Characterization of four induced chromosome deficiencies in rice (Oryza sativa L.)

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 307-313 ◽  
Author(s):  
Z. X. Wang ◽  
N. Iwata
Keyword(s):  
Oryza Sativa ◽  
Growth Habit ◽  
Oryza Sativa L ◽  
Rice Genome ◽  
Rice Chromosome ◽  
Marker Genes ◽  
Morphological Marker ◽  
Chromosome Configuration ◽  
Key Words Rice

Four rice (Oryza sativa L.) deficiencies, involving chromosomes 4 (Df4), 8 (Df8), and 11 (Df11-1, Df11-2), were studied. The deficiencies were induced by means of the pseudodominance technique, i.e., strains carrying one or more recessive marker genes were fertilized with irradiated pollen of a strain carrying normal alleles at corresponding loci. No characteristic morphological features were found in the deficiencies, as compared with the normal F1 plants in the progeny. The deficiencies showed high or complete seed sterility. Genetic and cytological studies indicated deficiencies in chromosomes 4, 8, and 11. The fragment chromosomes in Df4, Df8, and Df11-2 were short, possibly being derived from the heterochromatin regions of the chromosomes, including kinetochores, and the fragment chromosome in Df11-1 was long, with about 75% of the long arm missing. At metaphase I, Df4, Df8, and Df11-2 showed only the chromosome configuration of 11 II (bivalents) + 2I (univalents), and Df11-1 only that of 12 II. It seems that the short fragments tend to stay as univalents in meiosis, probably because of their shortness. On the other hand, long fragments act as normal chromosomes and associate with their homologues. The deficiencies were not transmitted to the progenies, although only a few offspring were examined. By using the induced deficiencies Df4 and Df11-1, two morphological marker genes, lg (liguleless) and la (lazy growth habit), were located on the long arm of chromosomes 4 and 11, respectively. This is the first report in rice utilizing induced chromosome deficiencies to locate a gene on a specific arm of a chromosome. The use of induced deficiencies for studying the structure of the rice genome is discussed. Key words : rice, chromosome, deficiencies, cytology, transmission.

scholarly journals Bacteriophage Lambda Genomic Library Construction of Oryza sativa L. var. Pokkali

1970 ◽  
Vol 17 (2) ◽  
pp. 149-159
Author(s):  
Suhaila Rahman ◽  
Masa-aki Ohto ◽  
Zeba I. Seraj ◽  
Eduardo Blumwald
Keyword(s):  
Oryza Sativa ◽  
Salt Tolerance ◽  
Dna Isolation ◽  
Genomic Library ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Rice Chromosome ◽  
Chromosome 1 ◽  
Major Qtl ◽  
Key Words Rice

A major QTL called ‘Saltol’ on rice chromosome 1 has been identified as linked to salinity tolerance traits of the landrace, Pokkali. A genomic library of Pokkali rice variety was constructed in bacteriophage Lamda Fix II with 20 kb inserts. Plaques containing genes implicated to salt tolerance have been identified using probes homologous to S-Ad methionine synthetase (SAM) and cation chloride co-transporter after two rounds of hybridization. Clearly demarcated plaques of the two clones of interest have been lifted out for DNA isolation and subsequent characterization to be followed by subcloning into TAC vectors for Agrobacterium-mediated transformation of large genomic fragments into sensitive rice.  Key words: Rice, Pokkali, Genomic library, Lambda vector, Partial Fill-In, Titer  D.O.I. 10.3329/ptcb.v17i2.3235 Plant Tissue Cult. & Biotech. 17(2): 149-159, 2007 (December)

scholarly journals Comprehensive in silico Characterization of Universal Stress Proteins in Rice (Oryza sativa L.) With Insight Into Their Stress-Specific Transcriptional Modulation

