Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus

Genome ◽  
1998 ◽  
Vol 41 (1) ◽  
pp. 120-128 ◽  
Author(s):  
Sergei Svitashev ◽  
Tomas Bryngelsson ◽  
Xiaomei Li ◽  
Richard RC Wang
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Sequence Comparison ◽  
Rapd Markers ◽  
Homologous Sequence ◽  
Repetitive Dna Sequences ◽  
Comparison Analysis ◽  
Hordelymus Europaeus ◽  
Elymus Species ◽  
Specific Sequences

We have developed RFLP and RAPD markers specific for the genomes involved in the evolution of Elymus species, i.e., the St, Y, H, P, and W genomes. Two P genome specific repetitive DNA sequences, pAgc1 (350 bp) and pAgc30 (458 bp), and three W genome specific sequences, pAuv3 (221 bp), pAuv7 (200 bp), and pAuv13 (207 bp), were isolated from the genomes of Agropyron cristatum and Australopyrum velutinum, respectively. Attempts to find Y genome specific sequences were not successful. Primary-structure analysis demonstrated that pAgc1 (P genome) and pAgc30 (P genome) share 81% similarity over a 227-bp stretch. The three W genome specific sequences were also highly homologous. Sequence comparison analysis revealed no homology to sequences in the EMBL- GenBank databases. Three to four genome-specific RAPD markers were found for each of the five genomes. Genome-specific bands were cloned and demonstrated to be mainly low-copy sequences present in various Triticeae species. The RFLP and RAPD markers obtained, together with the previously described H and St genome specific clones pHch2 and pPlTaq2.5 and the Ns genome specific RAPD markers were used to investigate the genomic composition of a few Elymus species and Hordelymus europaeus, whose genome formulas were unknown. Our results demonstrate that only three of eight Elymus species examined (the tetraploid species Elymus grandis and the hexaploid speciesElymus caesifolius and Elymus borianus) really belong to Elymus.

A study of 28 Elymus species using repetitive DNA sequences

Genome ◽  
1996 ◽  
Vol 39 (6) ◽  
pp. 1093-1101 ◽  
Author(s):  
Sergei Svitashev ◽  
Björn Salomon ◽  
Tomas Bryngelsson ◽  
Roland von Bothmer
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Molecular Study ◽  
Repetitive Dna Sequences ◽  
Hybridization Pattern ◽  
Specific Hybridization ◽  
Elymus Species ◽  
Hordeum Species

Four repetitive DNA sequences cloned from the barley (Hordeum vulgare) genome and common for different Triticeae species were used for a molecular study of phylogenetic relationships among 28 Elymus species. Two wild Hordeum species (H genome), two Pseudoroegneria species (S genome), Agropyron cristatum (P genome), and Australopyrum velutinum (W genome) were included as genomic representatives for the genomes that supposedly were involved in the evolution of the genus Elymus. Our results are essentially congruent with the genomic classification system. This study demonstrates that Elymus is not a monophyletic genus. Based on an analysis of Southern blot hybridization we could discriminate between SY and SH species owing to the strong specific hybridization pattern of the H genome. Hexaploid SYH species gave a hybridization pattern similar to SH species for the same reason. The results support the genomic composition of Elymus batalinii as SYP and also indicated the presence of at least one H genome in Elymus enysii with a hitherto unknown genomic constitution. Elymus erianthus had a hybridization pattern distinctly different from all other species in the investigation. Key words : Elymus, RFLP, phylogeny, repetitive DNA.

Molecular characterization and chromosomal distribution of species-specific repetitive DNA sequences from Beta corolliflora, a wild relative of sugar beet

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 1073-1080 ◽  
Author(s):  
D Gao ◽  
T Schmidt ◽  
C Jung
Keyword(s):  
Sugar Beet ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Genomic Dna ◽  
Southern Hybridization ◽  
Repetitive Dna Sequences ◽  
Wild Relative ◽  
Chromosomal Distribution ◽  
Species Specific ◽  
Specific Sequences

Repetitive DNA sequences have been isolated from a Sau3AI plasmid library of tetraploid Beta corolliflora (2n = 4x = 36), a wild relative of sugar beet (B. vulgaris). The library was screened by differential hybridization with genomic DNA of B. corolliflora and B. vulgaris. When used as probes for Southern hybridization of genomic DNA, six clones were determined to represent highly repetitive DNA families present only in the B. corolliflora genome. Five other sequences were highly repetitive in B. corolliflora and low or single copy in B. vulgaris. The insert size varied between 43 bp and 448 bp. Two sequences pBC1279 and pBC1944 displayed strong homology to a previously cloned satellite DNA from B. nana. With one exception, sequences are tandemly arranged as revealed by a typical ladder pattern after genomic Southern hybridization. The chromosomal distribution of five probes was determined by fluorescence in situ hybridization (FISH) of mitotic metaphases from B. corolliflora and a triploid hybrid between B. vulgaris and B. corolliflora. Three sequences were spread along all chromosome arms of B. corolliflora while one sequence was present on only six chromosomes. The chromosome-specific sequence pBC216 was found in close vicinity to the 5S rDNA located on B. corolliflora chromosome IV. This set of species-specific sequences has the potential to be used as probes for the identification of monosomic alien addition lines and for marker-assisted gene transfer from wild beet to cultivated beet.Key words: Beta vulgaris, FISH, repetitive DNA, species-specific sequences.

scholarly journals Repetitive DNA sequences near three humanβ-type globin genes

1980 ◽  
Vol 8 (15) ◽  
pp. 3319-3333 ◽  
Author(s):  
Lesley W. Coggins ◽  
G.Joan Grindlay ◽  
J.Keith Vass ◽  
Alison A. Slater ◽  
Paul Montague ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Repetitive Dna Sequences ◽  
Globin Genes

Fluorescence in situ hybridization on plant extended chromatin DNA fibers for single-copy and repetitive DNA sequences

2011 ◽  
Vol 30 (9) ◽  
pp. 1779-1786 ◽  
Author(s):  
Kun Yang ◽  
Hecui Zhang ◽  
Richard Converse ◽  
Yong Wang ◽  
Xiaoying Rong ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Single Copy ◽  
Repetitive Dna Sequences
Sign in / Sign up

Export Citation Format

Share Document