Arrays of repetitive DNA elements in the largest chromosomes of Toxoplasma gondii

Genome ◽  
1999 ◽  
Vol 42 (2) ◽  
pp. 265-269 ◽  
Author(s):  
Mariana Matrajt ◽  
Sergio O Angel ◽  
Viviana Pszenny ◽  
Eduardo Guarnera ◽  
David S Roos ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Repetitive Dna ◽  
Pulse Field ◽  
Pulse Field Gel Electrophoresis ◽  
Molecular Karyotype ◽  
Tandem Structure ◽  
Dna Elements ◽  
Repeated Structure ◽  
Lambda Phages ◽  
Repetitive Dna Elements

A novel tandemly repeated DNA structure of Toxoplasma gondii that meets the requirements assigned for satellital DNA was characterized. A DNA fragment of 1002 bp contains two different elements of repetitive DNA families named ABGTg7 and ABGTg8.2. Both repeats are members of a more complex tandem structure where ABGTg7-like monomers can be arranged either as direct tandems or flanked by other related or non-related repeats. Pulse-field gel electrophoresis analysis showed that these repeats hybridize with the largest T. gondii chromosomes. Bal31 sensitivity assays indicated that these elements are located near the telomeres and along other regions too. Five genomic lambda phages were isolated and two different completed clusters of the repeated structure were analyzed.Key words: Toxoplasma gondii, tandem repeat, satellite DNA, molecular karyotype, telomere.

Arrays of repetitive DNA elements in the largest chromosomes of Toxoplasma gondii

Genome ◽  
1999 ◽  
Vol 42 (2) ◽  
pp. 265-269 ◽  
Author(s):  
Mariana Matrajt ◽  
Sergio O. Angel ◽  
Viviana Pszenny ◽  
Eduardo Guarnera ◽  
David S. Roos ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Repetitive Dna ◽  
Dna Elements ◽  
Repetitive Dna Elements

Sequence Organization of Simple, Highly Repetitive DNA Elements inBrassicaspecies

1991 ◽  
Vol 42 (2) ◽  
pp. 243-249 ◽  
Author(s):  
D. R SIBSON ◽  
S. G. HUGHES ◽  
J. A. BRYANT ◽  
P. N. FITCHETT
Keyword(s):  
Repetitive Dna ◽  
Sequence Organization ◽  
Dna Elements ◽  
Highly Repetitive Dna ◽  
Repetitive Dna Elements

A rapid procedure for the isolation of C0t-1 DNA from plants

Genome ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 138-142 ◽  
Author(s):  
Michael S. Zwick ◽  
Robert E. Hanson ◽  
M. Nurul Islam-Faridi ◽  
David M. Stelly ◽  
Rod A. Wing ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Repetitive Dna ◽  
Copy Number ◽  
Genomic Dna ◽  
Mammalian Species ◽  
Repetitive Dnas ◽  
Rapid Procedure ◽  
Dna Elements ◽  
Repetitive Dna Elements

In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or "blocking" of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.Key words: C0t-1 DNA, in situ hybridization, BACs, plants.

scholarly journals Use of repetitive DNA elements to define genetic relationships amongAnaplasma marginaleisolates

2001 ◽  
Vol 197 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Alda Maria T. Ferreira ◽  
Sérgio Suzart ◽  
Odilon Vidotto ◽  
Don P. Knowles ◽  
Marilda C. Vidotto
Keyword(s):  
Repetitive Dna ◽  
Genetic Relationships ◽  
Dna Elements ◽  
Repetitive Dna Elements

Methylation dynamics of repetitive DNA elements in the mouse germ cell lineage

Genomics ◽  
2003 ◽  
Vol 82 (2) ◽  
pp. 230-237 ◽  
Author(s):  
D.J. Lees-Murdock ◽  
M. De Felici ◽  
C.P. Walsh
Keyword(s):  
Germ Cell ◽  
Repetitive Dna ◽  
Cell Lineage ◽  
Dna Elements ◽  
Repetitive Dna Elements
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