The development of RAPD and microsatellite markers in lodgepole pine (Pinus contorta var. latifolia)

Genome ◽  
1998 ◽  
Vol 41 (6) ◽  
pp. 797-805 ◽  
Author(s):  
Mark Hicks ◽  
Denise Adams ◽  
Sandra O'Keefe ◽  
Ellen Macdonald ◽  
Ross Hodgetts
Keyword(s):  
Dna Markers ◽  
Rocky Mountains ◽  
Microsatellite Loci ◽  
Genomic Dna ◽  
Pinus Contorta ◽  
Genomic Library ◽  
Lodgepole Pine ◽  
Ecological Studies ◽  
Polymorphic Microsatellite ◽  
Genome Analyses

Two types of polymorphic DNA markers suitable for genome analyses, population genetic and ecological studies on lodgepole pine (Pinus contorta var. latifolia) have been developed. We detected 52 decameric oligonucleotides, within a set of 200, that could be used to amplify the pine DNA. From these, 4 that allowed unambiguous amplification of 10 random polymorphic (RAPD) loci, have been chosen for use in this species. Five polymorphic microsatellite loci were found following the screening of a genomic library and all contained the AGn motif. Such loci were present at a frequency of 1 in every 345 kb pairs of genomic DNA. Three loci were comprised of a simple dinucleotide repeat, one locus was of the form TAn - GAm and one was ATn - AGm. The number of alleles at the 5 microsatellite loci ranged from 3 to 8 in a sample of 6 trees from the eastern foothills of the Rocky Mountains in Alberta and the alleles at all loci exhibited Mendelian segregation.Key words: DNA polymorphism, RAPD, microsatellite, SSR, Pinus contorta var. latifolia.

Highly polymorphic microsatellite markers in Chamaecyparis obtusa

2001 ◽  
Vol 31 (12) ◽  
pp. 2248-2251 ◽  
Author(s):  
Y Nakao ◽  
H Iwata ◽  
A Matsumoto ◽  
Y Tsumura ◽  
N Tomaru
Keyword(s):  
Microsatellite Loci ◽  
Magnetic Beads ◽  
Genomic Library ◽  
Gene Diversity ◽  
Pcr Amplification ◽  
Chamaecyparis Obtusa ◽  
Mendelian Inheritance ◽  
Average Gene Diversity ◽  
Polymorphic Microsatellite ◽  
Average Gene

Nine microsatellite loci in hinoki, Chamaecyparis obtusa (Sieb. et Zucc.) Endl., were identified and characterized. A genomic library, developed using enrichment with magnetic beads, was screened to identify microsatellite repeats (CT/AG). The microsatellite loci, where the alleles were segregated, displayed codominant Mendelian inheritance. Genetic analysis of 16 plus trees and two unrelated individuals of Chamaecyparis obtusa revealed that all loci were highly polymorphic, with an average of 10.3 alleles per locus, and an average gene diversity of 0.77. The applicability of these microsatellite loci was also tested in other species of the Cupressaceae and in Cryptomeria japonica (L.f.) D. Don (Taxodiaceae, a family closely related to Cupressaceae). Polymerase chain reaction (PCR) amplification was successful for about half of the loci of the species in the genus Chamaecyparis. However, the PCR amplification patterns of the 11 species of Cupressaceae showed no clear correlations with their molecular phylogeny. The highly polymorphic microsatellite loci in Chamaecyparis obtusa, identified here, will be useful in studies of hinoki breeding and population genetics.

Ten polymorphic microsatellite loci identified from a small insert genomic library forPeronospora tabacina

Mycologia ◽  
2012 ◽  
Vol 104 (3) ◽  
pp. 633-640 ◽  
Author(s):  
Robert N. Trigiano ◽  
Phillip A. Wadl ◽  
Deborah Dean ◽  
Denita Hadziabdic ◽  
Brian E. Scheffler ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Genomic Library ◽  
Polymorphic Microsatellite

scholarly journals Population genetics of the fish Brycon henni (Characiformes: Bryconidae) using species-specific polymorphic microsatellite loci

2020 ◽  
Vol 68 (3) ◽  
Author(s):  
Ricardo M. Landínez-García ◽  
Edna J. Márquez
Keyword(s):  
Population Genetics ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
International Union ◽  
Genetic Population ◽  
Genetic Diversity And Structure ◽  
Polymorphic Microsatellite ◽  
Species Specific ◽  
Low Coverage ◽  
Risk Categorization

Introduction: The freshwater fish Brycon henni (Characiformes: Bryconidae) is endemic to Colombia and currently considered as “least concern” according to the International Union for Conservation of Nature (IUCN). Objective: To develop microsatellite markers to examine population genetics in Brycon henni. Methods: Using a low-coverage sequenced genomic library, this study developed the first set of microsatellite loci to study the population genetics of this Neotropical species. These loci were used to evaluate the genetic diversity and structure of B. henni from three sites of the Magdalena-Cauca Basin (Colombia). Results: A set of twenty-one polymorphic microsatellite loci was highly informative and revealed that B. henni is evenly genetically structured between two tributaries of the Cauca River separated by only 30 km, a finding that indicates it conforms to reproductively isolated groups. Conclusions: We reported a set of twenty-one polymorphic microsatellite loci that was highly informative and allowed the detection of genetic structure at local and regional scales. This genetic population structure, concordant with that found in eight congeners, is relevant to estimate the B. henni risk categorization as well as for management, conservation, and restocking programs.

scholarly journals Isolation and Characterization of 10 Polymorphic Microsatellite Loci in Paphiopedilum concolor (Batem.) Pfitzer (Orchidaceae) and Cross-species Amplification

HortScience ◽  
2010 ◽  
Vol 45 (8) ◽  
pp. 1286-1287 ◽  
Author(s):  
Long-na Li ◽  
Songjun Zeng ◽  
Feng Zheng ◽  
Zhi-lin Chen ◽  
Kun-lin Wu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Microsatellite Locus ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
Enriched Genomic Library ◽  
Relationship Patterns ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite

Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.

Sign in / Sign up

Export Citation Format

Share Document