Cytological identification of some trisomics of Russian wildrye (Psathyrostachys juncea)

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1271-1278 ◽  
Author(s):  
Jun-Zhi Wei ◽  
W. F. Campbell ◽  
G. J. Scoles ◽  
A. E. Slinkard ◽  
R. Ruey-Chyi Wang
Keyword(s):  
Crop Improvement ◽  
Diploid Species ◽  
Pollen Grains ◽  
Morphological Characters ◽  
Cereal Crop ◽  
Banding Patterns ◽  
C Banding ◽  
Psathyrostachys Juncea ◽  
Double Deletion ◽  
Russian Wildrye

Russian wildrye, Psathyrostachys juncea (Fisch.) Nevski (2n = 2x = 14; NsNs), is an important forage grass and a potential source of germplasm for cereal crop improvement. Because of genetic heterogeneity as a result of its self-incompatibility, it is difficult to identify trisomics of this diploid species based on morphological characters alone. Putative trisomies (2n = 2x + 1 = 15), derived from open pollination of a triploid plant by pollen grains of diploid plants, were characterized by Giemsa C-banding. Based on both karyotypic criteria and C-banding patterns, four of the seven possible primary trisomics, a double-deletion trisomic, and two tertiary trisomics were identified.Key words: Russian wildrye, Psathyrostachys juncea, trisomic, C-banding, karyotype.

The heterochromatin distribution and genome evolution in diploid species of Elymus and Agropyron

1984 ◽  
Vol 26 (6) ◽  
pp. 669-678 ◽  
Author(s):  
T. Ryu Endo ◽  
Bikram S. Gill
Keyword(s):  
Diploid Species ◽  
Banding Technique ◽  
Agropyron Elongatum ◽  
Pseudoroegneria Spicata ◽  
Banding Patterns ◽  
C Banding ◽  
Psathyrostachys Juncea ◽  
High Level ◽  
Thinopyrum Elongatum ◽  
Genome Designation

The acetocarmine–Giemsa C-banding technique was used to study heterochromatin distribution in somatic chromosomes of diploid Elymus junceus (= Psathyrostachys juncea) (2n = 14) (genome designation Ju = N) and nine diploid Agropyron species (2n = 14): A. cristatum (C = P), A. imbricatum (C = P), A. elongatum (= Elytrigia elongata = Thinopyrum elongatum) (E = J), A. junceum (= E. bessarabicum = T. bessarabicum) (J = E), A. spicatum (= Pseudoroegneria spicata) (S), A. libanoticum (= P. libanotica) (S), A. ferganense (S), A. stipifolium (= P. stipifolia) (S), and A. velutinum (V). With the exception of A. elongatum and A. velutinum, which were self-fertile, all species were cross-pollinating and self-sterile. The cross-pollinating species showed large terminal C-bands and a high level of C-band polymorphism. Agropyron elongatum, moderately self-fertile, showed small terminal and interstitial bands and a minimal C-band polymorphism. Agropyron velutinum, fully self-fertile, almost totally lacked C-bands. The Ju, C, E, and J genomes appeared to be distinctive and the equivalence of the E and J genomes was not supported from their C-banding patterns. Four species sharing the S genome, A. spicatum, A. libanoticum, A. ferganense, and A. stipifolium had C-band patterns similar to one another, although C-bands were less prominent in A. stipifolium than others.Key words: C-banding, karyotype, wheatgrass, cytology.

