scholarly journals Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum–Secale–Thinopyrum trigeneric hybrids

Genome ◽  
2017 ◽  
Vol 60 (8) ◽  
pp. 687-694 ◽  
Author(s):  
Yi Dai ◽  
Yamei Duan ◽  
Dawn Chi ◽  
Huiping Liu ◽  
Shuai Huang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Chromosome Identification ◽  
Distant Hybridization ◽  
Wheat Improvement ◽  
Thinopyrum Elongatum ◽  
Trigeneric Hybrids

It is very important to use chromosome-specific markers for identifying alien chromosomes in advanced generations of distant hybridization. The chromosome-specific markers of rye and Thinopyrum elongatum, as well as genomic in situ hybridization, were used to identify the alien chromosomes in eight lines that were derived from the crossing between Triticum trititrigia (AABBEE) and triticale (AABBRR). The results showed that four lines contained all rye chromosomes but no Th. elongatum chromosomes. The line RE36-1 contained all of the rye chromosomes except for chromosome 2R. The lines RE33-2 and RE62-1 contained all rye chromosomes and 1E and 5E translocated chromosome, respectively. The line RE24-4 contained 12 rye chromosomes plus a 7E chromosome or 12 rye chromosomes plus one R–E translocated chromosome. Chromosome identification in the above lines was consistent using chromosome-specific markers and genomic in situ hybridization. These chromosome-specific markers provide useful tools for detecting alien chromosomes in trigeneric hybrids, and these lines could be utilized as valuable germplasm in wheat improvement.

Synthesis and cytological characterization of trigeneric hybrids of durum wheat with and without Ph1

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1173-1181 ◽  
Author(s):  
Prem P Jauhar ◽  
M Doğramaci ◽  
T S Peterson
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Pairing ◽  
Genetic Recombination ◽  
Genomic In Situ Hybridization ◽  
Homoeologous Pairing ◽  
Alien Gene Transfer ◽  
Chromosome 5B ◽  
Alien Gene ◽  
Trigeneric Hybrids

Wild grasses in the tribe Triticeae, some in the primary or secondary gene pool of wheat, are excellent reservoirs of genes for superior agronomic traits, including resistance to various diseases. Thus, the diploid wheatgrasses Thinopyrum bessarabicum (Savul. and Rayss) Á. Löve (2n = 2x = 14; JJ genome) and Lophopyrum elongatum (Host) Á. Löve (2n = 2x = 14; EE genome) are important sources of genes for disease resistance, e.g., Fusarium head blight resistance that may be transferred to wheat. By crossing fertile amphidiploids (2n = 4x = 28; JJEE) developed from F1 hybrids of the 2 diploid species with appropriate genetic stocks of durum wheat, we synthesized trigeneric hybrids (2n = 4x = 28; ABJE) incorporating both the J and E genomes of the grass species with the durum genomes A and B. Trigeneric hybrids with and without the homoeologous-pairing suppressor gene, Ph1, were produced. In the absence of Ph1, the chances of genetic recombination between chromosomes of the 2 useful grass genomes (JE) and those of the durum genomes (AB) would be enhanced. Meiotic chromosome pairing was studied using both conventional staining and fluorescent genomic in situ hybridization (fl-GISH). As expected, the Ph1-intergeneric hybrids showed low chromosome pairing (23.86% of the complement), whereas the trigenerics with ph1b (49.49%) and those with their chromosome 5B replaced by 5D (49.09%) showed much higher pairing. The absence of Ph1 allowed pairing and, hence, genetic recombination between homoeologous chromosomes. Fl-GISH analysis afforded an excellent tool for studying the specificity of chromosome pairing: wheat with grass, wheat with wheat, or grass with grass. In the trigeneric hybrids that lacked chromosome 5B, and hence lacked the Ph1 gene, the wheat–grass pairing was elevated, i.e., 2.6 chiasmata per cell, a welcome feature from the breeding standpoint. Using Langdon 5D(5B) disomic substitution for making trigeneric hybrids should promote homoeologous pairing between durum and grass chromosomes and hence accelerate alien gene transfer into the durum genomes.Key words: alien gene transfer, chiasma (xma) frequency, chromosome pairing, fluorescent genomic in situ hybridization (fl-GISH), homoeologous-pairing regulator, specificity of chromosome pairing, wheatgrass.