2021 ◽  
Vol 12 ◽  
Author(s):  
Shatil Arabia ◽  
Asif Ahmed Sami ◽  
Saima Akhter ◽  
Rakha Hari Sarker ◽  
Tahmina Islam
Keyword(s):  
Oryza Sativa ◽  
Gene Family ◽  
In Silico ◽  
Stress Proteins ◽  
Oryza Sativa L ◽  
Rice Genome ◽  
Domain Architecture ◽  
Crop Species ◽  
Transcriptional Modulation

In a world where climate change is real and its consequences are unprecedented, understanding of the plant adaptive capacity and native stress-responsive machinery is crucial. In recent years, universal stress proteins (USPs) have received much attention in the field of plant science due to their stress-specific transcriptional regulation. This study focuses on the extensive characterization of the USP gene family members in the monocot crop rice (Oryza sativa L. var. japonica). Here, we report a total of 44 USP genes in the rice genome. In silico characterization of these genes showed that domain architecture played a major role in the functional diversification of the USP gene family which holds for all plant USPs. On top of that, a higher conservation of OsUSP members has been exhibited with a monocot genome (Zea mays L.) as compared to a dicot genome (Arabidopsis thaliana L.). Expression profiling of the identified genes led to the discovery of multiple OsUSP genes that showed pronounced transcript alteration under various abiotic stress conditions, indicating their potential role as multi-functional stress-specific modules. Furthermore, expression validation of OsUSP genes using qRT-PCR provided a strong evidence for the utility OsUSP genes in building multi-stress tolerant plants. Altogether, this study provides leads to suitable USP candidates that could be targeted for plant breeding and genetic engineering experiments to develop stress resilient crop species.

The origin, identification, and plant morphology of five rice monosomics

Genome ◽  
1996 ◽  
Vol 39 (3) ◽  
pp. 528-534 ◽  
Author(s):  
Z. X. Wang ◽  
N. Iwata
Keyword(s):  
Oryza Sativa ◽  
Gamma Irradiation ◽  
Key Words ◽  
Gamma Rays ◽  
Oryza Sativa L ◽  
Plant Morphology ◽  
Marker Genes ◽  
First Time ◽  
Origin Identification ◽  
Key Words Rice

Five different monosomics of rice (Oryza sativa L.) were obtained by treatment of pollen with gamma irradiation, as a by-product of attempts to determine the cytological loci of certain marker genes, i.e., mature pollen carrying normal alleles at all loci was given gamma rays and used for pollinating strains that were homozygous for recessive marker genes. The monosomics showed distinguishable morphological features and had complete seed sterility. Cytological studies revealed that one monosomic was tertiary, the others primary. The tertiary monosomic was related to chromosome 10. Two primary monosomics for chromosomes 10 and 11 were identified. At metaphase I, the tertiary monosomic showed the chromosome configurations 1 III + 10 II, 11 II + 1 I, and 10 II + 3 I, and all primary ones showed the configuration 11 II + 1 I. All five monosomics showed very poor crossing ability and were not transmitted to the few progenies observed. A few trisomic plants were found in the progenies of a cross between monosomic and normal pollen in one monosomic. This is the first time that many monosomics in rice have been characterized. This information will be useful in studies of rice aneuploidy and cytogenetics. Key words : rice, monosomics, morphology, cytology, transmission, trisomics.

Characterization of indica–japonica subspecies-specific InDel loci in wild relatives of rice (Oryza sativa L. subsp. indica Kato and subsp. japonica Kato)

2016 ◽  
Vol 64 (2) ◽  
pp. 405-418 ◽  
Author(s):  
Joong Hyoun Chin ◽  
Yoo-Jin Lee ◽  
Wenzhu Jiang ◽  
Hee-Jong Koh ◽  
Michael J. Thomson
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Wild Relatives

Isolation and characterization of a short lateral root mutant in rice (Oryza sativa L.)

Plant Science ◽  
2003 ◽  
Vol 165 (4) ◽  
pp. 895-903 ◽  
Author(s):  
Bakul Rani Debi ◽  
Junko Mushika ◽  
Shin Taketa ◽  
Akio Miyao ◽  
Hirohiko Hirochika ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Lateral Root ◽  
Oryza Sativa L ◽  
Isolation And Characterization
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