Standard Giemsa C-banded karyotype of Russian wildrye (Psathyrostachys juncea) and its use in identification of a deletion–translocation heterozygote

Genome ◽  
1995 ◽  
Vol 38 (6) ◽  
pp. 1262-1270 ◽  
Author(s):  
Jun-Zhi Wei ◽  
William F. Campbell ◽  
Richard R.-C. Wang
Keyword(s):  
Banding Pattern ◽  
Geographic Area ◽  
B Chromosome ◽  
Distal Segment ◽  
Banding Technique ◽  
Translocation Heterozygote ◽  
C Banding ◽  
Psathyrostachys Juncea ◽  
Geographical Regions ◽  
Russian Wildrye

Ten accessions of Russian wildrye, Psathyrostachys juncea (Fisch.) Nevski (2n = 2x = 14; NsNs), collected from different geographical regions were analyzed using the C-banding technique. C-banding pattern polymorphisms were observed at all levels, i.e., within homologous chromosome pairs of the same plant, among different individuals within accessions, between different accessions of the same geographic area, and among accessions of different origins. The seven homologous groups varied in the level of C-banding pattern polymorphism; chromosomes A, B, E, and F were more variable than chromosomes C, D, and G. The polymorphisms did not hamper chromosome identification in Ps. juncea, because each chromosome pair of the Ns genome had a different basic C-banding pattern and karyotypic character. A standard C-banded karyotype of Ps. juncea is proposed based on the overall karyotypes and C-bands in the 10 accessions. The C-bands on the Ns-genome chromosomes were designated according to the rules of nomenclature used in wheat. A deletion–translocation heterozygote of Russian wildrye was identified based on the karyotype and C-banding patterns established. The chromosome F pair consisted of a chromosome having the distal segment in the long arm deleted and a translocated chromosome having the distal segment of long arm replaced by the distal segment of the long arm of chromosome E. The chromosome E pair had a normal chromosome E and a translocated chromosome having the short arm and the proximal segment of the long arm of chromosome E and the distal segment of the long arm of chromosome F.Key words: Psathyrostachys juncea, karyotype, Giemsa C-banding, polymorphism, B chromosome.

Giemsa C-banded karyotypes of Avena species

Genome ◽  
1988 ◽  
Vol 30 (5) ◽  
pp. 627-632 ◽  
Author(s):  
A. Fominaya ◽  
C. Vega ◽  
E. Ferrer
Keyword(s):  
Interspecific Hybrids ◽  
Chromatin Condensation ◽  
Diploid Species ◽  
Divergent Evolution ◽  
Intercalary Heterochromatin ◽  
Banding Patterns ◽  
Genome Species ◽  
C Banding ◽  
A Genome ◽  
C Genome

Giemsa C-banding was used to identify individual somatic chromosomes in eight diploid species of Avena. Two patterns of heterochromatin distribution were found. The chromosomes of five A genome species (A. strigosa, A. hirtula, A. longiglumis, A. damascena, and A. canariensis) possessed mainly telomeric bands, whereas those from three C genome species (A. clauda, A. pilosa, and A. ventricosa) were characterized by higher chromatin condensation and several intercalary heterochromatin bands. The divergent evolution between the two groups is confirmed after C-banding. The unique C-banding patterns of several chromosomes in each species will be a useful tool for the study of meiotic behaviour in interspecific hybrids among Avena spp.Key words: C-banding, Avena, heterochromatin.

Analysis of karyotypes and Giemsa C-banding patterns in eight species of Arachis

Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 187-194 ◽  
Author(s):  
Q. Cai ◽  
S. Lu ◽  
C. C. Chinnappa
Keyword(s):  
Close Relationships ◽  
Diploid Species ◽  
Chromosome Morphology ◽  
Tetraploid Species ◽  
Interspecific Relationship ◽  
Banding Patterns ◽  
C Banding ◽  
B Genome ◽  
A Genome ◽  
Structural Differences

