowledge in understanding the genetic architecture for stripe rust resistance and identified wheat varieties with multiple resistance alleles, which are useful for improvement of stripe rust resistance in breeding.

Chromosome constitution and origin analysis in three derivatives of Triticum aestivum – Leymus mollis by molecular cytogenetic identification

Genome ◽  
2014 ◽  
Vol 57 (11/12) ◽  
pp. 583-591 ◽  
Author(s):  
Xiaofei Yang ◽  
Changyou Wang ◽  
Chunhuan Chen ◽  
Hong Zhang ◽  
Zengrong Tian ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Powdery Mildew ◽  
Common Wheat ◽  
Stripe Rust ◽  
Wheat Breeding ◽  
Chromosome Constitution ◽  
Disease Resistances ◽  
Derivatives Of ◽  
Leymus Mollis

Leymus mollis (2n = 4x = 28, NsNsXmXm) is an important tetraploid species in Leymus (Poaceae: Triticeae) and a useful genetic resource for wheat breeding because of the stress tolerance and disease resistance of this species. The development of Triticum aestivum (common wheat) – L. mollis derivatives with desirable genes will provide valuable bridge materials for wheat improvement, especially regarding powdery mildew resistance genes, which are rarely documented in L. mollis. In the present study, three derivatives of common wheat cultivar 7182 and L. mollis, namely M47, M51, and M42, were subjected to chromosomal characterization via cytogenetic identification, the analysis of molecular markers, and genomic in situ hybridization. These derivatives were all morphologically and cytogenetically stable. M47 was highly resistant to powdery mildew and nearly immune to stripe rust at the adult stage, and the chromosome constitution of this derivative can be expressed as 2n = 56 = 42T.a + 14L.m (where T.a = T. aestivum chromosomes; L.m = L. mollis chromosomes). Compared to M47, M42 was also resistant to stripe rust but was susceptible to powdery mildew; the chromosome constitution of M42 was 2n = 54 = 42T.a + 12L.m, in which a pair of homoeologous group 7 L.m chromosomes was eliminated. Finally, M51 was susceptible to powdery mildew and stripe rust and had a chromosome constitution of 2n = 48 = 42T.a + 6L.m, in which four pairs of L.m chromosomes from homoeologous groups 2, 4, 5, and 7 were eliminated. The differing disease resistances of the three derivatives are discussed in this report in the context of their chromosomal variations; this information can thus contribute to breeding disease resistant wheat with the potential for applying these derivatives as useful bridge materials.

scholarly journals Molecular cytogenetics and St-chromosome-specific molecular markers development of novel stripe rust resistant wheat–Thinopyrum intermedium as well as wheat–Thinopyrum ponticum substitution lines

2021 ◽  
Author(s):  
Siwen Wang ◽  
Changyou Wang ◽  
Xianbo Feng ◽  
Jixin Zhao ◽  
Pingchuan Deng ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Stripe Rust ◽  
Rust Resistance ◽  
Snp Array ◽  
Kernel Weight ◽  
Stripe Rust Resistance ◽  
Substitution Lines ◽  
Fish Genome ◽  
Thinopyrum Ponticum

Abstract Background Owing to the excellent resistance to abiotic and biotic stress, Thionpyrum intermedium (2n = 6x = 42, JJJsJsStSt) and Thinopyrum ponticum (2n = 10x = 70) are both widely utilized in wheat germplasm innovation programs. Disomic substitution lines (DSLs) carrying one pair of alien chromosomes are valuable bridge materials for novel genes transmission, FISH karyotype construction and specific molecular marker development. Results Six wheat–Thinopyrum DSLs derived from crosses between Abbondanza nullisomic lines (2n = 40) and two octoploid Trititrigia lines (2n = 8x = 56), were characterized by a sequential fluorescence in situ hybridization (FISH)–genome in situ hybridization (GISH), a multicolor GISH (mc-GISH), and an analysis of wheat 15K SNP array combined with molecular marker selection. ES-9 (DS2St (2A)) and ES-10 (DS3St (3D)) are wheat–Th. ponticum DSLs, while ES-23 (DS2St (2A)), ES-24 (DS3St (3D)), ES-25(DS2St (2B)), and ES-26 (DS2St (2D)) are wheat–Th. intermedium DSLs. ES-9, ES-23, ES-25 and ES-26 conferred higher thousand-kernel weight and stripe rust resistance at adult stages, while ES-10 and ES-24 performed highly resistant to stripe rust at all stages. Furthermore, cytological analysis showed that the alien chromosomes (2St/3St) belonging to the same homoeologous group derived from different donors carried the same FISH karyotype and could normally form a bivalent. Based on specific-locus amplified fragment sequencing (SLAF-seq), two 2St-chromosome-specific markers (PTH-005 and PTH-013) and two 3St-chromosome-specific markers (PTH-113 and PTH-135) were developed. Conclusions The six wheat–Thinopyrum disomic substitution lines conferring stripe rust resistance will be used as bridging parents for valuable resistant genes transmission. And the utility of PTH-113 and PTH-135 in a BC1F2 population showed the newly developed markers could be useful tools for efficient identification of St chromosomes in a common wheat background.

Sign in / Sign up

Export Citation Format

Share Document