Molecular cytogenetic analysis of wheat–barley hybrids using genomic in situ hybridization and barley microsatellite markers

Genome ◽  
2003 ◽  
Vol 46 (2) ◽  
pp. 314-322 ◽  
Author(s):  
L Malysheva ◽  
T Sjakste ◽  
F Matzk ◽  
M Röder ◽  
M Ganal
Keyword(s):  
Molecular Markers ◽  
In Situ Hybridization ◽  
Microsatellite Markers ◽  
Genetic Material ◽  
Introgressive Hybridization ◽  
Intergeneric Hybrids ◽  
Genomic In Situ Hybridization ◽  
Genome Constitution ◽  
Hybrid Plants

In the present investigation, genomic in situ hybridization (GISH) and barley microsatellite markers were used to analyse the genome constitution of wheat–barley hybrids from two backcross generations (BC1 and BC2). Two BC1 plants carried 3 and 6 barley chromosomes, respectively, according to GISH data. Additional chromosomal fragments were detected using microsatellites. Five BC2 plants possessed complete barley chromosomes or chromosome segments and six BC2 plants did not preserve barley genetic material. Molecular markers revealed segments of the barley genome with the size of one marker only, which probably resulted from recombination between wheat and barley chromosomes. The screening of backcrossed populations from intergeneric hybrids could be effectively conducted using both genomic in situ hybridization and molecular microsatellite markers. GISH images presented a general overview of the genome constitution of the hybrid plants, while microsatellite analysis revealed the genetic identity of the alien chromosomes and chromosomal segments introgressed. These methods were complementary and provided comprehensive information about the genomic constitution of the plants produced.Key words: wheat–barley hybrids, introgressive hybridization, recombination, molecular markers, genomic in situ hybridization (GISH).

CHROMOSOME IDENTIFICATION IN THE GENUS LILIUM USING COMPARATIVE GENOMIC IN SITU HYBRIDIZATION (CGISH)

2010 ◽  
pp. 35-40
Author(s):  
R. Barba-Gonzalez ◽  
M. de los Milagros Revuelta-Arreola ◽  
A.G. Rodriguez-Rodriguez ◽  
F. Santacruz-Ruvalcaba
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Comparative Genomic ◽  
Chromosome Identification

scholarly journals Chromosome Identification and Karyotyping of Satsuma Mandarin by Genomic In Situ Hybridization

2007 ◽  
Vol 132 (6) ◽  
pp. 836-841 ◽  
Author(s):  
Akira Kitajima ◽  
Atsu Yamasaki ◽  
Tsuyoshi Habu ◽  
Bannarat Preedasuttijit ◽  
Kojiro Hasegawa
Keyword(s):  
In Situ Hybridization ◽  
Relative Size ◽  
Relative Length ◽  
Fluorescein Isothiocyanate ◽  
Genomic In Situ Hybridization ◽  
Citrus Unshiu ◽  
Chromosome Identification ◽  
Satsuma Mandarin ◽  
Type C

Satsuma mandarin (Citrus unshiu Marcow.) chromosomes were stained with Giemsa and fluorochromes chromomycin A3 (CMA)/4′,6-diamidino-2-phenyindole (DAPI). Eighteen chromosomes were categorized into eight groups by the position and relative size of the CMA (+) region and relative length of chromosome. Ponkan (C. reticulata Blanco) DNA labeled with Dig-rhodamine (red) and pummelo [C. maxima (Burm.) Merr.] DNA labeled with biotin-fluorescein isothiocyanate (green) were used as genomic in situ hybridization (GISH) probes. GISH signals were detected on CMA (+) regions and other heterochromatin blocks. The chromosomes were categorized into 12 groups by the coloration and size of GISH signals with relative length of chromosomes. GISH allowed six pairs of speculated homozygous and six individual heterozygous chromosomes of satsuma mandarin to be identified unambiguously. In 10 chromosomes with distinct GISH signals on the CMA (+) regions, red GISH signals were detected on nine chromosomes, indicating that satsuma mandarin is closely related to ponkan. Two colors (red and green) of GISH signals were detected on type C chromosome and three different colors (red, green, and yellow) were detected on type A, indicating that pummelo is involved in the origin of satsuma mandarin. The origins of types A and C chromosomes in satsuma mandarin were also discussed. This article demonstrates that GISH is a powerful tool for chromosome identification and karyotyping in citrus.

Genome constitutions ofRoegneriaalashanica,R.elytrigioides,R.magnicaespesandR.grandis(Poaceae: Triticeae) via genomic in-situ hybridization

2010 ◽  
Vol 28 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Hai-Qing Yu ◽  
Chun Zhang ◽  
Chun-Bang Ding ◽  
Hai-Qin Zhang ◽  
Yong-Hong Zhou
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization
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