The karyotypes and Giemsa C-banding patterns of the chromosomes in eight species of Arachis L. have been studied. Six species are diploid with 20 chromosomes and two are tetraploid with 40 chromosomes. One diploid species (A. rigonii Krap. et Greg.) belongs to the sect. Erectoides and the rest belong to the sect. Arachis. Among the diploid species from the sect. Arachis, A. batizocoi Krap. et Greg, has a unique karyotype while others have similar karyotypes. Two tetraploid species, A. monticola Krap. et Greg, and A. hypogaea L., possess the most similar karyotypes. However, the diploid species, A. rigonii, from sect. Erectoides, has a karyotype distinguishable from those in sect. Arachis. The C-banding patterns of the chromosomes have been obtained for all the species. The centromeric bands have been found in all the chromosomes and the intercalary bands can be identified in a varied number of chromosomes among these complements. However, the telomeric bands only exist in one or two chromosomes. The comparison of banding patterns demonstrated that structural differences exist among the chromosomal complements of the species with similar chromosome morphology. The karyotype variation among the different species and interspecific relationship are discussed. It is suggested that all the diploid species with the A genome are closely related. There are close relationships between the tetraploid species and diploid species with the A or B genome within sect. Arachis. Key words: Arachis, cytology, karyotypes, Giemsa C-banding.

C-banding and nucleolar activity of tetraploid Avena species

Genome ◽  
1988 ◽  
Vol 30 (5) ◽  
pp. 633-638 ◽  
Author(s):  
A. Fominaya ◽  
C. Vega ◽  
E. Ferrer
Keyword(s):  
Diploid Species ◽  
Nucleolar Organizer ◽  
Nucleolar Organizer Regions ◽  
Good Correspondence ◽  
Banding Patterns ◽  
Genome Species ◽  
Nucleolar Activity ◽  
C Banding ◽  
A Genome ◽  
Nucleolar Organizer Activity

The Giemsa C-banding pattern of the chromosomes of five tetraploid species of Avena have been studied. The chromosomes of AABB species (A. barbata, A. vaviloviana, and A. abyssinica) had similar C-banding patterns to those of A genome species. AACC species (A. maroccana and A. murphyi) possessed two sets of seven chromosome pairs with C-banding patterns similar to those observed in the diploid A and C genome species. However, no good correspondence between either of these two chromosome groups and any one diploid species has been found. When the nucleolar organizer activity of the species was analysed by silver staining, fewer nucleoli and nucleolar organizer regions (NORs) were observed than expected, assuming complete additivity of those from the donor diploid species.Key words: C-banding, NOR, Avena, heterochromatin.

Genome analysis of Elytrigia caespitosa, Lophopyrum nodosum, Pseudoroegneria geniculata ssp. scythica, and Thinopyrum intermedium (Triticeae: Gramineae)

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 102-111 ◽  
Author(s):  
Zhi-Wu Liu ◽  
Richard R.-C. Wang
Keyword(s):  
Karyotype Analysis ◽  
Diploid Species ◽  
Pollen Grains ◽  
Root Tip ◽  
Meiotic Pairing ◽  
Tetraploid Species ◽  
Thinopyrum Intermedium ◽  
Male Sterile ◽  
Stainable Pollen ◽  
C Banding

To elucidate the genome constitutions of the tetraploid (2n = 4x = 28) species Elytrigia caespitosa, Lophopyrum nodosum, and Pseudoroegneria geniculata ssp. scythica and the hexaploid (2n = 6x = 42) Thinopyrum intermedium, meiotic pairing was studied in these species as well as 10 hybrids. Karyotype analysis with aceto-orcein stained root-tip cells was performed for the four species and the hybrids of T. bessarabicum with E. caespitosa, P. geniculata ssp. scythica, and T. intermedium. Karyotype analysis by Giemsa C-banding was carried out with the three tetraploid species and the two triploid hybrids involving T. bessarabicum. The species behaved as strict allopolyploids. All hybrids were male sterile with few stainable pollen grains. It is concluded from the results that the three tetraploid species have the genome formula JeJeSS and T. intermedium has the formula JeJeJeJeSS. The chromosomes of the Je and S genomes in these species had C-banding patterns differing from each other and from those of the extant diploid species. Based on these findings, the four species investigated should be placed in the same genus or the same section of a genus. However, new combinations are not proposed at this time pending future taxonomic investigation of the genome constitution of Elytrigia repens (L.) Nevski.Key words: genome, hybrid, meiosis, karyotype, chromosome banding, speciation.

