scholarly journals A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements

2004 ◽  
Vol 32 (3) ◽  
pp. 38e-38 ◽  
Author(s):  
A. S. Yang
Keyword(s):  
Dna Methylation ◽  
Repetitive Dna ◽  
Global Dna Methylation ◽  
Simple Method ◽  
Dna Elements ◽  
Repetitive Dna Elements

DNA methylation in satellite repeats disorders

2019 ◽  
Vol 63 (6) ◽  
pp. 757-771 ◽  
Author(s):  
Claire Francastel ◽  
Frédérique Magdinier
Keyword(s):  
Dna Methylation ◽  
Human Genome ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Satellite Repeats ◽  
Tremendous Progress ◽  
Genes Encoding ◽  
Dna Elements ◽  
Near Future

Abstract Despite the tremendous progress made in recent years in assembling the human genome, tandemly repeated DNA elements remain poorly characterized. These sequences account for the vast majority of methylated sites in the human genome and their methylated state is necessary for this repetitive DNA to function properly and to maintain genome integrity. Furthermore, recent advances highlight the emerging role of these sequences in regulating the functions of the human genome and its variability during evolution, among individuals, or in disease susceptibility. In addition, a number of inherited rare diseases are directly linked to the alteration of some of these repetitive DNA sequences, either through changes in the organization or size of the tandem repeat arrays or through mutations in genes encoding chromatin modifiers involved in the epigenetic regulation of these elements. Although largely overlooked so far in the functional annotation of the human genome, satellite elements play key roles in its architectural and topological organization. This includes functions as boundary elements delimitating functional domains or assembly of repressive nuclear compartments, with local or distal impact on gene expression. Thus, the consideration of satellite repeats organization and their associated epigenetic landmarks, including DNA methylation (DNAme), will become unavoidable in the near future to fully decipher human phenotypes and associated diseases.

Sequence Organization of Simple, Highly Repetitive DNA Elements inBrassicaspecies

1991 ◽  
Vol 42 (2) ◽  
pp. 243-249 ◽  
Author(s):  
D. R SIBSON ◽  
S. G. HUGHES ◽  
J. A. BRYANT ◽  
P. N. FITCHETT
Keyword(s):  
Repetitive Dna ◽  
Sequence Organization ◽  
Dna Elements ◽  
Highly Repetitive Dna ◽  
Repetitive Dna Elements

A rapid procedure for the isolation of C0t-1 DNA from plants

Genome ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 138-142 ◽  
Author(s):  
Michael S. Zwick ◽  
Robert E. Hanson ◽  
M. Nurul Islam-Faridi ◽  
David M. Stelly ◽  
Rod A. Wing ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Repetitive Dna ◽  
Copy Number ◽  
Genomic Dna ◽  
Mammalian Species ◽  
Repetitive Dnas ◽  
Rapid Procedure ◽  
Dna Elements ◽  
Repetitive Dna Elements

In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or "blocking" of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.Key words: C0t-1 DNA, in situ hybridization, BACs, plants.

scholarly journals Use of repetitive DNA elements to define genetic relationships amongAnaplasma marginaleisolates

2001 ◽  
Vol 197 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Alda Maria T. Ferreira ◽  
Sérgio Suzart ◽  
Odilon Vidotto ◽  
Don P. Knowles ◽  
Marilda C. Vidotto
Keyword(s):  
Repetitive Dna ◽  
Genetic Relationships ◽  
Dna Elements ◽  
Repetitive Dna Elements

Methylation dynamics of repetitive DNA elements in the mouse germ cell lineage

Genomics ◽  
2003 ◽  
Vol 82 (2) ◽  
pp. 230-237 ◽  
Author(s):  
D.J. Lees-Murdock ◽  
M. De Felici ◽  
C.P. Walsh
Keyword(s):  
Germ Cell ◽  
Repetitive Dna ◽  
Cell Lineage ◽  
Dna Elements ◽  
Repetitive Dna Elements

Arrays of repetitive DNA elements in the largest chromosomes of Toxoplasma gondii

Genome ◽  
1999 ◽  
Vol 42 (2) ◽  
pp. 265-269 ◽  
Author(s):  
Mariana Matrajt ◽  
Sergio O. Angel ◽  
Viviana Pszenny ◽  
Eduardo Guarnera ◽  
David S. Roos ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Repetitive Dna ◽  
Dna Elements ◽  
Repetitive Dna Elements
Sign in / Sign up

Export Citation Format

Share Document