Chromosomal location of AFLP markers in common wheat utilizing nulli-tetrasomic stocks

Genome ◽  
2000 ◽  
Vol 43 (2) ◽  
pp. 298-305 ◽  
Author(s):  
Xiuqiang Huang ◽  
Friedrich J Zeller ◽  
Sai LK Hsam ◽  
Gerhard Wenzel ◽  
Volker Mohler
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Fragment Length Polymorphism ◽  
Chromosomal Location ◽  
Wheat Cultivar ◽  
Aflp Markers ◽  
Common Wheat Cultivar ◽  
Chromosome Assignment ◽  
Aneuploid Chromosome ◽  
The Common

Amplified fragment length polymorphism (AFLP) markers with a total of 256 EcoRI + ANN- MseI + CNN primer combinations were investigated employing the common wheat cultivar Triticum aestivum 'Chinese Spring.' On average, 103 fragments per primer combination were amplified, ranging from a maximum of 226 fragments to a minimum of 18 fragments. The primer combinations E + AAA - M + CNN and E + ATT - M + CNN produced very few distinct fragments. By using 15 randomly chosen EcoRI + ANN - MseI + CNN primer combinations, 928 AFLP markers were allocated to wheat chromosomes, of which 131 were assigned to specific chromosome arms. These AFLP markers were locus-specific and randomly distributed on the different chromosomes. In addition, 6 and 41 AFLP markers were simultaneously absent in two nulli-tetrasomics (NTs) of both homoeologous and non-homoeologous groups, respectively, whereas additional fragments were detected in N1BT1A, N5AT5D, and N6BT6A lines.Key words: aneuploid, chromosome assignment, Triticum aestivum.

Identification of a gene for resistance to wheatgrass powdery mildew fungus in the common wheat cultivar Chinese Spring

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 612-614 ◽  
Author(s):  
Y. Tosa ◽  
H. Tokunaga ◽  
H. Ogura
Keyword(s):  
Triticum Aestivum ◽  
Powdery Mildew ◽  
Common Wheat ◽  
Chinese Spring ◽  
Wheat Cultivar ◽  
Erysiphe Graminis ◽  
Common Wheat Cultivar ◽  
Powdery Mildew Fungus ◽  
Hybrid Culture ◽  
The Common

A gene for resistance to Erysiphe graminis was detected in Triticum aestivum cv. Chinese Spring, strain Salmon, T. compactum cv. No. 44, and T. spelta var. duhamelianum, using a hybrid culture derived from E. graminis f. sp. agropyri × E. graminis f. sp. tritici. The gene was located on the short arm of chromosome 6B and designated Pm11. Pm11 was considered to be involved in the resistance of wheat to the wheatgrass powdery mildew fungus.Key words: wheat, resistance, powdery mildew, Erysiphe graminis.

A cytogenetic study of the blue-grain line of the common wheat cultivar Saratovskaya 29

2012 ◽  
Vol 48 (8) ◽  
pp. 785-791 ◽  
Author(s):  
V. S. Arbuzova ◽  
E. D. Badaeva ◽  
T. T. Efremova ◽  
T. S. Osadchaya ◽  
N. V. Trubacheeva ◽  
...  
Keyword(s):  
Common Wheat ◽  
Wheat Cultivar ◽  
Cytogenetic Study ◽  
Common Wheat Cultivar ◽  
The Common

scholarly journals Mutants at gliadin loci on the basis of the common wheat cultivar Bezostaya 1

2019 ◽  
Vol 24 ◽  
pp. 109-114 ◽  
Author(s):  
N. A. Kozub ◽  
I. A. Sozinov ◽  
H. Ya. Bidnyk ◽  
N. A. Demianova ◽  
O. I. Sozinova ◽  
...  
Keyword(s):  
Common Wheat ◽  
Storage Proteins ◽  
Wheat Cultivar ◽  
Polyacrylamide Gel Electrophoresis ◽  
Induced Mutations ◽  
Common Wheat Cultivar ◽  
The Common ◽  
First Time ◽  
Sds Electrophoresis