Comparative Analysis of Genetic and Morphological Variation within the Platanthera hyperborea Complex (Orchidaceae)

2020 ◽  
Vol 45 (4) ◽  
pp. 767-778
Author(s):  
Eranga Wettewa ◽  
Nick Bailey ◽  
Lisa E. Wallace
Keyword(s):  
North America ◽  
Diploid Species ◽  
Morphological Characters ◽  
Evolutionary Patterns ◽  
Founding Population ◽  
Multiple Origins ◽  
Geographic Structure ◽  
Species Complexes ◽  
Nuclear Its ◽  
Cryptic Taxa

Abstract—Species complexes present considerable problems for a working taxonomy due to the presence of intraspecific variation, hybridization, polyploidy, and phenotypic plasticity. Understanding evolutionary patterns using molecular markers can allow for a more thorough assessment of evolutionary lineages than traditional morphological markers. In this study, we evaluated genetic diversity and phylogenetic patterns among taxa of the Platanthera hyperborea (Orchidaceae) complex, which includes diploid (Platanthera aquilonis) and polyploid (Platanthera hyperborea, P. huronensis, and P. convallariifolia) taxa spanning North America, Greenland, Iceland, and Asia. We found that three floral morphological characters overlap among the polyploid taxa, but the diploid species has smaller flowers. DNA sequence variation in a plastid (rpL16 intron) and a nuclear (ITS) marker indicated that at least three diploid species have contributed to the genomes of the polyploid taxa, suggesting all are of allopolyploid origin. Platanthera convallariifolia is most like P. dilatata and P. stricta, whereas P. huronensis and P. hyperborea appear to have originated from crosses of P. dilatata and P. aquilonis. Platanthera huronensis, which is found across North America, has multiple origins and reciprocal maternal parentage from the diploid species. By contrast, P. hyperborea, restricted to Greenland and Iceland, appears to have originated from a small founding population of hybrids in which P. dilatata was the maternal parent. Geographic structure was found among polyploid forms in North America. The area of Manitoba, Canada appears to be a contact zone among geographically diverse forms from eastern and western North America. Given the geographic and genetic variation found, we recommend continued recognition of four green-flowered species within this complex, but caution that there may be additional cryptic taxa within North America.

scholarly journals Diversity of Common Bean Landraces Collected in the Western and Eastern Carpatien

2011 ◽  
Vol 39 (No. 3) ◽  
pp. 73-83 ◽  
Author(s):  
O. Horňáková ◽  
M. Závodná ◽  
M. Žáková ◽  
J. Kraic ◽  
F. Debre
Keyword(s):  
Cluster Analysis ◽  
Common Bean ◽  
Morphological Characters ◽  
Molecular Data ◽  
Seed Traits ◽  
Phaseolus Vulgaris L ◽  
Growth Type ◽  
Banding Patterns ◽  
Polymorphism Detection ◽  
Thousand Seed Weight

The study of diversity in common bean was based on morphological and agronomical characteristics, differentiation of collected accessions by morphological and molecular markers, detection of genetic variation, and duplicates detection in bean landraces. The analysed 82 accessions of common bean (Phaseolus vulgaris L.) were collected in the Western andEastern Carpatien as landrace mixtures. Their seeds were segregated and pooled according to their characteristics; they were further multiplicated, and introduced into the collection. An extensive variation in plant and seed traits was discovered in thirty-three morphological and agronomical characteristics. Nevertheless, some of the accessions were identical in these characteristics. Cluster analysis grouped genotypes into two main branches, reflecting the growth type, seed size parameters, and thousand-seed weight. Molecular differentiation studies were performed by multilocus polymorphism detection in microsatellite and minisatellite DNA regions. Cluster analysis based on molecular data also grouped genotypes but no linkage to morphological traits was revealed. Bean accessions with very similar or identical morphological characters were clearly distinguished by DNA banding patterns. The presence of duplicates was excluded.  

