Molecular cytogenetic analysis of wheat–barley hybrids using genomic in situ hybridization and barley microsatellite markers

Genome ◽  
2003 ◽  
Vol 46 (2) ◽  
pp. 314-322 ◽  
Author(s):  
L Malysheva ◽  
T Sjakste ◽  
F Matzk ◽  
M Röder ◽  
M Ganal
Keyword(s):  
Molecular Markers ◽  
In Situ Hybridization ◽  
Microsatellite Markers ◽  
Genetic Material ◽  
Introgressive Hybridization ◽  
Intergeneric Hybrids ◽  
Genomic In Situ Hybridization ◽  
Genome Constitution ◽  
Hybrid Plants

In the present investigation, genomic in situ hybridization (GISH) and barley microsatellite markers were used to analyse the genome constitution of wheat–barley hybrids from two backcross generations (BC1 and BC2). Two BC1 plants carried 3 and 6 barley chromosomes, respectively, according to GISH data. Additional chromosomal fragments were detected using microsatellites. Five BC2 plants possessed complete barley chromosomes or chromosome segments and six BC2 plants did not preserve barley genetic material. Molecular markers revealed segments of the barley genome with the size of one marker only, which probably resulted from recombination between wheat and barley chromosomes. The screening of backcrossed populations from intergeneric hybrids could be effectively conducted using both genomic in situ hybridization and molecular microsatellite markers. GISH images presented a general overview of the genome constitution of the hybrid plants, while microsatellite analysis revealed the genetic identity of the alien chromosomes and chromosomal segments introgressed. These methods were complementary and provided comprehensive information about the genomic constitution of the plants produced.Key words: wheat–barley hybrids, introgressive hybridization, recombination, molecular markers, genomic in situ hybridization (GISH).

Genome discrimination by in situ hybridization in Icelandic species of Elymus and Elytrigia (Poaceae: Triticeae)

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Marian Ørgaard ◽  
Kesara Anamthawat-Jónsson
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Genomic Dna ◽  
Genomic In Situ Hybridization ◽  
Pseudoroegneria Spicata ◽  
Genome Constitution ◽  
Elytrigia Repens ◽  
Elymus Caninus ◽  
Elymus Species

The genome constitution of Icelandic Elymus caninus, E. alaskanus, and Elytrigia repens was examined by fluorescence in situ hybridization using genomic DNA and selected cloned sequences as probes. Genomic in situ hybridization (GISH) of Hordeum brachyantherum ssp. californicum (diploid, H genome) probe confirmed the presence of an H genome in the two tetraploid Elymus species and identified its presence in the hexaploid Elytrigia repens. The H chromosomes were painted uniformly except for some chromosomes of Elytrigia repens which showed extended unlabelled pericentromeric and subterminal regions. A mixture of genomic DNA from H. marinum ssp. marinum (diploid,Xa genome) and H. murinum ssp. leporinum (tetraploid,Xu genome) did not hybridize to chromosomes of the Elymus species or Elytrigia repens, confirming that these genomes were different from the H genome. The St genomic probe from Pseudoroegneria spicata (diploid) did not discriminate between the genomes of the Elymus species, whereas it produced dispersed and spotty hybridization signals most likely on the two St genomes of Elytrigia repens. Chromosomes of the two genera Elymus and Elytrigia showed different patterns of hybridization with clones pTa71 and pAes41, while clones pTa1 and pSc119.2 hybridized only to Elytrigia chromosomes. Based on FISH with these genomic and cloned probes, the two Elymus species are genomically similar, but they are evidently different from Elytrigia repens. Therefore the genomes of Icelandic Elymus caninus and E. alaskanus remain as StH, whereas the genomes of Elytrigia repens are proposed as XXH.Key words: Elymus, Elytrigia, H genome, St genome, in situ hybridization.