Aim. The aim of this study was to isolate and propagate mutants at gliadin loci developed on the basis of the common wheat cultivar Bezostaya 1. Methods. We searched for spontaneous and gamma-irradiation induced mutations at gliadin loci among the progeny of F1 and F2 plants from crosses between near-isogenic lines by gliadin loci on the basis of the cultivar Bezostaya 1, including lines with the wheat-rye 1BL.1RS translocation. To identify mutations, we performed acid polyacrylamide gel electrophoresis and SDS-electrophoresis of storage proteins. Results. On the basis of the common wheat cultivar Bezostaya 1, five mutants (six mutations) at gliadin loci were isolated and propagated, four of which were described for the first time. Three mutations occurred at the Gli-R1 locus involved in the wheat-rye 1BL.1RS translocation (the loss of secalins, intensification of a secalin component, and increased mobility of a secalin component). Two mutations were identified in the allele Gli-B1b, one caused the null-allele at the Gli-A2 locus. Conclusions. The material of mutants is of importance for studying the role of certain groups of storage proteins and their components in quality determination, as well as mechanisms of regulation of storage protein synthesis. Keywords: Triticum aestivum, gliadin, secalin, mutation, 1BL.1RS translocation.

Operation of resistance genes in wheat to Erysiphe graminis f.sp. tritici against E. graminis f.sp. agropyri

Genome ◽  
1990 ◽  
Vol 33 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Y. Tosa ◽  
S. Tada
Keyword(s):  
Powdery Mildew ◽  
Common Wheat ◽  
Resistance Genes ◽  
Wheat Cultivar ◽  
Erysiphe Graminis ◽  
Near Isogenic Lines ◽  
Avirulence Genes ◽  
Common Wheat Cultivar ◽  
Isogenic Lines ◽  
The Common

The common wheat cultivar 'Chancellor' and its near-isogenic lines carrying resistance genes to Erysiphe graminis f.sp. tritici were inoculated with 240 F1 cultures derived from a cross between E. graminis f.sp. agropyri, Ak-1, and E. graminis f.sp. tritici, Tk-1. Segregation patterns of avirulent and virulent cultures suggested that the F1 population carries avirulence genes corresponding to Pm1, Pm2, Pm3a, and Pm3b. These avirulence genes were considered to be derived from Ak-1. It was therefore concluded that Pm1, Pm2, Pm3a, and Pm3b, resistance genes to E. graminis f.sp. tritici, operate against E. graminis f.sp. agropyri.Key words: powdery mildew, Erysiphe graminis, resistance, wheat, wheatgrass.

The effect of different Agropyron elongatum chromosomes on pairing in Agropyron – common wheat hybrids

Genome ◽  
1988 ◽  
Vol 30 (6) ◽  
pp. 978-983 ◽  
Author(s):  
A. Charpentier ◽  
M. Feldman ◽  
Y. Cauderon
Keyword(s):  
Common Wheat ◽  
Chromosome Pairing ◽  
Chinese Spring ◽  
Chromosomal Location ◽  
Wheat Cultivar ◽  
Agropyron Elongatum ◽  
The Common ◽  
Disomic Addition Lines ◽  
Wheat Hybrids ◽  
Tetraploid Cytotypes

Chromosome pairing at first meiotic metaphase was studied in hybrids between the common wheat cultivar Chinese Spring (CS) and an induced autotetraploid line derived from diploid Agropyron elongatum. The latter was found to carry genes for homoeologous pairing. To determine the chromosomal location of these and other genes that control pairing, disomic addition lines of A. elongatum in the cv. Chinese Spring were crossed with tetraploid cytotypes of A. elongatum, and pairing was then compared in the resulting hybrids and in hybrids between cv. Chinese Spring and tetraploid A. elongatum. The elongatum chromosomes were classified into those that suppress (6E), promote (5E, 3E, and possibly 1E), or have no effect on pairing (4E). The effect of chromosomes 2E and 7E was not studied. Chromosomes 5E and 3E differed in their effect on the degree and pattern of chromosome pairing. These findings are compared with the available data on the control of pairing in A. elongatum and in other Triticinae species.Key words: chromosome pairing, pairing promoter, Triticum, common wheat, Agropyron.

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