Rye C-banding patterns and meiotic stability of hexaploid triticale (× Triticosecale) selections differing in kernel shriveling

Genome ◽  
1990 ◽  
Vol 33 (5) ◽  
pp. 686-689 ◽  
Author(s):  
Charles M. Papa ◽  
R. Morris ◽  
J. W. Schmidt
Keyword(s):  
Secale Cereale ◽  
Chromosome Banding ◽  
Agronomic Performance ◽  
Hexaploid Triticale ◽  
Kernel Development ◽  
Banding Patterns ◽  
C Banding ◽  
Meiotic Stability ◽  
Metaphase I

Two winter hexaploid triticale populations derived from the same cross were selected on the basis of grain appearance and agronomic performance. The five lines from 84LT402 showed more kernel shriveling than the four lines from 84LT401. The derived lines were analyzed for aneuploid frequencies, rye chromosome banding patterns, and meiotic stability to detect associations with kernel development. The aneuploid frequencies were 16% in 84LT401 and 18% in 84LT402. C-banding showed that both selection groups had all the rye chromosomes except 2R. The two groups had similar telomeric patterns but differed in the long-arm interstitial patterns of 4R and 5R. Compared with lines from 84LT402, those from 84LT401 had significantly fewer univalents and rod bivalents, and more paired arms at metaphase I; fewer laggards and bridges at anaphase I; and a higher frequency of normal tetrads. There were no significant differences among lines within each group for any meiotic character. Since there were no differences within or between groups in telomeric banding patterns, the differences in kernel shriveling and meiotic stability might be due to genotypic factors and (or) differences in the interstitial patterns of 4R and 5R. By selecting plump grains, lines with improved kernel characteristics along with improved meiotic stability are obtainable.Key words: triticale, meiotic stability, C-banding, Secale cereale, heterochromatin.

A natural intergeneric hybrid of Gesneriaceae from Brazil

Phytotaxa ◽  
2021 ◽  
Vol 497 (2) ◽  
pp. 79-96
Author(s):  
ANDRÉA ONOFRE DE ARAUJO ◽  
MAURO PEIXOTO ◽  
CINTIA NEVES DE SOUZA ◽  
EDUARDO CUSTÓDIO GASPARINO ◽  
JULIANA TOLEDO FARIA ◽  
...  
Keyword(s):  
Chromosome Numbers ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Intergeneric Hybrid ◽  
Morphological Characters ◽  
Natural Hybrid ◽  
Pollen Data ◽  
Cytological Data ◽  
High Viability ◽  
Structural Abnormalities

A natural hybrid between Goyazia and Mandirola (Gloxiniinae, Gesneriaceae) from Cerrado (Brazil) is here described, supported by pollen morphology, cytological data and morphological characters. The microsporogenesis of Mandirola hirsuta and that of the hybrid were analyzed in order to evaluate the cytogenetic characteristics. The haploid chromosome numbers observed were n = 12 for M. hirsuta and n = 11, 13, 16 and 26 for the hybrid. Structural abnormalities (monads, dyads, triads and micronuclei) were observed at the final of the hybrid’s meiosis. High viability rates of the pollen were recorded for Goyazia and Mandirola (>90%) and low viability for the hybrid (34.7%). The pollen grains were acetolyzed, measured and photographed for pollen morphology analysis. Quantitative pollen data were analyzed through descriptive and multivariate statistics. The hybrid has intermediate pollen characteristics between G. petraea and M. hirsuta; it is more related to G. petraea by the measures of diameters and ectoapertures; it is more similar to M. hirsuta mainly regarding the microreticulum on the mesocolpium region. The hybrid and Mandirola share vegetative and flower size, while the colors of the hybrid are similar to Goyazia. Pollen morphology, cytological data and morphological characters brought clear evidence for the recognition of the intergeneric hybrid, which we named as Goydirola x punctata.

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