Three novel Nicotiana debneyi specific repetitive DNA elements derived from a RAPD marker

Genome ◽  
1999 ◽  
Vol 42 (1) ◽  
pp. 104-109 ◽  
Author(s):  
D Bai
Keyword(s):  
Repetitive Dna ◽  
Root Rot ◽  
Somatic Hybrids ◽  
Rapd Marker ◽  
Translocation Line ◽  
Black Root Rot ◽  
Dna Elements ◽  
Nicotiana Debneyi ◽  
Species Specific ◽  
Repetitive Dna Elements

The RAPD marker UBC4181050, tightly linked in coupling with the Nicotiana debneyi gene for resistance to black root rot (Chalara elegans Nag Raj and Kendrick; Syn. Thielaviopsis basicola [Berk. and Broome] Ferraris), has been cloned and sequenced. The terminal 10 bases of this RAPD marker exactly match the sequence of the primer UBC418. UBC4181050 was restricted into 6 subfragments of 75, 91, 110, 174, 274, and 336 bp (the accession numbers for these 6 subfragments in the GenBank Data Base are U84217, U84218, U84219, U84220, U84221, and U84222, respectively) by combined restriction with HindIII and RsaI. The entire UBC4181050 marker and its 6 subfragments were used as probes in RFLP analyses. The RFLP analyses were performed on 'Delgold' tobacco (Nicotiana tabacum L.), the tobacco plants (2n = 48) recovered from the somatic hybrids between 'Delgold' and N. debneyi, the 'Delgold' tobacco translocation line carrying the N. debneyi gene for resistance to black root rot, the 'Delgold' tobacco addition lines carrying various N. debneyi chromosomes, and an additional 27 species in the genus Nicotiana. The analyses indicated that UBC4181050 is composed of at least 6 different repetitive DNA elements that are independently interspersed in N. debneyi genomes. Three of them (subfragments of 91, 274, and 336 bp) are N. debneyi specific. The N. debneyi specific repetitive DNA elements are distributed primarily within the taxonomic section (Suaveolentes) to which N. debneyi belongs.Key words: RAPD, repetitive DNA, Nicotiana debneyi, species-specific.

Comparative Cytogenetics in Four Leptodactylus Species (Amphibia, Anura, Leptodactylidae): Evidence of Inner Chromosomal Diversification in Highly Conserved Karyotypes

2021 ◽  
pp. 1-11
Author(s):  
David S. da Silva ◽  
Heriberto F. da Silva Filho ◽  
Marcelo B. Cioffi ◽  
Edivaldo H.C. de Oliveira ◽  
Anderson J.B. Gomes
Keyword(s):  
18S Rdna ◽  
Repetitive Sequences ◽  
Molecular Cytogenetics ◽  
Comparative Cytogenetics ◽  
The Family ◽  
Agnor Staining ◽  
Dna Elements ◽  
Conventional Cytogenetics ◽  
Species Specific ◽  
Repetitive Dna Elements

With 82 species currently described, the genus <i>Leptodactylus</i> is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 <i>Leptodactylus</i> species (<i>L. macrosternum, L. pentadactylus, L. fuscus,</i> and <i>Leptodactylus</i> cf<i>. podicipinus</i>), combining conventional cytogenetics (Giemsa staining, C-banding, and AgNOR staining) and mapping of molecular markers (18S rDNA, telomeric and microsatellite probes), to investigate mechanisms underlying their karyotype differentiation process. The results showed that all species had karyotypes with 2n = 22 and FN = 44, except for <i>Leptodactylus</i> cf. <i>podicipinus</i> which presented FN = 36. The 18S rDNA was observed in pair 8 of all analyzed species (corresponding to pair 4 in <i>L. pentadactylus</i>), coinciding with the secondary constrictions and AgNOR staining. FISH with microsatellite DNA probes demonstrated species-specific patterns, as well as an association of these repetitive sequences with constitutive heterochromatin blocks and ribosomal DNA clusters, revealing the dynamics of microsatellites in the genome of the analyzed species. In summary, our data demonstrate an ongoing process of genomic divergence inside species with almost similar karyotype, driven most likely by a series of pericentric inversions, followed by differential accumulation of repetitive sequences.

Sequence Organization of Simple, Highly Repetitive DNA Elements inBrassicaspecies

1991 ◽  
Vol 42 (2) ◽  
pp. 243-249 ◽  
Author(s):  
D. R SIBSON ◽  
S. G. HUGHES ◽  
J. A. BRYANT ◽  
P. N. FITCHETT
Keyword(s):  
Repetitive Dna ◽  
Sequence Organization ◽  
Dna Elements ◽  
Highly Repetitive Dna ◽  
Repetitive Dna Elements

A rapid procedure for the isolation of C0t-1 DNA from plants

Genome ◽  
1997 ◽  
Vol 40 (1) ◽  
pp. 138-142 ◽  
Author(s):  
Michael S. Zwick ◽  
Robert E. Hanson ◽  
M. Nurul Islam-Faridi ◽  
David M. Stelly ◽  
Rod A. Wing ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Repetitive Dna ◽  
Copy Number ◽  
Genomic Dna ◽  
Mammalian Species ◽  
Repetitive Dnas ◽  
Rapid Procedure ◽  
Dna Elements ◽  
Repetitive Dna Elements

In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or "blocking" of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.Key words: C0t-1 DNA, in situ hybridization, BACs, plants.

scholarly journals Use of repetitive DNA elements to define genetic relationships amongAnaplasma marginaleisolates

2001 ◽  
Vol 197 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Alda Maria T. Ferreira ◽  
Sérgio Suzart ◽  
Odilon Vidotto ◽  
Don P. Knowles ◽  
Marilda C. Vidotto
Keyword(s):  
Repetitive Dna ◽  
Genetic Relationships ◽  
Dna Elements ◽  
Repetitive Dna Elements

Methylation dynamics of repetitive DNA elements in the mouse germ cell lineage

Genomics ◽  
2003 ◽  
Vol 82 (2) ◽  
pp. 230-237 ◽  
Author(s):  
D.J. Lees-Murdock ◽  
M. De Felici ◽  
C.P. Walsh
Keyword(s):  
Germ Cell ◽  
Repetitive Dna ◽  
Cell Lineage ◽  
Dna Elements ◽  
Repetitive Dna Elements

Arrays of repetitive DNA elements in the largest chromosomes of Toxoplasma gondii

Genome ◽  
1999 ◽  
Vol 42 (2) ◽  
pp. 265-269 ◽  
Author(s):  
Mariana Matrajt ◽  
Sergio O. Angel ◽  
Viviana Pszenny ◽  
Eduardo Guarnera ◽  
David S. Roos ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Repetitive Dna ◽  
Dna Elements ◽  
Repetitive Dna Elements
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