Meiotic chromosome pairing in intergeneric hybrids of colchicaceous ornamentals revealed by genomic in situ hybridization (GISH)

Euphytica ◽  
2014 ◽  
Vol 200 (2) ◽  
pp. 251-257 ◽  
Author(s):  
Tomonari Kishimoto ◽  
Miki Yamakawa ◽  
Daisuke Nakazawa ◽  
Junji Amano ◽  
Sachiko Kuwayama ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Pairing ◽  
Meiotic Chromosome ◽  
Intergeneric Hybrids ◽  
Genomic In Situ Hybridization ◽  
Meiotic Chromosome Pairing

Genomic in situ hybridization in plants with small genomes is feasible and elucidates the chromosomal parentage in interspecific Arabidopsis hybrids

Genome ◽  
2004 ◽  
Vol 47 (5) ◽  
pp. 954-960 ◽  
Author(s):  
Hoda B.M Ali ◽  
Martin A Lysak ◽  
Ingo Schubert
Keyword(s):  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Closely Related Species ◽  
Meiotic Chromosomes ◽  
Hybrid Plants ◽  
Synthetic Hybrids ◽  
High Concentrations ◽  
Arabidopsis Suecica ◽  
Allotetraploid Species

Genomic in situ hybridization (GISH) is a useful tool to analyse natural polyploids, hybrid plants, and their backcross progenies as to their origin, genomic composition, and intergenomic rearrangements. However, in angiosperms with very small genomes (<0.6 pg/1 C), often only heterochromatic regions were found to be labeled. We have modified the GISH technique to label entire mitotic and meiotic chromosomes of Arabidopsis thaliana (2n = 10) and closely related species with very small genomes by using high concentrations of DNA (7.5–15 µg per probe per slide) or 5 µg of probe and long hybridization times (>60 h). According to our GISH data, Cardaminopsis carpatica (2n = 16) is most likely the diploid ancestor of the autotetraploid Arabidopsis arenosa (2n = 32). Furthermore, within the allotetraploid species Arabidopsis suecica (2n = 26), it was possible to elucidate the origin of chromosomes contributed by the parental species A. thaliana and A. arenosa for a specimen with 2n = 26 or a deviating chromosome number.Key words: genomic in situ hybridization (GISH), Arabidopsis, Brassicaceae, allopolyploids, synthetic hybrids.

scholarly journals Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum–Secale–Thinopyrum trigeneric hybrids

Genome ◽  
2017 ◽  
Vol 60 (8) ◽  
pp. 687-694 ◽  
Author(s):  
Yi Dai ◽  
Yamei Duan ◽  
Dawn Chi ◽  
Huiping Liu ◽  
Shuai Huang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Chromosome Identification ◽  
Distant Hybridization ◽  
Wheat Improvement ◽  
Thinopyrum Elongatum ◽  
Trigeneric Hybrids

It is very important to use chromosome-specific markers for identifying alien chromosomes in advanced generations of distant hybridization. The chromosome-specific markers of rye and Thinopyrum elongatum, as well as genomic in situ hybridization, were used to identify the alien chromosomes in eight lines that were derived from the crossing between Triticum trititrigia (AABBEE) and triticale (AABBRR). The results showed that four lines contained all rye chromosomes but no Th. elongatum chromosomes. The line RE36-1 contained all of the rye chromosomes except for chromosome 2R. The lines RE33-2 and RE62-1 contained all rye chromosomes and 1E and 5E translocated chromosome, respectively. The line RE24-4 contained 12 rye chromosomes plus a 7E chromosome or 12 rye chromosomes plus one R–E translocated chromosome. Chromosome identification in the above lines was consistent using chromosome-specific markers and genomic in situ hybridization. These chromosome-specific markers provide useful tools for detecting alien chromosomes in trigeneric hybrids, and these lines could be utilized as valuable germplasm in wheat improvement.

Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae

Euphytica ◽  
2011 ◽  
Vol 181 (2) ◽  
pp. 197-202 ◽  
Author(s):  
Daisuke Nakazawa ◽  
Tomonari Kishimoto ◽  
Takeru Sato ◽  
Tomoka Saito ◽  
Junji Amano ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Intergeneric Hybrids ◽  
Genomic In Situ Hybridization ◽  
Gish Analysis

Introgression of Perennial Teosinte Genome into Maize and Identification of Genomic In Situ Hybridization and Microsatellite Markers

Crop Science ◽  
2005 ◽  
Vol 45 (2) ◽  
pp. 717-721 ◽  
Author(s):  
Qilin Tang ◽  
Tingzhao Rong ◽  
Yunchun Song ◽  
Junpin Yang ◽  
Guangtang Pan ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Microsatellite Markers ◽  
Genomic In Situ Hybridization
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